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Acta Agron Sin ›› 2015, Vol. 41 ›› Issue (02): 228-239.doi: 10.3724/SP.J.1006.2015.00228

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Expression Changes of Transcription Factors in Susceptible and Resistant Upland Cotton Cultivars (Gossypium hirsutum L.) in Response to Fusarium wilt

HAN Ze-Gang1,ZHAO Zeng-Qiang1,HE Lan-Lan1,CHAI Meng-Liang2,LI Hui-Hui1,ZHANG Wei1,*   

  1. 1 Agricultural College, Shihezi University, Shihezi 832000, China; 2 College of Life Sciences, Shihezi University, Shihezi 832000, China
  • Received:2014-05-27 Revised:2014-09-30 Online:2015-02-12 Published:2014-11-11
  • Contact: 张薇, E-mail: zhw_agr@shzu.edu.cn E-mail:hanzegang501706851@126.com

Abstract:

The Solexa sequencing was used to study the expression changes of transcription factors and their families in upland cotton cultivars susceptible and resistant to Fusarium wilt. A total of 39 transcription factor families and 433 transcription factors in Zhongmiansuo 12 and 58 transcription factor families and 588 transcription factors in Xinluzao 7 were changed in expression in at least one comparison group induced by Fusarium wilt. The number of transcription factor families responsed to Fusarium wilt was more in Xinluzao 7 than in Zhongmiansuo 12, and the number of down-regulated genes was more than that of up-regulated genes in two varieties. After the induction by Fusarium wilt, in the process of time, the number of transcription factor families and transcription factors responsed to the Fusarium wilt in two cultivars showed the same changing trend of increase first and decrease then. The maximum number was reached after six hours in Zhongmiansuo 12, while at three hours after the inducing in Xinluzao 7. Among six comparison groups, there were nine overlapping transcription factors in six transcription factor families of Zhongmiansuo 12 and Zhongmiansuo 31 overlapping transcription factors in 17 transcription factor families of Xinluzao 7. The expression of transcription factor showed strong cultivar specificity among varieties tolerant to Fusarium wilt. Except for 37 transcription factor families shared between two cultivars, two transcription factor families were unique to Zhongmiansuo 12 and Zhongmiansuo 15 transcription factor families were unique to Xinluzao 7.

Key words: Upland cotton, Fusarium wilt, Solexa sequencing, Transcription factor

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