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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (01): 50-54.doi: 10.3724/SP.J.1006.2012.00050

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Factors Affected BC1F1 Size for Development of Genome-wide Introgression Lines

YAN Long1,2,LIU Hui-Yong1,2,LI Ying-Hui1,ZHANG Meng-Chen2,QIU Li-Juan1,*   

  1. 1 National Key Facility of Crop Gene Resource and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 2 Institute of Cereal and Oil Crops, Hebei Academy of Agricultural and Forestry Sciences / Shijiazhuang Branch Center of National Center for Soybean Improvement / Key Laboratory of Crop Genetics and Breeding, Shijiazhuang 050031, China
  • Received:2011-03-10 Revised:2011-06-13 Online:2012-01-12 Published:2011-11-07
  • Contact: QIU Li-Juan, E-mail: qiu_lijuan@263.net
  • Supported by:

    The Research Supported by State Key Basic Research and Development Plan of China (973 program) (2004CB117203) and International Science and Technology Cooperation and Exchanges Projects (2008DFA30550).

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Abstract: Introgression lines are important genetic materials for genetics study and breeding. Development of those lines involves cross and backcross processes between recipient and donor parents. The population size of BC1F1 is a critical parameter for fully covering donor genome and successfully obtaining desired introgression lines. However, the minimum sufficient number of BC1F1 plants is unknown for each species and can not be obtained experimentally. We have developed a computer program by simulating the recombination process during meiosis to define the ideal BC1F1 population size. The reliability of the program was confirmed by mathematics and experimental data. Three factors including linkage groups number, linkage group length and gene density were analyzed and all of them had positive relation with the BC1F1 population size. The population size increased from 6.06 to 9.49 when the linkage number increased from 5 to 40. The population size was 7.14 when the linkage group length was 80 cM, while it became 8.64 when the length was 200 cM. The population size was 7.65 with the density of 20 cM per gene and 8.22 with 10 cM per gene. The BC1F1 population sizes of rice, wheat, maize and soybean were predicted to be 12, 13, 14–15, and 13 by the program with 95% confident level.

Key words: Introgression lines, Population size, Simulate

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