矮蓝麦,赤霉酸敏感型,遗传分析,Rht22,圆锥小麦," /> 矮蓝麦,赤霉酸敏感型,遗传分析,Rht22,圆锥小麦,"/> Genetic Analysis on Dwarfing Trait in Landrace Ailanmai of <em>Triticum turgidum</em> L. ssp.<em> turgidum</em>
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Acta Agron Sin ›› 2015, Vol. 41 ›› Issue (12): 1899-1905.doi: 10.3724/SP.J.1006.2015.01899

• RESEARCH ACTIVITIES • Previous Articles     Next Articles

Genetic Analysis on Dwarfing Trait in Landrace Ailanmai of Triticum turgidum L. ssp. turgidum

ZHOU Qiang1,2,3,YUAN Zhong-Wei1,ZHANG Lian-Quan1,NING Shun-Zong1,REN Yong1,2,3,TAO Jun1,2,3,LI Sheng-Rong2,3,LIU Deng-Cai1,*   

  1. 1 Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, China; 2 Mianyang Branch of National Wheat Improvement Center / Mianyang Institute of Agricultural Sciences, Mianyang 621023, China; 3 Key Laboratory of Wheat and Rice Genetics and Breeding of the Ministry of Agriculture, Mianyang 621023, China
  • Received:2015-03-16 Revised:2015-07-20 Online:2015-12-12 Published:2015-08-12

Abstract:

Ailanmai is an important Triticum turgidum ssp. turgidum landrace carrying dwarf gene in China. Its dwarfing trait was found to be sensitive to gibberellic acid. In 2012, we crossed Ailanmai with two high plant landraces, Qinkemai and Ganmai, and obtained their reciprocal F1 hybrids. The genetic analysis was carried out in Mianyang, Sichuan Province using the F1, F2, and F2:3 populations during the 2012–2013 crop seasons. One recessive gene proved to control the dwarfing trait in Ailanmai. Polymorphic simple sequence repeat (SSR) primers associated with plant height were selected through bulked segregant analysis (BSA) and used to identify the F2 individuals. The results indicated that the dwarf gene was located on the short arm of chromosome 7A with a genetic distance of 2.5 cM from marker GWM471. We speculated Rht22 to be the dwarf gene in Ailanmai because the reciprocal F1 and F2 hybrids between Ailanmai and Aiganfanmai (carrying Rht22) exhibited similar distributions in plant height. This speculation was validated with high-through molecular marker analysis. The percentages of identical SNP and DArT markers between Ailanmai and Aiganfanmai were as high as 98.7% and 99.3%, respectively. We conclude that the two landraces might be the same variety a long time ago and became synonymic during their spread accompanying with humanity activities. The dwarf gene in Ailanmai had a moderate or weak effect to reduce plant height in synthetic hexaploid wheat. Thus, it should be utilized by pyramiding other dwarfing genes in wheat dwarfing breeding.

Key words: Ailanmai, Gibberellic acid sensitive, Genetic analysis, Rht22, Triticum turgidum ssp. turgidum

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