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Acta Agronomica Sinica ›› 2022, Vol. 48 ›› Issue (11): 2715-2723.doi: 10.3724/SP.J.1006.2022.12040

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Mapping of QTLs for resistance to white-backed planthopper in Guangxi wild rice Y11

YANG Ming1(), LI Dan-Ting2, FAN De-Jia1, TAN Song-Juan1, CHENG Xia-Nian1, LIU Yu-Qiang1,*(), WAN Jian-Min1,3   

  1. 1State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China
    2Rice Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, Jiangsu, China
    3Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2021-06-06 Accepted:2022-05-05 Online:2022-11-12 Published:2022-05-24
  • Contact: LIU Yu-Qiang E-mail:2018101087@njau.edu.cn;yql@njau.edu.cn
  • Supported by:
    The National Key Research and Development Program of China(2017YFD0100400-01);The National Natural Science Foundation of China(32088102);The National Natural Science Foundation of China(32072030);The Jiangsu Provincial Key Research Program(BE2019380)

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Abstract:

White-backed planthopper (WBPH) is one of the most serious pests in rice production. Breeding resistant varieties is the most economical and effective strategy to control the white-backed planthopper. In this study, we found that Guangxi wild rice variety ‘Y11’ had high resistance to white-backed planthopper. The number and survival rate of WBPH were significantly less than the susceptible variety ‘Guanghui 998’. These results showed that ‘Y11’ displayed higher antixenosis and antibiosis against WBPH. Subsequently, ‘Y11’ was as the donor parent and an indica cultivar ‘Guanghui 998’ was as the recurrent parent by continuous backcrossing and selfing to obtain the BC3F9 population. Furthermore, to identify the quantitative trait locus (QTL) for white-backed planthopper resistance, we completed the evaluation of this population for WBPH resistance at seedling stage and constructed the genome-wide linkage map. Three QTLs were detected on the three different rice chromosomes, and designed as qWBPH2Y11, qWBPH6Y11, and qWBPH11Y11, respectively. The LOD values were 4.8, 2.5, and 3.7, which accounted for 9.3%, 2.3%, and 5.6% of the phenotypic variation, respectively. All of the resistance alleles were from resistant parent ‘Y11’. Plant harboring qWBPH2 Y11 was selected from BC3F9 population to continuously backcross with ‘Guanghui 998’. The near isogenic line of qWBPH2 Y11 was developed by molecular marker assisted selection, which displayed higher resistance to WBPH than the background parent ‘Guanghui 998’. The mapping of WBPH resistance QTLs and the development of the near isogenic line in this study will be helpful for cloning resistance genes and breeding resistant in rice cultivars.

Key words: rice (Oryza sativa L.), white-backed planthopper (WBPH), QTLs, gene mapping

Table 1

Evaluation criteria for rice resistant against white-backed planthopper"

等级
Resistance score		 死苗率
Mortality rate (%)		 受害症状
Damage symptoms
0 <1.0 无损伤
No damage
1 1.1-10.0 非常微弱损伤
Very weak damage
3 10.1-30.0 第一、二叶叶尖发黄, 轻微的发育迟缓
The first and second leaves were yellow tips and slight developmental delay
5 30.1-50.0 明显的黄化矮化或大约一半的植物枯萎或死亡
Significant yellowing dwarfing or about half of the plants withered or died
7 50.1-70.0 超过半数植株死亡, 其他植株明显矮化萎蔫
More than half of the plants died, and the other plants were obviously dwarfed and wilted
9 >70.0 所有植株死亡
All the plants died

Fig. 1

Comparison of the resistance against WBPH between ‘Y11’ and ‘Guanghui 998’ A: ‘Y11’ and ‘Guanghui 998’ on 10 days post infestation with WBPH; B: seeding mortality rate of ‘Y11’ and ‘Guanghui 998’ on 10 days post infestation with WBPH; C: survival rate of WBPH on the plants of ‘Y11’ and ‘Guanghui 998’ on 1, 2, and 4 day(s) post infestation with WBPH, respectively; D: the number of WBPH on ‘Y11’ and ‘Guanghui 998’ on 0-, 1-, 2-, 3-, and 4-day post infestation with 20 nymphs per plant, respectively. E: the number of WBPH on ‘Y11’ and ‘Guanghui 998’ surrounding the plant with heavily infested with WBPH at 1, 2, 4, 6, 12, 24, and 48 h. *: P < 0.05; **: P < 0.01. Bar: 3 cm."

Fig. 2

Frequency distribution of the WBPH-resistance score in ‘Y11’ / ‘Guanghui 998’ BC3F9 population"

Fig. 3

Molecular genetic map of ‘Y11’ / ‘Guanghui 998’ BC3F9 population"

Fig. 4

Chromosomal location of QTLs for WBPH resistance The black oval indicates the position of the QTLs on the chromosomes."

Table 2

QTLs for WBPH resistance detected in ‘Y11’/‘Guanghui 998’ BC3F9 population"

QTL 染色体
Chr.		 标记区间
Marker interval		 LOD值
LOD score		 贡献率
Variance explained (%)		 加性效应
Additive effect
qWBPH2Y11 2 id2013634 to id2015636 4.8 9.3 -0.20
qWBPH6Y11 6 id6006868 to id6007754 2.5 2.3 -0.07
qWBPH11Y11 11 id11005456 to K_id11007840 3.7 5.6 -0.17

Fig. 5

WBPH resistance of the near isogenic lines carrying qWBPH2Y11 A: ‘W0394’ and ‘Guanghui 998’ on 10 days post infestation with WBPH; B: seeding mortality rate of ‘W0394’ and ‘Guanghui 998’ on 10 days post infestation with WBPH. Bar: 3 cm; **: P < 0.01."

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