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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (10): 1791-1801.doi: 10.3724/SP.J.1006.2012.01791

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Development of EST Markers Specific to Agropyron cristatum Chromosome 6P in Common Wheat Background

DAI Cheng,ZHANG Jin-Peng*, WU Xiao-Yang,YANG Xin-Ming,LI Xiu-Quan,LIU Wei-Hua,GAO Ai-Nong*,LI Li-Hui   

  1. National Key Facility for Crop Gene Resources and Genetic Improvement / Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2012-04-13 Revised:2012-06-06 Online:2012-10-12 Published:2012-07-03
  • Contact: 高爱农, E-mail: gaoainong@caas.net.cn, Tel: 010-62176077; 张锦鹏, E-mail: zhangjp@caas.net.cn, Tel: 010-62152938

Abstract:

Wheat-Agropyron cristatum (L.) Gaertn. 6P addition line has the characteristics of superior numbers of florets and kernels per spike, which can be used in the improvement of wheat varieties. In this study, we developed EST markers specific to the A. cristatum 6P chromosome using the wheat-A. cristatum 6P disomic addition line 4844-12 (2n=44) and its common wheat parent “Fukuhokomugi” as well as A. cristatum accession Z559 (2n=4x=28, PPPP). PCR primers were designed according to the sequences of ESTs gained by the transcriptome sequencing of A. cristatum. A total of 130 6P-specific molecular markers were developed from 1 453 PCR primers. These specific markers were matched with protein database from NCBI and wheat EST sequence to understand the potential function genes on chromosome 6P and the gene synteny between A. cristatum and common wheat. The results showed that functions of four A. cristatum EST sequences are related to disease and stress resistance. Thirty six A. cristatum EST sequences could be matched with the located wheat EST bin map, in which 33 (91.67%) sequences were located in the sixth homoeologous group. To validate the specificity of these markers, we detected four EST specific markers with functional annotation under different backgrounds of other seven wheat varieties. Five specific markers were selected randomly to detect wheat-A. cristatum 6P translocation lines. The results showed that these specific markers can be used to detect chromosome 6P of A. cristatum in common wheat background, and to bulky select 6P addition lines derived from common wheat and A. cristatum.

Key words: Wheat, Agropyron cristatum, P genome, Wheat-A. cristatum 6P addition line, EST specific molecular markers

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