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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (04): 732-739.doi: 10.3724/SP.J.1006.2012.00732

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Effect of Chilling Stress on Physiological Metabolism in Chloroplasts of Seedlings of Sugarcane Varieties with Different Chilling Resistance

SUN Fu1,3,YANG Li-Tao1,*,XIE Xiao-Na1,LIU Guang-Ling1,LI Yang-Rui1,2,3,*   

  1. 1 Agricultural College / State Key Laboratory of Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, Nanning 530005, China; 2 Guangxi Academy of Agricultural Sciences, Nanning 530007, China; 3 Sugarcane Research Center, Chinese Academy of Agricultural Sciences / Guangxi Crop Genetic Improvement and Biotechnology Lab / Guangxi Key Laboratory of Sugarcane Genetic Improvement, Nanning 530007, China.
  • Received:2011-07-27 Revised:2011-12-19 Online:2012-04-12 Published:2012-02-13
  • Contact: 杨丽涛, E-mail: litao61@hotmail.com; 李杨瑞, E-mail: liyr@gxaas.net

Abstract: Chilling occurs frequently in recent years, and has become a major limiting factor for sugarcane production. Chloroplasts are the main organelle for photosynthesis and one of the subcellular fractions for chilling sensitivity in plants. In order to investigate the effect of chilling stress on the physiological metabolism of sugarcane chloroplast, and reveal the relationships between the physiological characteristics in chloroplast and chilling tolerance of sugarcane, the experiment was conducted with two varieties of GT28 (chilling resistant) and ROC22 (chilling susceptible) with two treatments: control (28°C) and chilling stress (4°C). The relative electrical conductance (REC) of sugarcane seedling leaves, and chlorophyll fluorescence parameters and some physiological indiees in chloroplasts were measured. The results showed that under the low temperature stress condition, the plasma membrane permeability of the seedling leaves, initial fluorescence (Fo) and NPQ increased significantly in both varieties, and the chilling resistant variety GT28 was more stable than the chilling sensitive variety ROC22; and theFv/Fm, Fv'/Fm', qP, and ΦPSII decreased in the two sugarcane varieties, and decreased less in GT28 than in ROC22. The activities of Hill reaction, Mg2+-ATPase and Ca2+-ATPase in chloroplasts decreased, and those in GT28 decreased less than those in ROC22. The MDA content and SOD, POD, GR activities in the chloroplasts increased, and sugarcane variety GT28 showed lower increase in MDA but higher increase in SOD, POD, GR activities. The chlorophyll fluorescence parameters and the physiological characteristics in the chloroplasts of sugarcane leaves were affected significantly by chilling stress. The changes of chlorophyll fluorescence and the physiological characteristics in the chloroplasts are correlated significantly to low temperature resistance, and GT28 is more chilling resistant than ROC22.

Key words: Sugarcane (Saccharum officenarum L), Chilling stress, Chloroplast, Chlorophyll fluorescence, ATPase, ROS

[1]Li Y-R(李杨瑞), Yang L-T(杨丽涛). New developments in sugarcane industry and technologies in China since 1990s. Southwest China J Agric Sci (西南农业学报), 2009, 22(5): 1469-1476 (in Chinese with English abstract)

[2]Zhou M-L(周美丽), Lu J(陆甲), Li Y-L(李艳兰), Huang X-S(黄雪松). Climatic Characteristic and its impact assessment of Guangxi in 2008. J Meteorol Res Appl (气象研究与应用), 2009, 30(2): 62-65 (in Chinese with English abstract)

[3]Tan H-W(谭宏伟), Li Y-R(李杨瑞), Zhou L-Q(周柳强), Xie R-L(谢如林), Huang J-S(黄金生), Huang M-F(黄美福). Influences of frosty weather on sucrose content and growth of main sugarcane varieties in the central areas of Guangxi. Guangxi Agric Sci (广西农业科学), 2010, 41(4): 326-328 (in Chinese with English abstract)

[4]Chen S-N(陈善娜), Zou X-J(邹晓菊), Liang B(梁斌). Electronmicroscope observation on membrane system of leave cells of some varieties of rice seeding with different cold-resistance. Plant Physiol Commun (植物生理学通讯), 1997, 33(3): 191-194 (in Chinese)

[5]Wu J-C(吴锦程), Liang J(梁杰), Chen J-Q(陈建琴), Dai Q-B(戴巧斌), Cao L-H(曹连黄), Xu X(许鑫), Xu J-B(许金榜), Guan L(官玲). Effects of GSH on AsA-GSH circulation metabolism in chloroplasts of young loquat fruits under low temperature stress. Sci Silv Sin (林业科学), 2009, 45(11): 15-19 (in Chinese with English abstract)

[6]Fu L-S(付连双), Wang X-N(王晓楠), Wang X-D(王学东), Xie F-D(谢甫绨), Li Z-F(李卓夫). Comparison of cell ultrastructure between winter wheat cultivars during cold acclimation and freezing period. J Triticeae Crops (麦类作物学报), 2010, 30(1): 66-72 (in Chinese with English abstract)

[7]Kudoh H, Sonike K. Irreversible damage to photosystem I by chilling in the light: cause of the degradation of chlorophyll after returning to normal growth temperature. Planta, 2002, 215: 541-548

[8]Chen Q-J(陈青君), Zhang F-M(张福墁). Photosynthetic characters of different cucumber cultivars under low temperature and poor light stress and during the recovery. J China Agric Univ (中国农业大学学报), 2000, 5(5): 30-35 (in Chinese with English abstract)

[9]Zhang M-Q(张木清), Chen R-K(陈如凯), Lü J-L(吕建林), Luo J(罗俊), Xu J-S(徐景升). Effects of low temperature stress on the chlorophyll a fluorescence induction kinetics in the seedling of sugarcane. J Fujian Agric Univ (福建农业大学学报), 1999, 28(1): 1-7 (in Chinese with English abstract)

[10]Byrd G T, Ort D R, Ogren W L. The effects of chilling in the light on ribulose-1,5-bisphosphate carboxylase/oxygenase activation in tomato (Lycopersicon esculentum Mill.). Plant Physiol, 1995, 107: 585-591

[11]Sun J(孙锦), Jia Y-X(贾永霞), Guo S-R(郭世荣), Li J(李娟). Effects of seawater stress on metabolism of reactive oxygen species and chlorophyll in chloroplasts of spinach (Spinacia olerancea L). Acta Ecol Sin (生态学报), 2009, 29(8): 4361-4371 (in Chinese with English abstract)

[12]Gamble P E, Burke J J. Effect of water stress on the chloroplast antioxidant system I: alterations in glutathione reductase activity. Plant Physiol, 1984, 76: 615-621

[13]Hua C(华春), Wang R-L(王仁雷), Liu Y-L(刘友良). Effect of exogenous ascorbic acid on active oxygen scavenging system in chloroplasts of rice under salt stress. Acta Agron Sin (作物学报), 2004, 30(7): 692-696 (in Chinese with English abstract)

[14]You J-H(由继红), Lu J-M(陆静梅), Yang W-J(杨文杰). Effects of Ca2+ on photosynthesis and related physiological indexes of wheat seedlings under low temperature stress. Acta Agron Sin (作物学报), 2002, 28: 693-696 (in Chinese with English abstract)

[15]Sun F(孙富), Huang Q-L(黄巧玲), Huang X(黄杏), Zhang B-Q(张保青), Chen M-H(陈明辉), Yang L-T(杨丽涛), Li Y-R(李杨瑞). Isolation of chloroplast from leaf tissue of sugarcane and extraction of their protein. J Southern Agric (南方农业学报), 2011, 42(5): 463-467 (in Chinese with English abstract)

[16]Zhang Y-S(张以顺), Huang X(黄霞), Chen Y-F(陈云凤). Plant Physiology Experiments Tutorial (植物生理学实验教程). Beijing: Higher Education Press, 2009. pp 128-129 (in Chinese)

[17]Li C-N(李长宁), Srivastava M K, Nong Q(农倩), Li Y-R(李杨瑞). Mechanism of tolerance to drought in sugarcane plant enhanced by foliage dressing of abscisic acid under water stress. Acta Agron Sin (作物学报), 2010, 36(5): 863-870 (in Chinese with English abstract)

[18]Tang Z-C(汤章城). Modern Plant Physiology Experiments Guide (现代植物生理学实验指南). Beijing: Science Press, 1999. pp 1-109 (in Chinese)

[19]Xue Y-L(薛应龙). Experimental Manual of Plant Physiology (植物生理学实验手册). Shanghai: Shanghai Science and Technology Press, pp 111-115 (in Chinese)

[20]Chen Y-W(陈一舞), Shao G-H(邵桂花), Chang R-Z(常汝镇). The effect of salt stress on superoxide dismutase in various organelles from cotyledon of soybean seedling. Acta Agron Sin (作物学报), 1997, 23(2): 214-219 (in Chinese with English abstract)

[21]Zhang Y-S(张以顺), Huang X(黄霞), Chen Y-F(陈云凤). Plant Physiology Experiments Tutorial(植物生理学实验教程), Beijing: Higher Education Press, 2009. pp 139-140 (in Chinese)

[22]Liang Y(梁颖), Wang S-G(王三根). The protective function of Ca2+ on the membrane of rice seedling under low temperature stress. Acta Agron Sin (作物学报), 2001, 27(1): 59-63 (in Chinese with English abstract)

[23]Luo Y(罗娅), Tang H-R(汤浩茹), Zhang Y(张勇). Effect of low temperature stress on activities of SOD and enzymes of ascorbate-glutathione cycle. Acta Hort Sin (园艺学报), 2007, 34(6): 1405-1410 (in Chinese with English abstract)

[24]Ren H-M(任汇淼), Wei J-M(魏家绵), Shen Y-G(沈允钢). Progress in the study on structure, function and regulation of chloroplast ATP synthase. Plant Physiol Commun (植物生理学通讯), 1994, 30(3): 161-169 (in Chinese with English abstract)

[25]Zhang J-H(张金红), Wu J-P(吴隽平), Wei J-H(魏继中), Zai Z-Q(翟治清), Zhang S-P(张遂坡), Xu Y-H(徐友涵). The effect of Pr3+, Nd3+ on activity of Ca2+, Mg2+-ATPase on plasma membrane of Vicia feba leaves. Chin J Biochem, 1992, 8(2): 195-200 (in Chinese with English abstract)

[26]Li S(李杉), Xing G-M(邢更妹), Cui K-R(崔凯荣), Yu C-H(余春红), Zhang X(张霞), Xu H-X(徐海霞), Wang Y-F(王亚馥). Ultracytochemical localization of calcium and ATPase activity on the 2,4-D induced somatic embryogenesis of Lycium barbarum L. Acta Biol Exp Sin (实验生物学报), 2003, 36(6): 414-420 (in Chinese with English abstract)

[27]Zhang J-H(张俊环), Zhang G-Q(张国强), Liu Y-P(刘悦萍), Huang W-D(黄卫东). Cytochemical localization and changes in activity of plasma membrane Ca2+-ATPase in young grape (Vitis vinifera L. cv. Jingxiu) plants during cross adaptation to temperature stresses. Sci Agric Sin (中国农业科学), 2006, 39(8): 1617-1625 (in Chinese with English abstract)

[28]Li S-L(李素丽), Yang L-T(杨丽涛), Li Z-G(李志刚), Li Y-R(李杨瑞), Han C-W(韩春旺), Liang Z-Z(梁兆宙). Response of Ca2+ and Ca2+-ATPase activity in the stem tip of sugarcane to low temperature stress. J China Agric Univ (中国农业大学学报), 2011, 16(2): 14-21 (in Chinese with English abstract)

[29]Kreash H A, Wies P R. The ultrastructure of chilling stress. Plant Cell Environ, 2000, 23: 337-350

[30]Ai X-Z(艾希珍), Wang X-F(王秀峰), Guo Y-K(郭延奎), Xing Y-X(邢禹贤). Effects of suboptimal temperature and low temperature under low light intensity on stomatal characteristics and chloroplast ultrastructure of cucumber seedlings. Sci Agri Sin (中国农业科学), 2006, 39(10): 2063-2068 (in Chinese with English abstract)

[31]Hendrickson L, Furbank R T, Chow W S. A simple alternative approach to assessing the fate of absorbed light energy using chlorophyll fluorescence. Photosynth Res, 2004, 82: 73-81

[32]Zhou Y-H(周艳虹), Huang L-F(黄黎锋), Yu J-Q(喻景权). Effects of sustained chilling and low light on gas exchange, chlorophyll fluorescence quenching and absorbed light allocation in cucumber leaves. J Plant Physiol Mol Biol (植物生理与分子生物学学报), 2004, 30(2): 153-160 (in Chinese with English abstract)

[33]Aro E, Virgin I, Anderson B. Photoinhibition of photosystem II. Inactivation, protein damage and turnover. Biochimica et Biophysica Acta, 1993, 1143: 113-134

[34]Kaniuga Z, Zabek J, Sochanowicz B. Contribution of loosely bound manganese to the mechanism of reversible in activation of hill reaction activity following cold and dark storage and illumination of leaves. Planta, 1978, 144: 49-56

[35]Kee S C, Martin B, Ort D R. The effect s of chilling in the dark and in the light on photosynthesis of tomato: electron transfer reaction. Photosynth Res, 1986, 8: 41-51

[36]Peng C-L(彭长连), Lin Z-F(林植芳), Lin G-Z(林桂珠). Superoxide production rate and photosynthetic feature in leaves of some plant species under photooxidation. Acta Phytophysiol Sin (植物生理学报), 2000, 26(2): 81-87 (in Chinese with English abstract)

[37]Fu Q-S(付秋实), LI H-L(李红岭), Cui J(崔健), Zhao B(赵冰), Guo Y-D(郭仰东). Effects of water stress on photosynthesis and associated physiological characters of Capsicum annuum L. Sci Agri Sin (中国农业科学), 2009, 42(5): 1859-1866 (in Chinese with English abstract)

[38]Wang B-L(汪炳良), Xu M(徐敏), Shi Q-H(史庆华), Cao J-S(曹家树). Effects of high temperature stress on antioxidant systems, chlorophyll and chlorophyll fluorescence parameters in early cauliflower leaves. Sci Agric Sin (中国农业科学), 2004, 37(8): 1245-1250 (in Chinese with English abstract)

[39]Qian Q-Q(钱琼秋), Zai W-S(宰文珊), Zhu Z-J(朱祝军), Yu J-Q(喻景权). Effects of exogenous silicon on active oxygen scavenging system in chloroplasts of cucumber (Cucumis sativus L.) seedlings under salt stress. J Plant Physiol Mol Biol (植物生理与分子生物学学报), 2006, 32(1): 107-112 (in Chinese with English abstract)

[40]Li J(李晶), Yan X-F(阎秀峰), Zu Y-G(祖元刚). Generation of activated oxygen and change of sell defense enzyme activity in leaves of Korean pine seedling under low temperature. Acta Bot Sin (植物学报), 2000, 42(2): 148-152 (in Chinese with English abstract)
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