基于SSR标记的雪茄烟种质资源指纹图谱库的构建及遗传多样性分析

doi:10.3724/SP.J.1006.2021.04183

Abstract

Abstract:

In order to investigate the genetic diversity of Chinese cigar germplasm resources in molecular level and to establish the DNA fingerprint database of cigar varieties, 43 pairs of SSR primers with good polymorphic were used to analyze the genetic diversity of 220 cigar germplasms, and 14 pairs of core primers were screened from 43 pairs of SSR primers to construct the fingerprint database of 220 cigar germplasms in the study. Results showed that a total of 243 alleles were amplified by 43 pairs of SSR primers in 220 cigar germplasm materials, with an average of 5.65 per marker, ranging from 2 to 13. The polymorphic information content (PIC) of each locus varied from 0.2078 to 0.9087 with an average of 0.6360. The number of effective alleles (N_e) ranged from 1.3081 to 11.7876, and the average number of effective alleles was 3.9077. The observed heterozygosity (H_o) ranges from 0.0828 to 0.7639 with an average of 0.3191. The expected heterozygosity (H_e) ranged from 0.2361 to 0.9172 with an average of 0.6809. The average Shannon genetic diversity index (I) was 1.3756, the genetic distance was between 0.0233 and 0.9286, and the average genetic distance was 0.6816. Unweighted pair-group method with arithmetic means (UPGMA) cluster analysis showed that the tested cigar resources can be divided into three groups at the genetic distance of 0.74. All tested materials were divided into two groups by population genetic structure analysis and principal component analysis (PCA). Based on the primer analysis and phenotypic identification, it was determined that the ‘LZ’, ‘FS’ and ‘MED’, ‘SDDY’ and ‘MDJ 05-1’, ‘Florida 513’ and ‘CA0701’ were the same species with different names. One variety retained one germplasm, leaving 216 different germplasm. 14 pairs of SSR primers which could distinguish all the tested materials were screened to construct the fingerprint database of 216 cigar cultivars from 43 pairs of SSR primers as core primers. In conclusion, cigar germplasm resources in China had a high level of genetic diversity. In this study, the constructed SSR fingerprint database and genetic analysis of cigar germplasm resources provided the scientific basis for the screening and identification of high-quality cigar germplasms resources, the mining of important genes, and the broadening the basis of cigar genetic breeding.

Key words: cigar, germplasm resources, SSR marker, genetic diversity, fingerprint database

WANG Yan-Yan, WANG Jun, LIU Guo-Xiang, ZHONG Qiu, ZHANG Hua-Shu, LUO Zheng-Zhen, CHEN Zhi-Hua, DAI Pei-Gang, TONG Ying, LI Yuan, JIANG Xun, ZHANG Xing-Wei, YANG Ai-Guo. Construction of SSR fingerprint database and genetic diversity analysis of cigar germplasm resources[J].Acta Agronomica Sinica, 2021, 47(7): 1259-1274.

Figures/Tables 9

Table 1

Information of SSR markers"

引物 Primer	上游引物 Forward primer (5'-3')	下游引物 Reverse primer (5'-3')	基因组 Group	位置 Position
PT52940	TCTTAGCCGTCCATCTCTGC	CACAGGACAATTTGTTGCAGTT	7	134.635
PT52118	ATCCCAAGCAAGTTGGTGTC	AACGAGCATTATGGGATTGC	18	70.312
PT51894	AACAACAGCGGATCACATTG	TGTATGCAACCCACAGAACAA	8	78.215
PT30163	CAACACACACGCTCATCAGA	TCCTCATCCATCGGTAAAGG	5	12.216
PT50433	TTTGTAAATTGTGTGTATAGAGGAGAG	GCACTGGATCTCCTGCATTT	16	160.451
PT51005	CCCTTCGTTCTACGAACCCT	GGGTGCCACCTCACTTTCT	10	55.231
PT51145	ATGCAAATGCAAGATGGTCA	CCGTTCAACTTGGCTTTACC	3	103.357
PT51234	AATTGGACCCAACCACTCTG	TAGCAGGGAAGCCGAAAGTA	19	66.109
PT51289	GAGTTGTGGCCAAGTAGCCT	GGCTTTAGCCAAACGCTCTA	21	49.700
PT51420	CGCCATTACGAACACTAGCA	AAGTTTAGTCCGGTCCGGTT	16	73.595
PT52821	GGGCAATGGAGCAATAGAAA	TGCTTAAAGATTTCCACTCGTT	20	95.174
PT53857	TTCATAAATCAACCTTAAGTGGAGC	TTTGGAGCTTGAAAGCAAAGA	22	67.708
PT54027	GGATCTGCTCATTTCCTATGG	CAAGGCAGCTAAGCCTAACAA	11	50.950
PT54444	GTGCCCATACCAGATTGTGA	ATTCTTCCAACATTCGCACC	6	54.463
PT55056	GCTCTTTAGTCTTTAGGGAGTTGG	TGAATTGAGCTCCTTCCAGG	14	0.276
PT60037	TGGTCTTTGTTCACCACCAA	GCTGGTTCCATCAATTCCAT	4	88.053
PT60146	AAACCTGGAAAGCAACATCA	GACCACGTTGTGTAGTTGTATTCTT	15	45.400
PT60379	AATTTGCACTCTGCGGACTC	CCCTGGGAGTTTCCATTTCT	14	28.140
PT60726	GCAGAGGCAACAATGCATAC	AGCTCGTTGCTGTTTCATCA	20	2.521
PT60990	TCAACCCATAAAGCTGCTCC	AAAGAGACAAAGCAGGCACAA	23	4.978
PT61187	GCTCAGTCGTGAAGAAACAGAA	AGGGAATCCTTGGTTGGTTT	5	130.242
PT61220	TTGGCATGGTTGTGTAAGGA	GCCACAAGTTATAATTTAAAGCTACCA	6	102.936
PT61226	CCTTGGACTCACAATACCATCA	CCTGTTAGCCAAAGGGTAATTC	13	46.719
PT30272	GAACCTAACCTCGCTCCACA	AAATGGTAGCTGCGAGGAGA	15	106.325
PT60427	TGATTCCCTAACCTGCGAAC	CCATTCTTGAGCTTCGTTCTTT	21	5.867
PT51378	ATGTCGGGAAGATTCCACAG	ACAACCAGCTAAATGTGCCC	17	111.136
PT1245	AGTTTAATGTGGGAGCGA	TCGGTCGGTATTAGTGGT	11	39.717
PT50736	CCAAGTAGCGACGAGGAGAA	TTATCCACGCGTCACCATTA	24	53.932
PT51059	CAAACCAGCGCATCTGTCTA	CACCAGAACCCGCTAGTCTC	18	15.309
PT51144	ACCGACAACACACGATTGTA	GCTTGTCGCTTAGCTTAACCAT	10	24.533
PT51878	TGGGAAGAGTGCTACCTTAGTACA	GCGATGTACTCCTAAAGATGTCAA	24	60.520
PT51976	CAGAGGGTAGTGAAGTATTCAATTT	GTTATCAATGCCCTGCCCT	22	94.306
PT52088	TGTGATTCGAACTTGTGATCCT	CATCAGTGGAAAGCGACTCA	7	87.079
PT52156	TGAGAATTCATATAACCGACCCA	TGCCTTCTTTCATACTGCCA	23	60.339
PT52176	TTGCATAAGAGTACCCATCTCG	TGTGTGTGTGAAGCTTGAAGAA	9	120.275
PT52689	GCACCAGGTGAAGTAAAGACAA	TGAAATGCATGTAAAGGAAAGG	13	66.991
PT53216	TCCAAACTCTTATCCCTGATCC	CACCTACCCTGTCGCTGTTT	2	0
PT54394	CATCTCAACTCGCCTGACAA	GCGGCAGGGTTCTATGATTA	8	96.735
PT54889	GGGTCTAAATAATCGCAATTGAA	CGCAGGTGGACTTAAAGAATC	19	106.147
PT60080	CTACGCAGAATCCAATTCCA	CATTAGGCCATAGCCATCCA	3	179.150
PT60917	CAGGAGACTCCATAAATTCACTCTT	AGCATGGTTGCTATTGGCTG	9	38.417
PT61025	GAACCAATCCAAGAGCCAGA	TCAGCCAAATATGTAGCAGGTG	1	87.176
PT61343	ACCCATTTAATTGCCACGTC	TATTCCGGCAGGAGTTTGAC	1	22.254

Table 1

Table 2

Genetic diversity of 43 SSR makers in 220 cigar germplasm resources"

引物 Primer	等位基因数 Number of alleles (N_a)	有效等位基因数 Number of effective alleles (N_e)	Shannon’s 多样性指数 Shannon’s diversity index (I)	观测杂合度Observed heterozygosity (H_o)	期望杂合度Expected heterozygosity (H_e)	Nei’s 多样性指数 Nei’s diversity index (H)	多态性信息含量 Polymorphism information content (PIC)
PT52940	6	3.6152	1.3638	0.2750	0.7250	0.7234	0.6729
PT52118	4	2.9357	1.2000	0.3391	0.6609	0.6594	0.6019
PT51894	4	3.7568	1.3501	0.2645	0.7355	0.7338	0.6838
PT30163	4	2.2131	1.0189	0.4506	0.5494	0.5481	0.5017
PT50433	5	2.6611	1.0961	0.3743	0.6257	0.6242	0.5495
PT51005	7	5.0382	1.7676	0.1966	0.8034	0.8015	0.7760
PT51145	4	3.4657	1.3172	0.2869	0.7131	0.7115	0.6634
PT51234	4	1.5812	0.7298	0.6316	0.3684	0.3676	0.3415
PT51289	4	3.7381	1.3521	0.2658	0.7342	0.7325	0.6838
PT51420	4	2.9343	1.1847	0.3393	0.6607	0.6592	0.5943
PT52821	6	3.4023	1.3790	0.2923	0.7077	0.7061	0.6543
PT53857	6	1.8837	0.8822	0.5298	0.4702	0.4691	0.4117
PT54027	8	4.2139	1.6993	0.2355	0.7645	0.7627	0.7364
PT54444	3	2.4175	0.9805	0.4123	0.5877	0.5864	0.5170
PT55056	4	2.5613	1.1245	0.3890	0.6110	0.6096	0.5573
PT60037	5	2.4156	1.0418	0.4126	0.5874	0.5860	0.5153
PT60146	6	3.7451	1.4714	0.2653	0.7347	0.7330	0.6907
PT60379	6	4.4270	1.5781	0.2240	0.7760	0.7741	0.7375
PT60726	4	2.7073	1.1729	0.3679	0.6321	0.6306	0.5829
PT60990	5	4.3975	1.5329	0.2255	0.7745	0.7726	0.7352
PT61187	7	6.0233	1.8663	0.1641	0.8359	0.8340	0.8126
PT61220	6	4.8084	1.6639	0.2061	0.7939	0.7920	0.7621
PT61226	9	5.9444	1.9354	0.1663	0.8337	0.8318	0.8108
PT30272	7	5.6551	1.8289	0.1749	0.8251	0.8232	0.7999
PT60427	6	3.7898	1.5358	0.2622	0.7378	0.7361	0.7020
PT51378	7	5.5863	1.8098	0.1771	0.8229	0.8210	0.7969
PT1245	4	2.6871	1.0545	0.3707	0.6293	0.6279	0.5518
PT50736	3	2.1267	0.8116	0.4690	0.5310	0.5298	0.4192
PT51059	6	1.4182	0.6834	0.7045	0.2955	0.2949	0.2854
PT51144	8	5.2021	1.7598	0.1904	0.8096	0.8078	0.7804
PT51878	4	3.8172	1.3605	0.2603	0.7397	0.7380	0.6891
PT51976	9	6.7316	1.9878	0.1466	0.8534	0.8514	0.8335
PT52088	3	1.7058	0.7370	0.5853	0.4147	0.4138	0.3724
PT52156	4	2.0324	0.7439	0.4909	0.5091	0.5080	0.3878
PT52176	2	1.3081	0.3983	0.7639	0.2361	0.2355	0.2078
PT52689	13	11.7876	2.5112	0.0828	0.9172	0.9152	0.9087
PT53216	5	4.7386	1.5826	0.2092	0.7908	0.7890	0.7553
PT54394	4	2.1517	0.9554	0.4635	0.5365	0.5352	0.4776
PT54889	7	5.8341	1.8356	0.1695	0.8305	0.8286	0.8054
PT60080	10	7.0791	2.0931	0.1393	0.8607	0.8587	0.8433
PT60917	9	6.4282	1.9900	0.1536	0.8464	0.8444	0.8264
PT61025	5	2.7206	1.1786	0.3661	0.6339	0.6324	0.5793
PT61343	6	4.3419	1.5823	0.2285	0.7715	0.7697	0.7340
平均值Mean	5.6512	3.9077	1.3756	0.3191	0.6809	0.6793	0.6360

Table 2

Fig. 1

Fig. 2

Fig. 3

Fig. 4

Table 3

SSR fingerprinting of 216 cigar germplasm resources"

编号 No.	SSR指纹图谱 SSR fingerprinting	编号 No.	SSR指纹图谱 SSR fingerprinting
1	A7B7C4D1E3F7G2H2I2J3K3L3M3N2	110	A9B9C3D6E3F4G3H2I3J4K3L4M3N4
2	A7B7C4D1E3F7G2H3I2J3K3L3M3N2	111	A8B6C4D6E4F0G3H0I3J3K3L4M3N2
3	A10B2C7D4E4F8G5H2I1J1K1L3M1N3	112	A9B5C7D5E0F0G3H0I3J3K3L2M3N2
4	A10B2C6D4E4F8G5H4I1J1K1L3M1N3	113	A9B5C7D4E3F4G3H2I3J3K3L2M3N2
5	A2B4C5D4E2F8G5H4I4J1K2L2M1N3	114	A12B9C4D9E5F3G4H3I2J3K3L3M1N2
6	A2B4C5D4E2F8G5H4I4J1K2L3M1N3	115	A8B8C5D8E1F0G3H0I2J3K3L3M3N2
7	A8B7C5D4E4F2G2H4I3J4K2L4M3N1	116	A9B7C4D8E1F3G3H1I2J3K2L4M3N4
8	A8B2C5D4E7F2G2H1I3J4K2L3M2N1	117	A2B4C7D3E3F7G5H4I1J1K1L4M1N3
9	A1B7C3D4E7F5G3H2I4J4K2L4M2N1	118	A11B2C5D6E6F4G4H0I3J3K2L4M1N2
10	A1B2C3D3E3F5G3H2I4J4K2L4M1N1	119	A13B6C5D7E3F4G4H1I2J3K2L2M3N2
11	A1B2C3D3E3F5G3H2I4J4K2L4M2N1	120	A5B8C5D6E3F4G2H4I2J2K1L4M1N2
12	A1B2C3D3E3F5G3H2I4J1K2L4M2N1	121	A1B4C4D2E4F6G3H2I4J4K3L4M1N2
13	A7B5C5D4E4F5G1H2I2J4K3L2M3N1	122	A10B4C7D3E4F7G5H4I1J4K2L4M1N2
14	A3B4C6D3E3F9G5H4I4J1K3L2M1N3	123	A10B4C4D5E5F2G5H2I4J3K3L2M2N2
15	A3B4C6D3E3F9G1H4I2J1K3L2M1N3	124	A3B4C5D5E7F2G5H2I4J3K2L2M2N4
16	A12B4C3D3E4F5G5H4I1J1K1L4M1N3	125	A8B9C4D3E7F6G3H2I4J2K3L4M3N1
17	A4B4C8D3E3F9G5H4I1J1K1L4M1N4	126	A12B5C4D3E4F4G5H4I1J2K2L2M1N3
18	A5B7C7D3E4F9G5H3I2J1K3L4M1N1	127	A13B5C2D3E4F4G5H4I1J2K2L2M1N3
19	A12B4C4D3E4F4G4H4I1J1K1L2M1N3	128	A12B4C6D3E4F7G5H4I1J2K2L4M1N4
20	A2B4C8D3E1F9G4H4I4J1K3L2M1N3	129	A1B9C2D3E7F6G4H2I4J4K3L4M2N2
21	A2B4C6D3E1F9G4H4I4J1K3L2M1N3	130	A1B9C2D3E4F6G4H2I4J3K3L2M2N3
22	A7B5C5D4E4F5G1H3I2J4K3L2M3N1	131	A4B9C1D5E7F6G3H2I1J4K3L4M2N2
23	A2B3C2D3E2F4G3H3I2J1K3L2M1N2	132	A11B4C4D5E4F2G6H2I4J3K3L2M2N2
24	A2B2C6D5E4F5G1H3I4J3K1L5M1N3	133	A4B4C6D5E7F6G6H2I4J4K2L4M2N2
25	A2B2C7D6E7F4G5H3I4J3K1L2M3N4	134	A3B5C6D4E1F7G5H4I4J3K3L2M2N3
26	A8B2C7D5E7F5G5H1I4J2K1L2M1N2	135	A4B4C5D5E4F5G5H2I4J2K2L4M0N2
27	A8B9C2D6E3F4G2H2I2J3K3L3M3N1	136	A3B4C3D3E3F2G4H2I4J3K3L4M2N3
28	A3B7C5D2E7F7G3H2I3J3K3L3M3N2	137	A8B4C4D0E7F4G2H2I4J3K2L2M1N4
29	A4B2C6D2E3F8G4H4I1J1K3L2M1N3	138	A2B0C4D1E6F6G3H2I4J3K3L2M2N3
30	A7B2C6D6E6F5G2H2I3J1K3L6M1N4	139	A3B4C6D5E7F2G5H2I4J2K2L2M3N4
31	A1B7C2D2E5F6G4H2I3J4K3L2M3N2	140	A9B3C4D6E4F2G2H2I3J4K3L4M3N2
32	A13B6C8D5E3F3G2H4I3J3K4L6M1N4	142	A4B9C4D3E4F4G4H2I4J4K2L4M1N2
33	A7B2C6D6E6F5G2H2I4J1K3L2M1N3	143	A13B4C4D3E4F4G4H4I1J1K1L2M1N3
34	A12B3C4D3E7F3G2H4I3J3K1L2M1N2	144	A4B9C2D2E7F6G3H2I4J4K3L4M3N2
35	A2B2C7D3E1F8G5H4I4J2K3L3M1N4	145	A13B4C6D3E3F7G4H4I1J1K2L2M1N3
36	A8B2C5D5E3F4G2H2I3J2K3L2M3N2	146	A2B9C2D3E4F6G3H4I1J1K1L2M3N2
37	A7B2C4D4E7F6G2H4I3J1K3L2M3N4	147	A11B2C3D3E2F2G6H4I1J4K1L2M3N3
38	A2B6C4D3E1F3G4H2I3J3K4L5M3N2	148	A4B4C8D3E3F9G5H4I1J1K1L4M1N3
39	A3B7C6D2E4F4G6H1I3J3K4L5M1N1	149	A4B4C6D5E6F2G4H2I4J2K2L2M3N4
40	A11B2C3D3E2F2G5H4I1J4K1L2M3N3	150	A4B8C3D5E3F2G5H2I4J1K1L2M3N3
41	A11B4C4D3E4F5G6H4I1J1K2L2M1N4	151	A5B3C2D9E1F4G3H2I3J3K4L5M1N1
42	A6B2C5D6E7F5G1H3I4J2K3L5M1N4	152	A13B5C5D3E3F4G5H2I1J1K1L4M1N3
43	A10B2C7D2E4F1G2H3I3J4K3L3M1N4	153	A4B4C4D5E2F4G1H2I4J0K2L4M1N1
44	A1B3C4D4E7F6G4H3I2J4K3L3M3N4	154	A5B3C3D9E2F3G2H2I4J3K3L4M1N3
45	A7B7C2D5E5F6G3H1I2J4K3L2M3N1	155	A3B3C9D8E2F2G3H1I4J3K4L4M1N1
46	A6B8C1D7E4F2G7H1I2J4K3L1M3N4	156	A10B3C4D8E2F3G2H2I4J3K2L4M1N3
47	A9B7C1D8E4F3G1H1I2J4K3L3M3N1	157	A13B5C5D4E2F4G5H2I1J1K1L3M1N3
48	A4B2C3D5E5F5G4H3I4J4K3L2M1N4	158	A3B2C2D2E6F3G7H3I2J3K3L4M3N4
49	A6B6C2D9E3F6G1H2I2J4K3L3M3N1	159	A5B7C4D3E4F9G5H4I2J1K3L4M1N1
50	A10B5C6D6E4F2G5H4I1J4K3L2M1N1	160	A9B8C6D3E3F3G2H1I2J3K3L4M3N2
51	A8B7C3D4E7F5G3H3I4J2K3L3M3N1	161	A2B8C2D8E1F3G3H1I2J3K3L4M3N2
52	A10B6C1D4E4F7G2H1I2J2K3L3M3N4	162	A2B4C3D3E6F3G3H3I3J1K3L4M3N2
53	A8B2C3D5E4F2G2H2I3J3K3L2M3N2	163	A4B3C1D2E1F3G4H1I2J3K2L4M1N4
54	A8B2C2D6E4F2G1H2I3J3K3L4M3N2	164	A4B3C2D3E1F3G4H1I2J3K2L4M1N3
55	A10B2C7D4E7F2G4H2I1J1K3L4M3N2	165	A6B2C5D6E6F2G1H3I3J1K3L2M1N1
56	A4B2C2D4E7F1G4H2I4J1K3L4M2N2	166	A8B2C5D5E3F4G2H2I3J2K3L4M3N2
57	A5B4C6D4E2F7G4H2I3J2K3L4M2N2	167	A6B7C2D5E3F3G3H1I3J3K3L6M1N2
58	A8B2C2D5E7F2G2H1I4J1K4L4M3N2	168	A9B8C4D6E2F3G7H2I2J4K2L2M3N3
59	A4B2C8D4E4F2G3H2I2J4K3L5M2N2	169	A12B10C5D5E2F3G7H2I2J4K2L4M3N3
60	A2B3C3D4E4F3G3H2I2J2K3L4M3N2	170	A8B9C2D6E2F3G7H2I2J4K2L4M5N3
61	A3B1C5D3E4F3G5H2I4J1K2L3M3N3	171	A6B10C1D5E2F3G2H2I2J3K2L2M3N2
62	A4B2C7D3E0F0G6H0I2J2K1L0M2N3	172	A7B8C6D2E6F3G3H4I1J3K2L3M2N3
63	A4B2C7D3E1F4G6H4I2J2K2L3M2N2	173	A5B3C7D5E7F4G4H4I3J3K2L4M1N4
64	A13B3C4D3E1F4G6H4I2J2K1L2M2N2	174	A10B7C1D5E2F3G4H2I3J4K2L2M3N3
65	A12B3C4D3E1F4G6H4I2J2K1L4M2N2	175	A5B9C6D6E2F7G4H2I3J2K2L4M3N3
66	A12B3C4D3E2F4G5H4I1J2K1L2M2N3	176	A9B7C3D6E4F2G2H2I3J4K2L1M3N4
67	A8B8C6D3E3F3G2H1I2J3K3L4M3N2	177	A8B9C1D2E7F3G3H2I4J2K2L4M3N3
68	A3B1C7D6E7F3G5H3I2J1K2L4M3N3	178	A10B9C3D5E3F3G2H2I3J3K3L4M3N3
69	A3B1C7D3E2F3G5H3I2J1K2L4M3N3	179	A13B3C6D6E2F2G5H2I3J3K3L4M3N3
70	A5B4C2D8E2F3G4H2I3J3K1L4M3N3	180	A5B9C3D4E2F8G2H2I3J3K4L4M3N2
71	A8B7C4D6E2F3G2H2I3J3K3L4M3N3	181	A2B1C6D6E5F2G1H3I4J2K4L6M1N1
72	A6B7C3D8E3F5G7H2I3J4K3L4M5N4	182	A8B1C6D6E5F2G4H3I4J2K4L6M1N1
73	A8B6C3D8E3F5G7H2I3J4K3L4M5N4	183	A5B7C4D7E5F3G4H1I2J2K4L4M3N3
74	A6B7C3D8E4F3G7H2I3J4K3L4M3N4	184	A5B8C3D9E6F4G4H2I2J3K2L4M3N2
75	A11B6C7D7E3F3G4H4I3J3K1L3M1N2	185	A13B6C4D9E2F2G5H2I2J3K2L2M3N3
77	A6B8C2D6E3F3G2H2I3J3K4L4M3N4	186	A9B7C4D7E1F4G2H2I2J3K2L2M3N4
78	A1B8C2D7E4F3G4H2I4J3K3L4M3N2	187	A9B7C4D7E1F4G2H2I2J4K1L2M3N4
79	A2B7C3D8E6F4G2H2I2J3K3L6M3N2	188	A2B7C1D8E1F2G4H2I2J4K1L2M3N2
80	A7B7C7D8E7F3G7H2I3J3K3L3M3N4	189	A6B9C2D4E2F2G7H2I2J3K1L4M5N4
81	A1B2C4D3E6F4G5H2I3J1K3L3M1N4	190	A5B7C2D8E2F2G7H2I2J3K1L4M5N2
82	A11B2C4D2E3F3G6H3I4J2K4L3M1N3	191	A7B7C4D8E5F6G5H2I2J3K1L4M3N3
83	A2B7C7D8E7F5G3H2I2J1K3L5M3N3	192	A6B1C4D8E6F4G6H2I2J1K1L2M3N4
84	A5B7C3D8E3F4G3H2I2J2K3L3M1N5	193	A5B7C6D8E6F2G7H2I2J3K1L2M3N3
85	A5B9C5D8E2F5G3H4I2J3K3L2M3N4	194	A6B7C2D9E2F5G7H2I1J4K3L4M3N3
86	A6B4C3D5E3F4G3H2I3J3K3L3M3N4	195	A6B7C2D9E2F5G7H2I2J4K2L2M3N4
87	A3B9C3D3E4F4G4H3I3J3K3L3M1N2	196	A1B7C6D8E5F5G3H3I2J1K2L4M3N2
88	A9B7C1D9E3F4G3H2I1J3K3L5M3N3	197	A4B7C6D8E5F2G7H2I2J3K2L2M3N3
89	A2B2C4D3E6F3G4H2I2J1K3L4M3N3	198	A5B7C2D9E2F2G3H2I2J3K3L2M3N2
90	A10B7C4D3E3F4G5H2I1J3K3L3M3N2	199	A5B7C2D9E1F2G3H2I2J3K2L2M3N2
91	A4B2C2D3E5F8G4H3I3J2K3L4M3N4	200	A5B7C2D9E1F2G2H2I2J3K2L2M3N2
92	A9B8C5D9E3F4G3H2I2J3K3L3M4N2	201	A5B9C2D8E2F1G2H2I2J3K2L1M3N3
93	A9B7C5D8E3F4G3H2I2J3K3L4M3N2	202	A5B7C2D9E2F5G7H3I2J4K2L2M3N3
94	A10B7C2D8E3F4G1H2I2J3K3L6M3N2	203	A5B7C2D9E2F2G2H2I2J3K3L2M3N2
95	A8B8C5D8E3F4G3H2I3J3K3L2M3N2	204	A5B7C2D9E2F2G2H2I1J3K2L2M3N2
96	A8B8C2D6E3F4G3H2I1J3K3L2M3N2	205	A13B2C4D3E4F5G6H3I1J1K1L2M1N3
97	A6B6C3D6E3F4G3H2I3J3K3L6M1N1	206	A2B7C2D8E6F4G2H2I1J3K3L6M3N2
98	A4B7C7D6E3F4G3H3I4J3K4L2M3N4	207	A2B7C2D8E6F4G2H2I1J3K2L6M3N2
99	A12B8C3D6E3F1G5H2I1J3K3L5M3N4	208	A1B2C6D3E1F8G5H4I3J2K2L3M1N3
100	A3B2C5D3E5F6G5H3I4J1K3L3M1N4	209	A1B1C6D6E6F2G6H3I3J2K1L3M3N4
101	A9B6C4D6E3F4G4H2I3J3K4L4M3N4	210	A2B1C6D6E6F2G6H3I3J2K1L3M3N4
102	A8B9C2D6E3F5G7H2I3J3K3L2M3N3	211	A6B2C5D3E4F4G7H2I3J3K2L3M3N2
103	A9B5C3D4E1F5G4H2I3J0K0L2M3N4	214	A3B2C2D7E4F4G7H2I0J3K2L3M3N2
104	A4B2C2D6E5F0G4H3I3J1K3L4M3N4	215	A1B7C7D5E7F3G4H3I4J2K2L4M3N2
105	A8B6C6D5E3F4G5H3I1J2K3L3M1N2	216	A5B6C4D9E5F4G2H2I4J4K2L2M3N3
106	A9B8C3D9E3F4G4H3I3J3K3L3M3N2	217	A3B1C8D6E6F5G5H3I0J0K4L4M3N3
107	A10B9C3D6E3F4G4H3I3J2K3L4M3N4	218	A1B1C2D4E4F3G3H1I4J3K2L4M1N2
108	A9B9C8D6E1F5G4H2I3J2K3L2M3N4	219	A1B1C8D6E5F5G5H3I4J2K4L4M3N3
109	A10B8C8D8E4F0G3H0I3J4K3L0M3N4	220	A1B1C2D4E4F3G3H1I4J3K2L4M1N7

Table 3

Fig. 5

Table S1

Tobacco germplasm resources"

编号No.	种质名称 Germplasm name	类型 Type	种质提供单位 Origin	来源 Source
1	Segedinska Ruca	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	南斯拉夫 Yugoslavia
2	Scafati	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	意大利 Italy
3	土耳其雪茄 Turkish cigar	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	土耳其 Turkey
4	Manila	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	菲律宾 the Philippines
5	Connecticut Shade	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	美国 USA
6	Bad Geudertheimer Landsorte	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	德国 Germany
7	H423	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	美国 USA
8	2042	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)
9	古巴2号 Cuba 2	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	古巴 Cuba
10	古巴2号-1 Cuba 2-1	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	古巴 Cuba
11	古巴2号-2 Cuba 2-2	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	古巴 Cuba
12	古巴2号-3 Cuba 2-3	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	古巴 Cuba
13	哈瓦娜 Hawana	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	古巴 Cuba
14	Havana IIc	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	波兰 Poland
15	Havana IIc	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	德国 Germany
16	Havana10	雪茄烟 Cigar tobacco	国家烟草种质资源中期库National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	波兰 Poland
17	Havana 211	雪茄烟 Cigar tobacco	国家烟草种质资源中期库National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	美国 USA
18	H211	雪茄烟 Cigar tobacco	国家烟草种质资源中期库National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	古巴 Cuba
19	Havana 38	雪茄烟 Cigar tobacco	国家烟草种质资源中期库National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	德国 Germany
20	Havana 510	雪茄烟 Cigar tobacco	国家烟草种质资源中期库National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	波兰 Poland
21	Havana Connecticut	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	美国 USA
22	夏湾那 Xiawanna	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	古巴 Cuba
23	Criollo Salteno 11	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	巴西 Brazil
24	多米尼加短芯 Dominican Short Core Leaf	雪茄烟 Cigar tobacco	德阳市烟草公司 Deyang Tobacco Company	多米尼加 Dominican
25	多米尼加长芯Dominican Long Core Leaf	雪茄烟 Cigar tobacco	德阳市烟草公司 Deyang Tobacco Company	多米尼加 Dominican
26	尼加拉瓜短芯 Nigaragua Short Core Leaf	雪茄烟 Cigar tobacco	德阳市烟草公司 Deyang Tobacco Company	尼加拉瓜 Nicaragua
27	H382	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	印度尼西亚 Indonesia
28	J 20	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)
29	Little Dutch	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	美国 USA
30	Oosikappal	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)
31	Zimmer Spanish	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	西班牙 Spain
32	Zrenjanin	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	南斯拉夫 Yugoslavia
33	铁杆青 Tieganqing	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国贵州 Guizhou, China
34	Sumatra Mitlingskaja	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	印度尼西亚 Indonesia
35	Friedrichstaier	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	德国 Germany
36	Florida 301	雪茄烟 Cigar tobacco	国家烟草种质资源中期库National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	美国 USA
37	E 18	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	德国 Germany
38	Dirojuio Fagues No.361	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	波兰 Poland
39	Copus Pobrecene No.1241	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	波兰 Poland
40	Connecticut Broad Leaf	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	美国 USA
41	Comstock Spanish	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	西班牙 Spain
42	Beinhart 1000-1	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	美国 USA
43	Begej	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	南斯拉夫 Yugoslavia
44	422 (Kedoe Hybrid 43)	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)
45	112-117	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	美国 USA
46	什邡70-30号 Shifang70-30	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国四川 Sichuan, China
47	小牛舌 Xiaoniushe	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国江西 Jiangxi, China
48	Conn Shade	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	美国 USA
49	Bel-W3	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	美国 USA
50	诺凡斯瑞加 Nuofansiruijia	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)
51	Florida 513	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	美国 USA
52	世纪1号 Shiji 1	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国浙江 Zhejiang, China
53	2052	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)
54	印尼雪茄包皮 Indonesia cigar wrapper	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	印度尼西亚 Indonesia
55	印尼02号 Indonesia 02	雪茄烟 Cigar tobacco	国家烟草种质资源中期库National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	印度尼西亚 Indonesia
56	无名雪茄 Wuming cigar	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)
57	Trapezund 610	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	波兰 Poland
58	Trapesond 288	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	俄罗斯 Russia
59	Sumatra Deli	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	印度尼西亚 Indonesia
60	Pennbel 69	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	美国 USA
61	Pennsylvania Seed Leaf	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	美国 USA
62	Philippin	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	菲律宾 Philippines
63	Mont Calme Brun	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)
64	Lanka 23	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)
65	Hanica	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	德国 Germany
66	Geudetthelmex	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	德国 Germany
67	2040	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)
68	Burley 21	白肋烟 Burley	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	美国 USA
69	Burley 37	白肋烟 Burley	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	美国 USA
70	沂水大弯筋 Yishuidawanjin	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国山东 Shandong, China
71	塘蓬 Tangpeng	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国广东 Guangdong, China
72	什新-2 Shixin-2	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国四川 Sichuan, China
73	什新-1 Shixin-1	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国四川 Sichuan, China
74	宣汉晒烟76-2 Xuanhanshaiyan 76-2	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国四川 Sichuan, China
75	山东大叶 Shandongdaye	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国山东 Shandong, China
76	牡丹江05-1 Mudanjiang 05-1	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国黑龙江 Heilongjiang, China
77	利川乌烟 Lichuanwuyan	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国湖北 Hubei, China
78	罗三湾青毛烟 Luosanwanqingmaoyan	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国湖北 Hubei, China
79	桐乡晒烟 Tongxiangshaiyan	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国浙江 Zhejiang, China
80	NY-3	晒烟 Sun-cured tobacco	德阳市烟草公司 Deyang Tobacco Company	印度尼西亚 Indonesia
81	公会晒烟 Gonghuishaiyan	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国广西 Guangxi, China
82	万毛1号 Wanmao 1	晒烟 Sun-cured tobacco	德阳市烟草公司 Deyang Tobacco Company	中国重庆 Chongqing, China
83	万毛2号 Wanmao 2	晒烟 Sun-cured tobacco	德阳市烟草公司 Deyang Tobacco Company	中国重庆 Chongqing, China
84	龙井香叶子 Longjingxiangyezi	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国吉林 Jilin, China
85	凤凰香烟 Fenghuangxiangyan	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国湖南 Hunan, China
86	黄苗2220 Huangmiao 2220	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国河南 Henan, China
87	新宾小团叶 Xinbinxiaotuanye	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国辽宁 Liaoning, China
88	督叶尖杆种 Duyejianganzhong	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国浙江 Zhejiang, China
89	红花铁杆子 Honghuatieganzi	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国四川 Sichuan, China
90	尚志一朵花 Shangzhiyiduohua	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国黑龙江 Heilongjiang, China
91	什邡枇杷柳 Shifangpipaliu	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国四川 Sichuan, China
92	伟俄小柳叶 Weiexiaoliuye	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国贵州 Guizhou, China
93	铁赤烟 Tiechiyan	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国江西 Jiangxi, China
94	丹阳烟 Danyangyan	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国江苏 Jiangsu, China
95	人和烟 Renheyan	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国广东 Guangdong, China
96	云罗03 Yunluo 03	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国广东 Guangdong, China
97	稀格巴小黑烟 Xigebaxiaoheiyan	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国贵州 Guizhou, China
98	辽多叶 Liaoduoye	晒烟 Sun-cured tobacco	国家烟草种质资源中期库National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国辽宁 Liaoning, China
99	江油烟 Jiangyouyan	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国四川 Sichuan, China
100	吉县大烟叶子 Jixiandayanyezi	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国山西 Shanxi, China
101	巫山小兰烟 Wushanxiaolanyan	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国四川 Sichuan, China
102	湄潭晒烟 Meitanshaiyan	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国贵州 Guizhou, China
103	鹤峰黑烟 Hefengheiyan	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国湖北 Hubei, China
104	铁板青 Tiebanqing	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国湖北 Hubei, China
105	泌阳晒烟(4) Biyangshaiyan (4)	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国河南 Henan, China
106	六峰毛把烟 Liufengmaobayan	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国湖北 Hubei, China
107	阳和大乌烟 Yanghedawuyan	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国湖北 Hubei, China
108	建平瓢把烟 Jianpingpiaobayan	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国四川 Sichuan, China
109	巫山大南烟-1 Wushandananyan-1	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国四川 Sichuan, China
110	早阳人头烟 Zaoyangrentouyan	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国四川 Sichuan, China
111	丛树大青烟 Congshudaqingyan	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国重庆 Chongqing, China
112	大叶子青烟 Dayeziqingyan	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国重庆 Chongqing, China
113	巫溪火烟-2 Wuxihuoyan-2	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国重庆 Chongqing, China
114	利川毛烟 Lichuanmaoyan	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国湖北 Hubei, China
115	竹山大柳子 Zhushandaliuzi	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国湖北 Hubei, China
116	川柳子烟 Chuanliuziyan	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国四川 Sichuan, China
117	Wisconsin38	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	美国 USA
118	Q-5-0082	药烟 Herbal medicine tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国山西 Shanxi, China
119	81-8-6	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国广东 Guangdong, China
120	A-0014	药烟 Herbal medicine tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国山西 Shanxi, China
121	Havana-3	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	古巴 Cuba
122	Havana-1	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	古巴 Cuba
123	Havana 1	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	古巴 Cuba
124	S-2	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	古巴 Cuba
125	Florida 513	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	美国 USA
126	Geudetthelmex	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	德国 Germany
127	Havana 10	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	波兰 Poland
128	土耳其雪茄烟 Turkish cigar	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	土耳其 Turkey
129	德雪1号 Dexue 1	雪茄烟 Cigar tobacco	德阳市烟草公司 Deyang Tobacco Company	古巴 Cuba
130	德雪2号 Dexue 2	雪茄烟 Cigar tobacco	德阳市烟草公司 Deyang Tobacco Company	古巴 Cuba
131	德雪3号 Dexue 3	雪茄烟 Cigar tobacco	德阳市烟草公司 Deyang Tobacco Company	古巴 Cuba
132	Habana92	雪茄烟 Cigar tobacco	德阳市烟草公司 Deyang Tobacco Company	古巴 Cuba
133	C-15	雪茄烟 Cigar tobacco	德阳市烟草公司 Deyang Tobacco Company	古巴 Cuba
134	青州一号 Qingzhou 1	雪茄烟 Cigar tobacco	德阳市烟草公司 Deyang Tobacco Company	中国 China
135	Cubra-Brazil	雪茄烟 Cigar tobacco	德阳市烟草公司 Deyang Tobacco Company	巴西 Brazil
136	美国康州阔叶 America Connecticut Broadleaf	雪茄烟 Cigar tobacco	德阳市烟草公司 Deyang Tobacco Company	美国 USA
137	OLOR	雪茄烟 Cigar tobacco	德阳市烟草公司 Deyang Tobacco Company	多米尼加 Dominican
138	来凤H382 Laifeng H382	雪茄烟 Cigar tobacco	德阳市烟草公司 Deyang Tobacco Company	中国来凤 Laifeng, China
139	海南-1 Hainan-1	雪茄烟 Cigar tobacco	德阳市烟草公司 Deyang Tobacco Company	中国海南 Hainan, China
140	海南-2 Hainan-2	雪茄烟 Cigar tobacco	德阳市烟草公司 Deyang Tobacco Company	中国海南 Hainan, China
141	CA0701	雪茄烟 Cigar tobacco	德阳市烟草公司 Deyang Tobacco Company	中国浙江 Zhejiang, China
142	CA0702	雪茄烟 Cigar tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	美国 USA
143	CA0703	雪茄烟 Cigar tobacco	德阳市烟草公司 Deyang Tobacco Company	美国 USA
144	CA0704	雪茄烟 Cigar tobacco	德阳市烟草公司 Deyang Tobacco Company	美国 USA
145	CA0705	雪茄烟 Cigar tobacco	德阳市烟草公司 Deyang Tobacco Company	波兰 Poland
146	CA0706	雪茄烟 Cigar tobacco	德阳市烟草公司 Deyang Tobacco Company	德国 Germany
147	CA0708	雪茄烟 Cigar tobacco	德阳市烟草公司 Deyang Tobacco Company	美国 USA
148	CA0709	雪茄烟 Cigar tobacco	德阳市烟草公司 Deyang Tobacco Company	波兰 Poland
149	尼加拉瓜长芯 Nicaragua Long Core Leaf	雪茄烟 Cigar tobacco	德阳市烟草公司 Deyang Tobacco Company	尼加拉瓜 Nicaragua
150	哈珀纳斯 Haponasi	雪茄烟 Cigar tobacco	德阳市烟草公司 Deyang Tobacco Company	古巴 Cuba
151	MFPP	雪茄烟 Cigar tobacco	德阳市烟草公司 Deyang Tobacco Company	巴西 Brazil
152	MFZS	雪茄烟 Cigar tobacco	德阳市烟草公司 Deyang Tobacco Company	巴西 Brazil
153	CP2011	雪茄烟 Cigar tobacco	德阳市烟草公司 Deyang Tobacco Company	巴西 Brazil
154	MSCA	雪茄烟 Cigar tobacco	德阳市烟草公司 Deyang Tobacco Company	多米尼加 Dominica
155	MNC	雪茄烟 Cigar tobacco	德阳市烟草公司 Deyang Tobacco Company	多米尼加 Dominica
156	DM长 DM Chang	雪茄烟 Cigar tobacco	德阳市烟草公司 Deyang Tobacco Company	多米尼加 Dominica
157	CS0701	雪茄烟 Cigar tobacco	德阳市烟草公司 Deyang Tobacco Company	菲律宾 the Philippines
158	CS0704	雪茄烟 Cigar tobacco	德阳市烟草公司 Deyang Tobacco Company	中国广东 Guangdong, China
159	CS0705	雪茄烟 Cigar tobacco	德阳市烟草公司 Deyang Tobacco Company	中国广东 Guangdong, China
160	CS0707	雪茄烟 Cigar tobacco	德阳市烟草公司 Deyang Tobacco Company	中国四川 Sichuan, China
161	CS0708	雪茄烟 Cigar tobacco	德阳市烟草公司 Deyang Tobacco Company	中国山东 Shandong, China
162	CS0709	雪茄烟 Cigar tobacco	德阳市烟草公司 Deyang Tobacco Company	中国四川 Sichuan, China
163	CS0713	雪茄烟 Cigar tobacco	德阳市烟草公司 Deyang Tobacco Company	中国四川 Sichuan, China
164	3763	雪茄烟 Cigar tobacco	德阳市烟草公司 Deyang Tobacco Company	中国四川 Sichuan, China
165	beinhart1000-1	雪茄烟 Cigar tobacco	德阳市烟草公司Deyang Tobacco Company	美国 USA
166	florida30	雪茄烟 Cigar tobacco	德阳市烟草公司 Deyang Tobacco Company	美国 USA
167	稀格巴小黑烟 Xigebaxiaoheiyan	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国贵州 Guizhou, China
168	高产烟-3 Gaochanyan-3	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国广东 Guangdong, China
169	包盖烟-1 Baogaiyan-1	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国广东 Guangdong, China
170	四川青毛 Sichuanqingmao	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国四川 Sichuan, China
171	青毛籽 Qingmaozi	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国四川 Sichuan, China
172	四川黄毛 Sichuanhuangmao	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国四川 Sichuan, China
173	奉节大毛烟 Fengjiedamaoyan	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国重庆 Chongqing, China
174	大火柳子烟 Dahuoliuziyan	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国湖北 Hubei, China
175	黄金烟 Huangjinyan	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国湖南 Hunan, China
176	青梗8 Qinggeng 8	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国广东 Guangdong, China
177	泡杆烟 Paoganyan	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国贵州 Guizhou, China
178	云南荼科所晒烟 Yunnantukesuoshanyan	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国云南 Yunnan, China
179	牡晒81-21-2 Mushai 81-21-2	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国黑龙江 Heilongjiang, China
180	州852 Zhou 852	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国湖南 Hunan, China
181	贝拉烟 Beilayan	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	几内亚 Guinea
182	牡晒89-23-1 Mushai 89-23-1	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国黑龙江 Heilongjiang, China
183	晒9118 Shai 9118	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国湖南 Hunan, China
184	81-8-6	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国广东 Guangdong, China
185	清远牛利 Qingyuanniuli	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国广东 Guangdong, China
186	葵烟 Kuiyan	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国广东 Guangdong, China
187	湘潭细叶枇杷 Xiangtanxiyepipa	晒烟 Sun-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingd ao)	中国湖南 Hunan, China
188	什烟一号 Shiyan No.1	晒烟 Sun-cured tobacco	德阳市烟草公司 Deyang Tobacco Company	中国四川 Sichuan, China
189	葵柳 Kuiliu	晒烟 Sun-cured tobacco	德阳市烟草公司 Deyang Tobacco Company	中国四川 Sichuan, China
190	笆毛柳 Bamaoliu	晒烟 Sun-cured tobacco	德阳市烟草公司 Deyang Tobacco Company	中国四川 Sichuan, China
191	绵竹晒烟 Mianzhushaiyan	晒烟 Sun-cured tobacco	德阳市烟草公司 Deyang Tobacco Company	中国四川 Sichuan, China
192	GW3	晒烟Sun-cured tobacco	德阳市烟草公司 Deyang Tobacco Company	中国四川 Sichuan, China
193	MJ1	晒烟Sun-cured tobacco	德阳市烟草公司 Deyang Tobacco Company	中国四川 Sichuan, China
194	MJ2	晒烟 Sun-cured tobacco	德阳市烟草公司 Deyang Tobacco Company	中国四川 Sichuan, China
195	巴山一号 Bashan 1	晒烟 Sun-cured tobacco	德阳市烟草公司 Deyang Tobacco Company	中国四川 Sichuan, China
196	万毛3号 Wanmao 3	晒烟 Sun-cured tobacco	德阳市烟草公司 Deyang Tobacco Company	中国重庆 Chongqing, China
197	崇州晒烟1 Chongzhoushaiyan 1	晒烟 Sun-cured tobacco	德阳市烟草公司 Deyang Tobacco Company	中国四川 Sichuan, China
198	崇州晒烟2 Chongzhoushaiyan 2	晒烟 Sun-cured tobacco	德阳市烟草公司 Deyang Tobacco Company	中国四川 Sichuan, China
199	州烟 Zhouyan	晒烟 Sun-cured tobacco	德阳市烟草公司 Deyang Tobacco Company	中国四川 Sichuan, China
200	铁杆子 Tieganzi	晒烟 Sun-cured tobacco	德阳市烟草公司 Deyang Tobacco Company	中国四川 Sichuan, China
201	黄毛烟 Huangmaoyan	晒烟 Sun-cured tobacco	德阳市烟草公司 Deyang Tobacco Company	中国四川 Sichuan, China
202	白毛烟 Baimaoyan	晒烟 Sun-cured tobacco	德阳市烟草公司 Deyang Tobacco Company	中国四川 Sichuan, China
203	杂交晒烟 Zajiaoshaiyan	晒烟 Sun-cured tobacco	德阳市烟草公司 Deyang Tobacco Company	中国四川 Sichuan, China
204	白花 Bahua	晒烟 Sun-cured tobacco	德阳市烟草公司 Deyang Tobacco Company	中国河南 Henan, China
205	江西晒烟 Jiangxishaiyan	晒烟 Sun-cured tobacco	德阳市烟草公司 Deyang Tobacco Company	中国江西 Jiangxi, China
206	松阳晒烟 Songyangshaiyan	晒烟 Sun-cured tobacco	德阳市烟草公司 Deyang Tobacco Company	中国浙江 Zhejiang, China
207	连选晒烟 Lianxuanshaiyan	晒烟 Sun-cured tobacco	德阳市烟草公司 Deyang Tobacco Company	中国广东 Guangdong, China
208	城口晒烟 Chengkoushaiyan	晒烟 Sun-cured tobacco	德阳市烟草公司 Deyang Tobacco Company	中国重庆 Chongqing, China
209	4	晒烟 Sun-cured tobacco	德阳市烟草公司 Deyang Tobacco Company	中国四川 Sichuan, China
210	5	晒烟 Sun-cured tobacco	德阳市烟草公司 Deyang Tobacco Company	中国四川 Sichuan, China
211	良种 Liangzhong	晒烟 Sun-cured tobacco	德阳市烟草公司 Deyang Tobacco Company	中国湖南 Hunan, China
212	辅善 Fushan	晒烟 Sun-cured tobacco	德阳市烟草公司 Deyang Tobacco Company	中国四川 Sichuan, China
213	满耳朵 Manerduo	晒烟 Sun-cured tobacco	德阳市烟草公司 Deyang Tobacco Company	中国四川 Sichuan, China
214	青梗 Qinggeng	晒烟 Sun-cured tobacco	德阳市烟草公司 Deyang Tobacco Company	中国广东 Guangdong, China
215	南雄晒黄烟 Nanxiongshaihuangyan	晒烟 Sun-cured tobacco	德阳市烟草公司 Deyang Tobacco Company	中国广东 Guangdong, China
216	泉烟 Quanyan	晒烟 Sun-cured tobacco	德阳市烟草公司 Deyang Tobacco Company	中国四川 Sichuan, China
217	Maryland609	马里兰烟 Maryland tobacco	德阳市烟草公司 Deyang Tobacco Company	美国 USA
218	TN86	白肋烟 Burley	德阳市烟草公司 Deyang Tobacco Company	美国 USA
219	81-26	晒烟 Sun-cured tobacco	德阳市烟草公司 Deyang Tobacco Company	中国黑龙江 Heilongjiang, China
220	武鸣牛利 Wumingniuli	晾烟 Air-cured tobacco	国家烟草种质资源中期库 National Infrastructure for Crop Germplasm Resources (Tobacco, Qingdao)	中国广西 Guangxi, China

Table S1

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