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Acta Agronomica Sinica ›› 2022, Vol. 48 ›› Issue (6): 1389-1400.doi: 10.3724/SP.J.1006.2022.12035

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Phenotypic characterization and gene mapping of an early senescence leaf H5(esl-H5) mutant in rice (Oryza sativa L.)

ZHENG Chong-Ke1(), ZHOU Guan-Hua1, NIU Shu-Lin1,2, HE Ya-Nan1, SUN wei1, XIE Xian-Zhi1,*()   

  1. 1Shandong Rice Research Institute / Shandong Academy of Agricultural Sciences, Jinan 250100 Shandong, China
    2College of Life Sciences, Shandong Normal University, Jinan 250014, Shandong, China
  • Received:2021-05-27 Accepted:2021-10-19 Online:2022-06-12 Published:2021-12-05
  • Contact: XIE Xian-Zhi E-mail:zhengck1983@163.com;xzhxie2010@163.com
  • Supported by:
    Fund：The study was supported by the Strategic Priority Research Program of Chinese Academy of Sciences(XDA24030101-6)

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Abstract:

A stable mutant esl-H5 (early senescence leaf H5) was identified from the mutant library of japonica rice Huaidao 5 population induced by ethyl methane sulfonate (EMS) treatment. The mutant was normal at seedling stage. However, the lower leaves in esl-H5 mutant displayed premature senescence at about 50 days after sowing. Compared with the wild type (WT), the heading date of the esl-H5 mutant was delayed, while agronomical traits including plant height, panicle length, grain number per panicle, effective tiller numbers, and 1000-grain weight were significantly reduced. Moreover, chlorophyll content was also decreased in esl-H5 mutant. Genetic analysis indicated that the early senescence trait in esl-H5 mutant was controlled by a single recessive gene. ESL-H5 gene was localized on chromosome 1 using molecular marker. MutMap analysis further revealed that one nucleotide G to A replace occurred in the last exon of Os01g0533000 gene which encodes callose synthase. The G to A replace in the ESL-H5 introduced a premature stop codon. Phylogenetic analysis showed that ESL-H5 was homology with Arabidopsis AtGSL7 (Glucan Synthase-Like 7). At tillering stage, the contents of soluble sugar and starch were significantly increased in the leaves of the esl-H5 mutant compared with those of the WT. These results implied that the mutation of ESL-H5 affected the transport of photosynthetic products, resulting in premature leaf senescence phenotypes. The qRT-PCR analysis revealed that the expression levels of disease resistance-related genes PR1a, PR1b, PR2, PR4, PR5, and PR10 in esl-H5 mutant were higher than those in WT, which was consistent with the observation that esl-H5 mutant improved bacterial blight resistance. The present results lay the foundation for studying the roles of sugar signal in regulating rice senescence and disease resistance.

Key words: rice (Oryza sativa L.), early senescence, gene mapping, callose synthase

Table 1

Primers for qRT-PCR"

引物名称
Primer name		 正向引物序列
Forward sequence (5'-3')		 反向引物序列
Reverse sequence (5'-3')
OseEF-1α TTTCACTCTTGGTGTGAAGCAGAT GACTTCCTTCACGATTTCATCGTAA
OsESL-H5 GATTGGGCGACAGAAGTTTG CAGCCATCACAGAGACAAAAC
qPR1a TTCATCACCTGCAACTACTCG TGCATAAACACGTAGCATAGCAT
qPR1b GTGTGCGGGCACTACACG CGGCTTATAGTTGCATGTGA
qPR2 ACAACTCGGAGAAGCATCAG GAAACATAATCCTCGCCAAAGC
qPR4 TGGGACCTGAACAAAGTGAG GGATACACTTGCCACACGAG
qPR5 ATCGACGGCTACAACGTC GTGTCTTGGTGTTGTCTTCG
qPR10 CACCATCTACACCATGAAGC AGCACATCCGACTTTAGGAC
qOsWRKY23 TCCAGTTCCTCTCCCAGTTCTAA CACATTGTTCTCCTTTTCTTCCC
qOsWRKY72 CACCACAAATCACATCTACTCCG GCTGAAGGGAAGAGAGGTGAG
qOsSGR AGGGGTGGTACAACAAGCTG ATCCTGGATTTGGCAAGAAC
qOsNAP CAAGAAGCCGAACGGTTC GCTCCTTGCGGAAGATGTAG
qOsh36 GAACTACAGGAAGGGTCGGT GTTAGAGTGGAGCAGCAT
qOsl2 GCAGACAACAAATCGCCAAAT AGTATCCTGGTGCCAGTTCC
qOsl30 AACCTTTTTCTTGGAGATGATACGA TCTCCAGCAACTCTAACCAGCAT
qOsl85 TGGGCAAAGGAGTTACTGAA CTTGAACTGTAGGGGCTTGCTT
qOsl43 TGTGACAAGTGCTAATAATACATACGA ATCCTGGATTTGGCAAGAAC
qrbcS TCCGCTGAGTTTTGGCTATTT GGACTTGAGCCCTGGAAGG
qlhcA GTCTGTGGTTTGACCCGCT GAGCCGCCCGTTCTTGA
qlhcB GCTCAAGGTGAAGGAGATCAAGA ATGCGTTGTTGTTGACGGG
qrbcL CTTGGCAGCATTCCGAGTAA ACAACGGGCTCGATGTGATA
qPsaA GCGAGCAAATAAAACACCTTTC GTACCAGCTTAACGTGGGGAG
qPsbA CCCTCATTAGCAGATTCGTTTT ATGATTGTATTCCAGGCAGAGC
qpetD TAATGGTTTCTGTGCCGACG CCTAAAGTTAAGGATTTTTCAATGGG
qndhA CAAAGCGATATCCCAAAGGAAT CCGCATCGATACAACAACGTAT
qatpA GATCTCTCCAAACAGGCACAAG CGGCTCTTTCTAAAAGGCGTG

Fig. 1

Phenotypes of esl-H5 mutant A: phenotypes after being sowed for 10 d, bar: 1 cm; B: plant phenotypes at tillering stage, bar: 5 cm; C: plant phenotypes at filling stage, bar: 5 cm; D: phenotypes of upper three leaves at heading stage, bar: 5 cm; E: spike phenotypes at mature stage, bar: 1 cm; F and G: phenotypes of grain length and grain width, bar: 1 cm."

Table 2

Comparison of agronomic trains between esl-H5 mutant and wild type"

农艺性状
Agronomic trait		 esl-H5 野生型
Wild type
抽穗期 Heading date 124.20 ± 0.28** 120.80 ± 0.23
株高 Plant height (cm) 60.80 ± 0.42** 87.95 ± 0.62
分蘖数 Tiller number 5.75 ± 0.32** 11.65 ± 0.42
穗长Main panicle length (cm) 15.04 ± 0.23** 15.23 ± 0.21
每穗实粒数 Grain number per panicle 64.50 ± 2.65** 158.60 ± 4.80
一级枝梗数No. of primary branch 12.28 ± 0.25** 13.00 ± 0.37
二级枝梗数No. of secondary branch 20.08 ± 0.86** 27.00 ± 1.23
粒长 Grain length (mm) 7.82 ± 0.25 7.84 ± 0.21
粒宽Grain width (mm) 3.76 ± 0.17** 3.88 ± 0.11
千粒重 1000-grain weight (g) 20.90 ± 0.01** 27.20 ± 0.01

Fig. 2

Relative expression levels of senescence and photosynthesis related genes in WT and esl-H5 mutant A: relative expression levels of senescence related genes in WT and esl-H5 mutant; B: relative expression levels of photosynthesis related genes in WT and esl-H5 mutant."

Fig. 3

Chlorophyll content in esl -H5 mutant and wild type at seedling and tillering stages A: the chlorophyll content of the newly developed leaves after being sowed for 40 days; B: the chlorophyll content of the second leaves from the uppermost after being sowed for 40 days; C: the chlorophyll content of the newly developed leaves after being sowed for 60 days; D: the chlorophyll content of the second leaves from the uppermost after being sowed for 60 days; **: P < 0.01; *: P < 0.05."

Table 3

Separation ratio of three F2 segregation population"

杂交组合
Cross combinations		 F1表型 F1 phenotype F2表型 F2 phenotype
野生型
Wild type		 突变型
Mutant		 野生型
Wild type		 突变型
Mutant		 χ2 (3:1)测验
χ2 (3:1) text
WT/esl-H5 15 0 109 35 0.01
NPB/esl-H5 23 0 672 217 0.14
9311/esl-H5 19 0 105 31 0.24

Table 4

Information of markers displayed polymorphism between the pools"

标记名称
Marker name		 引物序列
Primer sequence (5'-3')		 使用的酶
Enzymes used
ID1-9F GTACGCAAGCGATCAAAC
ID1-9R AGTCCCGTCTAAAATTCACA
ID1-24F ATACTGGGTGTGCTCATTCT
ID1-24R TTTGTTTATTCTTGTCCGTTT
dSNP1F TGATGAAGGTAAGATTAGAGGATGAAGGAATTC EcoR І
dSNP1F TCAGTTAGAAATTACTCCCT
dSNP2F AGGGATCCGAATAGGAGTCCTACGGGCCTTGGGTACC Kpn І
dSNP2R GCACCGCAAACCAGCGTTGCCT
dSNP3F CCTGTTGTTTGTTGTTTCTTCCATCTAGA Xba І
dSNP3R GCACCAACCAGTAGGCATGA

Fig. 4

Mapping and cloning of ESL-H5 genes A: fine mapping of ESL-H5 (the marker above the horizontal line represents the InDel or SNP marker used in fine mapping, and the number below represents the number of recombinants); B: changes of G to A in the genome of Os01g0533000; C: SNP3 is converted to dCAPS marker; D: plants with early senescence phenotype was detected using dCAPS markers. E: relative expression level of Os01g0533000 in WT and esl-H5."

Fig. 5

Protein structure prediction and phylogenetic tree analysis of ESL-H5 A: protein structure prediction of wild-type and esl-H5 mutant, VTA1, FKS1, and Glucan synthase refer to protein domains; B: ESL-H5 transmembrane region prediction; C: phylogenetic tree analysis of callose synthase in Arabidopsis and rice; D: alignment of amino acid sequences of ESL-H5 and AtGSL7."

Fig. 6

Relative expression patterns of ESL-H5 genes"

Fig. 7

Starch and soluble sugar contents in wild type and mutant leaves at tillering stage A: soluble sugar contents at 6:00; B: soluble sugar contents at 18:00; C: starch contents at 6:00; D: starch contents at 18:00. *: P < 0.05; **: P < 0.01."

Fig. 8

Identification of resistance to bacterial blight of wild type and mutant at tillering stage A: phenotype of bacterial blight resistance; B: statistics of lesion length; C: relative expression levels of pathogenesis-related protein. **: P < 0.01."

[1] Leister D. Chloroplast research in the genomic age. Trends Genet, 2003, 19: 47-56.
pmid: 12493248
[2] Zheng Y H, Xu J M, Wang F J, Tang Y C, Wei Z, Ji Z Y, Wang C L, Zhao K J. Mutation types of cyp71p1 cause different phenotypes of mosaic spot lesion and premature leaf senescence in rice. Front Plant Sci, 2021, 12: 641300.
doi: 10.3389/fpls.2021.641300
[3] Schippers J H, Schmidt R, Wagstaff C, Jing H C. Living to die and dying to live: the survival strategy behind leaf senescence. Plant Physiol, 2015, 169: 914-930.
doi: 10.1104/pp.15.00498 pmid: 26276844
[4] Wingler A. Transitioning to the next phase: the role of sugar signaling throughout the plant life cycle. Plant Physiol, 2018, 176: 1075-1084.
doi: 10.1104/pp.17.01229 pmid: 28974627
[5] Dai N, Schaffer A, Petreikov M, Shahak Y, Giller Y, Ratner K, Levine A, Granot D. Overexpression of Arabidopsis hexokinase in tomato plants inhibits growth, reduces photosynthesis, and induces rapid senescence. Plant Cell, 1999, 11: 1253-1266.
pmid: 10402427
[6] Quirino B F, Noh Y S, Himelblau E, Amasino R M. Molecular aspects of leaf senescence. Trends Plant Sci, 2000, 5: 278-282.
pmid: 10871899
[7] Wingler A, Delatte T L, O’Hara L E, Primavesi L F, Jhurreea D, Paul M J, Schluepmann H. Trehalose 6-phosphate is required for the onset of leaf senescence associated with high carbon availability. Plant Physiol, 2012, 158: 1241-1251.
doi: 10.1104/pp.111.191908 pmid: 22247267
[8] Baena-González E, Sheen J. Convergent energy and stress signaling. Trends Plant Sci, 2008, 13: 474-482.
doi: 10.1016/j.tplants.2008.06.006 pmid: 18701338
[9] Liang C, Hirose T, Okamura M, Tanimoto R, Miyao A, Hirochika H, Terao T, Li T, Ohsugi R, Aoki N. Phenotypic analyses of rice lse2 and lse3 mutants that exhibit hyperaccumulation of starch in the leaf blades. Rice, 2014, 7: 32.
doi: 10.1186/s12284-014-0032-3
[10] 肖艳华, 何光华, 陈新龙, 杜丹, 邢亚迪, 张天泉, 祝毛迪, 刘明明, 朱小燕, 桑贤春. 水稻叶片淀粉积累及早衰突变体esl9的鉴定与基因定位. 作物学报, 2017, 43: 473-482.
doi: 0.3724/SP.J.1006.2017.00473
Xiao Y H, He G H, Chen X L, Du D, Xing Y D, Zhang T Q, Zhu M D, Liu M M, Zhu X Y, Sang X C. Identification and gene mapping of starch accumulation and early senescence leaf mutant esl9 in rice. Acta Agron Sin, 2017, 43: 473-482 (in Chinese with English abstract).
[11] Chen X L, Zhu M D, Gu F X, Liu M M, Zhang Y Y, Xing Y D, Du D, Xiao Y H, Zhu X Y, He G H. Identification and gene fine mapping of starch accumulation and early senescent leaf mutant esl10 in rice. Crop Sci, 2018, 58: 204-217.
doi: 10.2135/cropsci2017.05.0323
[12] Huang J, Yan M, Zhu X, Zhang T, Shen W Q, Yu P, Wang Y, Sang X, Yu G, Zhao B. Gene mapping of starch accumulation and premature leaf senescence in the ossac3 mutant of rice. Euphytica, 2018, 214: 177.
doi: 10.1007/s10681-018-2261-9
[13] 林添资, 孙立亭, 龚红兵, 刘玲珑, 赵志刚, 董慧, 王益华, 江玲, 万建民. 水稻叶片淀粉累积早衰突变体pls5的鉴定及基因定位. 南京农业大学学报, 2020, 43: 414-422.
Lin T Z, Sun L T, Gong H B, Liu L L, Zhao Z G, Dong H, Wang Y H, Jiang L, Wan J M. Identification and gene mapping of a premature leaf senescence 5 mutant with starch accumulation in rice leaves. J Nanjing Agric Univ, 2020, 43: 414-422 (in Chinese with English abstract).
[14] Zhu M D, Chen X L, Zhu X Y, Xing Y D, Zhang T Q. Identification and gene mapping of the starch accumulation and premature leaf senescence mutant ossac4 in rice. J Integr Agric, 2020, 19: 2150-2164.
doi: 10.1016/S2095-3119(19)62814-5
[15] Arnon D I. Copper enzymes in isolated chloroplasts. Polyphenoloxidase in beta vulgaris. Plant Physiol, 1949, 24: 1-15.
doi: 10.1104/pp.24.1.1 pmid: 16654194
[16] 邹琦. 植物生理学实验指导. 北京: 中国农业出版社, 2003. pp 160-161.
Zou Q. Plant Physiology Experiment. Beijing: China Agriculture Press, 2003. pp 160-161(in Chinese).
[17] Rogers S O, Bendich A J. Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues. Plant Mol Biol, 1985, 5: 69-76.
doi: 10.1007/BF00020088 pmid: 24306565
[18] Takagi H, Uemura A, Yaegashi H, Tamiru M, Abe A, Mitsuoka C, Utsushi H, Natsume S, Kanzaki H, Matsumura H. Mutmap-gap: whole-genome resequencing of mutant f2 progeny bulk combined with de novo assembly of gap regions identifies the rice blast resistance gene pii. New Phytol, 2013, 200: 276-283.
doi: 10.1111/nph.2013.200.issue-1
[19] Zheng C K, Zhou J J, Zhang F, Yin J J, Zhou G H, Li Y P, Chen F, Xie X Z. OsABAR1, a novel GRAM domain-containing protein, confers drought and salt tolerance via an ABA-dependent pathway in rice. Plant Physiol Biochem, 2020, 152: 138-146.
doi: 10.1016/j.plaphy.2020.04.032
[20] 王春连, 赵炳宇, 章琦, 赵开军, 邢全党. 水稻白叶枯病新抗源y238的鉴定及其近等基因系培育. 植物遗传资源学报, 2004, 5: 26-30.
Wang C L, Zhao B Y, Zhang Q, Zhao K J, Xing Q D. Identification of a new rice germplasm with resistance to bacterial blight and the breeding of a near-isogenic line. J Plant Genet Resour, 2004, 5: 26-30 (in Chinese with English abstract).
[21] Barratt D H, Kölling K, Graf A, Pike M, Calder G, Findlay K, Zeeman S C, Smith A M. Callose synthase GSL7 is necessary for normal phloem transport and inflorescence growth in Arabidopsis. Plant Physiol, 2011, 155: 328-341.
doi: 10.1104/pp.110.166330 pmid: 21098675
[22] Xie B, Wang X, Zhu M, Zhang Z, Hong Z. Cals7 encodes a callose synthase responsible for callose deposition in the phloem. Plant J, 2011, 65: 1-14.
doi: 10.1111/j.1365-313X.2010.04399.x
[23] Moghaddam M R B, Van den Ende W. Sugars and plant innate immunity. J Exp Bot, 2012, 63: 3989-3998.
doi: 10.1093/jxb/ers129 pmid: 22553288
[24] 何丹, 姜鸿瑞, 叶亚峰, 杨阳, 任艳, 陶亮之, 吴跃进, 刘斌美. 水稻早衰快腐突变体ad1衰老特性的研究. 核农学报, 2021, 35: 769-779.
He D, Jiang H R, Ye Y F, Yang Y, Ren Y, Tao L Z, Wu Y J, Liu B M. Analysis of the mechanism of early senescence degeneration in rice mutant ad1. J Nucl Agric Sci, 2021, 35: 769-779.
[25] Hong Z, Zhang Z, Olson J M, Verma D P. A novel UDP-glucose transferase is part of the callose synthase complex and interacts with phragmoplastin at the forming cell plate. Plant Cell, 2001, 13: 769-779.
pmid: 11283335
[26] Vatén A, Dettmer J, Wu S, Stierhof Y D, Miyashima S, Yadav S R, Roberts C J, Campilho A, Bulone V, Lichtenberger R, Lehesranta S, Mähönen A P, Kim J Y, Jokitalo E, Sauer N, Scheres B, Nakajima K, Carlsbecker A, Kimberly L G, Helariutta Y. Callose biosynthesis regulates symplastic trafficking during root development. Dev Cell, 2011, 21: 1144-1155.
doi: 10.1016/j.devcel.2011.10.006 pmid: 22172675
[27] Dong X, Hong Z, Chatterjee J, Kim S, Verma D P. Expression of callose synthase genes and its connection with Npr1 signaling pathway during pathogen infection. Planta, 2008, 229: 87-98.
doi: 10.1007/s00425-008-0812-3
[28] Xie B, Wang X, Hong Z. Precocious pollen germination in Arabidopsis plants with altered callose deposition during microsporogenesis. Planta, 2010, 231: 809-823.
doi: 10.1007/s00425-009-1091-3 pmid: 20039178
[29] Huang L, Chen X Y, Rim Y, Han X, Cho W K, Kim S W, Kim J Y. Arabidopsis glucan synthase-like 10 functions in male gametogenesis. J Plant Physiol, 2009, 166: 344-352.
doi: 10.1016/j.jplph.2008.06.010
[30] Nishikawa S, Zinkl G M, Swanson R J, Maruyama D, Preuss D. Callose (beta-1,3 glucan) is essential for Arabidopsis pollen wall patterning, but not tube growth. BMC Plant Biol, 2005, 5: 22.
doi: 10.1186/1471-2229-5-22
[31] Töller A, Brownfield L, Neu C, Twell D, Schulze-Lefert P. Dual function of Arabidopsis glucan synthase-like genes GSL8 and GSL10 in male gametophyte development and plant growth. Plant J, 2008, 54: 911-923.
doi: 10.1111/j.1365-313X.2008.03462.x
[32] Dong X, Hong Z, Sivaramakrishnan M, Mahfouz M, Verma D P. Callose synthase (Cals5) is required for exine formation during microgametogenesis and for pollen viability in Arabidopsis. Plant J, 2005, 42: 315-328.
doi: 10.1111/tpj.2005.42.issue-3
[33] Enns L C, Kanaoka M M, Torii K U, Comai L, Okada K, Cleland R E. Two callose synthases, GSL1 and GSL5, play an essential and redundant role in plant and pollen development and in fertility. Plant Mol Biol, 2005, 58: 333-349.
doi: 10.1007/s11103-005-4526-7
[34] Jacobs A K, Lipka V, Burton R A, Panstruga R, Strizhov N, Schulze-Lefert P, Fincher G B. An Arabidopsis callose synthase, is required for wound and papillary callose formation. Plant Cell, 2003, 15: 2503-2513.
doi: 10.1105/tpc.016097
[35] Shi X, Sun X H, Zhang Z G, Feng D, Zhang Q, Han L D, Wu J X, Lu T G. Glucan synthase-like 5 (GSL5) plays an essential role in male fertility by regulating callose metabolism during microsporogenesis in rice. Plant Cell Physiol, 2015, 56: 497-509.
doi: 10.1093/pcp/pcu193
[36] Song L Z, Wang R C, Zhang L, Wang Y M, Yao S G. CRR1 encoding callose synthase functions in ovary expansion by affecting vascular cell patterning in rice. Plant Physiol, 2016, 88: 620-632.
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