Welcome to Acta Agronomica Sinica，

Acta Agronomica Sinica ›› 2019, Vol. 45 ›› Issue (7): 1017-1028.doi: 10.3724/SP.J.1006.2019.84142

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Ectopic expression of S-adenosylmethionine decarboxylase (GhSAMDC1) in cotton enhances salt tolerance in Arabidopsis thaliana

TIAN Wen-Gang1,ZHU Xue-Feng1,SONG Wen1,CHENG Wen-Han2,XUE Fei1,ZHU Hua-Guo1,*()   

  1. 1 College of Agronomy, Shihezi University / Key Oasis Eco-Agriculture Laboratory of Production and Group, Shihezi 832003, Xinjiang, China
    2 Jingchu University of Technology, Jingmen 448000, Hubei, China
  • Received:2018-11-05 Accepted:2019-01-19 Online:2019-07-12 Published:2019-03-15
  • Contact: Hua-Guo ZHU E-mail:57530422@qq.com
  • Supported by:
    This study was supported by the National Natural Science Foundation of China(31301363);This study was supported by the National Natural Science Foundation of China(31660427);Science and Technology Development Program of Xinjiang Production and Construction Groups Project(2015AC007);the Natural Science Foundation of Hubei Province(2017CFB162)

RichHTML352

9

PDF

424

Abstract:

Transgenic Arabidopsis thaliana (GhSAMDC1) was used to study the effect of overexpression of GhSAMDC1 on salt tolerance of Arabidopsis thaliana seedlings, Contents of endogenous polyamines, hydrogen peroxide (H2O2), malondialdehyde (MDA), and chlorophyll, ion permeability, antioxidant enzymes (SOD, CAT, POD) activities and expression levels were investigated under salt stress. The overexpression of GhSAMDC1 decreased the content of endogenous putrescine (Put) and increased spermidine (Spd) and spermine (Spm) contents in Arabidopsis thaliana. Under salt stress, the expression levels of spermidine synthase (AtSPDS1, AtSPDS2) and spermine synthase (AtSPMS) in transgenic lines were significantly higher than those in wild type, the contents of Spd and Spm were further increased, and the contents of H2O2, MDA, chlorophyll, and ion permeability were obviously decreased. Compared with the wild type, Transgenic lines had no remarkable difference in peroxidase (POD) activity, but significantly higher superoxide dismutase (SOD) and catalase (CAT) activities, with the same change trend as their expression levels. Therefore, GhSAMDC1 increased the contents of Spd and Spm of transgenic plants by increasing the expression of genes related to Spd and Spm synthesis under salt stress, Spd and Spm directly or indirectly increased the activity of enzymes related to antioxidant system, and enhanced the salt tolerance of Arabidopsis thaliana by scavenging H2O2 and other reactive oxygen species.

Key words: Arabidopsis thaliana, cotton S-adenosylmethionine decarboxylase gene, salt stress, antioxidant enzyme

Fig. 1

Phylogenetic analysis of homologous proteins of upland cotton GhSAMDC1 and other species and identification of transgenic Arabidopsis thaliana A: phylogenetic analysis of homologous proteins of upland cotton GhSAMDC1 and other species; B: expression vector of GhSDAMC1; C: DNA identification of transgenic Arabidopsis thaliana (GhSAMDC1), M, 1, 2, 3, 4, and 5 were marker, transgenic line 1-4, transgenic line 1-12, transgenic line 1-14, positive control, and negative control, respectively, the size of the target gene fragment was 1035 bp; D: expression analysis of GhSAMDC1 in Arabidopsis thaliana."

Fig. 2

Effect of overexpression of GhSAMDC1 on endogenous polyamines contents in Arabidopsis thaliana A-D: total endogenous polyamines, Put, Spd, and Spm contents in wild type and transgenic lines. The contents of endogenous polyamines in Arabidopsis thaliana leaves were determined by high performance liquid chromatography (HPLC) after normal cultured for 30 days, and Duncan method was used to test the difference significance (* P < 0.05, ** P < 0.01), the results were represented by mean (±standard deviation), the above experiments were repeated three times biologically and three times technically in each experiment."

Supplementary Fig. 1

Expression analysis of GhSAMDC1 in cotton under 300 mmol L-1 NaCl treatment 0, 1, 3, 6, 12, 24, 48, and72 represent cotton seedlings (YZ-1) after normal culture for 30 days treated with 300 mmol L-1 NaCl for 0, 1, 3, 6, 12, 24, 48, and 72 hours, respectively. Samples were taken at 0, 1, 3, 6, 12, 24, 48, and 72 hours of treatments to detect the relative expression of GhSAMDC1 in different treatment periods. The above experiments were repeated three times biologically and three times technically in each experiment."

Fig. 3

Effect of overexpression of GhSAMDC1 on salt tolerance in Arabidopsis thaliana A: schematic diagram of seed distribution; B-C: phenotypes of wild type and transgenic Arabidopsis thaliana cultivated under normal and salt stress for 15 days; D-E: fresh weight and leaf number of wild type and transgenic Arabidopsis thaliana after normal culture for 15 days; F-H: survival rate, fresh weight and leaf number of wild type and transgenic Arabidopsis thaliana after 100 mmol L-1 NaCl treatment for 15 days. Duncan method was used to test the difference significance (* P < 0.05, ** P < 0.01), the results were represented by mean (±standard), the above experiments were repeated three times biologically and three times technically in each experiment."

Fig. 4

Effects of overexpression of GhSAMDC1 on chlorophyll contents in Arabidopsis thaliana leaves under salt stress A-C: chlorophyll content of wild type and transgenic lines under salt stress. The leaves of Arabidopsis thaliana were selected to detect the content of chlorophyll a and b after treated with 100 mmol L-1 NaCl for 15 days. Duncan method was used to test the difference significance (*P < 0.05, **P < 0.01), the results were represented by mean (±standard deviation), the above experiments were repeated three times biologically and three times technically in each experiment."

Fig. 5

Effects of overexpression of GhSAMDC1 on endogenous polyamine content in Arabidopsis thaliana under salt stress A-D: comparison of total endogenous polyamines, Put, Spd, and Spm contents between wild type and transgenic lines under normal and salt stress conditions. The contents of endogenous polyamines in Arabidopsis thaliana leaves were determined by high performance liquid chromatography (HPLC) after normal culture and treatment with 100 mmol L-1 NaCl for 15 days. Duncan’s method was used to test the difference significance (* P < 0.05, ** P < 0.01), the results were represented by mean (±standard deviation), the above experiments were repeated three times biologically and three times technically in each experiment."

Fig. 6

Effects of overexpression of GhSAMDC1 on endogenous gene expression in Arabidopsis thaliana under salt stress A-C: relative expression of AtSPDS1, AtSPDS2, and AtSPMS in wild type and transgenic Arabidopsis thaliana. The contents of endogenous polyamines in Arabidopsis thaliana leaves were determined by high performance liquid chromatography (HPLC) after treated with 100 mmol L-1 NaCl for 15 days. Duncan’s method was used to test the difference significance (* P < 0.05, ** P < 0.01), the results were represented by mean (±standard deviation), the above experiments were repeated three times biologically and three times technically in each experiment."

Fig. 7

Effects of overexpression of GhSAMDC1 on contents of H2O2, MDA, and ion permeability in Arabidopsis thaliana leaves under salt stress A: DAB staining of wild type and transgenic lines under salt stress; B-D: contents of H2O2, MDA and ion permeability of wild type and transgenic lines under salt stress. The leaves of Arabidopsis thaliana were stained with DAB, and the contents of H2O2, MDA and ion permeability were measured after treated with 100 mmol L-1 NaCl for 15 days. Duncan’s method was used to test the difference significance (* P < 0.05, ** P < 0.01), the results were represented by mean (±standard deviation), the above experiments were repeated three times biologically and three times technically in each experiment."

Fig. 8

Effects of overexpression of GhSAMDC1 on activity and expression of antioxidant enzymes in Arabidopsis thaliana under salt stress A-C: enzyme activity of CAT, SOD, and POD in wild type and transgenic lines under salt stress; D-F: analysis of CAT, SOD, and POD expression in wild type and transgenic lines under salt stress. The leaves of Arabidopsis thaliana were selected to detect the activity and relative expression of CAT, SOD, and POD after treated with 100 mmol L-1 NaCl for 15 days, respectively. Duncan’s method was used to test the difference significance (* P < 0.05, ** P < 0.01), the results were represented by mean (±standard deviation), the above experiments were repeated three times biologic ally and three times technically in each experiment."

[1] Alcázar R, Altabella T, Marco F, Bortolotti C, Reymond M, Koncz C, Carrasco P, Tiburcio A F . Polyamines: molecules with regulatory functions in plant abiotic stress tolerance. Planta, 2010,231:1237-1249.
doi: 10.1007/s00425-010-1130-0
[2] Kusano T, Berberich T C, Takahashi Y . Polyamines: essential factors for growth and survival. Planta, 2008,228:367-381.
doi: 10.1007/s00425-008-0772-7
[3] Slocum R D, Kaur-Sawhney R, Galston A W . The physiology and biochemistry of polyamines in plants. Arch Biochem Biophys, 1984,235:283-303.
doi: 10.1016/0003-9861(84)90201-7
[4] Soo W, Soo K, Woo K, Ky P . Constitutive S-adenosylmethionine decarboxylase gene expression increases drought tolerance through inhibition of reactive oxygen species accumulation in Arabidopsis. Planta, 2014,239:979-988.
[5] Torrigiani P, Scaramagli S, Ziosi V, Mayer M, Biondi S . Expression of an antisense Datura stramonium S-adenosylmethionine decarboxylase cDNA in tobacco: changes in enzyme activity, putrescine-spermidine ratio, rhizogenic potential, and response to methyl jasmonate. J Plant Physiol, 2005,162:559-571.
[6] Sinha R, Rajam M V . RNAi silencing of three homologues of S-adenosylmethionine decarboxylase gene in tapetal tissue of tomato results in male sterility. Plant Mol Biol, 2013,82:169-180.
doi: 10.1007/s11103-013-0051-2
[7] 路玉兰, 孙艳香, 冯雪, 赵学良 . 百脉根S-腺苷甲硫氨酸脱羧酶基因克隆与表达分析. 华北农学报, 2013,28(2):78-85.
doi: 10.3969/j.issn.1000-7091.2013.02.015
Lu Y L, Sun Y X, Feng X, Zhao X L . Cloning and expression analysis of S-adenosylmethionine decarboxylase gene from Lotus corniculatus L. Acta Agric Boreali-Sin, 2013,28(2):78-85 (in Chinese with English abstract).
doi: 10.3969/j.issn.1000-7091.2013.02.015
[8] Peng X J, Zhang L X, Zhang L X, Liu Z J, Cheng L Q, Yang Y, Shen S H, Chen S Y, Liu G S . The transcriptional factor LcDREB2 cooperates with LcSAMDC2 to contribute to salt tolerance in Leymus chinensis. Plant Cell Tissue Organ Culture, 2013,113:245-256.
[9] 王凡龙, 朱华国, 程文翰, 刘永昌, 成新琪, 孙杰 . 棉花S-腺苷蛋氨酸脱羧酶基因(GhSAMDC2/3/4)的克隆及其诱导表达分析. 棉花学报, 2015,27:176-183.
Wang F L, Zhu H G, Cheng W H, Liu Y C, Cheng X Q, Sun J . Cloning and induced expression analysis ofGhSAMDC2/3/4 in cotton(Gossypium hirsutum L.). Cotton Sci, 2015,27:176-183 (in Chinese with English abstract).
[10] 张梅, 王然, 马春晖, 段艳欣, 李鼎立 . 杜梨S-腺苷甲硫氨酸脱羧酶基因的克隆与生物信息学分析. 华北农学报, 2013,28(1):82-87.
doi: 10.3969/j.issn.1000-7091.2013.01.016
Zhang M, Wang R, Ma C H, Duan Y X, Li D L . Cloning and bioinformatics analysis of S-adenosylmethionine decarboxylase gene in Pyrus betulaefolia Bge. Acta Agric Boreali-Sin, 2013,28(1):82-87 (in Chinese with English abstract).
doi: 10.3969/j.issn.1000-7091.2013.01.016
[11] 文乐, 黄诚梅, 邓智年, 曹辉庆, 魏源文, 李楠, 吴凯朝 . 甘蔗S-腺苷蛋氨酸脱羧酶基因Sc-SAMDC3的克隆和表达分析. 南方农业学报, 2015,46:1931-1936.
Wen Y, Huang C M, Deng Z N, Cao H Q, Li N, Wu K C . Molecular cloning of sugarcane S-adenosylmethionine decarboxylase gene (Sc-SAMDC3) and its expression analysis. J Southern Agric, 2015,46:1931-1936 (in Chinese with English abstract).
[12] 王小利, 刘晓霞, 王舒颖, 杨义成, 吴佳海 . 高羊茅腺苷甲硫氨酸脱羧酶基因FaSAMDC的克隆与差异表达分析. 草业学报, 2011,20(4):169-179.
doi: 10.11686/cyxb20110421
Wang X L, Liu X X, Wang S Y, Yang Y C, Wu J H . Cloning and differential expression analysis of S-adenosylmethionine decarboxylase gene FaSAMDC in tall fescue. Acta Pratac Sin, 2011,20(4):169-179 (in Chinese with English abstract).
doi: 10.11686/cyxb20110421
[13] Liu Z J, Liu P P, Qi D M, Peng X J, Liu G S . Enhancement of cold and salt tolerance of Arabidopsis by transgenic expression of the S-adenosylmethionine decarboxylase gene from Leymus chinensis. J Plant Physiol, 2017,211:90-99.
[14] Cheng L, Zou Y J, Ding S L, Zhang J J, Yu X L, Cao J S, Lu G . Polyamine accumulation in transgenictomato enhances the tolerance to high temperature stress. Chin Bull Bot, 2009,51:489-499.
[15] Chen M, Chen J J, Fang J Y, Guo Z F, Lu S Y . Down-regulation of S-adenosylmethionine decarboxylase genes results in reduced plant length, pollen viability, and abiotic stress tolerance. Plant Cell Tissue Organ Culture, 2014,116:311-322.
doi: 10.1007/s11240-013-0405-0
[16] Elsayed A I, Rafudeen M S, El-hamahmy M A M, Odero D C, Sazzad H M . Enhancing antioxidant systems by exogenous spermine and spermidine in wheat (Triticum aestivum) seedlings exposed to salt stress. Funct Plant Biol, 2018,45:745, doi: 10.1071/FP17127.
[17] Wu J Q, Shu S, Li C C, Sun J, Guo S R . Spermidine-mediated hydrogen peroxide signaling enhances the antioxidant capacity of salt-stressed cucumber roots. Plant Physiol Biochem, 2018,128:152-162.
doi: 10.1016/j.plaphy.2018.05.002
[18] Zhou H, Guo S R, An Y H, Shan X, Wang Y, Shu S, Sun J . Exogenous spermidine delays chlorophyll metabolism in cucumber leaves (Cucumis sativus L.) under high temperature stress. Acta Physiol Planta, 2016,38:224.
[19] Liu J, Yu B J, Liu Y L . Effects of spermidine and spermine levels on salt tolerance associated with tonoplast H +-ATPase and H +-PPase activities in barley roots . Plant Growth Regul, 2006,49:119-126.
doi: 10.1007/s10725-006-9001-1
[20] Duan J J, Guo S R, Fan H F, Wang S P, Kang Y Y . Effects of salt stress on proline and polyamine metabolisms in the roots of cucumber seedlings. Acta Bot Boreali-Occident Sin, 2006,26:2486-2492.
[21] Halliwell B . Reactive species and antioxidants. Redox biology is a fundamental theme of aerobic life. Plant Physiol, 2006,141:312-322.
doi: 10.1104/pp.106.077073
[22] Ma C Q, Wang Y G, Gu D, Nan J D, Chen S X, Li H Y . Overexpression of S-adenosyl-l-methionine synthetase 2 from sugar beet M14 increased Arabidopsis tolerance to salt and oxidative stress. Int J Mol Sci, 2017,18:e847.
[23] Li J M, Hu L P, Zhang L, Pan X B, Hu X H . Exogenous spermidine is enhancing tomato tolerance to salinity-alkalinity stress by regulating chloroplast antioxidant system and chlorophyll metabolism. BMC Plant Biol, 2015,15:303, doi: 10.1186/s12870- 015-0699-7.
doi: 10.1186/s12870-015-0699-7
[24] Asada K . THE WATER-WATER CYCLE IN CHLOROPLASTS: Scavenging of active oxygens and dissipation of excess photons. Annu Rev Plant Physiol Plant Mol Biol, 1999,50:601-639.
doi: 10.1146/annurev.arplant.50.1.601
[25] Williamson G B, Richardson D . Bioassays for allelopathy: Measuring treatment responses with independent controls. J Chem Ecol, 1988,14:181-187.
doi: 10.1007/BF01022540
[26] Zhao F Y, Guo S L, Zhang H, Zhao Y X . Expression of yeast SOD2, in transgenic rice results in increased salt tolerance. Plant Sci, 2006,170:216-224.
doi: 10.1016/j.plantsci.2005.08.017
[27] Puyang X H, An M Y, Han L B, Zhang X Z . Protective effect of spermidine on salt stress induced oxidative damage in two Kentucky bluegrass (Poa pratensis L.) cultivars. Ecotoxicol Environ Saf, 2015,117:96-106.
[28] Li S, Han J, Qiang Z . The effect of exogenous spermidine concentration on polyamine metabolism and salt tolerance in Zoysiagrass (Zoysia japonica Steud) subjected to short-term salinity stress. Front Plant Sci, 2016,7:1221, doi: 10.3389/fpls. 2016.01221.
[29] Radhakrishnan R . Ameliorative effects of spermine against osmotic stress through antioxidants and abscisic acid changes in soybean pods and seeds. Acta Physiol Planta, 2013,35:263-269.
doi: 10.1007/s11738-012-1072-1
[30] Zrig A, Tounelti T, Vadel A M, Mohamed H B, Valero D, Serrano M, Chtara C, Khemira H . Possible involvement of polyphenols and polyamines in salt tolerance of almond rootstocks. Plant Physiol Biochem, 2011,49:1313-1322.
doi: 10.1016/j.plaphy.2011.08.009
[31] 程文翰, 朱华国, 李鹏飞, 王凡龙, 朱守鸿, 赵兰杰, 郭丽雪, 孙杰 . 棉花多胺HPLC的测定方法优化及其在体细胞胚胎发生过程中的变化规律. 棉花学报, 2014,26:138-144.
Cheng W H, Zhu H G, Li P F, Wang F L, Zhu S H, Zhao L J, Guo L X, Sun J . Method optimization of polyamine content by high-performance liquid chromatography and its changes in the process of somatic embryogenesis in cotton. Cotton Sci, 2014,26:138-144 (in Chinese with English abstract).
[32] Cheng W H, Wang F H, Cheng X Q, Zhu Q H, Sun Y Q, Zhu H G, Sun J . Polyamine and its metabolite H2O2 play a key role in the conversion of embryogenic callus into somatic embryos in upland cotton (Gossypium hirsutum L.). Front Plant Sci, 2015,6:1063, doi: 10.3389/fpls.2015.01063.
[33] Li C, Zhang Y N, Zhang K, Guo D L, Cui B M, Wang X Y, Huang X Z . Promoting flowering, lateral shoot outgrowth, leaf development, and flower abscission in tobacco plants overexpressing cotton FLOWERING LOCUS T (FT)-like gene GhFT1. Front Plant Sci, 2015,6:454, doi: 10.3389/fpls.2015.00454.
[34] Crumbliss A L, Perine S C, Stonehuerner J, Tubergen K R, Zhao J, Henkens R W, Q’Daly J P . Colloidal gold as a biocompatible immobilization matrix suitable for the fabrication of enzyme electrodes by electrodeposition. Biotechnol Bioeng, 1992,40:483-490.
doi: 10.1002/(ISSN)1097-0290
[35] 朱珍 . 赤霉素调控采后番茄果实抗冷机制研究. 中国农业科学院硕士学位论文, 北京, 2016.
Zhu Z . The Mechanism of Gibberellins in Regulation of Chilling Tolerance of Postharvest Tomato Fruit. MS Thesis of Chinese Academy of Agricultural Sciences, Beijing, China, 2016 (in Chinese with English abstract).
[36] Livak K J, Schmittgen T D . Analysis of relative gene expression data using real-time quantitative PCR and the 2 -ΔΔCT method . Methods, 2001,25:402-408.
doi: 10.1006/meth.2001.1262
[37] Hao Y J, Zhang Z L, Kitashiba H, Honda C, Ubi B, Kita M, Moriguchi T . Molecular cloning and functional characterization of two apple S-adenosylmethionine decarboxylase genes and their different expression in fruit development, cell growth and stress responses. Gene, 2005,350:41-50.
doi: 10.1016/j.gene.2005.01.004
[38] Roy M, Wu R . Overexpression of S-adenosylmethionine decarboxylase gene in rice increases polyamine level and enhances sodium chloride-stress tolerance. Plant Sci, 2002,163:987-992.
doi: 10.1016/S0168-9452(02)00272-8
[39] Waie B, Rajam M V . Effect of increased polyamine biosynthesis on stress responses in transgenic tobacco by introduction of human S-adenosylmethionine gene. Plant Sci, 2003,164:722-734.
[40] Sanchez D H, Cuevas J C, Chiesa M A, Ruiz O A . Free spermidine and spermine content in Lotus glaber under long-term salt stress. Plant Sci, 2005,168:541-546.
[41] Zapata P J, Serrano M, Pretel M T, Amoros A, Botella M A . Polyamines and ethylene changes during germination of different plant species under salinity. Plant Sci, 2004,167:781-788.
doi: 10.1016/j.plantsci.2004.05.014
[42] Liu H P, Dong B H, Zhang Y Y, Liu Z P, Liu Y L . Relationship between osmotic stress and the levels of free, conjugated and bound polyamines in leaves of wheat seedlings. Plant Sci, 2004,166:1261-1267.
doi: 10.1016/j.plantsci.2003.12.039
[43] Wi S J, Kim W T, Park K Y . Overexpression of camation S-adenosylmethionine decarboxylase gene generate a broad- spectrum tolerance to abiotic stresses in transgenenic tobacco plants. Plant Cell Rep, 2006,25:1111-1121.
[44] Liu H P, Zhu Z X, Liu Y L . Response of bound polyamines in the thylakoid membrane of wheat seedling to osmotic stress. Seed, 2007,26:58-60.
[45] Ha H C, Sirisoma N S, Kuppusamy P, Zweier J L, Woster P M, Casero R A . The natural polyamine spermine functions directly as a free radical scavenger. Proc Natl Acad Sci USA, 1998,95:11140-11145.
doi: 10.1073/pnas.95.19.11140
[46] Tiburcio A F, Besford R T, Capell T, Borrell A, Testillano P S, Risueno M C . Mechanisms of polyamine action during senescence responses induced by osmotic stress. J Exp Bot, 1994,45:1789-1800.
doi: 10.1093/jxb/45.12.1789
[1] YAN Jia-Qian, GU Yi-Biao, XUE Zhang-Yi, ZHOU Tian-Yang, GE Qian-Qian, ZHANG Hao, LIU Li-Jun, WANG Zhi-Qin, GU Jun-Fei, YANG Jian-Chang, ZHOU Zhen-Ling, XU Da-Yong. Different responses of rice cultivars to salt stress and the underlying mechanisms [J]. Acta Agronomica Sinica, 2022, 48(6): 1463-1475.
[2] LEI Xin-Hui, WAN Chen-Xi, TAO Jin-Cai, LENG Jia-Jun, WU Yi-Xin, WANG Jia-Le, WANG Peng-Ke, YANG Qing-Hua, FENG Bai-Li, GAO Jin-Feng. Effects of soaking seeds with MT and EBR on germination and seedling growth in buckwheat under salt stress [J]. Acta Agronomica Sinica, 2022, 48(5): 1210-1221.
[3] MENG Ying, XING Lei-Lei, CAO Xiao-Hong, GUO Guang-Yan, CHAI Jian-Fang, BEI Cai-Li. Cloning of Ta4CL1 and its function in promoting plant growth and lignin deposition in transgenic Arabidopsis plants [J]. Acta Agronomica Sinica, 2022, 48(1): 63-75.
[4] DAI Liang-Xiang, XU Yang, ZHANG Guan-Chu, SHI Xiao-Long, QIN Fei-Fei, DING Hong, ZHANG Zhi-Meng. Response of rhizosphere bacterial community diversity to salt stress in peanut [J]. Acta Agronomica Sinica, 2021, 47(8): 1581-1592.
[5] LI Zeng-Qiang, DING Xin-Chao, LU Hai, HU Ya-Li, YUE Jiao, HUANG Zhen, MO Liang-Yu, CHEN Li, CHEN Tao, CHEN Peng. Physiological characteristics and DNA methylation analysis under lead stress in kenaf (Hibiscus cannabinus L.) [J]. Acta Agronomica Sinica, 2021, 47(6): 1031-1042.
[6] LIU Ya-Wen, ZHANG Hong-Yan, CAO Dan, LI Lan-Zhi. Prediction of drought and salt stress-related genes in rice based on multi-platform gene expression data [J]. Acta Agronomica Sinica, 2021, 47(12): 2423-2439.
[7] Hui LI, De-Fang LI, Yong DENG, Gen PAN, An-Guo CHEN, Li-Ning ZHAO, Hui-Juan TANG. Cloning of the key enzyme gene HcTPPJ in trehalose biosynthesis of kenaf and its expression in response to abiotic stress in kenaf [J]. Acta Agronomica Sinica, 2020, 46(12): 1914-1922.
[8] LI Run-Zhi, JIN Qing, LI Zhao-Hu, WANG Ye, PENG Zhen, DUAN Liu-Sheng. Salicylic acid improved salinity tolerance of Glycyrrhiza uralensis Fisch during seed germination and seedling growth stages [J]. Acta Agronomica Sinica, 2020, 46(11): 1810-1816.
[9] CHEN Xiao-Jing,LIU Jing-Hui,YANG Yan-Ming,ZHAO Zhou,XU Zhong-Shan,HAI Xia,HAN Yu-Ting. Effects of salt stress on physiological indexes and differential proteomics of oat leaf [J]. Acta Agronomica Sinica, 2019, 45(9): 1431-1439.
[10] LI Xu-Kai,LI Ren-Jian,ZHANG Bao-Jun. Identification of rice stress-related gene co-expression modules by WGCNA [J]. Acta Agronomica Sinica, 2019, 45(9): 1349-1364.
[11] Ning HE,Xue-Yang WANG,Liang-Zi CAO,Da-Wei CAO,Yu LUO,Lian-Zi JIANG,Ying MENG,Chun-Xu LENG,Xiao-Dong TANG,Yi-Dan LI,Shu-Ming WAN,Huan LU,Xu-Zhen CHENG. Effects of photoperiods and temperatures on physiological characteristics and chlorophyll synthesis precursors of adzuki bean seedlings [J]. Acta Agronomica Sinica, 2019, 45(3): 460-468.
[12] Hua-Ying MAO,Feng LIU,Wei-Hua SU,Ning HUANG,Hui LING,Xu ZHANG,Wen-Ju WANG,Cong-Na LI,Han-Chen TANG,Ya-Chun SU,You-Xiong QUE. A Sugarcane Phosphatidylinositol Transfer Protein Gene ScSEC14 Responds to Drought and Salt Stresses [J]. Acta Agronomica Sinica, 2018, 44(6): 824-835.
[13] Xiang ZHAO,Zi-Yi ZHU,Xiao-Nan WANG,Shi-Chao MU,Xiao ZHANG. Functional Analysis of Hypocotyl Phototropism Modulated by RPT2-Interacting Protein RIP1 in Arabidopsis thaliana L. [J]. Acta Agronomica Sinica, 2018, 44(12): 1802-1808.
[14] Guang-Long ZHU,Cheng-Yu SONG,Lin-Lin YU,Xu-Bing CHEN,Wen-Fang ZHI,Jia-Wei LIU,Xiu-Rong JIAO,Gui-Sheng ZHOU. Alleviation Effects of Exogenous Growth Regulators on Seed Germination of Sweet Sorghum under Salt Stress and Its Physiological Basis [J]. Acta Agronomica Sinica, 2018, 44(11): 1713-1724.
[15] Li-Li WAN, Zhuan-Rong WANG, Qiang XIN, Fa-Ming DONG, Deng-Feng HONG, Guang-Sheng YANG. Enhanced Accumulation of BnA7HSP70 Molecular Chaperone Binding Protein Improves Tolerance to Drought Stress in Transgenic Brassica napus [J]. Acta Agronomica Sinica, 2018, 44(04): 483-492.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No.12 Zhongguancun South Street, Beijing 100081,China Email:xbzw@chinajournal.net.cn
Supported by Beijing Magtech Co.Ltd