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Acta Agronomica Sinica ›› 2019, Vol. 45 ›› Issue (11): 1764-1769.doi: 10.3724/SP.J.1006.2019.82066

• RESEARCH NOTES • Previous Articles    

Pyramiding and evaluation of brown planthopper resistance genes in water-saving and drought-resistance restorer line

ZHANG An-Ning1,2,LIU Yi1,2,WANG Fei-Ming1,XIE Yue-Wen2,KONG De-Yan1,NIE Yuan-Yuan3,ZHANG Fen-Yun1,BI Jun-Guo1,YU Xin-Qiao1,LIU Guo-Lan1,LUO Li-Jun1,2,*()   

  1. 1 Shanghai Agrobiological Gene Center, Shanghai 201106, China
    2 College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, China
    3 Jiangxi Super Rice Research and Development Center, Nanchang 330200, Jiangxi, China
  • Received:2018-12-20 Accepted:2019-06-12 Online:2019-11-12 Published:2019-07-09
  • Contact: Li-Jun LUO E-mail:lijun@sagc.org.cn
  • Supported by:
    This study was supported by the Shanghai Seed Industry Development Project(2019-02-08-00-08-F01110);the Shanghai Agriculture Applied Technology Development Program(2018-02-08-00-08-F01553);the National Key Research and Development Program of China(2017YFD0100304)

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Abstract:

The brown planthopper (Nilaparvata lugens St?l, BPH) is the most serious pest threat to rice production across Asia. Increasing host-plant resistance is the most economical and ecological strategy for controlling this pest. The objective of this study was to survey the resistance effects of different R genes to brown planthopper, reveal its influence on agronomic traits, and provide insights into molecular breeding of rice with resistance to brown planthopper. In this research, the brown planthopper R genes Bph6, Bph9, Bph14, and Bph15 were introgressed into the water-saving and drought-resistant rice restorer line ‘Hanhui 3’ through marker-assisted backcrossing scheme. The standard seedling group screening method was used to identify the resistance of the improved lines. Among single-gene improved lines, the order of the R genes effects was Bph9 > Bph6 > Bph15 > Bph14, and among pyramiding improved lines, that was Bph6+Bph9+Bph14+Bph15 > Bph6+Bph9 > Bph6+Bph9+Bph14 > Bph6+Bph9+Bph15 > Bph6+Bph14+Bph15 > Bph9+Bph14+Bph15 > Bph14+Bph15. Furthermore, the survey of agronomic traits demonstrated that there were no significant differences between the 11 improved lines and recurrent parent ‘Hanhui 3’ in plant height, panicle number per plant and 1000-grain weight. These results suggest that the introgression of Bph6, Bph9, Bph14, and Bph15 genes by molecular marker-assisted selection technology could enhance the resistance to brown planthopper and improve breeding efficiency.

Key words: breeding, brown planthopper, resistance gene, marker-assisted selection, resistance evaluation, water-saving and drought-resistance rice

Fig. 1

Breeding restorer lines with resistance to brown planthopper"

Fig. 2

PCR analysis for brown planthopper resistance genes A: Bph6-linked marker RM5757; B: Bph9-linked marker InD28432; C: Bph14-linked marker 76-2; D: Bph15-linked marker MS5. M: D2000 marker; P1: recurrent parent Hanhui 3; P2: donor parent; 1-11: improved lines 18R1-18R11."

Table 1

Evaluation for brown planthopper resistance at seedling stage"

编号
No.		 鉴定编号
Identification no.		 基因型
Genotype		 抗性表现
Resistance performance		 抗性级别
Resistance level#
18R1 18FS30 Bph6 2.60±0.81 抗虫 R
18R2 18FS72 Bph9 2.40±0.35 抗虫 R
18R3 18FS32 Bph14 5.20±0.76 中抗 MR
18R4 18FS71 Bph15 4.30±0.97 中抗 MR
18R5 18FS86 Bph6+Bph9 1.10±0.21 高抗 HR
18R6 18FS83 Bph14+Bph15 1.93±0.41 高抗 HR
18R7 18FS90 Bph6+Bph9+Bph14 1.27±0.27 高抗 HR
18R8 18FS88 Bph6+Bph9+Bph15 1.50±0.38 高抗 HR
18R9 18FS92 Bph6+Bph14+Bph15 1.60±0.31 高抗 HR
18R10 18FS94 Bph9+Bph14+Bph15 1.70±0.35 高抗 HR
18R11 18FS97 Bph6+Bph9+Bph14+Bph15 0.53±0.24 高抗 HR
旱恢3号 Hanhui 3 18FS26 8.93±0.07 高感 HS
TN1 8.80±0.12 高感 HS

Table 2

Agronomic traits of improved lines"

编号
No.		 基因型
Genotype		 株高
Plant height (cm)		 单株有效穗
Panicles
per plant		 穗长
Panicle length
(cm)		 每穗总粒数
Grains per
panicle		 结实率
Grain fertility
(%)		 千粒重
1000-Grain weight (g)		 单株产量
Grain yield
per plant (g)
18R1 Bph6 121.00±1.00 13.67±3.21 21.49±0.58 200.30±2.84** 83.35±3.04 26.35±0.88 32.34±0.70*
18R2 Bph9 119.68±1.64 9.88±0.55 25.53±0.87** 202.65±5.16** 77.62±1.78 24.79±1.66 33.94±1.74
18R3 Bph14 129.00±6.24 10.00±4.00 24.88±1.80 199.98±7.81** 90.37±1.14 25.00±1.20 29.04±0.73**
18R4 Bph15 120.48±1.36 10.70±1.00 26.13±0.45** 200.22±3.20** 79.16±2.39 24.32±0.54 34.94±1.47
18R5 Bph6+Bph9 120.65±2.67 10.58±0.64 25.75±0.57** 201.66±2.29** 76.68±0.98* 24.44±1.58 31.99±3.46*
18R6 Bph14+Bph15 119.71±2.21 9.88±0.64 26.08±0.49** 198.87±7.43** 80.37±1.66 26.07±1.82 30.64±1.71**
18R7 Bph6+Bph9+Bph14 118.12±1.46 10.50±1.04 24.90±0.48* 194.34±2.80** 71.64±1.30** 26.21±1.12 34.90±0.71
18R8 Bph6+Bph9+Bph15 124.19±0.13 10.04±0.69 25.13±0.85* 205.54±5.21** 71.86±1.73** 24.44±0.88 32.75±2.89
18R9 Bph6+Bph14+Bph15 119.89±1.59 10.95±0.23 24.94±0.61* 200.59±4.13** 68.69±1.13** 24.87±1.73 36.47±1.04
18R10 Bph9+Bph14+Bph15 118.41±3.68 10.99±0.53 26.14±0.62** 201.56±2.44** 69.90±3.57** 26.53±0.52 31.19±1.82*
18R11 Bph6+Bph9+Bph14+Bph15 121.12±2.63 11.33±0.56 25.82±0.36** 200.79±6.06** 57.26±2.73** 26.40±1.21 30.38±1.89**
Hanhui 3 117.00±4.90 10.00±0.82 22.80±0.41 240.69±21.35 86.24±6.49 25.84±1.62 42.11±6.78
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