不同耐旱性青稞叶片差异蛋白分析

doi:10.3724/SP.J.1006.2021.01062

Abstract

Abstract:

In order to reveal the differences in response to drought stress among different hulless barley varieties from the protein level and to analyze the protein molecular mechanism of drought tolerance, Handizi Barley (HDZ) resistant to drought stress and Dama Barley (DM) sensitive to drought stress were used as research materials in this study. Drought treatment were determined by potted-planting method with limited water supply, four physiological indexes of hulless barley leaves with different drought gradients, including chlorophyll, soluble protein, malondialdehyde content, and relative conductivity were investigated. iTRAQ technology was used to conduct differential protein analysis on the whole protein group of barley leaves under deep drought stress. The results showed that with the extension of the drought treatment, the chlorophyll and soluble protein content of two hulless barleys under drought stress gradually decreased, the electrical conductivity and malondialdehyde content gradually increased, and the decrease in chlorophyll and soluble protein content, the increase in electrical conductivity and the content of malondialdehyde in Dama were greater than that of Handizi; 4163 proteins (polypeptides) were quantified, among them, compared with normal culture in the Handizi comparison group, 68 up-regulated proteins and 63 down-regulated proteins were screened by iTRAQ; in the comparative group of Dama, 21 up-regulated proteins and 32 down-regulated proteins were screened. KEGG pathway showed that the top three enrichment pathways were metabolic, amino acid biosynthesis, and secondary metabolite biosynthesis. The first one mainly related to citric acid cycle, carbon cycle, and other metabolic pathways. The synthesis and degradation of amino acids were mainly involved arginine and alanine. The synthesis of secondary metabolites were about arachidonic acid and linolenic acid. This study screened the proteins related to the metabolic pathways and other related functions in response to drought stress on proteome level in hulless barley, providing a theoretical basis for revealing the molecular regulation mechanism in response to drought stress.

Key words: hulless barley, drought stress, leaf, differentially expressed protein, iTRAQ

LI Jie, FU Hui, YAO Xiao-Hua, WU Kun-Lun. Differentially expressed protein analysis of different drought tolerance hulless barley leaves[J].Acta Agronomica Sinica, 2021, 47(7): 1248-1258.

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处理 Treatment	品种 Variety	蛋白质含量 Protein content (mg g^-1)	相对电导率 Relative electrical conductivity (%)	丙二醛含量 Malondialdehyde content (μmoL g^-1)
对照 CK	旱地紫 HDZ	7.30±0.10 Aa	44.50±0.68 Cc	32.92±0.45 Cc
对照 CK	大麻 DM	10.00±0.56 Aa	50.10±0.34 Cc	28.70±0.10 Cc
干旱处理7 d 7 days after drought treatment	旱地紫 HDZ	6.90±0.55 Aa	45.89±0.47 Bb	35.55±0.29 Bb
干旱处理7 d 7 days after drought treatment	大麻 DM	7.87±0.12 Bb	54.33±0.60 Bb	32.21±0.38 Bb
干旱处理10 d 10 days after drought treatment	旱地紫 HDZ	6.75±0.26 Aa	48.97±0.71 Aa	38.92±0.42 Aa
干旱处理10 d 10 days after drought treatment	大麻 DM	7.65±0.61 Bb	60.22±0.39 Aa	40.33±0.48 Aa

比较组 Comparison group	肽段覆盖率 Protein coverage (%)							总计 Total
比较组 Comparison group	≤10	10<X≤20	20<X≤30	30<X≤40	40<X≤50	50<X≤60	60<X≤70	总计 Total
HDZD-HDZCK	650	807	409	192	64	18	8	2150
DMD-DMCK	622	752	390	170	55	16	6	2013

蛋白质功能分类 Functional classification of proteins	蛋白质数量 Number of proteins
胁迫应答类 Stress response	11
脂类代谢相关类 Lipid metabolism	13
糖类代谢相关类 Carbohydrate metabolism	10
蛋白质代谢相关类 Protein metabolism	48
氧化还原类 Oxidoreduction	35
能量供应类 Energy	16
光合相关类 Photosynthetic	15
未知蛋白 Unknown functional protein	36

通路ID Pathway ID	通路名称 Pathway name
ko00020	三羧酸循环 TCA cycle
ko00640	丙酸酯代谢 Propanoate metabolism
ko01200	碳代谢 Carbon metabolism
ko00270	半胱氨酸和蛋氨酸代谢 Cysteine and methionine metabolism
ko00620	丙酮酸代谢 Pyruvate metabolism
ko00630	乙醛酸和二羧酸的代谢 Glyoxylate and dicarboxylate metabolism
ko00710	光合作用中的碳固定 Carbon fixation in photosynthetic
ko00195	光合作用 Photosynthsis
ko03010	核糖体 Ribosome
ko03013	RNA转运 RNA transport
ko00190	氧化磷酸化 Oxidaxtive phosphorylation
ko04145	吞噬作用 Phagosome
ko04141	内质网的蛋白质加工 Protein processing in endoplasmic reticulum

Differentially expressed protein analysis of different drought tolerance hulless barley leaves

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