Table 1

Analysis of physicochemical properties, subcellular localizations, and signal peptides of SsTLP family proteins"

Fig. 1

Chromosome location of SsTLP family genes"

Fig. 2

Gene structure of SsTLP family The green box representes the coding sequence (CDS); the yellow box representes the untranslated region (UTR); the horizontal line in the middle of the box representes the intron; 0, 1, and 2 representes the intron phase."

Fig. 3

Conservation motif analysis of SsTLP family The boxes of different colors represent the motif types and positions."

Fig. 4

Phylogenetic tree of SsTLP family The red dot representes the Saccharum spontaneum SsTLP protein; the green dot representes the Arabidopsis thaliana AtTLP protein; the black dot representes the Oryza sativa OsTLP protein."

Fig. 5

Nucleic acid sequence and the coding amino acid sequence of ScTLP1 gene The blue font is the cysteine site; the box sequence is the THN conservative domain; the yellow sequence is the signal peptide; * representes stop codon."

Fig. 6

Multiple alignment of amino acid sequences of sugarcane ScTLP1 protein and TLP proteins of other homologous species"

Fig. 7

Tissue specific expression of ScTLP1 gene and its expression under different environmental stresses A: tissue-specific expression of ScTLP1 gene in sugarcane ROC22. R: root; SP: stem pith; L: leaf; SE: stem epidermis; B: bud. B: expression of ScTLP1 gene after inoculation with Sporisorium scitamineum. C-H: expression of ScTLP1 gene under exogenous hormones and abiotic stresses. ABA: abscisic acid; SA: salicylic acid; MeJA: methyl jasmonate; NaCl: sodium chloride; PEG: polyethylene glycol; Low temperature: 4°C low temperature treatment. The error bars represent the standard error of each group treatment (n = 3). Different lowercase letters above the bars mean significant differences at the 0.05 probability level."

Fig. 8

Transient expression effect of ScTLP1 gene in Nicotiana benthamiana leaves A: gene expression level of ScTLP1 in N. benthamiana after transient expression for 1 d. B: gene expression level of tobacco immune related genes in control group and experimental group after transient expression for 1 d. C: the DAB staining result of N. benthamiana leaves after transient expression for 2 d. D-E: the leaf phenotypes of N. benthamiana inoculated with Pseudomonas solanacearum and Fusarium solani var. coeruleum for seven days, respectively. The experimental data were normalized by the expression level of NtEF-1α gene. The error bars represent the standard errors of each group treatment (n = 3). Different lowercase letters above the bars mean significant differences at the 0.05 probability level."

Fig. 9

Subcellular localization of ScTLP1 protein in the epidermis of Nicotiana benthamiana The results include photographs taken from three perspectives, including visual field, green fluorescence, and merged field. The arrows a, b, and c represent the nucleus, cell membrane, and cytoplasm, respectively. 35S::GFP is the result of N. benthamiana injection with GV3101 bacterial solution containing subcellular localization empty vector; 35S::ScTLP1::GFP is the result of N. benthamiana injection with GV3101 bacterial solution containing and the recombinant subcellular localization vector with the target gene ScTLP1. Bar: 50 μm."

Fig. 10

Validation of transcriptional self-activation activity of ScTLP1 protein A: the growth of Y2H Gold yeast solution sprayed on SD-Trp solid medium with different concentrations of positive control, negative control and pGBKT7-ScTLP1. B: colonial staining result of SD-Trp solid culture medium with X-α-gal staining solution. C: colonial staining result after adding SD-Trp solid culture with X-α-gal staining solution. pGADT7-T+pGBKT7-p53: positive control; pGBKT7: negative control."

Table 1

Secondary structure prediction of SsTLP family proteins (%)"