Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (06): 1125-1131.doi: 10.3724/SP.J.1006.2014.01125

• RESEARCH NOTES • Previous Articles     Next Articles

Screening of a New Chemical Hybridizing Agent in Wheat

SONG Yu-Long,ZHANG Peng-Fei,ZHANG Gai-Sheng*,ZHAO Zhuo-Jun,NIU Na,ZHAO Xin-Liang,WANG Jun-Wei   

  1. College of Agronomy, Northwest A&F University/ National Yangling Agricultural Biotechnology & Breeding Center / Yangling Branch of State Wheat Improvement Centre /Wheat Breeding Engineering Research Center of Ministry of Education / Key Laboratory of Crop Heterosis of Shaanxi Province, Yangling 712100, China
  • Received:2013-11-06 Revised:2014-03-04 Online:2014-06-12 Published:2014-03-25

Abstract:

To expand varieties of chemical hybridization agent and reduce their cost, We selected 15 varieties of chemical hybridization agent for treating wheat cultivar Xi’nong 1376 at three growth stages with four concentrations to survey the infertile effect, phytotoxicity and pollen abortion mechanism. The results showed that there were significant differences among the chemical hybridization agent treatments. At stages Feek’s 7.5 and Feek’s 9.5, abnormal vegetative growth or pollens abortion were not obvious. While at the stage of Feek’s 8.5, the rate of male sterility of Xinong 1376 was 93.33% in 0.24 kg ha-1 T6 treatment, and the pistil and the plant showed normal development, we also achieved a high seed setting rate via crossing the male sterile plants with normal fertile lines by saturate pollination. Paraffin sections indicated that the degradable process of tapetum cells in the male sterile anther treated by T6-C was abnormal. The nucleus of tapetum cells degraded at uninucleate early stage, and then pollen nucleus degraded at uninucleate late stage. Tapetum cells, nucleus of pollen and nutrients were almost disappeared till trinucleate stage. Finally, a little residue of pollen wall left and pollen aborted. Therefore, we hypothesize that the male sterility induced by T6 could be related to the degradation of tapetum cells in anther.

Key words: Wheat, Chemical hybrid agents, Saturate pollination, Sterility ratio, Tapetum

[1]金双义, 范军. 对小麦杂种优势研究方法的探讨. 中国新技术新产品, 2008, 12(上): 156



Jin S Y, Fan J. Study on heterosis of research methods in wheat. China New Technol Prod, 2008, 12(scroller): 156 (in Chinese)



[2]辛金霞, 戎郁萍. 化学杂交剂在植物育种中的应用现状. 草业科学, 2010, 27(3): 124–131



Xin J X, Rong Y P. Application status of chemical hybridizing agent in plant breeding. Pratacul Tural Sci, 2010, 27(3): 124–131 (in Chinese)



[3]魏正平, 刘树人, 翟玉洁, 王岩, 于天峰, 刁艳玲. 化学杂交剂BAU-2诱导春小麦雄性不育的研究. 北京农业大学学报, 1993, 19(增刊): 25–29



Wei Z P, Liu S R, Zhai Y J, Wang Y, Yu T F, Diao Y L. Studies on the male sterility induced by CHA-BAU-2 in spring wheat. Acta Agric Univ Pekinensis, 1993, 19(suppl): 25–29 (in Chinese with English abstract)



[4]黄铁城, 王明理, 张爱民, 张仲琦. 新型化学杂交剂WL84811诱导普通小麦(Triticum aestivum)雄性不育的研究. 作物学报, 1988, 14(2): 155–162



Huang T C, Wang M L, Zhang A M, Zhang Z Q. Studies on the male sterility in wheat (Triticum aestivum) induced by new gametocide WL84811. Acta Agron Sin, 1988, 14(2): 155–162 (in Chinese with English abstract)



[5]梁肖青. SC2053诱导小麦雄性不育的效果研究. 山西农业大学学报, 2006, 5(5): 92–94



Liang X Q. Analysis on the male sterility in wheat induced by SC2053. J Shanxi Agric Univ, 2006, 5(5): 92–94 (in Chinese with English abstract)



[6]高庆荣, 刘保申, 于金凤, 孙兰珍. 化学杂交剂GENESIS对小麦的杀雄效果. 山东农业大学学报(自然科学版), 2002, 33(1): 1–6



Gao Q R, Liu B S, Yu J F, Sun L Z. The Effect of CHA-GENESISI on growth and development of winter wheat. J Shandong Agric Univ (Nat Sci Edn), 2002, 33(1): 1–6 (in Chinese with English abstract)



[7]王振华, 刘宏伟, 张改生, 王军卫, 马守才. 3种新型化学杂交剂诱导小麦雄性不育效果比较. 西北农林科技大学(自然科学版), 2003, 31(3): 43–46



Wang Z H, Liu H W, Zhang G S, Wang J W, Ma S C. Effects on male sterility induced by three kinds of chemical hybridization agent in wheat. J Northwest Sci Tech Univ Agric For (Nat Sci Edn), 2003, 31(3): 43–46 (in Chinese with English abstract)



[8]武计萍, 胡金锁, 李国强, 仇松英, 逯腊虎, 孟兆萍. 化学杂交剂SQ-1诱导小麦雄性不育及与不同小麦品种互作效应的研究. 小麦研究, 2006, 27(3): 12–15



Wu J P, Hu J S, Li G Q, Qiu S Y, Lu L H, Meng Z P. Effect of male sterility on different wheat genotype induced by SQ-1. Wheat Res, 2006, 27(3): 12–15 (in Chinese with English abstract)



[9]叶景秀, 陈蕊红, 张改生, 王俊生, 王书平, 李莉, 牛娜, 马守才, 李红霞, 朱建楚.杀雄剂CULT诱导小麦雄性不育花药蛋白质组分分析. 农业生物技术学报, 2009, 17: 858–864



Ye J X, Chen R H, Zhang G S, Wang J S, Wang S P, Li L, Niu N, Ma S C, Li H X, Zhu J C. Analysis on anther proteins of wheat male sterile line induced by chemical hybridizing agent SQ-1. J Agric Biotechnol, 2009, 17: 858?864 (in Chinese with English abstract)



[10]宋瑜龙, 王亮明, 张改生, 盛英, 李亚鑫, 牛娜, 赵卓军. 杀雄剂SQ-1诱导谷子雄性不育研究. 作物学报, 2011, 37: 1695–1700



Song Y L, Wang L M, Zhang G S, Sheng Y, Li Y X, Niu N, Zhao Z J. Male sterility induced by chemical hybridizing agent SQ-1 in Setaria italica Beauv. Acta Agron Sin, 2011, 37: 1695–1700 (in Chinese with English abstract)



[11]盛英, 张改生, 李亚鑫, 张龙雨, 王书平, 赵新亮, 王亮明, 宋瑜龙. 小麦生理型雄性不育花药绒毡层和孢粉素变化与 RAFTIN1表达的关系. 中国农业科学, 2011, 44: 3937–3944



Sheng Y, Zhang G S, Li Y X, Zhang L Y, Wang S P, Zhao X L, Wang L M, Song Y L. The relationship on anther tapetum, sporopollenin and expression of RAFTIN1 in physiological male sterile wheat. Sci Agric Sin, 2011, 44: 3937–3944 (in Chinese with English abstract)



[12]尹梦回, 董静, 李先碧, 侯磊, 罗明, 李德谋, 裴炎, 肖月华. 烟草绒毡层特异启动子pTA29在棉花中的表达特性. 作物学报, 2008, 34(12): 2092–2098



Yin M H, Dong J, Li X B, Hou L, Luo M, Li D M, Pei Y, Xiao Y H. Expression characteristics of tobacco tapetum-specific promoter pTA29 in cotton. Acta Agron Sin, 2008, 34: 2092–2098 (in Chinese with English abstract)



[13]Li Y Y, Yang Q, Yan J Y, Chen M. Cytoplasmic male-sterility of onion (Allium cepa L.) 63A is associated with premature degeneration of the tapetum. Acta Agron Sin, 2006, 32(03): 369–372



[14]苏少泉. 草甘膦述评. 农药, 2005, 44(4): 145–149



Su S Q. Glyphosate review. Chin J Pesticides, 2005, 44(4): 145–149 (in Chinese with English abstract)



[15]许海霞, 吕德彬, 程西永, 詹克慧, 陈军营, 姜鸿勋. GENESIS诱导小麦雄性不育的形态学和细胞学观察. 河南农业大学学报, 2003, 9: 205–208



Xu H X, Lü D B, Cheng X Y, Zhan K H, Chen J Y, Jiang H X. Morphological and cytological observations of male sterility induced by CHA GENESIS in wheat. J Henan Agric Univ, 2003, 9: 205–208 (in Chinese with English abstract)



[16]徐如强, 黄铁城. “BAU-2”诱导普通小麦雄性不育的研究: III. 化学杀雄机制. 北京农业大学学报, 1993, 19(增刊): 19–24



Xu R Q, Huang T C. Studies on BAU-2 induced male sterility in winter wheat (T. aestivum): III. The methanism of BAU-2 inducing male sterility. Acta Agric Univ Pekinesis, 1993, 19(suppl): 19–24 (in Chinese with English abstract)



[17]范平, 崔党群, 范弘伟. CHA-SC2053诱导普通小麦雄性不育研究. 河南农业大学学报, 1998, 32: 149–153



Fan P, Cui D Q, Fan H W. Studies on the male sterility induced by CHA-SC2053 in common wheat. Acta Agric Univ Henanensis, 1998, 32: 149–153 (in Chinese with English abstract)



[18]杨武云, 饶世达. 小麦新型化杀剂SC2053诱导花粉败育的机制研究. 北京农业大学学报, 1993, 19(增刊): 88–91



Yang W Y, Rao S D. Cytological study on the new type chemical hybridizing agent SC2053 inducing wheat pollen abortion. Acta Agric Univ Pekinesis, 1993, 19(suppl): 88–91 (in Chinese with English abstract)

[1] Mao Jia-Qi, Huang Peng-Yu, Zhao Jia-Jia, Zheng Xing-Wei, Wu Bang-Bang, Hao Yu-Qiong, Qu Fei, Liu Cheng, Ma Peng-Tao, Zheng Jun. Evaluation of powdery mildew resistance in wheat cultivars and molecular detection of resistance genes in Shanxi province, China [J]. Acta Agronomica Sinica, 2026, 52(6): 1669-1681.
[2] Hu Chuan, Zhao Kai-Nan, Huang Xiu-Li, Wu Jin-Zhi, Ren Kai-Ming, Wang He-Zheng, Fu Guo-Zhan, Huang Ming, Li You-Jun. Effects of tillage methods and nitrogen rates on yield and quality of dryland wheat under one-off irrigation [J]. Acta Agronomica Sinica, 2026, 52(6): 1830-1846.
[3] Chen Xue-Yan, He Hua-Chuan, Li Zheng-Jia, Dong Xin-Pan, Li Ou-Qi, Liu Xiao-Yun, Li Dan-Ping, Chen Zhi-Wei, Liu Guo-Xia, Lyu Sheng-Yuan, Wu Yin-Ying, Zhao Zhen-Dong, Cao Xin-You, Wan He-Ping. Dynamic changes in root organic acid secretion and its transcriptional regulatory mechanisms in ‘Jimai 60’ seedlings under combined salinity-alkalinity stress in hydroponics [J]. Acta Agronomica Sinica, 2026, 52(6): 1859-1875.
[4] Gao Pei-Yang, Li Jin-Xuan, Dong Yu-Kui, Shi Yu, Zhang Zhen, Zhang Yong-Li. Response of wheat tillering and spike formation to nitrogen rate under supplementary irrigation based on soil moisture content [J]. Acta Agronomica Sinica, 2026, 52(6): 1847-1858.
[5] Zhang Xian-Feng, Guo Li-Jian, Li Kang-Chun, Kong Bin-Xue, Liu Yu-Fang, Che Zhuo, Yang De-Long. Identification of the ABHD6 gene family and development of functional markers for grain weight in wheat [J]. Acta Agronomica Sinica, 2026, 52(6): 1711-1727.
[6] Zhai Sheng-Nan, Cao Xin-You, Li Hao-Sheng, Li Ji-Hu, Li Fa-Ji, Liu Jin-Dong, Xia Xian-Chun, Lyu Ying-Ying, Ma Rui-Feng, Wang Ying, Geng Hong-Wei, Liu Jian-Jun. Analysis of the genetic effects of allelic variation at the Pod-A1, Pod-D1, and Pod-2D loci on peroxidase activity in wheat grains [J]. Acta Agronomica Sinica, 2026, 52(6): 1593-1603.
[7] Xi Qian-Hui, Xu Zi-Yuan, Liu Meng-Meng, Wang Hong-Yi, Lang Kai-Lin, Jing Zhen-Hai, Chen Feng, Zhao Lei. Genome-wide association study and candidate gene prediction of grain copper content in wheat [J]. Acta Agronomica Sinica, 2026, 52(6): 1604-1617.
[8] Wang Zhuang-Zhuang, Wu Zi-Jun, Zhang Yong-Xin, Zhang Xin-Yuan, Yuan Li-Xue, Chen Ru-Xue, Liu Shi-Ju, Duan Jian-Zhao, Feng Wei, Wang Tong-Chao, Wang Yong-Hua. Optimized water-nitrogen synergy enhances winter wheat yield and nitrogen use efficiency in clay-loam fluvo-aquic soils of southeastern Henan, China [J]. Acta Agronomica Sinica, 2026, 52(5): 1501-1521.
[9] He Wan-Long, Geng Hong-Wei, Zhang Fei-Fei, Mikereayi·Ababaikere , Luo Zi-Yang, Li Peng-Cheng, Zhou Zhao-Yu, Cheng Yu-Kun. Development of a deep learning-based image recognition system for major wheat diseases [J]. Acta Agronomica Sinica, 2026, 52(5): 1401-1417.
[10] Zhang Zhen, Feng Lian-Jie, Shi Yu, Yu Zhen-Wen, Zhang Yong-Li. Yield formation of wheat with different ear types under water-saving supplementary irrigation conditions [J]. Acta Agronomica Sinica, 2026, 52(5): 1522-1535.
[11] Hou Si-Yu, Wang Guo-Cui, Wei Jin-Gui, Xie Wei-Xin, Yin Wen, Fan Zhi-Long, Chai Qiang, Hu Fa-Long. Effects of green manure combined with chemical nitrogen fertilizer on dry matter accumulation and yield formation of wheat in arid irrigation areas of northwestern China [J]. Acta Agronomica Sinica, 2026, 52(4): 1208-1219.
[12] Shang Yun-Qiu, Zhao Zhu, Chen Huan, Ding Yong-Gang, Qiao Yu-Qiang, Li Wei, Zhang Xiang-Qian, Cao Cheng-Fu, Du Shi-Zhou. Effects of long-term tillage practices on grain-filling and yield formation in rain-fed wheat [J]. Acta Agronomica Sinica, 2026, 52(4): 1236-1250.
[13] Qiao Yu-Xin, Li Cheng-Yue, Kang Xiao-Yu, Zhang Xin-Qi, Jia Shao-Hui, Liu Qian, Cao Ya-Li, Shi Xin-Rui, Hao Xing-Yu, Li Ping. Study on the effects of long-term no-tillage straw mulching on wheat yield improvement in dryland areas based on the APSIM model [J]. Acta Agronomica Sinica, 2026, 52(4): 1181-1192.
[14] Li Can, Zhang Xi-Wei, Zhu Bo-Tao, Zhang Pei-Pei. Functional characterization of wheat GSK kinase TaSK41 and screening for interacting proteins [J]. Acta Agronomica Sinica, 2026, 52(3): 677-687.
[15] Hou Jie, Fu Duo-Duo, Wu Hai-Feng, Hao Yu-Qiong, Zheng Xing-Wei, Wu Bang-Bang, Zhou Kai, Li Xiao-Hua, Zheng Jun, Zhao Jia-Jia. Chromosome diversity and its effects in wheat landraces from Shanxi province, China [J]. Acta Agronomica Sinica, 2026, 52(3): 746-763.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!