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Acta Agronomica Sinica ›› 2019, Vol. 45 ›› Issue (10): 1595-1603.doi: 10.3724/SP.J.1006.2019.94024

• RESEARCH NOTES • Previous Articles     Next Articles

DNA methylation involved in regulating drought stress response of potato

LI Peng-Cheng1,2,BI Zhen-Zhen1,2,LIANG Wen-Jun1,2,SUN Chao1,2,ZHANG Jun-Lian1,BAI Jiang-Ping1,2,*()   

  1. 1Gansu Provincial Key Lab of Aridland Crop Science / Gansu Key Lab of Crop Improvement & Germplasm Enhancement, Lanzhou 730070, Gansu, China
    2College of Agronomy, Gansu Agricultural University, Lanzhou 730070, Gansu, China
  • Received:2019-02-16 Accepted:2019-05-12 Online:2019-10-12 Published:2019-09-10
  • Contact: Jiang-Ping BAI E-mail:baijp@gsau.edu.cn
  • Supported by:
    This study was supported by the National Natural Science Foundation of China(31660432);This study was supported by the National Natural Science Foundation of China(31460369);the China Agriculture Research System(CARS-09-P14);the Gansu Potato Industry System (GARS-03-P1)(GARS-03-P1);the “Light of the West” Talent Training Program of the Chinese Academy of Sciences(2014-01);the Lanzhou Science and Technology Development Plan(2015-3-62);the Gansu Science and Technology Fund(18JR3RA170);the Gansu Provincial Department of Education University Research Project(2018A-040)

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Abstract:

Although epigenetics is essential for regulating gene expression in plant under abiotic stress, there are few reports regarding the epigenetics of potato under drought stress. In this study, five potato cultivars including Atlantic, Favorita, C119, C16, and Qingshu 9, were used to verify that DNA methylation is involved in potato drought stress response. The plantlets were cultured for 24 days on MS medium, MS medium supplemented with 60 μmol L -1 methylation inhibitor (5-azadC), 200 mmol L -1 mannitol, and 60 μmol L -1 methylation inhibitor combined with 200 mmol L -1 mannitol, respectively. Phenotypes, and physiological and biochemical traits were recorded and comprehensively analyzed. The response of five potato cultivars to mannitol and 5-azadC was similar. With treatments of mannitol or 5-azadC, the dry/fresh weight, shoot height, leaf number and chlorophyll content decreased significantly (P < 0.05), while the activities of SOD, POD, CAT, and contents of proline and MDA increased significantly (P < 0.05). No significant changes were observed in branch number, root length and average root diameter, indicating that potato traits might have different regulatory pathways in response to drought stress and DNA demethylation. Compared with the individual treatment, combined-treatment further inhibited the growth of plantlets, and increased activities of SOD, POD, CAT, and contents of MDA and Pro under drought stress and 5-azadC treatment, indicating that the formation of some phenotypes (not all) in response to drought was regulated through the DNA methylation. The results provide preliminary data for further study on epigenetics regulatory pathway of potato under drought stress.

Key words: potato, drought stress, DNA methylation, morphological physiology, comprehensive analysis

Fig. 1

Image of potato plantlets in vitro under different treatments A-E are the phenotype of Atl, FAV, C119, C16, and QS9 after 24 days of treatment. T0: CK; T1: 5-azadC; T2: mannitol; T3: 5-azadC+mannitol. Three plantlets of each treatment were selected for photography. Bar = 2 cm."

Table 1

Response of potato varieties to mannitol and 5-azadC"

指标
Index		 大西洋
Atlantic		 费乌瑞它Favorita C119 C16 青薯9号
Qingshu 9		 平均值
Mean
抗旱系数 Coefficients of resistance to drought
鲜重 FW 0.3685 0.3872 0.2977 0.2954 0.7387 0.4175
干重 DW 0.4218 0.6099 0.3273 0.3579 0.7604 0.4955
株高 SH 0.3537 0.4247 0.5353 0.5650 0.2972 0.4352
分枝数 NB 0.2500 0.5455 0.2545 0.4167 0.8889 0.4711
叶片数 NL 0.3421 0.3953 0.3978 0.4359 0.9615 0.5065
总根长 RL 0.7010 0.8807 0.5532 0.7902 0.7817 0.7414
平均根粗 ARD 0.9785 1.0468 0.9003 0.8730 0.9584 0.9514
超氧化物歧化酶 SOD 1.2969 1.2707 1.2415 1.4528 1.8126 1.4149
过氧化物酶 POD 1.2224 1.2177 1.4230 1.4113 2.2462 1.5041
过氧化氢酶 CAT 1.6252 1.7085 1.8966 1.7844 3.5571 2.1144
游离脯氨酸 Pro 1.2920 1.3428 1.6898 1.9928 2.3975 1.7430
丙二醛 MDA 2.9195 2.3183 2.8036 2.1572 1.7162 2.3830
叶绿素 Chl 0.5980 0.8280 0.5491 1.0459 0.9492 0.7940
综合响应系数CRC 0.9515 0.9982 0.9900 1.0445 1.3897 1.0748
指标
Index		 大西洋
Atlantic		 费乌瑞它Favorita C119 C16 青薯9号
Qingshu 9		 平均值
Mean
5-azadC响应系数 Coefficients of response to 5-azadC
鲜重 FW 0.7594 0.7300 0.4364 0.6180 0.3968 0.5881
干重 DW 0.5741 0.7590 0.4719 0.6882 0.4547 0.5896
株高 SH 0.5488 0.5679 0.5620 0.6179 0.6226 0.5839
分枝数 NB 0.5000 0.9091 0.5455 0.8333 0.4444 0.6465
叶片数 NL 0.8333 0.6357 0.6935 0.8462 0.8077 0.7633
总根长 RL 0.8466 0.6714 0.6726 0.8118 0.8412 0.7687
平均根粗 ARD 0.9325 1.1128 0.9718 0.9821 0.9572 0.9913
超氧化物歧化酶 SOD 1.1333 1.1594 1.1133 1.1076 1.5522 1.2131
过氧化物酶 POD 1.0898 1.0486 1.3302 1.1552 1.7649 1.2777
过氧化氢酶 CAT 1.5630 1.5851 1.5766 1.3145 2.1498 1.6378
游离脯氨酸 Pro 1.0258 1.0236 1.2417 1.7473 2.1548 1.4386
丙二醛 MDA 1.1743 1.0931 1.0387 1.1621 0.9300 1.0797
叶绿素 Chl 1.2042 1.7738 0.7249 1.3946 1.3714 1.2938
综合响应系数CRC 0.9373 1.0053 0.8753 1.0214 1.1114 0.9902

Fig. 2

Changes in phenotypes and physiological and biochemical indices of potato plantlets in vitro under different treatments Multiple comparisons were performed with Duncan’s method (P < 0.05)."

Table 2

Eigenvalue and contribution rate of each comprehensive index"

主成分
Principal component		 抗旱性 Drought resistance 5-azadC响应性 Response to 5-azadC
PC1 PC2 PC3 PC1 PC2 PC3
特征值 Eigenvalue 8.595 2.460 1.719 6.750 2.757 2.456
贡献率 Contribution rate (%) 66.12 18.92 13.22 51.92 21.20 18.89
累计贡献率 Cumulative contribution rate (%) 66.12 85.04 98.26 51.92 73.12 92.01

Table 3

Matrix of phenotypic factor loading"

主成分
Principal component		 抗旱性 Drought resistance 5-azadC响应性 Response to 5-azadC
PC1 PC2 PC3 PC1 PC2 PC3
鲜重 FW 0.322 -0.105 0.214 -0.316 -0.114 0.207
干重 DW 0.294 -0.313 0.026 -0.322 0.185 0.288
株高 SH -0.205 0.377 -0.341 0.217 0.201 0.400
分枝数 NB 0.331 -0.092 -0.093 -0.294 0.279 0.210
叶片数 NL 0.332 0.086 0.145 0.159 -0.383 0.415
总根长 RL 0.148 -0.343 -0.546 0.187 -0.291 0.434
平均根粗 ARD 0.039 -0.627 0.022 -0.233 0.479 -0.023
超氧化物歧化酶 SOD 0.327 0.137 0.018 0.327 0.237 0.112
过氧化物酶 POD 0.315 0.179 0.200 0.374 0.138 -0.039
过氧化氢酶 CAT 0.322 0.095 0.221 0.312 0.221 -0.069
游离脯氨酸 Pro 0.273 0.382 -0.027 0.311 0.123 0.290
丙二醛 MDA -0.309 -0.068 0.304 -0.304 -0.319 0.198
叶绿素 Chl 0.214 0.111 -0.571 -0.123 0.364 0.409

Table 4

Comprehensive index (CI), index weight, μ(X), and comprehensive evaluation value (D) of varieties"

品种
Variety		 CI(1) CI(2) CI(3) μ(X1) μ(X2) μ(X3) 综合评价值
Comprehensive
assessment value (D)		 综合排名
Order
抗旱性Drought resistance
大西洋 Atlantic -1.9419 -1.0192 0.9505 0.0421 0.2893 0.9236 0.2083 4
费乌瑞它 Favorita -0.4128 -2.0934 -1.0274 0.2524 0.0000 0.2496 0.2035 5
C119 -2.2478 1.3289 1.1746 0.0000 0.9216 1.0000 0.3120 3
C16 -0.4186 1.6201 -1.7600 0.2516 1.0000 0.0000 0.3619 2
青薯9号 Qingshu 9 5.0211 0.1637 0.6623 1.0000 0.6078 0.8254 0.9010 1
权重 Weight 0.6729 0.1926 0.1345
5-azadC响应性Response to 5-azadC
大西洋 Atlantic -0.8022 -2.2132 0.1744 0.2937 0.0000 0.6020 0.2893 5
费乌瑞它 Favorita -2.8667 2.1615 -0.1491 0.0000 1.0000 0.5272 0.3387 4
C119 0.3390 -0.4624 -2.4315 0.4560 0.4002 0.0000 0.3495 3
C16 -0.8330 -0.4878 1.8975 0.2893 0.3944 1.0000 0.4594 2
青薯9号 Qingshu 9 4.1629 1.0020 0.5086 1.0000 0.7350 0.6792 0.8731 1
权重 Weight 0.5643 0.2304 0.2053

Table 5

Comparative analysis of phenotypes of plantlets in vitro under the treatments of Mannitol and 5-azadC (alone and in combination)"

指标
Index		 平均值 Mean 变幅 Range
T1/T0 T2/T0 T3/T0 T2/T0 vs. T1/T0 (%) T3/T0 vs. T1/T0 (%) T3/T0 vs. T2/T0 (%)
鲜重 FW 0.417 b 0.589 a 0.316 c -46.40 -29.19 -24.30
干重 DW 0.498 b 0.589 a 0.381 c -35.25 -15.46 -23.41
株高 SH 0.435 b 0.585 a 0.346 c -40.83 -25.54 -20.54
分枝数 NB 0.510 ab 0.697 a 0.339 b -51.37 -26.85 -33.52
叶片数 NL 0.508 b 0.764 a 0.364 c -52.41 -33.55 -28.39
总根长 RL 0.741 a 0.773 a 0.653 b -15.43 -4.05 -11.86
平均根粗 ARD 0.958 a 0.996 a 0.921 a -7.56 -3.84 -3.87
超氧化物歧化酶 SOD 1.426 b 1.221 c 1.656 a 35.63 16.79 16.13
过氧化物酶 POD 1.521 b 1.284 c 1.675 a 30.45 18.46 10.12
过氧化氢酶 CAT 2.190 a 1.691 b 2.570 a 51.98 29.51 17.35
游离脯氨酸 Pro 1.752 a 1.463 b 1.825 a 24.74 19.75 4.17
丙二醛 MDA 2.409 a 1.090 b 2.614 a 139.82 121.01 8.51
叶绿素 Chl 0.795 c 1.262 a 1.165 b -7.69 -37.00 46.54
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