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Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (04): 469-481.doi: 10.3724/SP.J.1006.2016.00469

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS •     Next Articles

Cloning and Functional Analysis of a WRKY28-like Gene in Soybean

WANG Ting-Ting,CONG Ya-Hui,LIU Ju-Ge,WANG Ning,SHUAI Qin,LI Yan*,GAI Jun-Yi*   

  1. National Key Laboratory of Crop Genetics and Germplasm Enhancement / National Center for Soybean Improvement / Key Laboratory for Biology and Genetic Improvement of Soybean (General), Ministy of Agriculture /Jiangsu Collaborative Innovation Center for Modern Crop Production,Nanjing Agricultural University, Nanjing 210095, China
  • Received:2015-08-21 Revised:2016-01-11 Online:2016-04-12 Published:2016-01-27
  • Contact: 李艳, E-mail: yanli1@njau.edu.cn; 盖钧镒,E-mail:sir@njau.edu.cn E-mail:wttmrzf@163.com
  • Supported by:

    This research was supported bythe National High-tech R & D Program of China (2013AA102602), theProgram for Changjiang Scholars and Innovative Research Team in University of Ministry of Education of China(PCSIRT13073), the Fundamental Research Funds for the Central Universities, Program for New Century Excellent Talents in University of Ministry of Education of China(NCET-12-0891), Program for Soybean Biology and Genetic Breeding Innovative Research Team of Ministry of Agriculture of China, and Program for High-level Innovative and Entrepreneurial Talents in Jiangsu Province.

Abstract:

WRKY transcription factors play important roles in regulating plant growth and development, andparticipate in plant response to abiotic and biotic stresses.Arabidopsis WRKY28 is an essential transcription factor for plant response to necrotrophic pathogen and abiotic stress. To explore the function of the homologues gene of AtWRKY28 in soybean, we cloned GmWRKY28-like (Glyma.14G028900) and performed bioinformatics analysis. Its sub-cellular localization and tissue expression patternswere further investigated. We also analyzed the expression levels of GmWRKY28-like under ABA, PEG and NaCl stress treatments. Our results showed that the coding DNA sequence (CDS) of GmWRKY28-like gene is 1008 bp in length, encoding 335 amino acids. GmWRKY28-like contains a conserved WRKY domainwith22 serine, one threonine and two tyrosine, without any transmembrane domain or signal peptide. Phylogenetic analysis showed that the WRKY28 from Phaseolus vulgariswas most similar to GmWRKY28-like. GmWRKY28-like was verified to be located in the nucleus. This gene expressed at low levels in root and seed while high levels in true leaf, flower and shoot apical meristem. The 1500 bp upstream region of GmWRKY28-like contains a variety of cis-elements, such as MBS, W box, ABRE, and box-W1, which are involved in the response to biotic and abiotic stresses, andits expression was induced by ABA, PEG and NaCl treatments.In addition, overexpressing GmWRKY28-like in Arabidopsis thaliana enhanced plant tolerance to NaCl.This study would provide reference basis to further study the functions of GmWRKY28-like in soybean tolerance to abiotic stresses.

Key words: GmWRKY28, Phylogenetic analysis, Subcellular location, Abiotic stress

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