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Acta Agron Sin ›› 2015, Vol. 41 ›› Issue (09): 1324-1332.doi: 10.3724/SP.J.1006.2015.01324

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

cDNA Cloning and Analysis of Cinnamoyl-CoA Reductase Gene from Boehmeria nivea

TANG Ying-Hong1, **, CHEN Jian-Rong2, **, LIU Fang2, YUAN You-Mei1, GUO Qing-Quan2, *, CHANG Hong-Tao2   

  1. 1 College of Agricultural, Hunan Agricultural University, Changsha 410128, China; 2 Department of Biological and Environmental Engineering, Changsha University, Changsha 410003, China
  • Received:2014-12-26 Online:2015-09-12 Published:2015-09-12

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Abstract: Two cDNA sequences of cinnamoyl-CoA reductase genes were cloned by RACE technology base on transcriptome sequencing data of Boehmeria nivea, and their bioinformatics were analyzed. Their expression levels in tissues of phloem and xylem were also tested respectively in rapid-growth stage, maturation stage and late maturity stage by quantitative Real-time PCR. The results showed that the BnCCR1 was 1056 bp in length and encoding 277 amino acids. Blast and protein structure analysis showed that its cDNA sequence shared a homology of 70% compared with Betula platyphylla and Ricinus communis CCR, and its amino acids did a homology of 77% compared with that of Ricinus communis. Protein prediction showed its protein N terminal with a conservative field of 3 Beta-HSD/Epimerase/NAD-binding-10. The coding sequence of BnCCR2 gene was 1291 bp, and could be translated into a 248 amino acids. Blast and protein structure analysis showed that its cDNA sequence had a homology of 74% compared with Populus trichocarpa CCR, and its amino acids had a homology of 81% compared with these of Ricinus communis and Populus trichocarpa. Protein prediction showed its protein N terminal with a conservative field of 3 Beta-HSD/ Epimerase/NAD-binding-4. Three-dimensional structures of BnCCR1 and BnCCR2 had relatively high similarity with CCR genes in petunias, and the similarity was 30.68% and 44.77% respectively. The result of qRT-PCR indicated that the expression of both BnCCR1 and BnCCR2 was different in different periods, while not in different tissues, but the levels of expression in different tissues showed significant differences. We speculated that BnCCR1 and BnCCR2 are different CCR genes in lignin metabolism of Boehmeria nivea, and the result of this study provide a theoretical basis for further exploration of their function in the ramie lignin biosynthesis and regulation.

Key words: Boehmeria nivea, Cinnamoyl-CoA reductase gene, Lignin, Tissue expression

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