矮蓝麦,赤霉酸敏感型,遗传分析,Rht22,圆锥小麦," /> 矮蓝麦,赤霉酸敏感型,遗传分析,Rht22,圆锥小麦,"/> Genetic Analysis on Dwarfing Trait in Landrace Ailanmai of <em>Triticum turgidum</em> L. ssp.<em> turgidum</em>
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Acta Agron Sin ›› 2015, Vol. 41 ›› Issue (12): 1899-1905.doi: 10.3724/SP.J.1006.2015.01899

• RESEARCH ACTIVITIES • Previous Articles     Next Articles

Genetic Analysis on Dwarfing Trait in Landrace Ailanmai of Triticum turgidum L. ssp. turgidum

ZHOU Qiang1,2,3,YUAN Zhong-Wei1,ZHANG Lian-Quan1,NING Shun-Zong1,REN Yong1,2,3,TAO Jun1,2,3,LI Sheng-Rong2,3,LIU Deng-Cai1,*   

  1. 1 Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, China; 2 Mianyang Branch of National Wheat Improvement Center / Mianyang Institute of Agricultural Sciences, Mianyang 621023, China; 3 Key Laboratory of Wheat and Rice Genetics and Breeding of the Ministry of Agriculture, Mianyang 621023, China
  • Received:2015-03-16 Revised:2015-07-20 Online:2015-12-12 Published:2015-08-12

Abstract:

Ailanmai is an important Triticum turgidum ssp. turgidum landrace carrying dwarf gene in China. Its dwarfing trait was found to be sensitive to gibberellic acid. In 2012, we crossed Ailanmai with two high plant landraces, Qinkemai and Ganmai, and obtained their reciprocal F1 hybrids. The genetic analysis was carried out in Mianyang, Sichuan Province using the F1, F2, and F2:3 populations during the 2012–2013 crop seasons. One recessive gene proved to control the dwarfing trait in Ailanmai. Polymorphic simple sequence repeat (SSR) primers associated with plant height were selected through bulked segregant analysis (BSA) and used to identify the F2 individuals. The results indicated that the dwarf gene was located on the short arm of chromosome 7A with a genetic distance of 2.5 cM from marker GWM471. We speculated Rht22 to be the dwarf gene in Ailanmai because the reciprocal F1 and F2 hybrids between Ailanmai and Aiganfanmai (carrying Rht22) exhibited similar distributions in plant height. This speculation was validated with high-through molecular marker analysis. The percentages of identical SNP and DArT markers between Ailanmai and Aiganfanmai were as high as 98.7% and 99.3%, respectively. We conclude that the two landraces might be the same variety a long time ago and became synonymic during their spread accompanying with humanity activities. The dwarf gene in Ailanmai had a moderate or weak effect to reduce plant height in synthetic hexaploid wheat. Thus, it should be utilized by pyramiding other dwarfing genes in wheat dwarfing breeding.

Key words: Ailanmai, Gibberellic acid sensitive, Genetic analysis, Rht22, Triticum turgidum ssp. turgidum

[1]Hedden P. The genes of the Green Revolution. Trends Genet, 2003, 19: 5–9



[2]Mathews K L, Chapman S C, Trethowan R, Singh R P, Crossa J, Pfeiffer W, Ginkel M, DeLacy I. Global adaptation of spring bread and durum wheat lines near-isogenic for major reduced height genes. Crop Sci, 2006, 46: 603–613



[3]万平, 王刚, 刘大钧. 麦类作物矮秆基因遗传学和分子遗传学研究利用进展. 麦类作物, 1998, 18: 9–11



Wan P, Wang G, Liu D J.The research and utilization progress of dwarfing gene genetics and molecular genetics for the Tritical crops. Tritical Crops, 1998, 18: 9–11 (in Chinese)



[4]Ahmad M, Sorrells M E. Distribution of microsatellite alleles linked to Rht8 dwarfing genes in wheat. Euphytica, 2002, 123: 235–240



[5]McIntosh R A, Dubcovsky J, Rogers W J, Morris C, Appels R, Xia X C. Catalogue of gene symbols for wheat: 2013–2014 Supplement. http://www.shigen.nig.ac.jp/wheat/komugi/genes/symbolClassList.jsp



[6]Burner A, Korzun V, Worland A J. Comparative genetic mapping of loci affecting plant height and development in cereals. Euphyt ica, 1998, 100: 245-248



[7]继增, 丁寿康, 李月华, 张辉. 中国小麦的主要矮秆基因及矮源的研究. 中国农业科学, 1992, 25: 1–5



Jia J Z, Ding S K, Li Y H, Zhang H. Studies of main dwarf genes and dwarf resources on Chinese wheat. Sci Agric Sin, 1992, 25: 1–5 (in Chinese with English abstract)



[8]Gale M D, Youssefian S. Dwarfing genes in wheat. In: Russell G E ed. Progress in Plant Breeding (1). Butterworths & London, 1985, pp 1–35



[9] Allan R E, Vogel O A, Craddock J C. Comparative response to gibberellic acid of dwarf, semi-dwarf and standard short and tall winter wheat varieties. Agron J, 1959, 51: 737–740



[10]苏瑾, 彭正松, 杨在君, 魏淑红, 廖明莉, 吴凯. 小麦新矮源矮秆番麦的赤霉素敏感性分析. 西北农业学报, 2012, 21: 28–33



Su J, Peng Z S, Yang Z J, Wei S H, Liao M L, Wu K. Analysis on GA sensitivity of new wheat dwarfing sources Aiganfanmai. Acta Agric Boreali-Occident Sin, 2012, 21: 28–33 (in Chinese with English abstract)



[11]Hoisington D, Khairallah M, Reeves T, Ribaut J M, Skovmand B, Taba S, Warburton M. Plant genetic resources: what can they contribute toward increased crop productivity? Proc Natl Acad Sci USA, 1999, 96: 5937–5943



[12]Ginkel M van, Ogbonnaya F. Novel genetic diversity from synthetic wheats in breeding cultivars for changing production conditions. Field Crops Res, 2007, 104: 86–94



[13]Yang W Y, Liu D C, Li J, Zhang L Q, Wei H T, Hu X R, Zheng Y L, He Z H, Zou Y C. Synthetic hexaploid wheat and its utilization for wheat genetic improvement in China. J Genet Genomics, 2009, 36: 539–546



[14]Warburton M L, Crossa J, Franco J, Kazi M, Trethowan R, Rajaram S, Pfeiffer W, Zhang P, Dreisigacker S, Ginkel M van. Bringing wild relatives back into the family: recovering genetic diversity in CIMMYT improved wheat germplasm. Euphytica, 2006, 149: 289–301



[15]张连全. 小麦异源六倍化过程及其在遗传育种中的应用. 四川农业大学博士学位论文, 四川雅安, 2007



Zhang L Q. Allohexaploidization of common wheat and its application in genetics and breeding. PhD Dissertation of Sichuan Agricultural University, Ya’an, China, 2007 (in Chinese with English abstract)



[16]邹裕春, 杨武云, 朱华忠, 邹裕春, 杨武云, 朱华忠, 杨恩年, 蒲宗君, 伍铃, 张颙, 汤永禄, 黄钢, 李跃建, 何中虎, Singh R, Rajaram S. CIMMYT种质及育种技术在四川小麦品种改良中的利用. 西南农业学报, 2007, 20: 183–190



Zou Y C, Yang W Y, Zhu H Z, Yang E N, Pu Z J, Wu L, Zhang Y, Tang Y L, Huang G, Li Y J, He Z H, Singh R, Rajaram S. Utilization of CIMMYT germplasm and breeding technology in wheat improvement in Sichuan, China. Southwest China J Agric Sci, 2007, 20: 183–190 (in Chinese with English abstract)



[17]董玉琛, 郑殿升. 中国小麦遗传资源. 北京: 中国农业出版社, 2000. pp 20, 43–44



Dong Y C, Zheng D S. Wheat Genetic Resources of China. Beijing: China Agriculture Press, 2000. pp 20, 43–44 (in Chinese)



[18]田良才. 矮兰麦矮秆基因的初步分析. 作物品种资源, 1987, (4): 1–3



Tian L C. Preliminary analysis on the dwarfing gene of Ailanmai. Crop Genet Resour, 1987, (4): 1–3 (in Chinese)



[19]郭保宏.小麦矮秆遗传型对赤霉酸反应的初步研究. 作物品种资源, 1989, (3): 13-15



Guo B H. Preliminary study on wheat dwarf genotype to GAs treatment. Crop Genet Resour, 1989, (3): 13–15 (in Chinese)



[20]Doyle J J, Doyle J L. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull, 1987, 19: 11–15



[21]Michelmore R W, Paran I, Kesseli R V. Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA, 1991, 88: 9828–9832



[22]Gill K S, Gill B S, Endo T R, Boyko E V. Identification and high-density mapping of gene-rich regions in chromosome group 5 of wheat. Genetics, 1996, 143: 1001–1012



[23]刘光欣, 周永红, 郑有良, 杨瑞武, 丁春邦. 矮秆波兰小麦矮秆性状对赤霉酸反应的研究. 四川农业大学学报, 2002, 20: 81–83



Liu G X, Zhou Y H, Zheng Y L, Yang R W, Ding C B. The reaction of hormone gibberellic acid in dwarfing Polish wheat (Triticum turgidum concv. polonicum) from Tulufan, Xinjiang. J Sichuan Agric Univ, 2002, 20: 81–83 (in Chinese with English abstract)



[24]Takeda K. Internode elongation and dwarfism in some Gramineous plants. Gamma Field Symposium, 1977, 16: 1–8



[25]Lanning S P, Martin J M, Stougaard R N, Guillen-Portal F R, Blake N K, Sherman J D, Robbins A M, Kephart K D, Lamb P, Carlson G R, Pumphrey M, Talbert L E. Evaluation of near-isogenic lines for three height-reducing genes in hard red spring wheat. Crop Sci, 2012, 52: 1145–1152



[26]Tang N, Jiang Y, He B R, Hu Y G. The effects of dwarfing genes (Rht-B1b, Rht-D1b, and Rht8) with different sensitivity to GA3 on the coleoptile length and plant height of wheat. Agric Sci China, 2009, 8: 1028–1038



[27]Worland A J, Petrovic S. The gibberellic acid insensitive dwarfing gene from the wheat variety Saitama 27. Euphytica, 1988, 38: 55–63



[28]Flintham J E, Börner A, Worland A J, Gale M D. Optimizing wheat grain yield: effects of Rht (gibberellin-insensitive) dwarfing genes. J Agric Sci, 1997, 128: 11–25



[29]Rebetzke G J, Ellis M H, Bonnett D G, Mickelson B, Condon A G, Richards R A. Height reduction and agronomic performance for selected gibberellin-responsive dwarfing genes in bread wheat (Triticum aestivum L.). Field Crops Res, 2012, 126: 87–96



[30]刘秉华, 王山荭, 杨丽. 中国春小麦株高、育性近等基因系的建立及应用. 遗传, 1999, 21: 31–33



Liu B H, Wang S H, Yang L. Development and utilization of the isogenic lines of plant height and fertility in wheat cv. Chinese Spring. Hereditas (Beijing), 1999, 21: 31–33 (in Chinese with English abstract)



[31]Bomer A, Worland A J, Plaschke J, Schumann E, Law C N. Pleiotropic effects of genes for reduced height (Rht) and day-length insensitivity (Ppd) on yield and its components for wheat grown in middle Europe. Plant Breed, 1993, 111: 204–206



[32]Kertesz Z, Flintham J E, Gale M D. Effects of Rht dwarfing genes on wheat grain yield and its components under eastern European conditions. Cereal Res Commun, 1991, 19: 297–304



[33]Wang Y S, Chen L, Du Y Y, Yang Z Y, Condon A G, Hu Y G. Genetic effect of dwarfing gene Rht13 compared with Rht-D1b on plant height and some agronomic traits in common wheat (Triticum aestivum L.). Field Crops Res, 2014, 162: 39–47



[34]Peng Z S, Li X, Yang Z J, Liao M L. A new reduced height gene found in the tetraploid semi-dwarf wheat landrace Aiganfanmai. Genet Mol Res, 2011, 10: 2349–2357



[35]Peng Z S, Su Z X, Cheng K C. Characterization of dwarfing trait in the tetriploid wheat landrace Aiganfanmai. Wheat Inf Ser, 1999, 89: 7–1
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