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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (11): 2023-2029.doi: 10.3724/SP.J.1006.2013.02023

• TILLAGE & CULTIVATION · PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Responses of Antioxidant Enzyme and PSII Electron Transport in Leaf of Transgenic Tobacco Carrying 2-Cys Prx to Salt and Light Stresses

ZHANG Hui-Hui1,TIAN Qi1,LIU Guan-Jun2,HU Yan-Bo1,WU Xiang-Yu2,TIANA Ye1,LI Xin1,SUN Guang-Yu1,*   

  1. 1 College of Life Science, Northeast Forestry University China, Harbin 150040, China; 2 State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University China, Harbin 150040, China?
  • Received:2012-10-26 Revised:2013-06-09 Online:2013-11-12 Published:2013-08-12
  • Contact: 孙广玉, E-mail:sungy@vip.sina.com

Abstract:

2-Cys peroxiredoxins (2-Cys Prxs) in plants were reported to localize to plant chloroplasts and perform antioxidative function during plant development and photosynthesis. This paper investigated the function of tobacco 2-Cys Prx gene overexpressed in transgenic tobacco (Nicotiana tabacum) plants with tolerance against light intensity and salt stresses. The results showed that superoxide dismutase (SOD) activity in leaves of two types of tobacco increased under weak light (200 μmol m–2 s–1) and salt stresses, ascorbateperoxidase (APX) activity changed little, and the content of H2O2 slightly increased. Salt stress enhanced SOD activity and decreased APX activity under strong light (1000 μmol m–2 s–1). The content of H2O2 in leaves of transgenic tobacco with 2-Cys Prx increased slower than that of wild tobacco. The electron transport rate (ETR), PSII photochemical efficiency (Fv/Fm) and actual photochemical efficiency (ФPSII) in leaves of transgenic tobacco with 2-Cys Prx were higher than those of control under high light. Electron transports in the receptor side were lower than that in control. The results suggested that 2-Cys Prx could be the effective removal of excess H2O2 while APX activity decreasedunder high light and salt stresses, and could reduce PSII photoinhibition induced by high light and salt stresses.

Key words: Transgenic tobacco carrying 2-Cys Prx, Salt and light stress, Antioxidant enzyme, PSII electron transport

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