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Acta Agronomica Sinica ›› 2019, Vol. 45 ›› Issue (9): 1416-1430.doi: 10.3724/SP.J.1006.2019.84169

• TILLAGE & CULTIVATION· PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Screening and comprehensive evaluation of aluminum-toxicity tolerance during seed germination in Brassca napus

GAO Huan-Huan,YE Sang,WANG Qian,WANG Liu-Yan,WANG Rui-Li,CHEN Liu-Yi,TANG Zhang-Lin,LI Jia-Na,ZHOU Qing-Yuan(),CUI Cui()   

  1. College of Agronomy and Biotechnology, Southwestern University, Chongqing 400715, China
  • Received:2018-12-12 Accepted:2019-04-15 Online:2019-09-12 Published:2019-05-08
  • Contact: Qing-Yuan ZHOU,Cui CUI E-mail:zhouqy2005@163.com;cuigreeny@163.com
  • Supported by:
    This study was supported by the China Agriculture Research System(CARS-12);the National Key Research and Development Project(2018YFD0100505);the Science and Technology Committee of Chongqing(cstc2016shmszx80015)

Abstract:

Seed germination is an important stage of plant morphogenesis in rapeseed. Aluminum toxicity is one of the main factors affecting seed germination in acidic soil. Therefore, it is important for rapeseed production and research to screen Al-tolerant germplasms during seed germination. In this study, five Brassica napus cultivars (lines) were used to screen the optimum treatment concentration of Al toxicity at germination stage, which was determined at 90 μg mL -1. The germination vigor, germination rate, fresh weight, dry weight, root length and bud length of 148 cultivars (lines) of Brassica napus were measured at germination stage. The comprehensive evaluation value (A value), average subordinate function value (ASF value), weighted tolerance coefficient (WAC value), correlation coefficient, frequency, principal component, grey relational degree, clustering and stepwise regression were used to identify the Al tolerance at germination stage. A comprehensive evaluation model of Al tolerance at germination stage was established, suitable evaluation and identification indexes were selected. There were significant differences in the germination parameters of 148 Brassica napus germplasm, and aluminum-tolerant Brassica napus varieties (lines) during germination stage were screened, including 01188, WH-20, A109, and Jiayu 31 peng. The results of grey correlation and regression analysis indicated that the root length, bud length, fresh weight, germination rate and germination potential measured at the germination stage of rapeseed, and the A value of the indices estimated by regression equation, could be preliminarily judged the aluminum tolerance of rape germplasm.

Key words: Brassica napus L, germination stage, aluminum toxicity stress, screening for germplasm resources, comprehensive evaluation

Table 1

Changes of root length of different varieties under different aluminum toxicity stress concentrations (cm)"

浓度Concentration
(μg mL-1)
品种(系) Cultivar (line) 均值Mean 较对照变化Comparison with the control (%)
ZS11 D011 D363 D016 D064
0 9.39±0.131 a 7.97±0.345 a 9.17±0.390 a 5.39±0.401 a 9.24±0.259 a 8.23 0
30 8.35±0.372 ab 8.17±0.237 a 9.04±0.068 a 5.17±0.947 a 7.66±0.119 a 7.68 -6.72
60 6.78±1.084 bc 7.34±0.377 a 5.90±0.700 b 5.34±0.541 a 7.48±0.422 a 6.57 -20.24
90 6.97±0.240 bc 6.96±0.489 a 6.10±0.493 b 4.35±0.127 a 7.95±0.631 a 6.47 -21.45
120 5.71±0.699 c 4.65±0.560 b 3.81±0.468 c 2.79±0.244 b 4.00±0.952 b 4.19 -49.07
150 3.47±0.623 d 1.61±0.263 c 3.82±0.223 c 1.68±0.287 b 4.10±0.122 b 2.50 -69.65
240 2.10±0.123 d 2.39±0.347 c 3.83±0.745 c 1.79±0.546 b 2.32±0.149 b 2.27 -72.41

Table 2

Changes of various indexes of rapeseed varieties under aluminum toxicity stress"

参数
Parameter
根长
RL (cm)
芽长
SL (cm)
发芽势
GV (%)
发芽率
GR (%)
鲜重
FW (g)
干重
DW (g)
对照 最大值Max. 12.75 3.09 100.00 100.00 0.55 0.06
Control 最小值Min. 1.79 0.80 12.50 40.00 0.14 0.02
均值Average 6.72** 1.65** 64.88** 69.05** 0.33** 0.04**
标准差SE 1.89 0.41 19.80 17.12 0.08 0.01
变异系数CV (%) 28.08 24.98 30.52 24.79 24.90 21.60
处理 最大值Max. 8.68 3.29 100.00 100.00 0.47 0.09
Treatment 最小值Min. 0.49 0.86 7.50 15.00 0.12 0.02
均值Average 4.55** 1.73** 68.65** 72.18** 0.28** 0.03**
标准差SE 1.50 0.42 20.17 18.66 0.08 0.01
变异系数CV (%) 32.96 24.49 29.38 25.85 27.71 26.41
较对照变化 均值Average (%) -32.36 4.86 5.81 4.53 -15.60 -4.26
Comparison with the control 变异系数CV (%) 17.38 -1.96 -3.73 4.26 11.28 22.24

Fig. 1

Frequency distribution of tolerance coefficients of different indicators in Brassica napus GV: germination vigor; GR: germination rate; RL: root length; SL: shoot length; FW: fresh weight; DW: dry weight."

Table 3

Aluminum toxicity stress tolerance coefficient of each index of rapeseed (AC value) "

参数
Parameter
发芽势
GV (%)
发芽率
GR (%)
鲜重
FW (g)
干重
DW (g)
根长
RL (cm)
芽长
SL (cm)
最大值Max. 3.125 2.250 1.721 1.613 1.874 1.486
最小值Min. 0.278 0.316 0.394 0.710 0.271 0.698
均值Average 1.093 ** 1.064 ** 0.857** 0.959 ** 0.705 ** 1.059 **
标准差SE 0.325 0.258 0.191 0.121 0.257 0.137
变异系数CV (%) 29.717 24.263 22.249 12.650 36.528 12.888

Table 4

Correlation coefficients of aluminum toxicity stress tolerance coefficient among various indexes of rapeseed"

测定指标
Index
发芽势
GV (%)
发芽率
GR (%)
鲜重
FW (g)
干重
DW (g)
根长
RL (cm)
芽长
SL (cm)
发芽势 GV 1
发芽率 GR 0.888** 1
鲜重 FW 0.026 0.008 1
干重 DW -0.167 -0.159 0.407** 1
根长 RL 0.157* 0.227** 0.420** 0.116 1
芽长 SL -0.032 -0.036 0.246** 0.128 0.154* 1

Table 5

Characteristic value, contribution rate, and principal component characteristic value of each comprehensive index"

主成分Principal
component
特征值Eigen value 贡献率
Variance
contribution ratio
累计贡献率Accumulated variance contribution ratio 特征向量值 Eigen vector of measured indicators
发芽势
GV
发芽率
GR
鲜重
FW
干重
DW
根长
RL
芽长
SL
1 2.005 33.418 33.418 0.944 0.956 0.090 -0.230 0.372 -0.015
2 1.771 29.522 62.940 -0.087 -0.068 0.837 0.652 0.616 0.505
3 0.885 14.747 77.688 -0.021 -0.031 -0.154 -0.407 -0.048 0.832
4 0.763 12.723 90.411 0.216 0.157 -0.039 0.511 -0.618 0.219

Table 6

ASF value, A value, and WAC value sequence of rapeseed variety resources under aluminum toxicity stress"

品种(系)
Cultivar (line)
材料来源
Source
ASF值
ASF value
排序
Order
A值
A value
排序
Order
WAC值
WAC value
排序
Order
SWU7 重庆 Chongqing 0.35 59 0.12 58 0.90 88
SWU36 重庆 Chongqing 0.27 125 -0.61 129 0.79 136
SWU49 重庆 Chongqing 0.34 70 -0.07 80 0.90 91
SWU64 重庆 Chongqing 0.45 11 0.93 12 1.11 11
SWU69 重庆 Chongqing 0.40 25 0.44 26 1.00 33
SWU71 重庆 Chongqing 0.38 35 0.44 25 0.99 40
SWU77 重庆 Chongqing 0.40 26 0.20 45 0.97 49
SWU80 重庆 Chongqing 0.39 28 0.43 28 1.01 29
SWU81 重庆 Chongqing 0.38 39 0.11 60 1.02 27
SWU84 重庆 Chongqing 0.28 114 -0.40 114 0.86 117
SWU88 重庆 Chongqing 0.38 34 -0.07 79 0.97 45
SWU95 重庆 Chongqing 0.26 127 -0.75 136 0.85 119
SWU96 重庆 Chongqing 0.40 22 0.57 19 1.07 15
SWU99 重庆 Chongqing 0.39 31 0.17 49 0.97 43
SWU100 重庆 Chongqing 0.28 117 -0.34 109 0.86 112
SWU110 重庆 Chongqing 0.48 8 1.28 5 1.23 6
SWU111 重庆 Chongqing 0.31 98 -0.30 103 0.87 108
SWU112 重庆 Chongqing 0.37 44 0.10 62 0.98 41
1L187 湖北 Hubei 0.50 6 1.23 6 1.19 8
中双6号Zhongshuang 6 湖北 Hubei 0.41 19 0.83 14 1.07 16
1L191 湖北 Hubei 0.45 10 1.03 10 1.15 9
辐油4号 Fuyou 4 湖北 Hubei 0.32 88 -0.14 89 0.93 71
1L410 湖北 Hubei 0.29 110 -0.12 85 0.88 106
扬J6711 Yang J6711 湖北 Hubei 0.35 62 0.05 67 0.99 36
Monty 湖北 Hubei 0.35 54 0.14 54 0.97 46
史力丰 Shilifeng 湖北 Hubei 0.32 90 -0.15 90 0.91 82
青662A Qing 662A 湖北 Hubei 0.31 97 -0.30 104 0.90 89
WH-19 湖北 Hubei 0.50 7 1.15 8 1.20 7
11-9-704 湖北 Hubei 0.63 2 1.88 2 1.43 1
WH-95 湖北 Hubei 0.34 65 -0.34 108 0.91 83
NY7 湖北 Hubei 0.30 108 -0.15 91 0.88 100
华油6号 Huayou 6 湖北 Hubei 0.29 112 -0.39 113 0.84 122
华油10 Huayou 10 湖北 Hubei 0.39 29 0.32 34 0.96 51
Suigenshu 湖北 Hubei 0.43 14 0.68 18 1.03 24
华油4号 Huayou 4 湖北 Hubei 0.36 53 0.19 47 0.93 68
Y2 江苏 Jiangsu 0.30 106 -0.29 101 0.88 103
勺叶青 Shaoyeqing 江苏 Jiangsu 0.28 121 -0.46 118 0.83 125
沪激早 Lujizao 江苏 Jiangsu 0.32 87 0.14 55 0.94 64
大花球 Dahuaqiu 江苏 Jiangsu 0.33 82 -0.07 81 0.89 95
荣选 Rongxuan 江苏 Jiangsu 0.32 94 -0.21 96 0.90 87
宁油6号 Ningyou 6 江苏 Jiangsu 0.30 99 -0.45 117 0.85 118
广德8104 Guangde 8104 江苏 Jiangsu 0.38 36 0.39 30 1.01 30
宿84-6 Su 84-6 江苏 Jiangsu 0.35 60 0.06 65 0.96 50
芥65-1 Jie 65-1 江苏 Jiangsu 0.34 71 0.14 56 0.93 70
品种(系)
Cultivar (line)
材料来源
Source
ASF值
ASF value
排序
Order
A值
A value
排序
Order
WAC值
WAC value
排序
Order
SWU20 重庆 Chongqing 0.29 111 -0.36 111 0.88 102
SWU28 重庆 Chongqing 0.38 37 0.29 37 0.97 44
SWU40 重庆 Chongqing 0.41 20 0.44 24 1.06 17
SWU47 重庆 Chongqing 0.36 51 0.35 31 0.87 111
SWU48 重庆 Chongqing 0.23 137 -0.74 135 0.78 137
SWU56 重庆 Chongqing 0.23 138 -0.77 137 0.80 131
SWU59 重庆 Chongqing 0.20 143 -0.80 139 0.76 141
SWU65 重庆 Chongqing 0.24 135 -0.71 133 0.80 133
SWU92 重庆 Chongqing 0.28 119 -0.40 115 0.88 105
SWU101 重庆 Chongqing 0.27 126 -0.58 127 0.85 120
川油20 Chuanyou 20 四川 Sichuan 0.38 38 0.26 41 1.04 23
川油18 Chuanyou 18 四川 Sichuan 0.28 116 -0.34 107 0.90 86
CY12Q95406 四川 Sichuan 0.36 47 0.21 43 0.93 69
CY12Q8-7 四川 Sichuan 0.30 104 -0.53 121 0.86 113
CY12QSZ06 四川 Sichuan 0.19 146 -0.99 142 0.76 142
CY12Q95108 四川 Sichuan 0.25 133 -0.58 126 0.82 127
CY12Q21535-N3 四川 Sichuan 0.35 63 0.18 48 0.96 52
wx1025 湖南 Hunan 0.23 139 -0.96 141 0.77 140
wx10315 湖南 Hunan 0.34 69 -0.27 99 0.87 110
10-1358 湖南 Hunan 0.32 85 -0.78 138 0.77 138
湘油13号 Xiangyou 13 湖南 Hunan 0.11 148 -1.54 148 0.57 148
782 湖南 Hunan 0.27 124 -0.57 125 0.80 132
WX10329 湖南 Hunan 0.23 141 -1.06 144 0.76 143
509 湖南 Hunan 0.26 130 -0.66 131 0.80 130
1321 湖南 Hunan 0.30 100 -0.12 86 0.99 39
07022 湖北 Hubei 0.44 12 -0.20 95 1.01 31
07094 湖北 Hubei 0.32 89 -0.05 76 0.89 96
97097 湖北 Hubei 0.39 30 -0.25 98 0.95 57
RQ011 湖北 Hubei 0.40 23 -0.19 94 1.01 28
96021 湖北 Hubei 0.18 147 -1.03 143 0.73 145
01111 湖北 Hubei 0.38 33 -0.05 77 0.99 37
01570 湖北 Hubei 0.44 13 0.74 16 1.04 21
01188 湖北 Hubei 0.64 1 2.02 1 1.39 2
02354 湖北 Hubei 0.34 73 0.00 74 0.92 78
02359 湖北 Hubei 0.33 79 0.30 36 0.88 99
93205 湖北 Hubei 0.27 123 -0.56 124 0.77 139
93210 湖北 Hubei 0.34 68 0.15 53 0.97 48
Nca 湖北 Hubei 0.43 16 0.87 13 1.10 12
中双4号 Zhongshuang 4 湖北 Hubei 0.34 67 0.21 44 0.95 54
中双11号 Zhongshuang 11 湖北 Hubei 0.28 120 -0.51 120 0.83 126
2011-6308 湖北 Hubei 0.37 43 0.32 33 0.95 55
2012-3448 湖北 Hubei 0.23 140 -0.95 140 0.80 129
2012-5086 湖北 Hubei 0.32 86 -0.23 97 0.94 62
2012-8380 湖北 Hubei 0.30 102 -0.18 93 0.89 94
2012-8998 湖北 Hubei 0.40 21 0.51 23 1.00 34
品种(系)
Cultivar (line)
材料来源
Source
ASF值
ASF value
排序
Order
A值
A value
排序
Order
WAC值
WAC value
排序
Order
2012-9478 湖北 Hubei 0.36 48 0.25 42 1.04 20
希望106 Xiwang 106 湖北 Hubei 0.36 49 0.31 35 0.94 65
阳光198 Yangguang 198 湖北 Hubei 0.20 144 -1.19 147 0.71 147
中双10号 Zhongshuang 10 湖北 Hubei 0.35 56 0.16 52 0.92 72
中双12号 Zhongshuang 12 湖北 Hubei 0.36 50 0.44 27 0.99 35
中油589 Zhongyou 589 湖北 Hubei 0.40 24 0.53 21 1.03 25
华油13号 Huayou 13 湖北 Hubei 0.34 74 0.10 61 0.95 56
Rucabo 湖北 Hubei 0.35 55 0.05 68 0.94 66
WH-20 湖北 Hubei 0.37 42 0.26 40 1.04 22
WH-23 湖北 Hubei 0.37 41 0.27 39 1.00 32
WH-24 湖北 Hubei 0.34 77 0.12 59 0.92 76
WH-25 湖北 Hubei 0.33 78 0.00 72 0.94 60
WH-33 湖北 Hubei 0.43 15 0.95 11 1.09 13
WH-37 湖北 Hubei 0.34 66 0.06 66 0.88 101
WH-38 湖北 Hubei 0.30 105 -0.38 112 0.91 79
WH-41 湖北 Hubei 0.25 134 -0.56 123 0.79 135
WH-45 湖北 Hubei 0.31 96 -0.18 92 0.86 116
WH-49 湖北 Hubei 0.28 113 -0.46 119 0.89 93
WH-55 湖北 Hubei 0.51 5 1.13 9 1.23 5
WH-60 湖北 Hubei 0.40 27 0.41 29 1.02 26
甲预17棚 Jiayu 17 peng 湖北 Hubei 0.20 142 -1.15 146 0.72 146
甲预25棚 Jiayu 25 peng 湖北 Hubei 0.30 101 -0.12 87 0.89 92
甲预31棚 Jiayu 31 peng 湖北 Hubei 0.51 3 1.62 4 1.35 3
甲915 Jia 915 湖北 Hubei 0.42 18 0.72 17 1.08 14
甲922 Jia 922 湖北 Hubei 0.20 145 -1.08 145 0.75 144
甲917 Jia 917 湖北 Hubei 0.28 122 -0.36 110 0.88 107
甲931 Jia 931 湖北 Hubei 0.26 131 -0.61 128 0.84 123
甲963棚 Jia 963 peng 湖北 Hubei 0.34 64 0.28 38 0.97 47
沪油17号 Huyou 17 江苏 Jiangsu 0.29 109 -0.10 83 0.95 58
沪油15号Huyou 15 江苏 Jiangsu 0.46 9 1.15 7 1.13 10
宁油14号 Ningyou 14 江苏 Jiangsu 0.33 80 0.00 71 0.92 73
扬油6号 Yangyou 6 江苏 Jiangsu 0.34 72 0.09 63 0.92 77
苏油1号 Suyou 1 江苏 Jiangsu 0.30 107 -0.31 105 0.87 109
浙双8号 Zheshuang 8 江苏 Jiangsu 0.32 91 -0.03 75 0.88 104
浙油758 Zheyou 758 江苏 Jiangsu 0.28 118 -0.42 116 0.84 121
沪油19 Huyou 19 江苏 Jiangsu 0.24 136 -0.55 122 0.79 134
皖油 29 Wanyou 29 江苏 Jiangsu 0.37 40 0.52 22 0.99 38
至尊 Zhizun 青海 Qinghai 0.38 32 0.54 20 0.94 63
A117 陕西 Shaanxi 0.25 132 -0.72 134 0.89 97
B250 陕西 Shaanxi 0.34 76 -0.13 88 0.92 75
B265 陕西 Shaanxi 0.37 45 0.33 32 0.98 42
A109 陕西 Shaanxi 0.51 4 1.66 3 1.32 4
GY284 陕西 Shaanxi 0.30 103 -0.34 106 0.91 80
A97 陕西 Shaanxi 0.33 83 0.00 73 0.94 67
品种(系)
Cultivar (line)
材料来源
Source
ASF值
ASF value
排序
Order
A值
A value
排序
Order
WAC值
WAC value
排序
Order
A148 陕西 Shaanxi 0.36 46 0.17 50 1.04 19
09-P32 湖北 Hubei 0.28 115 -0.29 102 0.86 114
10-P10 湖北 Hubei 0.36 52 0.13 57 0.92 74
11-P30 湖北 Hubei 0.33 81 -0.05 78 0.86 115
03IIB 甘肃 Gansu 0.34 75 0.04 69 0.91 81
DDI 甘肃 Gansu 0.33 84 0.08 64 0.91 84
06T9F 甘肃 Gansu 0.35 61 -0.07 82 0.94 61
03II4B 甘肃 Gansu 0.26 129 -0.62 130 0.84 124
03LF1 甘肃 Gansu 0.26 128 -0.70 132 0.81 128
9852 甘肃 Gansu 0.31 95 -0.28 100 0.91 85
06H7 甘肃 Gansu 0.35 57 0.20 46 0.95 59
CY18PXW-62 四川 Sichuan 0.43 17 0.77 15 1.05 18
96063 湖北 Hubei 0.35 58 0.16 51 0.90 90
浙油21 Zheyou 21 湖北 Hubei 0.32 92 0.02 70 0.96 53
97177 江苏 Jiangsu 0.32 93 -0.11 84 0.88 98

Table 7

Classification of evaluation index of rapeseed germplasm tolerance"

指标
Index
类群 Class
I II III IV
AC值
AC value
发芽势GV 1.533 1.105 1.057 0.850
发芽率GR 1.416 1.088 1.035 0.848
鲜重FW 1.064 0.898 0.811 0.754
干重DW 0.982 0.942 0.985 0.923
根长RL 1.036 0.757 0.657 0.517
芽长SL 1.161 1.140 1.026 0.933
ASF值 ASF value 0.480 0.365 0.320 0.239
WAC值 WAC value 1.178 0.969 0.907 0.786
A值 A value 1.146 0.269 -0.187 -0.788

Fig. 2

Cluster diagram of test germplasm tolerance system based on A value"

Table 8

Grey correlation degree between AC values and A values of various indexes of rape germplasm"

指标
Index
关联度
Correlation degree
权重
Weight
排序
Rank
根长RL 0.313 0.213 1
鲜重FW 0.265 0.180 2
干重DW 0.246 0.168 3
芽长RL 0.218 0.149 4
发芽率GR 0.217 0.148 5
发芽势GV 0.208 0.142 6
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