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Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (11): 1946-1955.doi: 10.3724/SP.J.1006.2014.01946

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Physiological Characteristics and Gene Mapping of a Leaf Early-Senescence Mutant osled in Rice

ZHAO Chen-Chen1,HUANG Fu-Deng2,GONG Pan1,YANG Xi1,CHENG Fang-Min1,PAN Gang1,*   

  1. 1 College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China; 2 Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
  • Received:2014-03-21 Revised:2014-09-16 Online:2014-11-12 Published:2014-09-26
  • Contact: 潘刚, E-mail: pangang12@126.com

Abstract:

Leaf early senescence directly affects crop yield and quality, therefore, studying the molecular and physiological mechanism of leaf early senescence means a lot to crop genetic improvement. This work used 60Co γ-radiation to radiate rice Varity 93-11 and a leaf early senescence mutant osled was obtained. The mutant senesced quite early even at tillering stage, showing grey at the tip and edge of leaves and red-brown lesion. Under the treatment of simulative drought stress, the mutant senesced at early seedlings and its plant height and root length were obviously shortened. Analysis in physiology and biochemistry showed the content of malondialdehyde (MDA), activities of superoxide dismutase (SOD) and peroxidase (POD) were basically unchanged from flag leaf to third-top leaf in wild type, while significantly increased, and dramatically higher in the second-top and third-top leaves of mutant. Contents of soluble protein and chlorophyll, and catalase (CAT) activity all gradually reduced in top three leaves of both the mutant and wild type, however, contents in second-top and third-top leaves of the mutant were quite lower than these in wild type. Histochemical stainings of trypan blue, 3,3’-diaminobenzidine (DAB), and nitro blue tetrazolium (NBT) not only suggested the destructions of membrane system but also illustrated the accumulation of H2O2 and O2?  in the mutant. Besides, ultrastructure of mesophyll cells further explained the degradation of chloroplast in osled. Genetic analysis suggested the osled was controlled by a single recessive gene. Through using gene mapping technologies, the OsLED gene was successfully mapped into an interval from marker RM15528 to marker RM15553 at the long arm of chromosome 3.Genetic distances were both 0.7 cM. These results laid a foundation for later gene cloning and its functions study.

Key words: Rice, Leaf early senescence, Simulated drought stress, Physiological analysis, Gene mapping

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