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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (01): 99-106.doi: 10.3724/SP.J.1006.2012.00099

• TILLAGE & CULTIVATION · PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Response of Some Key Enzyme Activities Involved in Nitrogen Metabolism to High Temperature at Filling Stage and Its Relation to Storage Protein Accumulation in Rice Grain

CAO Zhen-Zhen,ZHANG Qi-Fang,WEI Ke-Su,YANG Wei-Li,LIU Gaung-Kuai,CHENG Fang-Min*   

  1. College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China
  • Received:2011-05-20 Revised:2011-10-12 Online:2012-01-12 Published:2011-11-07
  • Contact: 程方民, E-mail: chengfm@zju.edu.cn

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Abstract: The influence of high temperature after flowering period on the activities of some enzymes, including glutamine synthetase (GS), glutamic-oxaloacetic transaminase (GOT)and glutamic-pyruvic transaminase (GPT) in developing grains, and also its relation to grain storage protein and amino acid accumulations were investigated by using two early-indica rice cultivars grown under two temperature regimes of daily average temperature 32℃ and 22℃, respectively, and expression responses of two GS isoform genes to temperature were detected by real-time fluorescence quantitative PCR(FQ-PCR). The results indicated that the effects of high temperature after flowering period on GOT and GPT activities showed the similar trend, with increasing at early grain filling stage and decreasing at late grain filling stage. However, the increase f total contents of crude protein and various amino acids under high temperature after flowering period were not always due to the enhancement of grain nitrogen transport capacity and protein synthesis ability. GS activity under high temperature was generally higher than that under temperature in the same period. GS2,one of the isoforms of GS gene, was highly expressed in rice endosperm. The expression levels of GS2 was even higher than that of GS1 in late grain filling stage, while high temperature stress regulated GS activity through changing GS1 and GS2 genes transcription level in grain.

Key words: Rice (Oryza sativa L.), High temperature, Nitrogen metabolism, Enzyme activity, Protein accumulation

[1]Peng S B, Huang J L, Sheehy J E, Laza R C, Visperas R M, Zhong X H, Centeno G S, Khush G S, Cassman K G. Rice yields decline with higher night temperature from global warming. Proc Natl Acad Sci USA, 2004, 101: 9971–9975
[2]Prasad P V, Boote K J, Allen L H, Sheehy J E, Thomas L M G. Species, ecotye and cultivar differences in spikelet fertility and harvest index of rice in response to high temperature stress. Field Crops Res, 2006, 95: 398–411
[3]Tashiro T, Warldaw F. The effect of high temperature on kernel dimension and the type and occurrence of kernel damage in rice. Aust J Agric Res, 1991, 42: 485–496
[4]Zhong L J, Cheng F M, Sun Z X, Zhang G P. The deterioration of eating and cooking quality caused by high temperature during grain filling in early–season indica rice cultivars. J Agron Crop Sci, 2005, 191: 218–225
[5]Cheng F M, Zhong L J, Zhao NC, Liu Y, Zhang G P. Temperature induced changes in the starch components and biosynthetic enzymes of two rice varieties. Plant Growth Regul, 2005, 46:87–95
[6]Li M-Y(李木英), Shi Q-H(石庆华), Hu Z-H(胡志红), Pan X-H(潘晓华), Tan X-M(谭雪明). Effects of high temperature stress on activity of amylosynthease in endosperm of early indica rice varieties. Sci Agric Sin(中国农业科学), 2007, 40(8): 1622–1629(in Chinese with English abstract)
[7]Zakaria S, Mastuda T, Tajima S, Nitta Y. Effect of high temperature at ripening stage on the reserve accumulation in seed in some rice cultivars. Plant Prod Sci, 2002, 5: 160–168
[8]Huang Y-J(黄英金), Qi Y-X(漆映雪), Liu Y-B(刘宜柏), Chen D-Z(陈大洲). Effect of climatic factors on the contents of protein and four protein fractions in early rice during the milking and mature period. Chin J Agromet(中国农业气象), 2002, 23(2): 54–59 (in Chinese with English abstract)
[9]Liang C-G(梁成刚), Chen L-P(陈利平), Wang Y(汪燕), Liu J(刘佳), Xu G-L(许光利), Li T(李天). Effects of high temperature on key enzyme activities of nitrogen metabolism and protein. Chin J Rice Sci (中国水稻科学), 2010, 24(4): 398–402 (in Chinese with English abstract)
[10]Lea P J, Ireland R J. Plant amino acids. In: Singh B K. Nitrogen Metabolism in Higher Plants. New York: Marcel Dekker, 1999. pp 1–47
[11]Yamagata H, Tanaka K. The site of synthesis and accumulation of rice storage proteins. Plant Cell Physiol, 1986, 27: 135–145
[12]Zhang Z-L(张志良), Qu W-J(瞿伟菁). Physiology of Plant Experimental Guidance, 3rd edn (植物生理实验指导). Beijing: Higher Education Press, 2005. pp 167–169 (in Chinese)
[13]Zhang H-Y(张海燕), Dong H-T(董海涛), Yao H-G(姚海根), Xiang Y-W(向跃武), Tan X-L(谭学林), Li D-B(李德葆). Isolation of total RNA from rice endosperm and expression of important genes related to grain quality in rice (Oryza sativa L.). Chin J Rice Sci (中国水稻科学), 2005, 19(2): 105–110 (in Chinese with English abstract)
[14]Rajeevan M S, Ranamukhaarachi D G, Vernon S D, Unger E R. Use of real-time quantitative PCR to validate the results of cDNA array and differential display PCR technologies. Methods, 2001, 25: 443–451
[15]Zou Q(邹琦). Physiology of Plant Experimental Guidance (植物生理学实验指导). Beijing: Chinese Agriculture Press, 2000. pp 129–130 (in Chinese)
[16]National Standard of PR China (中华人民共和国国家标准). Pretreatment Method for Determination of Amino Acids in Cereal Grains (谷物氨基酸测定的预处理方法) (GB7649-87). Beijing: Standards Press of China, 1987. pp 314–315 (in Chinese)
[17]Quan Q-Z(权清转), Chen Z-J(陈志杰), Zhang H-L(张赫莲), Liang Y-L(梁银丽), Zhang F(张锋). Study on amino acid composition of bagging cucumber in greenhouse. Amino Acids & Biotic Res Resour, 2004, 26: 12–13 (in Chinese with English abstract)
[18]Lam H M, Coschigano K T, Oliveira I C, Oliveria R M, Coruzzi G M. The Molecular genetics of nitrogen assimilation into amino acids in higher plants. Annu Rev Plant Physiol Biol, 1996, 47: 569–593
[19]Tashiro T, Warldaw F. The effect of high temperature on kernel dimension and the type and occurrence of kernel damage in rice. Aust J Agric Res, 1991, 42: 485–496
[20]Hamaker B R, Griffin V K. Effect of disulfide bond-containing protein on rice starch gelatinization and pasting. Cereal Chem, 1993, 70: 377–380
[21]Xiao H-H(肖辉海), Wang W-L(王文龙), Hao X-H(郝小花). Effects of high temperature on key enzyme activities related to nitrogen metabolism and protein content of early indica rice grain. Jiangsu J Agric Sci (江苏农业学报), 2010, 26(4):680–685(in Chinese with English abstract)
[22]Tao L-X(陶龙兴), Wang X(王熹), Liao X-Y(廖西元), Shen B(沈波), Tan H-J(谭慧娟), Huang S-W(黄世文). Effects of air temperature and sink-source strength on rice quality and some physiological traits. Chin J Appl Ecol(应用生态学报), 2006, 17(4): 647–652 (in Chinese with English abstract)
[23]Wei K-S(韦克苏), Cheng F-M(程方民), Dong H-T(董海涛), Zhang Q-F(张其芳), Liu K-G(刘奎刚), Cao Z-Z(曹珍珍). Microarray analysis of gene expression profile to grain storage metabolism in rice endosperm as affected by high temperature at filling stage. Sci Agric Sin (中国农业科学), 2010, 43(1): 1–11 (in Chinese with English abstract)
[24]Zhou G-Q(周广洽), Xu M-L(徐孟亮), Tan Z-Z(谭周镃), Li X-Z(李训贞). Effects of ecological factors on protein and amino acids in rice. Acta Ecol Sin (生态学报), 1997, 17(5): 537–542 (in Chinese with English abstract)
[25]Ma Q-L(马启林), Li Y-S(李阳生), Tian X-H(田小海), Yan S-Z(鄢圣之), Lei W-C(雷慰慈), Nakata N. Influence of high temperature stress on composition and accumulation configuration of storage protein in rice. Sci Agric Sin (中国农业科学), 2009, 42(2): 714–718 (in Chinese with English abstract)
[26]Miflin B, Habash D. The role of glutamine synthetase and glutamate dehydrogenase in nitrogen assimilation and possibilities for improvement in the nitrogen utilization of crops. J Exp Bot, 2002, 53: 979–987
[27]Daubresse C M, Carrayol E, Valadier M H. The two nitrogen mobilisation and senescence-associated GS1 and GDH genes are controlled by C and N metabolites. Planta, 2005, 221: 580–588
[28]Tang X-R(唐湘如), Guan C-Y(官春云), Yu T-Q(余铁桥). The relationship between rice yielding, grain quality and substance metabolism. J Hunan Agric Univ (湖南农业大学学报), 1999, 25(4): 279–282 (in Chinese with English abstract)
[29]Tabuchi M, Abiko T, Yamaya T. Assimilation of ammonium ions and reutilization of nitrogen in rice (Oryza sativa L.). J Exp Bot, 2007, 58: 2319–2327
[30]Dubois F, Brugiere N, Sangwan R S, Hirel B. Localization of tobacco cytosolic glutamine synthetase enzymes and the corresponding transcripts shows organ and cell-specific patterns of protein synthesis and gene expression. Plant Mol Biol, 1996, 31: 803–817
[31]Ochs G, Schoth G, Trischler M, Kosemund K, Wild A. Complexity and expression of the glutamine synthetase multigene family in the amphidiploid crop Brassica napus. Plant Mol Biol, 1999, 39: 395–405
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