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Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (08): 1350-1355.doi: 10.3724/SP.J.1006.2014.01350

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

GhCPS and GhKS Encoding Gibberellin Biosynthesis Enzymes Involve in Inhibition of Leaf Growth by Mepiquat Chloride in Cotton (Gossypium hirsutum L.)

WANG Li1,2,ZHANG Ming-Cai1,DU Ming-Wei1,TIAN Xiao-Li1,LI Zhao-Hu1,*   

  1. 1 Engineering Research Center of Plant Growth Regulator, Ministry of Education / College of Agronomy and Biotechnology, China Agricultural University, Beijing 10093, China; 2 College of Life Science, Henan Normal University, Xinxiang 453007, China
  • Received:2014-03-06 Revised:2014-04-16 Online:2014-08-12 Published:2014-06-03
  • Contact: 李召虎, E-mail: lizhaohu@cau.edu.cn, Tel: 010-62733427

Abstract:

Ent-copalyl diphosphate synthase (CPS) and ent-kaurene synthase (KS) are the key enzymes involved in the early steps of gibberellin (GA) biosynthesis. This paper aimed at elucidating whether the action of mepiquat chloride (DPC) on leaf growth was related to the expression levels of GhCPS and GhKS in cotton seedlings. DPC was foliar applied to seedlings at the 3rd leaf expanded stage of cotton cultivar Xinkang 4 by pot culture. The results showed that DPC significantly decreased the leaf area, and the area of the 4th leaf was decreased more than that of the 3rd leaf. DPC at 80 mg L–1 markedly reduced GA4 content in the 3rd leaf at four days after treatment and in the 4th leaf from four to six days after treatment. The expression levels of GhCPS and GhKS in the 3rd leaf were decreased by DPC from one to four days after treatment, and similar trends were observed in the 4th leaf from one to six days after treatment. All the results suggested that DPC could reduce endogenous GA4 content by downregulating GhCPS and GhKS expressions, leading to a smaller leaf size. Otherwise, the younger leaf was more sensitive to DPC.

Key words: Cotton, Mepiquat chloride, Ent-copalyl diphosphate synthase, Ent-kaurene synthase;Leaf area

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