普通小麦颖壳蜡质缺失突变体glossy1的转录组分析

doi:10.3724/SP.J.1006.2022.11006

Abstract

Abstract:

In order to clarify the molecular mechanism of the significant change in glume wax content, transcriptome profiling was performed on glossy glume mutant glossy1 and its wild-type Jimai 22. The results showed that a total of 12,230 differentially expressed genes were screened in the glossy1 mutant, among which 5811 genes were up-regulated and 6419 were down-regulated. GO functional enrichment revealed that the differentially expressed genes were mainly enriched in the wax synthesis and transport pathway, specifically distributed in acyltransferase activity, lipid binding, hydrolase activity. The findings suggested that these pathways were closely related to the glume-wax-deficient trait in wheat. We also detected the relative expression levels of some genes involved in the wax metabolic pathway by RT-qPCR, and the results were consistent with the transcriptome data. This study not only provides data support for the further study of the molecular mechanism of wax metabolism and gene regulation network but also lays a theoretical foundation for the breeding of stress-resistant variety in wheat.

Key words: wheat, epicuticular wax, glossy1 mutant, transcriptome analysis, molecular mechanism

LI Ling-Hong, ZHANG Zhe, CHEN Yong-Ming, YOU Ming-Shan, NI Zhong-Fu, XING Jie-Wen. Transcriptome profiling of glossy1 mutant with glossy glume in common wheat (Triticum aestivum L.)[J].Acta Agronomica Sinica, 2022, 48(1): 48-62.

Figures/Tables 9

Fig. 1

Table 1

Table 2

Fig. 2

Fig. 3

Fig. 4

Fig. 5

Table 3

Differentially expressed genes related to epicuticular wax biosynthesis pathway in glumes of wheat"

基因编号 Gene ID	倍数变化 log₂ (Fold Change)	较正后P值 P_adj	显著性Significant	相关功能 Related functions	基因符号 Gene symbol
TraesCS5A02G242600	3.609865613	0.004916338	Up	Alpha/beta-hydrolases superfamily protein	DMH
TraesCS5B02G235400	1.489558363	7.57276E-14	Up	Alpha/beta-hydrolases superfamily protein	DMH
TraesCS5B02G239300	1.941903403	1.76388E-07	Up	Alpha/beta-hydrolases superfamily protein	DMH
TraesCS5D02G247700	1.416956174	4.56687E-23	Up	Alpha/beta-hydrolases superfamily protein	DMH
TraesCS5D02G249600	1.266778647	1.82079E-05	Up	Alpha/beta-hydrolases superfamily protein	DMH
TraesCS2A02G025700	1.613510275	7.96739E-37	Up	Chalcone synthase	CHS
TraesCS2A02G035300	6.307119168	4.38659E-26	Up	Chalcone synthase	CHS
TraesCS2A02G527700	1.584580826	7.22601E-67	Up	Chalcone synthase	CHS
TraesCS2B02G038700	1.511159327	2.49811E-44	Up	Chalcone synthase	CHS
TraesCS2B02G048400	6.771018546	4.02053E-13	Up	Chalcone synthase	CHS
TraesCS2B02G558400	1.565811929	6.38829E-60	Up	Chalcone synthase	CHS
TraesCS2D02G027700	7.885041654	2.73423E-09	Up	Chalcone synthase	CHS
TraesCS2D02G027900	1.089916323	2.10199E-17	Up	Chalcone synthase	CHS
TraesCS2D02G530600	1.359178980	5.17325E-33	Up	Chalcone synthase	CHS
TraesCS2A02G006400	1.163488023	4.09358E-15	Up	Cytochrome P450 family protein, expressed	DMC
TraesCS2B02G002000	1.292392885	2.02883E-05	Up	Cytochrome P450 family protein, expressed	DMC
TraesCS2D02G015600	5.598114975	6.08904E-16	Up	Cytochrome P450 family protein, expressed	DMC
TraesCS3B02G006600	2.974023949	3.86406E-80	Up	Cytochrome P450 family protein, expressed	DMC
TraesCS3D02G077400	3.854291668	3.1974E-209	Up	Cytochrome P450 family protein, expressed	DMC
TraesCS4A02G063900	1.997077843	1.68199E-10	Up	Cytochrome P450 family protein, expressed	DMC
TraesCS4A02G064000	1.370399465	4.5991E-19	Up	Cytochrome P450 family protein, expressed	DMC
TraesCS4B02G172200	2.400073652	9.20159E-06	Up	Cytochrome P450 family protein, expressed	DMC
TraesCS4B02G241100	1.849837854	2.89613E-18	Up	Cytochrome P450 family protein, expressed	DMC
TraesCS4B02G342000	1.906423178	9.65609E-98	Up	Cytochrome P450 family protein, expressed	DMC
TraesCS4D02G240800	2.345669375	1.52442E-13	Up	Cytochrome P450 family protein, expressed	DMC
TraesCS6A02G143700	2.852491021	1.56916E-05	Up	Cytochrome P450 family protein, expressed	DMC
TraesCS6A02G144600	2.291592568	3.17585E-37	Up	Cytochrome P450 family protein, expressed	DMC
TraesCS6A02G323900	5.510836360	1.0995E-08	Up	Cytochrome P450 family protein, expressed	DMC
TraesCS6D02G133500	5.032820286	7.9837E-09	Up	Cytochrome P450 family protein, expressed	DMC
TraesCS6D02G303700	2.939258272	6.1412E-12	Up	Cytochrome P450 family protein, expressed	DMC
TraesCS7A02G077500	1.399996805	0.004103167	Up	Cytochrome P450 family protein, expressed	DMC
基因编号 Gene ID	倍数变化 log₂ (Fold Change)	较正后P值 P_adj	显著性Significant	相关功能 Related functions	基因符号 Gene symbol
TraesCS7D02G028900	5.180466519	2.44704E-11	Up	Cytochrome P450 family protein, expressed	DMC
TraesCS7D02G073100	1.905576368	7.80242E-05	Up	Cytochrome P450 family protein, expressed	DMC
TraesCS1A02G075000	1.598873518	4.60281E-26	Up	Long-chain acyl-CoA synthetase	LACS
TraesCS1B02G093600	1.508738506	3.6507E-31	Up	Long-chain acyl-CoA synthetase	LACS
TraesCS1D02G077600	1.081329758	3.35804E-18	Up	Long-chain acyl-CoA synthetase	LACS
TraesCS4B02G110600	-1.547038060	5.29477E-09	Down	Long-chain acyl-CoA synthetase	LACS
TraesCS5A02G411500	-6.237682795	7.2037E-25	Down	Long-chain acyl-CoA synthetase	LACS
TraesCS5B02G415100	-8.272718606	9.87304E-27	Down	Long-chain acyl-CoA synthetase	LACS
TraesCS5D02G420300	-4.365756350	6.03687E-49	Down	Long-chain acyl-CoA synthetase	LACS
TraesCS1A02G037600	4.524213496	0.000135779	Up	3-ketoacyl-CoA synthase	KCS
TraesCS1A02G439000	2.761509645	8.73425E-44	Up	3-ketoacyl-CoA synthase	KCS
TraesCS1B02G048000	5.015626497	1.73996E-16	Up	3-ketoacyl-CoA synthase	KCS
TraesCS2B02G535600	7.206279001	4.09242E-07	Up	3-ketoacyl-CoA synthase	KCS
TraesCS2D02G508000	9.528613227	1.83387E-13	Up	3-ketoacyl-CoA synthase	KCS
TraesCS3A02G451100	7.616757835	1.08927E-20	Up	3-ketoacyl-CoA synthase	KCS
TraesCS3B02G488500	3.834067625	4.51419E-14	Up	3-ketoacyl-CoA synthase	KCS
TraesCS6B02G372900	5.593138031	4.32531E-35	Up	3-ketoacyl-CoA synthase	KCS
TraesCS6B02G373000	7.970320318	8.09061E-09	Up	3-ketoacyl-CoA synthase	KCS
TraesCS7A02G107500	5.289345448	1.53292E-60	Up	3-ketoacyl-CoA synthase	KCS
TraesCS7A02G107700	8.339117159	1.14218E-13	Up	3-ketoacyl-CoA synthase	KCS
TraesCS7A02G107900	9.811714092	8.42497E-08	Up	3-ketoacyl-CoA synthase	KCS
TraesCS7B02G006200	4.994710636	6.6821E-22	Up	3-ketoacyl-CoA synthase	KCS
TraesCS7B02G006300	4.485093757	1.69207E-10	Up	3-ketoacyl-CoA synthase	KCS
TraesCS7D02G101900	5.535140760	3.55365E-11	Up	3-ketoacyl-CoA synthase	KCS
TraesCS7D02G102000	7.929020665	3.15637E-12	Up	3-ketoacyl-CoA synthase	KCS
TraesCS7D02G102400	9.833159298	2.62447E-14	Up	3-ketoacyl-CoA synthase	KCS
TraesCS7D02G102800	10.749264580	2.74634E-17	Up	3-ketoacyl-CoA synthase	KCS
TraesCS7D02G102900	9.529683487	2.23304E-13	Up	3-ketoacyl-CoA synthase	KCS
TraesCS6A02G341400	3.752588180	2.60291E-11	Up	3-ketoacyl-CoA synthase	KCS
TraesCS6D02G321600	4.880242341	4.20044E-41	Up	3-ketoacyl-CoA synthase	KCS
TraesCS4B02G297500	1.659827173	5.04909E-51	Up	3-ketoacyl-CoA synthase	KCS
TraesCS7B02G113100	7.980262073	1.75491E-12	Up	3-ketoacyl-CoA synthase	KCS
TraesCS7D02G208800	3.543147412	0.000554796	Up	3-ketoacyl-CoA synthase	KCS
TraesCS1A02G152100	-6.084977093	9.69328E-17	Down	3-ketoacyl-CoA synthase	KCS
TraesCS1B02G169500	-4.902294827	2.98074E-85	Down	3-ketoacyl-CoA synthase	KCS
TraesCS1D02G042300	-7.976587931	1.47581E-08	Down	3-ketoacyl-CoA synthase	KCS
TraesCS1D02G148900	-6.025460554	1.40723E-75	Down	3-ketoacyl-CoA synthase	KCS
TraesCS2A02G014600	-7.746846027	1.3141E-28	Down	3-ketoacyl-CoA synthase	KCS
TraesCS4B02G278800	-3.095441182	2.82277E-09	Down	3-ketoacyl-CoA synthase	KCS
TraesCS4D02G148300	-1.690121218	1.27211E-59	Down	3-ketoacyl-CoA synthase	KCS
TraesCS4D02G277300	-3.287359973	2.5682E-11	Down	3-ketoacyl-CoA synthase	KCS
TraesCS5A02G195500	-5.666101362	6.58809E-08	Down	3-ketoacyl-CoA synthase	KCS
TraesCS5A02G309100	-7.313293630	1.2516E-06	Down	3-ketoacyl-CoA synthase	KCS
TraesCS5B02G187900	-4.098686330	4.59612E-13	Down	3-ketoacyl-CoA synthase	KCS
基因编号 Gene ID	倍数变化 log₂ (Fold Change)	较正后P值 P_adj	显著性Significant	相关功能 Related functions	基因符号 Gene symbol
TraesCS5B02G309800	-2.962880296	1.28678E-20	Down	3-ketoacyl-CoA synthase	KCS
TraesCS5D02G195500	-5.436858301	0.000895032	Down	3-ketoacyl-CoA synthase	KCS
TraesCS5D02G316100	-4.727408251	0.001148457	Down	3-ketoacyl-CoA synthase	KCS
TraesCS6A02G026800	-8.922633648	1.48463E-11	Down	3-ketoacyl-CoA synthase	KCS
TraesCS6A02G073300	-1.822213794	9.36568E-30	Down	3-ketoacyl-CoA synthase	KCS
TraesCS6A02G383700	-2.873970208	6.2082E-56	Down	3-ketoacyl-CoA synthase	KCS
TraesCS6B02G098300	-1.303177962	1.87771E-15	Down	3-ketoacyl-CoA synthase	KCS
TraesCS6B02G422900	-2.100063721	5.41215E-19	Down	3-ketoacyl-CoA synthase	KCS
TraesCS6B02G473900	-7.535973546	2.41404E-07	Down	3-ketoacyl-CoA synthase	KCS
TraesCS6D02G071600	-1.627444447	1.73782E-23	Down	3-ketoacyl-CoA synthase	KCS
TraesCS6D02G368700	-2.358722083	3.74963E-07	Down	3-ketoacyl-CoA synthase	KCS
TraesCS1B02G161300	-1.148306727	2.23176E-11	Down	3-ketoacyl-CoA thiolase	KCS
TraesCS1A02G143900	-1.156181479	3.09259E-11	Down	3-ketoacyl-CoA thiolase	KCS
TraesCS1D02G142700	-1.228580722	2.21244E-06	Down	3-ketoacyl-CoA thiolase	KCS
TraesCS7A02G206000	-4.967018962	7.6424E-07	Down	3-ketoacyl-CoA synthase	KCS
TraesCS7A02G205700	-7.953543826	1.53209E-09	Down	3-ketoacyl-CoA synthase	KCS
TraesCS7A02G206100	-2.278867816	0.000206848	Down	3-ketoacyl-CoA synthase	KCS
TraesCS7B02G113200	-1.616125020	2.02048E-05	Down	3-ketoacyl-CoA synthase	KCS
TraesCS6A02G177700	-1.448386589	8.60285E-12	Down	3-ketoacyl-CoA synthase	KCS
TraesCS6B02G207000	-3.286252891	5.30387E-50	Down	3-ketoacyl-CoA synthase	KCS
TraesCS6D02G166600	-3.676221923	4.17639E-51	Down	3-ketoacyl-CoA synthase	KCS
TraesCS7B02G254900	-2.737547187	2.18094E-50	Down	3-ketoacyl-CoA synthase	KCS
TraesCS7D02G350500	-3.225952257	2.07987E-30	Down	3-ketoacyl-CoA synthase	KCS
TraesCS3A02G075100	1.045765218	0.000137211	Up	Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase	HCD
TraesCS6B02G373100	5.540807846	4.63743E-22	Up	Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase	HCD
TraesCS3A02G075500	5.736203743	1.8631E-27	Up	Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase	HCD
TraesCS3B02G089500	2.731387544	5.93826E-19	Up	Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase	HCD
TraesCS3B02G089600	7.196206868	1.98797E-14	Up	Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase	HCD
TraesCS3D02G075500	4.043778223	5.93877E-32	Up	Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase	HCD
TraesCS3D02G075600	5.722400469	2.04529E-18	Up	Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase	HCD
TraesCS7A02G278500	3.420890096	1.94122E-07	Up	Fatty acyl-CoA reductase	FAR
TraesCS1B02G402600	5.787124891	1.30424E-19	Up	Fatty acyl-CoA reductase	FAR
TraesCS3A02G014800	8.602623235	9.60398E-11	Up	Fatty acyl-CoA reductase	FAR
TraesCS3A02G014900	1.310317928	6.37186E-30	Up	Fatty acyl-CoA reductase	FAR
TraesCSU02G101300	2.435404624	5.30694E-06	Up	Fatty acyl-CoA reductase	FAR
TraesCS4A02G304400	-3.611496691	2.7181E-05	Down	Fatty acyl-CoA reductase	FAR
TraesCS4A02G388600	-2.765997264	1.62967E-25	Down	Fatty acyl-CoA reductase	FAR
TraesCS4B02G006800	-6.076862572	2.05241E-17	Down	Fatty acyl-CoA reductase	FAR
TraesCS4B02G010100	-4.820087287	9.98576E-05	Down	Fatty acyl-CoA reductase	FAR
基因编号 Gene ID	倍数变化 log₂ (Fold Change)	较正后P值 P_adj	显著性Significant	相关功能 Related functions	基因符号 Gene symbol
TraesCS4B02G363100	-2.308515610	2.38184E-26	Down	Fatty acyl-CoA reductase	FAR
TraesCS4D02G008300	-5.163732416	8.12712E-09	Down	Fatty acyl-CoA reductase	FAR
TraesCS4D02G356300	-1.221776468	1.36062E-11	Down	Fatty acyl-CoA reductase	FAR
TraesCS5B02G238300	-3.634781009	1.80954E-13	Down	Fatty acyl-CoA reductase	FAR
TraesCS7D02G084700	-5.018268165	9.70129E-05	Down	Fatty acyl-CoA reductase	FAR
TraesCS5A02G186200	-1.274284532	6.65E-25	Down	Fatty acid desaturase, putative	CYTB5
TraesCS6D02G139700	-1.316973984	7.37E-17	Down	Fatty acid hydroxylase superfamily	CER3
TraesCS7B02G408900	-1.098001027	0.001550775	Down	Fatty acid hydroxylase superfamily	CER1
TraesCS7D02G488800	-2.219867140	1.01E-11	Down	Fatty acid hydroxylase superfamily	CER1
TraesCS1A02G150800	2.649798059	1.72E-13	Up	Fatty acid hydroxylase superfamily	CER1
TraesCS1B02G168400	5.922991840	3.49E-11	Up	Fatty acid hydroxylase superfamily	CER1
TraesCS5B02G219400	1.245192869	7.48E-11	Up	Fatty acid hydroxylase superfamily	CER1
TraesCS6B02G423900	6.897415994	9.62E-40	Up	Fatty acid hydroxylase superfamily	CER1
TraesCS1A02G150900	2.077005421	6.41E-88	Up	Fatty acid hydroxylase superfamily protein	CER1
TraesCS1B02G168500	1.380075657	4.03E-35	Up	Fatty acid hydroxylase superfamily protein	CER1
TraesCS2A02G385200	10.776613420	2.20E-17	Up	Fatty acid hydroxylase superfamily protein	CER1
TraesCS2B02G402100	4.721003825	1.76E-62	Up	Fatty acid hydroxylase superfamily protein	CER1
TraesCS2D02G381800	3.119567058	1.53E-10	Up	Fatty acid hydroxylase superfamily protein	CER1
TraesCS2D02G381900	5.874114064	1.52E-35	Up	Fatty acid hydroxylase superfamily protein	CER1
TraesCS6A02G384600	1.155171256	1.59E-12	Up	Fatty acid hydroxylase superfamily protein	CER1
TraesCS3B02G006700	1.848872969	4.59478E-40	Up	O-acyltransferase WSD1	WSD1
TraesCS3B02G011800	1.737425505	3.93049E-21	Up	O-acyltransferase WSD1	WSD1
TraesCS3B02G300200	9.254299292	2.30951E-12	Up	O-acyltransferase WSD1	WSD1
TraesCS3B02G300300	3.640886633	0.029361692	Up	O-acyltransferase WSD1	WSD1
TraesCS3D02G266900	4.272125701	0.048631502	Up	O-acyltransferase WSD1	WSD1
TraesCS3D02G267000	2.477167555	0.000327098	Up	O-acyltransferase WSD1	WSD1
TraesCS3D02G304900	1.025977042	7.61464E-14	Up	O-acyltransferase WSD1	WSD1
TraesCS5B02G495000	2.790932718	0.000480538	Up	O-acyltransferase WSD1	WSD1
TraesCS7D02G029000	4.123068357	6.84189E-66	Up	O-acyltransferase WSD1	WSD1
TraesCS1D02G393500	-1.829048691	7.41419E-13	Down	O-acyltransferase WSD1	WSD1
TraesCS2B02G007200	-1.479385821	0.013599363	Down	O-acyltransferase WSD1	WSD1
TraesCS2D02G002200	-7.694959785	5.11215E-08	Down	O-acyltransferase WSD1	WSD1
TraesCS5B02G322300	-2.303757568	3.71433E-15	Down	O-acyltransferase WSD1	WSD1
TraesCS5D02G328700	-1.173482919	8.04625E-05	Down	O-acyltransferase WSD1	WSD1
TraesCS5D02G329000	-2.199271294	9.26924E-12	Down	O-acyltransferase WSD1	WSD1
TraesCS1A02G048700	-3.999360825	1.89244E-33	Down	Lipid transfer protein	LTP
TraesCS1B02G063800	-6.071084486	0.002379028	Down	Lipid transfer protein	LTP
TraesCS1D02G050000	-8.269069378	7.89995E-14	Down	Lipid transfer protein	LTP
TraesCS2A02G161600	-2.581595157	6.92853E-37	Down	Lipid transfer protein	LTP
TraesCS2A02G477600	-7.167418470	7.6607E-09	Down	Lipid transfer protein	LTP
TraesCS2A02G477700	-5.736333772	2.1409E-53	Down	Lipid transfer protein	LTP
TraesCS2A02G517200	-1.169382785	6.85066E-07	Down	Lipid transfer protein	LTP
TraesCS2B02G187600	-3.842135003	1.98758E-40	Down	Lipid transfer protein	LTP
TraesCS2B02G500900	-5.312215479	3.82549E-36	Down	Lipid transfer protein	LTP
基因编号 Gene ID	倍数变化 log₂ (Fold Change)	较正后P值 P_adj	显著性Significant	相关功能 Related functions	基因符号 Gene symbol
TraesCS2B02G501000	-6.596344692	3.8964E-83	Down	Lipid transfer protein	LTP
TraesCS2D02G168700	-2.555532069	2.92226E-29	Down	Lipid transfer protein	LTP
TraesCS2D02G476800	-8.559782582	1.4862E-10	Down	Lipid transfer protein	LTP
TraesCS2D02G476900	-5.295332687	4.06775E-49	Down	Lipid transfer protein	LTP
TraesCS3A02G344700	-3.745939817	6.17742E-35	Down	Lipid transfer protein	LTP
TraesCS3B02G376700	-3.206527450	2.41672E-15	Down	Lipid transfer protein	LTP
TraesCS3D02G338300	-4.085066521	1.12935E-26	Down	Lipid transfer protein	LTP
TraesCS4A02G021300	-2.323662309	0.001057144	Down	Lipid transfer protein	LTP
TraesCS4A02G038400	-1.387839823	1.10958E-06	Down	Lipid transfer protein	LTP
TraesCS4A02G194400	-4.604075831	4.77977E-10	Down	Lipid transfer protein	LTP
TraesCS4B02G120900	-5.379202934	0.023309308	Down	Lipid transfer protein	LTP
TraesCS4B02G267300	-1.949879041	0.000426936	Down	Lipid transfer protein	LTP
TraesCS4B02G282200	-2.161421287	1.24332E-14	Down	Lipid transfer protein	LTP
TraesCS4B02G393300	-1.559354249	0.009560151	Down	Lipid transfer protein	LTP
TraesCS4D02G118900	-1.858288856	5.75927E-06	Down	Lipid transfer protein	LTP
TraesCS4D02G280900	-3.221299196	1.40689E-12	Down	Lipid transfer protein	LTP
TraesCS5A02G147400	-1.935611612	1.32023E-05	Down	Lipid transfer protein	LTP
TraesCS5A02G432900	-7.367425942	4.23266E-07	Down	Lipid transfer protein	LTP
TraesCS5A02G433100	-9.371150500	3.72285E-13	Down	Lipid transfer protein	LTP
TraesCS5A02G452600	-1.858547112	1.89141E-24	Down	Lipid transfer protein	LTP
TraesCS5A02G452700	-1.131362528	1.69075E-08	Down	Lipid transfer protein	LTP
TraesCS5B02G435600	-8.259476523	9.50207E-10	Down	Lipid transfer protein	LTP
TraesCS5B02G461500	-1.302576502	1.86092E-24	Down	Lipid transfer protein	LTP
TraesCS5B02G461600	-1.431433115	2.61807E-16	Down	Lipid transfer protein	LTP
TraesCS5B02G475500	-7.960226035	1.45875E-08	Down	Lipid transfer protein	LTP
TraesCS5B02G531200	-3.706582677	0.017098014	Down	Lipid transfer protein	LTP
TraesCS5D02G441000	-8.915256266	1.41401E-11	Down	Lipid transfer protein	LTP
TraesCS5D02G441100	-7.181789193	1.53221E-06	Down	Lipid transfer protein	LTP
TraesCS5D02G441300	-6.928025584	6.41884E-06	Down	Lipid transfer protein	LTP
TraesCS6A02G317403	-1.022388097	0.013458466	Down	Lipid transfer protein	LTP
TraesCS6B02G287900	-1.686686247	0.0001889	Down	Lipid transfer protein	LTP
TraesCS7B02G012800	-1.209198548	0.002039311	Down	Lipid transfer protein	LTP
TraesCS2A02G374100	2.016327129	4.05513E-42	Up	ABC transporter G family member	ABCG
TraesCS1A02G049600	1.929415020	2.82028E-53	Up	ABC transporter G family member	ABCG
TraesCS1D02G052000	1.336780895	1.55566E-26	Up	ABC transporter G family member	ABCG
TraesCS3A02G253700	3.222114018	5.61055E-06	Up	ABC transporter G family member	ABCG
TraesCS1B02G157900	1.402488596	0.003173131	Up	ABC transporter G family-like	ABCG
TraesCS4A02G032900	2.837976707	6.35819E-54	Up	ABC transporter G family member	ABCG
TraesCS4B02G272400	1.868495946	1.28131E-13	Up	ABC transporter G family member	ABCG
TraesCS4D02G271500	1.837221709	1.80192E-19	Up	ABC transporter G family member	ABCG
基因编号 Gene ID	倍数变化 log₂ (Fold Change)	较正后P值 P_adj	显著性Significant	相关功能 Related functions	基因符号 Gene symbol
TraesCS5A02G249900	2.876220238	1.68877E-10	Up	ABC transporter G family member	ABCG
TraesCS5D02G257400	2.625663766	4.32492E-08	Up	ABC transporter G family member	ABCG
TraesCS5D02G551400	1.095936574	0.030956235	Up	ABC transporter G family member	ABCG
TraesCSU02G020100	1.559140871	7.14923E-26	Up	ABC transporter G family member	ABCG
TraesCS1A02G240500	-6.851488645	5.92353E-09	Down	ABC transporter G family member	ABCG
TraesCS1B02G252600	-3.029383117	6.83811E-18	Down	ABC transporter G family member	ABCG
TraesCS1D02G240600	-5.721995913	4.73081E-38	Down	ABC transporter G family member	ABCG
TraesCS2A02G574700	-5.053224917	1.66164E-56	Down	ABC transporter G family member	ABCG
TraesCS2B02G612200	-3.829770683	2.05699E-05	Down	ABC transporter G family member	ABCG
TraesCS2D02G585300	-4.601891609	1.09327E-54	Down	ABC transporter G family member	ABCG
TraesCS7A02G342000	-2.665683554	5.24071E-17	Down	ABC transporter G family member	ABCG
TraesCS7B02G240900	-3.320006268	1.62018E-18	Down	ABC transporter G family member	ABCG
TraesCS7D02G337600	-3.617121892	6.74189E-31	Down	ABC transporter G family member	ABCG
TraesCS4A02G103400	-10.068762360	1.88398E-15	Down	ABC transporter G family member	ABCG
TraesCS4B02G200600	-10.266524570	3.7264E-16	Down	ABC transporter G family member	ABCG
TraesCS4D02G201700	-10.695952710	1.12404E-17	Down	ABC transporter G family member	ABCG
TraesCS5D02G093600	-1.992772146	0.000108446	Down	ABC transporter G family member	ABCG

Table 3

Fig. 6

References 27

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基因编号 Gene ID	正向序列 Forward sequence (5'-3')	反向序列 Reverse sequence (5'-3')
TraesCS3A02G266700	GCAGGTAGCTTTGTATGGC	TCGATGAAATCCTCCAAAAGGC
TraesCS5B02G268900	GTACCTGTACCTCTGCTGCT	CGTCTCGTGTATCTCTTTGAGGAT
TraesCS2D02G015700	GGTGGCATTTGGACCAGGATTC	CAACCGAATTGTGCGCATTG
TraesCS2A02G307600	CCATGGATTTCTCTGTTGCA	CATGGATGTCGTCGAGAAAG
TraesCS4D02G148300	GTCAAGGTCGAGTACGCAGG	GCCAAGGTAGCAAAAATACACCAA
TraesCS7A02G115600	TGGTTCTCCAAGTGCCTTCAC	GCGTCAACGGCGCGAATA
TraesCS6D02G166600	AGATCGACACATTTCCGGTGG	TCAATGGATAAAAAGTGAACAGCA
TraesCS5B02G388800	CACCTGGTGCTCTACTGTC	CAGATGTAAGCCTCGGATCC
TraesCS1B02G049200	TCGCTGTGAACGACTTTCT	TATTGCGCCACTCTTTCTTG

样品 Sample	原始数据 Total reads (M)	过滤后数据 Unique mapping reads (M)	数据比对效率 Unique mapping (%)
Jimai 22 Rep1	78.82	60.76	77.1
Jimai 22 Rep2	79.34	59.08	74.5
Jimai 22 Rep3	70.18	53.02	75.5
glossy1 Rep1	91.29	64.66	70.8
glossy1 Rep2	74.26	53.74	72.4
glossy1 Rep3	73.17	52.12	71.2

Transcriptome profiling of glossy1 mutant with glossy glume in common wheat (Triticum aestivum L.)

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