Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2015, Vol. 41 ›› Issue (01): 22-30.doi: 10.3724/SP.J.1006.2015.00022


Effects of Glu-1 Deletion on Size Distribution of Glutenin Polymeric Protein and Dough Properties in Common Wheat

ZHANG Ping-Ping,MA Hong-Xiang,YAO Jin-Bao,Joseph M. AWIKA   

  1. 1 Provincial Key Laboratory for Agrobiology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China; 2Cereal Quality Laboratory, Department of Soil and Crop Sciences, Texas A&M University, College Station, TX 77843, USA
  • Received:2014-03-24 Revised:2014-09-30 Online:2015-01-12 Published:2014-11-11
  • Contact: 张平平, E-mail: pp_zh@126.com, Tel: 025-84390257


The relationship between the HMW-GS and LMW-GS components and the size distribution of glutenin polymeric protein and dough properties were studied using Glu-1 normal and deletion lines, which provides a foundation for investigating the potential of Glu-1 deletion line in quality improvement of winter wheat. In the 20 hard white winter wheat lines tested, one was Glu-A1 deletion line, five were Glu-D1 deletion lines, and three were Glu-A1 and Glu-D1 double-deletion lines. Flour protein contents of the lines tested ranged from 13.39% to 14.12% with no significant difference between each other and between deletion and non-deletion groups. In Glu-1 deletion lines, the high molecular weight glutenin subunits/low molecular weight glutenin subunits ratio (HMW/LMW), content of SDS unextractable polymeric protein (UPP), and percentage of UPP (%UPP) were significantly lower than those in non-deletion lines. However, the glutenin/gliadin ratio (GLU/GLI) was similar in all genotypes, with no significant difference between deletion and non-deletion groups. Deletion at Glu-1 resulted in a significant decrease of dough elasticity and a significant increase of dough extensibility. Several Glu-1 deletion lines were characterized with medium gluten strength and excellent extensibility, suggesting that the size distribution of glutenin polymeric protein and dough properties were affected by both allelic composition and quantity of glutenin subunits. These results indicate that Glu-1 deletion lines can be used to improve dough extensibility and processing quality of common wheat.

Key words: Glu-1 locus, Deletion, Glutenin polymeric protein, Dough properties

[1]Payne P I, Nightingale M A, Krattiger A F, Holt L M. The relationship between HMW glutenin subunit composition and the bread-making quality of British-grown wheat varieties. J Sci Food Agric, 1987, 40: 51–65

[2]赵和, 卢少源, 李宗智. 小麦高分子量麦谷蛋白遗传变异及其与品质和其它农艺性状关系的研究. 作物学报, 1994, 20: 67–75

Zhao H, Lu S Y, Li Z Z. Studies on inheritance and variation of HMW glutenin subunits and their correlation with quality and other agronomic characters in wheat. Acta Agron Sin, 1994, 20: 67–75

[3]宋建民, 吴祥云, 刘建军, 刘爱峰, 赵振东, 刘广田. 小麦品质的谷蛋白亚基评定标准研究. 作物学报, 2003, 29: 829–834

Song J M, Wu X Y, Liu J J, Liu A F, Zhao Z D, Liu G T. Study on quality scoring system assessed by wheat high-molecular-weight glutenin subunits. Acta Agron Sin, 2003, 29: 829–834 (in Chinese with English abstract)

[4]庞斌双, 张学勇, 王兰芬, 郝晨阳, 董玉琛. 小麦Glu-B1位点1Bx14+1By18新亚基对材料的创制及其对加工质量的影响分析. 作物学报, 2007, 33:1582–1586

Pang B S, Zang X Y, Wang L F, Hao C Y, Dong Y C. A novel line with HMW-GS 1Bx14+1By18 with positive effect on processing quality. Acta Agron Sin, 2007, 33: 1582–1586 (in Chinese with English abstract)

[5]张莉丽, 张延滨, 宋庆杰, 赵海滨, 于海洋, 张春利, 辛文利, 肖志敏. 龙辐麦3号小麦品种HMW-GS NULL和1近等基因系间品质差异的研究. 中国农业科学, 2007, 40: 1864–1870

Zhang L L, Zhang Y B, Song Q J, Zhao H B, Yu H Y, Zhang C L, Xin W L, Xiao Z M. Study on the quality of NILs of wheat cultivar Longfumai 3 with HMW-GS null and 1 subunits. Sci Agric Sin, 2007, 40: 1864–1870 (in Chinese with English abstract)

[6]León E, Aouni R, Piston F, Rodríguez-Quijano M, Shewry P R, Martín A, Barro F. Stacking HMW-GS transgenes in bread wheat: Combining subunit 1Dy10 gives improved mixing properties and dough functionality. J Cereal Sci, 2010, 51: 13–20

[7]Gianibelli M C, Larroque O R, MacRitchie F. Biochemical, genetic, molecular characterization of wheat glutenin and its component subunits. Cereal Chem, 2001, 78: 635–646

[8]Rasheed A, Xia X C, Yan Y M, Appels R, Mahmoodb T. He Z H. Wheat seed storage proteins: Advances in molecular genetics, diversity and breeding applications. J Cereal Sci, 2014, 60: 11–24.

[9]Lindsay M P, Skerritt J H. The glutenin macropolymer of wheat flour dough: structure-function perspectives. Trends Foods Sci Tech, 1999, 10: 247–253

[10]Ciaffi M, Tozzi L, Lafiandra D. Relationship between flour composition determined by size-exclusion high-performance liquid chromatography and dough rheological parameters. Cereal Chem, 1996, 73: 346–351

[11]Gupta R B, Khan K, MacRitchie F. Biochemical basis of flour properties in bread wheats. I. Effects of variation in the quantity and size distribution of polymeric protein. J Cereal Sci, 1993, 18: 23-41

[12]赵会贤, 胡胜武, 吉万全, Daryl M. 麦谷蛋白Glu-1和Glu-3位点基因等位变异对籽粒聚合体蛋白粒度分布的影响. 中国农业科学, 1998, 31(1): 69–75

Zhao H X, Hu S W, Ji W Q, Daryl M. The effects of allelic variation at glutenin subunit loci Glu-1 and Glu-3 on the size distribution of polymeric protein. Sci Agric Sin, 1998, 31(1): 69–75 (in Chinese with English abstract)

[13]刘丽, 周阳, 何中虎, Peña R J, 张立平. Glu-1和Glu-3等位变异对不溶性谷蛋白含量的影响. 作物学报, 2004, 30: 1086–1092

Liu L, Zhou Y, He Z H, Peña R J, Zhang L P. Effect of allelic variation at Glu-1 and Glu-3 loci on insoluble glutenin content. Acta Agron Sin, 2004, 30: 1086–1092 (in Chinese with English abstract)

[14]Zhang P P, He Z H, Zhang Y, Xia X C, Liu J J, Yan J, Zhang Y. Pan bread and Chinese white salted noodle qualities of Chinese winter wheat cultivars and their relationship with gluten protein fractions. Cereal Chem, 2007, 84: 370–378

[15]张平平, 马鸿翔, 姚金保, 何中虎. Glu-1位点等位变异及表达量对麦谷蛋白聚合体粒度分布的影响. 作物学报, 2009, 35: 1606–1612

Zhang P P, Ma H X, Yao J B, He Z H. Effect of allelic variation and expression quantity at Glu-1 loci on size distribution of glutenin polymer in common wheat. Acta Agron Sin, 2009, 35: 1606–1612 (in Chinese with English abstract)

[16]Larroque O R, Gianibelli M C, Lafiandra D, Sharp P, Bekes F. The molecular weight distribution of the glutenin polymer as affected by the number, type and expression levels of HMW-GS. In: Pogna N E, Romanò M, Pogna E A, Galterio G, eds. Proceedings of 10th International Wheat Genetics Symposium, Poestum, Roma, Italy, 2003. pp 447–450

[17]武茹, 张晓, 高德荣, 别同德, 吴荣林, 程顺和. Glu-A1和Glu-D1位点高分子量谷蛋白亚基共同缺失对弱筋小麦品质的影响. 麦类作物学报, 2011, 31: 450–454

Wu R, Zhang X, Gao D R, Bie T D, Wu R L, Cheng S H. Effect of double deletion of Glu-A1 and Glu-D1 locus high molecular of weight glutenin subunits on wheat quality. J Triticeae Crops, 2011, 31: 450–454 (in Chinese with English abstract)

[18]Jondiko T O, Alviola N J, Hays D B, Ibrahim A, Tilley M, Awika J M. Effect of high-molecular-weight glutenin subunit allelic composition on wheat flour tortilla quality. Cereal Chem, 2012, 89: 155–161

[19]Lawrence G J. Dough and baking quality of wheat line deficient in glutenin subunits controlled by the Glu-A1, Glu-B1 and Glu-D1 loci. J Cereal Sci, 1988, 7: 109–112

[20]Zhu Y F, Li Y W, Chen Y, Li H, Liang H, Yue S J, Zhang A M, Zhang X Q, Wang D W, Jia X. Generation and characterization of a high molecular weight glutenin 1Bx14-deficient mutant in common wheat. Plant Breed, 2005, 124: 421–427

[21]刘悦, 杨足君, 李光蓉, 刘明镜, 黄健, 任正隆. 四川南部和重庆地区小麦地方品种的高分子量谷蛋白亚基组成分析. 麦类作物学报, 2007, 27: 820–824

Liu Y, Yang Z J, Li G R, Liu M J, Huang J, Ren Z L. Diversity of HMW-GS in wheat landraces native to southern sichuan and Chongqing J Triticeae Crops, 2007, 27: 820–824

[22]杨恩年, 张洁, 杨武云, 邹裕春. 六倍体普通小麦高分子量谷蛋白亚基Glu-A1和Glu-B1共同缺失材料研究初报. 西南农业学报, 2007, 20: 1293–1295

Yang E N, Zhang J, Yang W Y, Zou Y C. A common wheat line with deletion of Glu-A1 and Glu-B1 locus of high molecular weight glutenin subunit. Southwest China J Agric Sci, 2007, 20: 1293–1295

[23]AACC International, Approved Methods of Analysis, 11th Edn. Available online only. AACCI: St. Paul, MN (2010). http://methods.aaccnet.org/toc.aspx

[24]Singh N K, Shepherd K W, Cornish G B. A simplified SDS-PAGE procedure for separating LMW subunits of glutenin. J Cereal Sci, 1991, 14: 203–208

[25]Liu L, He Z H, Yan J, Zhang Y, Xia X C, Peña R J. Allelic variation at the Glu-1 and Glu-3 loci, presence of 1B•1R translocation, and their effects on mixographic properties in Chinese bread wheats. Euphytica, 2005, 142: 197–204

[26]刘丽, 周阳, 何中虎, 阎俊, 张艳, Peña R J. Glu-1和Glu-3等位变异对小麦加工品质的影响. 作物学报, 2004, 30: 959–968

Liu L, Zhou Y, He Z H, Yan J, Zhang Y, Peña R J. Efect of aIlelic variation at Glu-1 and Glu-3 loci on processing quality in common wheat. Acta Agron Sin, 2004, 30: 959–968 (in Chinese with English abstract)

[27]张平平, 张勇, 夏先春, 何中虎. 小麦贮藏蛋白反相高效液相色谱分析体系研究. 中国农业科学, 2007, 40: 1002–1009

Zhang P P, Zhang Y, Xia X C, He Z H. Protocol establishment of reversed-phase high-performance liquid chromatography (RP-HPLC) for analyzing wheat gluten protein. Sci Agric Sin, 2007, 40: 1002–1009 (in Chinese with English abstract)

[28]Larroque O R, Gianibelli M C, Gomez Sanchez M, MacRitchie F. Procedure for obtaining stable protein extracts of cereal flour and whole meal for size-exclusion HPLC analysis. Cereal Chem, 2000, 77: 448–450

[29]Payne P I. Genetics of wheat storage protein and the effect of allelic variation on bread making quality. Ann Rev Plant Physiol, 1987, 38: 141–153

[30]Vawser M J, Cornish G B. Over-expression of HMW glutenin subunit Glu-B1x7 in hexaploid wheat varieties (Triticum aestivum). Aust J Agric Res, 2004, 55: 577–588

[31]Wieser H, Zimmermann G. Importance of amounts and proportions of high molecular weight subunits of glutenin for wheat quality. Eur Food Res Tech, 2000, 210: 324–330

[32]Huang D Y, Khan K. Characterization and quantification of native glutenin aggregates by multistacking sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) procedures. Cereal Chem, 1997, 74: 229–234

[33]Zhu J, Khan K. Quantitative variation of HMW glutenin subunits from hard red spring wheats grown in different environments. Cereal Chem, 2002, 79: 783–786

[34]Branlard G. Prediction of the bread wheat quality from HMW glutenins and gliadins. In: Lasztity L, Bekes F, eds. Gluten Proteins: Proceedings of the Third Gluten Protein Workshop. Budapest, Hungar, 1987. pp 604–612

[35]Kolster P, van Eeuwijk F A, van Gelder W M J. Additive and epistatic effects of allelic variation at the high molecular weight glutenin subunit loci in determining the bread-making quality of breeding lines of wheat. Euphytica, 1991, 55: 277–285

[36]Wieser H, Kieffer R. Correlations of the amount of gluten protein types to the technological properties of wheat flours determined on a micro-scale. J Cereal Sci, 2001, 34: 19–27

[37]张平平, 姚金保, 马鸿翔, 杨学明, 张鹏, 姚国才, 张旭, 杨丹. 宁麦9号衍生系的品质特性及与酥性饼干直径的关系. 麦类作物学报, 2013, 33: 1156–1161

Zhang P P, Yao J B, Ma H X, Yang X M, Zhang P, Yao G C, Zhang X, Yang D. Quality traits of Ningmai 9 and its derivate lines and their relationship with coolie diameter. J Triticeae Crops, 2013, 33: 1156–1161 (in Chinese with English abstract)

[38]Zhang Q J, Zhang Y, Zhang Y, He Z H, Peña R J. Effects of solvent retention capacities, pentosan content, and dough rheological properties on sugar snap cookie quality in Chinese soft wheat genotypes. Crop Sci, 2007, 47: 656–664

[39]Redinbaugh M G. Soft wheat quality targets for cultivars developed for the eastern U.S. Soft Wheat Quality Laboratory Annual Report, USDA-ARS, Soft Wheat Quality Lab., Ohio Agricultural Research and Development Center, Wooster, OH, USA, 2012. pp 24–26

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