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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (03): 454-463.doi: 10.3724/SP.J.1006.2017.00454

• RESEARCH NOTES • Previous Articles     Next Articles

Digital Gene Expression Profiling Analysis of Potato Under Low Temperature Stress

YANG Hui-Ju,GUO Hua-Chun*   

  1. Tuber-Root Crops Research Institute, College of Agronomy and Biotechnology, Yunnan Agriculture University, Kunming650201, China
  • Received:2016-07-06 Revised:2016-11-02 Online:2017-03-12 Published:2016-11-18
  • Contact: 郭华春,E-mail: ynghc@126.com;Tel: 0871-65227728 E-mail:glcyhj@126.com
  • Supported by:

    This study was supported by the National Natural Science Foundation of China(C130407), the China Agriculture Research System(GARS-10) and Specialized Research Fund for science and technology projects of Yunnan province(2013ZA007).

Abstract:

Avariety Cooperation88was used to construct leaf cDNA library of potato treated with –2℃ low temperature stress by digital gene expression profiling (DGE) technology, and detect the differentially expressed genes(DEGs).The results of solexasequencing showed that a total of 28505 DEGs were screened out, 13703 DEGs of whichwere up-regulated and 14802 DEGs were down-regulated. GO enrichment analysis showed that these DEGs were involved insingle-organism metabolic process, oxidation-reduction process, metabolisms of carbohydrate, secondary metabolism and catalytic activity. The KEGGenrichment analysis showed that up-regulated genes were significantly associated withphenylpropanoid, photosynthesis-antennaproteins, carotenoidbiosynthesis, phenylalaninemetabolism, starch and sucrose metabolism pathways, while the down-regulated DEGs mainly enriched in plant hormone signal transduction pathway. The differential expressions of four genes were confirmed by Real-time quantitative PCR, showing that theexpression changes ofthese four genes weregenerally consistent with the results of DGE.

Key words: Potato, Low temperature stress, Gene expression profile, Differentially expressed genes

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