玉米雄穗分枝数主效QTL定位及qTBN5近等基因系构建

doi:10.3724/SP.J.1006.2018.01127

Abstract

Abstract:

Tassel branch number (TBN) is a principal component of maize tassel-related traits important for modern maize breeding and production. To understand the genetic basis of tassel branch number, we adopted 288 markers exhibiting polymorphisms between Zheng 58 and Chang 7-2 to construct the genetic linkage map, and conduct quantitative trait loci (QTLs) analysis using sets of 188 recombinant inbred line (RIL) families derived from the elite maize inbred lines vz. Zheng 58 × Chang 7-2. Five major QTLs controlling tassel branch number within five different chromosomes respectively were validated by using the inclusive composite interval mapping method (ICIM) based on phenotypic data collected in two years. Because qTBN5 located on chromosome bin 5.05 was found to be an important QTL, qTBN5 near-isogenic lines (qTBN5-NILs) were developed by backcrossing and marker-assisted selection, and qTBN5 was further mapped in 13.2 Mb intervals. These findings will advance our understanding of the inheritance basis of TBN, and also facilitate the fine mapping and molecular breeding programs in maize.

Key words: maize, tassel branch number, quantitative trait loci mapping, near-isogenic lines

Zi-Ju DAI,Xin-Tao WANG,Qing YANG,Yan WANG,Ying-Ying ZHANG,Zhang-Ying XI,Bao-Quan LI. Major Quantitative Trait Loci Mapping for Tassel Branch Number and Construction of qTBN5 Near-isogenic Lines in Maize (Zea mays L.)[J].Acta Agronomica Sinica, 2018, 44(8): 1127-1135.

Figures/Tables 7

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年份 Year	郑58 Zheng 58	昌7-2 Chang 7-2	均值 Average	范围 Range	变异系数 Variation coefficient (%)
2015	4.6	17.8	10.7	2.4-22.6	43.60
2016	4.4	17.2	11.2	2.8-21.4	38.83

QTL	染色体 Chromosome	物理区间 Position (Mb)	标记区间 Marker interval	LOD	加性效应 Additive effect	贡献率 R² (%)
qTBN3	3	146.7-175.5	umc1954-bnlg1798	5.06	1.40	13.67
qTBN4	4	169.2-186.7	bnlg2291-umc2041	3.15	-1.01	5.81
qTBN5	5	170.1-200.2	umc2111-bnlg278	9.58	1.88	19.92
qTBN7	7	29.4-83.2	bnlg2233-bnlg1380	4.65	-0.82	16.53
qTBN10	10	127.3-145.5	umc2003-phi323152	6.11	-1.73	9.06

Major Quantitative Trait Loci Mapping for Tassel Branch Number and Construction of qTBN5 Near-isogenic Lines in Maize (Zea mays L.)

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