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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (01): 12-20.doi: 10.3724/SP.J.1006.2013.00012

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

QTL Mapping of Pubescence Density and Length on Leaf Surface of Soybean

XING Guang-Nan,LIU Ze-Xi-Nan,TAN Lian-Mei,YUE Han,WANG Yu-Feng,KIM Hyun-Jee,ZHAO Tuan-Jie,GAI Jun-Yi*   

  1. Soybean Research Institute / National Center for Soybean Improvement / Key Laboratory for Biology and Genetic Improvement of Soybean (General), Ministry of Agriculture / National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2012-10-09 Revised:2012-11-14 Online:2013-01-12 Published:2012-11-14
  • Contact: 盖钧镒, E-mail: sri@njau.edu.cn, Tel: 025-84395405

Abstract:

Soybean pubescences are known to play important roles in resistance to pests and tolerance to drought stress. QTL mapping of leaf pubescence density and length was conducted in recombinant inbred line populations of NJRIKY (KY) and NJRIXG (XG). The results obtained were as follows: (1) There existed great variation and certain transgressive segregation in leaf pubescence density and length among lines; highly significant negative correlations (r= −0.49 and −0.62, respectively) between the two traits were observed; the heritability values for pubescence density ranged from 75.7% to 76.8%, higher than that for pubescence length ranged from 45.2% to 62.9% in the two populations. (2) Two major QTL for pubescence density detected were PD1-1 accounted for 20.7% of phenotypic variation in XG, and PD12-1 contributed 21.7% of phenotypic variation in KY. The genetic constitution of pubescence density was composed of additive QTL (20.7−36.2% of phenotypic variation), epistatic QTL pairs (0−1.4%) and collective unmapped minor QTL (38.1−56.1%) in the two populations. Here the unmapped minor QTL was the most important part for the trait, which was not recognized if only using mapping procedures without the consideration of the total genetic variation among the lines. (3) The phenotypic variation of pubescence length in KY was accounted for by epistatic QTL pairs (4.2%) and collective unmapped minor QTL (58.7%) without additive QTL (0%), while that in XG mainly by additive QTL, including Pl1-1 and Pl12-1 on chromosomes 1 and 12 accounting for 18.3% and 22.5% of phenotypic variation, respectively, with very small contribution by epistatic QTL pair and collective unmapped minor QTL. Therefore, the genetic constitutions of pubescence length in the two populations were different from each other. The genetic mechanisms of leaf pubescence density and length in soybean are complicated and involve many genes/QTL with different effects.

Key words: Soybean [Glycine max (L.) Merr.], Pubescence density, Pubescence length, QTL mapping

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