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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (04): 732-739.doi: 10.3724/SP.J.1006.2012.00732

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Effect of Chilling Stress on Physiological Metabolism in Chloroplasts of Seedlings of Sugarcane Varieties with Different Chilling Resistance

SUN Fu1,3,YANG Li-Tao1,*,XIE Xiao-Na1,LIU Guang-Ling1,LI Yang-Rui1,2,3,*   

  1. 1 Agricultural College / State Key Laboratory of Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, Nanning 530005, China; 2 Guangxi Academy of Agricultural Sciences, Nanning 530007, China; 3 Sugarcane Research Center, Chinese Academy of Agricultural Sciences / Guangxi Crop Genetic Improvement and Biotechnology Lab / Guangxi Key Laboratory of Sugarcane Genetic Improvement, Nanning 530007, China.
  • Received:2011-07-27 Revised:2011-12-19 Online:2012-04-12 Published:2012-02-13
  • Contact: 杨丽涛, E-mail: litao61@hotmail.com; 李杨瑞, E-mail: liyr@gxaas.net

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Abstract: Chilling occurs frequently in recent years, and has become a major limiting factor for sugarcane production. Chloroplasts are the main organelle for photosynthesis and one of the subcellular fractions for chilling sensitivity in plants. In order to investigate the effect of chilling stress on the physiological metabolism of sugarcane chloroplast, and reveal the relationships between the physiological characteristics in chloroplast and chilling tolerance of sugarcane, the experiment was conducted with two varieties of GT28 (chilling resistant) and ROC22 (chilling susceptible) with two treatments: control (28°C) and chilling stress (4°C). The relative electrical conductance (REC) of sugarcane seedling leaves, and chlorophyll fluorescence parameters and some physiological indiees in chloroplasts were measured. The results showed that under the low temperature stress condition, the plasma membrane permeability of the seedling leaves, initial fluorescence (Fo) and NPQ increased significantly in both varieties, and the chilling resistant variety GT28 was more stable than the chilling sensitive variety ROC22; and theFv/Fm, Fv'/Fm', qP, and ΦPSII decreased in the two sugarcane varieties, and decreased less in GT28 than in ROC22. The activities of Hill reaction, Mg2+-ATPase and Ca2+-ATPase in chloroplasts decreased, and those in GT28 decreased less than those in ROC22. The MDA content and SOD, POD, GR activities in the chloroplasts increased, and sugarcane variety GT28 showed lower increase in MDA but higher increase in SOD, POD, GR activities. The chlorophyll fluorescence parameters and the physiological characteristics in the chloroplasts of sugarcane leaves were affected significantly by chilling stress. The changes of chlorophyll fluorescence and the physiological characteristics in the chloroplasts are correlated significantly to low temperature resistance, and GT28 is more chilling resistant than ROC22.

Key words: Sugarcane (Saccharum officenarum L), Chilling stress, Chloroplast, Chlorophyll fluorescence, ATPase, ROS

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