小麦株高QTL Qph.nau-5B的效应评价

doi:10.3724/SP.J.1006.2021.01053

Abstract

Abstract:

Plant height affects directly the yield potential of wheat, and constitutes an important component of plant resistance to lodging. Although a large number of QTLs for plant height were detected, most of them have not been evaluated for their genetic effects yet. In the previous study, a major QTL Qph.nau-5B controlling wheat plant height was identified through association mapping in wheat variety collection. To evaluate the dwarfing effect of this QTL, three near-isogenic lines (NILs) with different alleles of Qph.nau-5B were developed using marker-assisted selection with Nanda 2419, Jichun 1016, and Zhengmai 9023 as donor and Zhongyou 9507 as receptor. The recipient genome compositions of these NILs were higher than 93%. Seven independent field trials were conducted and revealed that, compared with the recurrent parent, all NILs indicated a significant decrease in plant height (11.1 cm or 10.3% on average). Three alleles of Qph.nau-5B showed different degrees of dwarfing effects. The alleles coming from Jichun 1016 and Zhengmai 9023 displayed a similar effect on plant height (12.4 cm) in all environments, stronger than that of Nanda 2419 (8.6 cm). However, the relative dwarfism effects of different alleles were affected by different environments. Further analysis elucidated that this QTL had little detrimental influence on other agronomical traits such as spike number per plant, spike length and 1000-grain weight. These results suggested the breeding value of Qph.nau-5B that would be utilized for molecular design breeding of plant architecture in wheat.

Key words: wheat (Triticum aestivum), plant height, QTL, Qph.nau-5B, near-isogenic lines (NILs), marker-assisted selection

HAN Yu-Zhou, ZHANG Yong, YANG Yang, GU Zheng-Zhong, WU Ke, XIE Quan, KONG Zhong-Xin, JIA Hai-Yan, MA Zheng-Qiang. Effect evaluation of QTL Qph.nau-5B controlling plant height in wheat[J].Acta Agronomica Sinica, 2021, 47(6): 1188-1196.

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References 59

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近等基因系 NILs	供体亲本 Donor parents	轮回亲本 Recurrent parent	世代 Generation	背景回复率 Recipient genome composition (%)
NIL-ND	南大2419 Nanda 2419	中优9507 Zhongyou 9507	BC₄F₂	96.8
NIL-JC	吉春1016 Jichun 1016	中优9507 Zhongyou 9507	BC₄F₂	93.3
NIL-ZM	郑麦9023 Zhengmai 9023	中优9507 Zhongyou 9507	BC₄F₂	98.4

变异因子 Variation factors	F值 F-value	P值 P-value
基因型 Genotype	113.95	<0.0001
环境 Environment	218.02	<0.0001
基因型 × 环境 Genotype × Environment	5.58	<0.0001

株系Line	2017HA	2018HA	2019HA	2018JP	2019LH	2017TA	2018TA
中优9507 Zhongyou 9507	9.2±0.5	6.3±0.7	6.8±0.3	6.3±0.4	6.9±0.4	22.9±1.0	14.6±0.1
NIL-ND	8.7±0.3	6.0±0.7	7.8±2.4	5.2±0.4	—	21.4±0.9	15.6±1.3
NIL-JC	9.0±0.5	5.8±0.4	—	6.2±0.1	5.8±0.1	—	14.8±0.4
NIL-ZM	8.1±0.4	5.7±1.5	7.4±0.7	6.3±0.4	6.6±0.0	19.5±0.8^*	15.6±0.4

株系Line	2017HA	2018HA	2019HA	2018JP	2019LH	2017TA	2018TA
中优9507 Zhongyou 9507	9.7±0.1	9.1±0.1	10.5±0.1	9.7±0.1	10.8±0.2	11.3±0.2	9.9±0.1
NIL-ND	9.8±0.1	9.1±0.1	10.8±0.1	9.9±0.5	10.8±0.0	11.6±0.2	9.6±0.3
NIL-JC	10.3±0.2^**	10.7±0.1^**	—	10.4±0.9	11.9±0.3^*	—	10.0±0.3
NIL-ZM	9.4±0.2	—	10.8±0.4	9.6±0.2	10.8±0.5	11.7±0.2	9.9±0.3

株系Line	2017HA	2018HA	2019HA	2018JP	2019LH	2017TA	2018TA
中优9507 Zhongyou 9507	52.4±0.4	51.7±1.1	49.2±0.0	51.4±3.0	51.1±0.6	59.1±0.8	53.7±1.1
NIL-ND	51.9±0.4	50.4±0.9	49.1±4.8	52.0±3.0	50.6±1.3	57.1±0.7	50.6±0.4^*
NIL-JC	—	53.3±0.8	52.8±1.7^*	54.6±1.7	53.9±0.4	54.4±0.9^*	49.1±2.1^**
NIL-ZM	52.9±0.8	54.8±1.7^*	50.6±1.1	53.7±1.4	52.6±0.6	56.9±0.4	51.4±1.2

Effect evaluation of QTL Qph.nau-5B controlling plant height in wheat

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[8]	WANG Ying, GAO Fang, LIU Zhao-Xin, ZHAO Ji-Hao, LAI Hua-Jiang, PAN Xiao-Yi, BI Chen, LI Xiang-Dong, YANG Dong-Qing. Identification of gene co-expression modules of peanut main stem growth by WGCNA [J]. Acta Agronomica Sinica, 2021, 47(9): 1639-1653.
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