蚕豆SSR标记遗传多样性及与淀粉含量的关联分析

doi:10.3724/SP.J.1006.2022.14190

Abstract

Abstract:

Starch is the most abundant type of carbohydrate in faba bean grain. In general, starch content affects its physical and chemical properties, thereby affecting the quality and the use of faba bean. Understanding the genetic diversity of faba bean varieties and finding molecular markers closely linked to faba bean starch content are of great significance to the molecular marker-assisted (MAS) breeding of faba bean. In this study, 260 accessions of faba bean germplasm were used as test materials to determine the total starch content, amylose content and amylopectin content in 2019 and 2020. To analyze the genetic diversity and population genetic structure of 260 accessions of faba bean materials, the GLM and MLM models of Tassel 2.1 software were used to analyze the association between markers and starch content using 132 pairs of SSR markers with significant polymorphism. The results showed that the coefficient of phenotypic variation ranged from 13.21% to 20.52% with an average value of 14.85%, indicating that the test population had a certain phenotypic diversity. A total of 629 polymorphic locus were detected by 132 markers in 260 accessions of faba bean materials. The average number of alleles at each site was 4.77, and the range of variation was 2-11. The value of polymorphism information (PIC) was between 0.0385 and 0.7772, with an average of 0.4935. About 50% of the PIC values were greater than the average, indicating that the diversity of markers used in this study was relatively high. Cluster analysis divided 260 materials into three groups. Group I included 42 materials, Group II included 59 materials, and Group III included 159 materials. The classification results reflected the genetic relationship between faba bean varieties. The population genetic structure analysis divided 260 materials into two subgroups, indicating that the test population structure was relatively simple, which was conducive to association analysis. Based on the GLM model and the MLM model, eight markers significantly (P < 0.01) associated with the content of total starch, amylose and amylopectin were detected in two years respectively. The explanation rates of single markers for phenotypic variation were 4.54%-9.04% and 2.69%-10.39%, respectively. The SSR-10927 marker was significantly (P < 0.05) associated with amylopectin content in two years and in both models, and was significantly (P < 0.05) associated with the total starch content in the two models in 2019 and the MLM model in 2020 with the highest detection rate. The results of this study laid a theoretical foundation for faba bean MAS breeding and the selection of parental materials.

Key words: faba bean, SSR marker, starch content, genetic diversity, association analysis

LIU Yu-Ling, ZHANG Hong-Yan, TENG Chang-Cai, ZHOU Xian-Li, HOU Wan-Wei. Genetic diversity and its association analysis of SSR markers with starch content in faba bean (Vicia faba L.)[J].Acta Agronomica Sinica, 2022, 48(11): 2786-2796.

Figures/Tables 9

Table S1

Variety information of 260 faba bean materials"

序号 Serial number	全国统一编号 Unified national numbering	品种名 Variety name	来源地 Source place
1	H0000002	蚕豆 Faba bean	中国山西 Shanxi, China
2	H0000006	大马牙 Damaya	中国山西 Shanxi, China
3	H0000015	洋蚕豆Yang faba bean	中国山西 Shanxi, China
4	H0000016	家蚕豆Jia faba bean	中国山西 Shanxi, China
5	H0000039	白马牙 Baimaya	中国山西 Shanxi, China
6	H0000090	栗豆 Chestnut beans	中国内蒙古 Inner Mongolia, China
7	H0000091	红蚕豆 Red faba bean	中国内蒙古 Inner Mongolia, China
8	H0000095	当地树豆 Local tree beans	中国内蒙古 Inner Mongolia, China
9	H0000109	牛踏扁 Niutabian	中国江苏 Jiangsu, China
10	H0000110	青皮 Green husk	中国江苏 Jiangsu, China
11	H0000113	牛踏扁 Niutabian	中国浙江 Zhejiang, China
12	H0000116	小粒长荚 Small seeds and long pods	中国浙江 Zhejiang, China
13	H0000117	翠湾白皮豆 Cuiwanbaipi bean	中国浙江 Zhejiang, China
14	H0000138	川黄小粒种 Chuanhuan Small grain species	中国浙江 Zhejiang, China
15	H0000152	青皮大脚板 Qingpidajiaoban	中国湖北 Hubei, China
16	Q15	湟源马牙 HuangyuanMaya	中国青海 Qinghai, China
17	H0000158	胡豆 faba bean	中国湖北 Hubei, China
18	H0000167	白蚕豆 White faba bean	中国云南 Yunnan, China
19	H0000172	拉市豆 Lashi bean	中国云南 Yunnan, China
20	H0000184	绿皮豆 Green-skinned bean	中国云南 Yunnan, China
21	H0000189	宝峰蚕豆 Baofeng faba bean	中国云南 Yunnan, China
22	H0000190	新街蚕豆 Xinjie faba bean	中国云南 Yunnan, China
23	H0000191	夕阳大蚕豆 Sunset big faba bean	中国云南 Yunnan, China
24	H0000193	小红皮蚕豆 Little red-skinned faba bean	中国云南 Yunnan, China
25	H0000195	莲峰蚕豆 Lianfeng faba bean	中国云南 Yunnan, China
26	H0000200	马街蚕豆 Majie faba bean	中国云南 Yunnan, China
27	H0000201	三岔河蚕豆 Sanchahe faba bean	中国云南 Yunnan, China
28	H0000203	绿叶豆 Green leaves bean	中国云南 Yunnan, China
29	H0000207	大同甫豆 Datongfu bean	中国云南 Yunnan, China
30	H0000216	中粒豆 Medium grain bean	中国云南 Yunnan, China
31	H0000218	细蚕豆 Fine faba bean	中国云南 Yunnan, China
32	H0000242	洱源豆 Eryuan bean	中国云南 Yunnan, China
33	H0000244	大蚕豆 Big faba bean	中国云南 Yunnan, China
34	H0000245	蚕豆 Faba bean	中国云南 Yunnan, China
35	H0000248	大红豆 Big red bean	中国云南 Yunnan, China
36	H0000285	青海五号 Qinghai No.5	中国青海 Qinghai, China
37	H0000308	高产地大粒 High yield and large grain	叙利亚 Syria
38	H0000334	74TA.12	叙利亚 Syria
39	H0000341	23856	叙利亚 Syria
40	H0000360	Syr.L.L	叙利亚 Syria
41	H0000363	高产地 High yield	叙利亚 Syria
42	H0000364	Syr.L.M	叙利亚 Syria
43	H0000365	Syr.L.M	叙利亚 Syria
44	H0000376	23794	叙利亚 Syria
45	H0000389	23705	叙利亚 Syria
46	H0000423	23925	叙利亚 Syria
47	Q13	透心绿 Through Heart Green	中国青海 Qinghai, China
48	H0000432	79S 97514	伊拉克 Iraq
49	H0000435	76TA56297	伊拉克 Iraq
50	H0000438	74TA374	黎巴Lebanon
51	Q12	羊眼豆 Sheep Eyed Peas	中国青海 Qinghai, China
52	H0000456	New Mammoth	英国 UK
53	H0000460	78S 33120×75TA43	英国 UK
54	H0000492	8062	叙利亚 Syria
55	H0000508	8008	伊拉克 Iraq
56	H0000509	7011	伊拉克 Iraq
57	H0000513	7016	伊拉克 Iraq
58	H0000518	8060	黎巴嫩 Lebanon
59	H0000539	8077	埃及 Egypt
60	H0000541	7033	阿尔及利亚 Algeria
61	H0000549	7045	西班牙 Spain
62	H0000552	8055	西班牙 Spain
63	H0000570	7047	英国 Britain
64	H0000577	8033	乌拉圭 Uruguay
65	H0000578	7003	约旦 Jordan
66	H0000605	M5179	民主德国 Former German Democratic Republic
67	H0000607	63180	民主德国 Former German Democratic Republic
68	H0000693	237	土耳其 Turkey
69	H0000698	944	阿富汗Afghanistan
70	H0000701	23	伊拉克 Iraq
71	H0000782	427	苏丹 The Sudan
72	H0000802	369	阿尔及利亚 Algeria
73	H0000805	372	阿尔及利亚 Algeria
74	H0000807	816	阿尔及利亚 Algeria
75	H0000809	402	突尼斯 Tunisia
76	H0000811	922	突尼斯 Tunisia
77	H0000813	926	突尼斯 Tunisia
78	H0000815	928	突尼斯 Tunisia
79	H0000825	100	西班牙 Spain
80	H0000876	972	波兰 Poland
81	H0000918	大豆 soybean	中国山西 Shanxi, China
82	H0000930	大马牙 Damaya	中国山西 Shanxi, China
83	H0001019	句容蚕豆 Jurong faba bean	中国江苏 Jiangsu, China
84	H0001033	海安白皮 Haian white skin	中国江苏 Jiangsu, China
85	H0001034	丹徒大粒 Dantu big grain	中国江苏 Jiangsu, China
86	H0001035	丹徒小粒 Dantu small grain	中国江苏 Jiangsu, China
87	H0001037	句容白皮 Jurong white skin	中国江苏 Jiangsu, China
88	H0001039	南郭豆 Nanguo bean	中国江苏 Jiangsu, China
89	H0001075	黄皮田鸡青 Yellow skin Tianjiqing	中国浙江Zhejiang, China
90	H0001080	黄皮细叶青 Yellow skin Xiyeqing	中国浙江Zhejiang, China
91	H0001082	本地种 Native species	中国浙江Zhejiang, China
92	H0001085	宁波川 Ningbochuan	中国浙江Zhejiang, China
93	H0001086	中籽 Zhongzi	中国浙江Zhejiang, China
94	H0001091	池槐豆 Chihuai bean	中国浙江Zhejiang, China
95	H0001105	石门青 Shimenqing	中国浙江Zhejiang, China
96	H0001108	小粒豆 Small grain bean	中国浙江Zhejiang, China
97	H0001109	新塘青豆 Qingtang green beans	中国浙江Zhejiang, China
98	H0001112	本地三月黄 Local March yellow	中国浙江Zhejiang, China
99	H0001114	阔板青 Kuobanqing	中国浙江Zhejiang, China
100	H0001290	小粒佛豆 Small grain Buddha bean	中国浙江Zhejiang, China
101	H0001346	大青片 Daqingpian	中国安徽 Anhui, China
102	H0001381	青稞蚕豆 Highland barley faba bean	中国湖北 Hubei, China
103	H0001392	蚕豆 Faba bean	中国江西 Jiangxi, China
104	H0001402	蚕豆 Faba bean	中国江西 Jiangxi, China
105	H0001407	蚕豆 Faba bean	中国江西 Jiangxi, China
106	H0001431	大棵蚕豆 Dake faba bean	中国湖北 Hubei, China
107	H0001432	鞋板豆 Xieban bean	中国湖北 Hubei, China
108	H0001433	小板粒蚕豆 Small plate grain faba bean	中国湖北 Hubei, China
109	H0001438	蚕豆 Faba bean	中国湖南 Hunan, China
110	H0001600	蚕豆 Faba bean	中国湖南 Hunan, China
111	H0001602	蚕豆 Faba bean	中国广西 Guangxi, China
112	H0001624	大白胡豆 Dabai faba bean	中国四川 Sichuan, China
113	H0001629	大胡豆 Big faba bean	中国四川 Sichuan, China
114	H0001633	二板豆 Erban bean	中国四川 Sichuan, China
115	H0001635	大白胡豆 Dabai faba bean	中国四川 Sichuan, China
116	H0001637	二饭早 Erfanzao	中国四川 Sichuan, China
117	H0001645	二青胡豆 Erqing faba bean	中国四川 Sichuan, China
118	H0001652	青胡豆 Qing faba bean	中国四川 Sichuan, China
119	H0001667	二白胡豆 Erbai faba bean	中国四川 Sichuan, China
120	H0001668	大白胡豆 Dabai faba bean	中国四川 Sichuan, China
121	H0001669	铁夹胡豆 Tiejia faba bean	中国四川 Sichuan, China
122	H0001671	大白胡豆 Dabai faba bean	中国四川 Sichuan, China
123	H0001693	水胡豆 Water faba bean	中国四川 Sichuan, China
124	H0001706	红胡豆 Red faba bean	中国四川 Sichuan, China
125	H0001718	棉花胡豆 Cotton faba bean	中国贵州 Guizhou, China
126	H0001722	细米胡豆 Fine-grained faba bean	中国贵州 Guizhou, China
127	H0001724	大胡豆 Big faba bean	中国贵州 Guizhou, China
128	H0001731	二胡豆 Er faba bean	中国贵州 Guizhou, China
129	H0001737	大胡豆 Big faba bean	中国贵州 Guizhou, China
130	H0001758	细米胡豆 Fine-grained faba bean	中国贵州 Guizhou, China
131	H0001760	大白胡豆 Dabai faba bean	中国贵州 Guizhou, China
132	H0001761	山宝白皮 Shanbao White skin	中国云南 Yunnan, China
133	H0001773	蚕豆 Faba bean	中国云南 Yunnan, China
134	H0001775	清河蚕豆 Qinghe faba bean	中国云南 Yunnan, China
135	H0001776	研和蚕豆 Yanhe faba bean	中国云南Yunnan, China
136	H0001777	葫芦冲豆 Huluchong bean	中国云南Yunnan, China
137	H0001780	撮科豆 Cuoke bean	中国云南Yunnan, China
138	H0001781	草庆豆 Caoqing bean	中国云南Yunnan, China
139	H0001784	龙子大白豆LongziDabai bean	中国云南 Yunnan China
140	H0001791	江川绿叶豆 Jiangchuan green leaves bean	中国云南Yunnan, China
141	H0001796	半大豆 Semi-soybean	中国云南Yunnan, China
142	H0001797	腊古蚕豆 Nagu faba bean	中国云南Yunnan, China
143	H0001802	胡豆 faba bean	中国云南Yunnan, China
144	H0001805	胡豆 faba bean	中国陕西 Shaanxi, China
145	H0001806	老胡豆 Old faba bean	中国陕西 Shaanxi, China
146	H0001808	米胡豆 Mi faba bean	中国陕西 Shaanxi, China
147	H0001854	康乐牛踏扁KangleNiutabian	中国陕西 Shaanxi, China
148	H0001855	临潭蚕豆 Lintan faba bean	中国甘肃 Gansu, China
149	H0001859	文县蚕豆 Wenxian faba bean	中国甘肃 Gansu, China
150	H0001863	岷县蚕豆 Minxian faba bean	中国甘肃 Gansu, China
151	H0001864	武山蚕豆 Wushan faba bean	中国甘肃 Gansu, China
152	H0001865	漳县老大豆ZhangxianLao soybean	中国甘肃 Gansu, China
153	H0001877	民勤蚕豆 Minqin faba bean	中国甘肃 Gansu, China
154	H0001881	民乐洪水蚕豆 Minle flood faba Bean	中国甘肃 Gansu, China
155	H0001884	临洮红蚕豆 Lintao red faba bean	中国甘肃 Gansu, China
156	H0001897	马牙蚕豆 Maya faba bean	中国青海 Qinghai, China
157	H0001900	红大豆 Red soybean	中国青海 Qinghai, China
158	H0001935	西皮尕大豆 Xipiga soybean	中国青海 Qinghai, China
159	H0001939	曹家堡尕大豆 Caojiabaoga soybean	中国青海 Qinghai, China
160	H0001940	尕马牙 Gamaya	中国青海 Qinghai, China
161	H0001947	蚕豆 Faba bean	中国宁夏 Ningxia, China
162	H0001953	蚕豆 Faba bean	中国宁夏 Ningxia, China
163	H0001955	25	伊拉克 Iraq
164	H0002297	281	黎巴嫩 Lebanon
165	H0002308	285	黎巴嫩 Lebanon
166	H0002311	323	约旦 Jordan
167	H0002314	638	加拿大 Canada
168	H0002332	590	加拿大 Canada
169	H0002368	616	加拿大 Canada
170	H0002375	617	加拿大 Canada
171	H0002376	508	加拿大 Canada
172	H0002382	817	法国 France
173	H0002409	800469	德国 Germany
174	H0002414	MNRISBEAGUE	德国 Germany
175	H0002431	780011	德国 Germany
176	H0002432	STRUBE	德国 Germany
177	H0002437	159	希腊 Greece
178	H0002469	171	荷兰 Netherlands
179	H0002470	166	荷兰 Netherlands
180	H0002473	247	匈牙利 Hungary
181	H0002474	259	匈牙利 Hungary
182	H0002475	876	黎巴嫩 Lebanon
183	H0002492	493	阿富汗 Afghanistan
184	H0002503	595	加拿大 Canada
185	H0002505	615	加拿大 Canada
186	H0002515	150	前苏联 Former Soviet Union
187	H0002516	804	阿富汗 Afghanistan
188	H0002517	成胡1号 Chenghu No.1	中国四川 Sichuan, China
189	H0002527	成胡10号 Chenghu No.10	中国四川 Sichuan, China
190	H0002531	大青皮蚕豆 Big green husk faba bean	中国江苏 Jiangsu, China
191	H0002542	绿蚕豆 Green faba bean	中国宁夏 Ningxia, China
192	H0002621	紫蚕豆 Purple faba bean	中国宁夏 Ningxia, China
193	H0002622	蚕豆 Faba bean	中国宁夏 Ningxia, China
194	H0002693	蚕豆 Faba bean	中国新疆 Xinjiang, China
195	H0003027	青海6号 Qinghai No.6	中国青海 Qinghai, China
196	H0003030	蚕豆 Faba bean	中国河北 Hebei, China
197	H0003109	蚕豆 Faba bean	中国河北 Hebei, China
198	H0003112	白马牙蚕豆 Baimaya faba bean	中国内蒙古 Inner Mongolia, China
199	H0003126	马牙蚕豆 Maya faba bean	中国内蒙古Inner Mongolia, China
200	H0003128	泰县青皮 Taixian green husk	中国江苏Jiangsu, China
201	H0003186	蚕豆 Faba bean	中国江苏 Jiangsu China
202	H0003191	昆山青皮 Kunshan green husk	中国江苏Jiangsu, China
203	H0003192	浦口蚕豆 Pukou faba bean	中国江苏Jiangsu, China
204	H0003195	蚕豆 Faba bean	中国湖南 Hunan, China
205	H0003210	蚕豆 Faba bean	中国湖南 Hunan, China
206	H0003305	小胡豆 small faba bean	中国贵州 Guizhou, China
207	H0003333	桐绵胡豆 Tongmian faba bean	中国贵州 Guizhou, China
208	H0003362	大蚕豆 Big faba bean	中国云南 Yunnan China
209	H0003370	甸中大白豆 Dianzhong big white bean	中国云南 Yunnan, China
210	H0003375	小粒豆 Small faba bean	中国云南 Yunnan, China
211	H0003404	漾田蚕豆 Yangtian faba bean	中国云南 Yunnan China
212	H0003430	马料豆 Maliao bean	中国云南 Yunnan, China
213	H0003431	云豆83-324 Yun bean 83-324	中国云南 Yunnan, China
214	H0003433	青海3 Qinghai No.3	中国青海 Qinghai, China
215	H0003761	蚕豆 Faba bean	中国江西 Jiangxi, China
216	H0003897	蚕豆 Faba bean	中国湖南 Hunan, China
217	H0003937	纳足胡豆 Nazu faba bean	中国四川 Sichuan, China
218	H0003950	大金本地种 Daikin native species	中国四川 Sichuan, China
219	H0004015	小胡豆 small faba bean	中国陕西 Shanxi, China
220	H0004033	糠胡豆 Kang faba bean	中国陕西 Shanxi, China
221	H0004113	7029 11	德国 Germany
222	H0004288	白塔青皮 Baita green husk	中国江苏 Jiangsu, China
223	H0004291	钟庄小青皮 Zhongzhuang small green husk	中国江苏 Jiangsu, China
224	H0004297	小黄山青皮 Xiaohuangshan green husk	中国江苏Jiangsu, China
225	H0004304	钟庄白皮 Zhongzhuang white skin	中国江苏Jiangsu, China
226	H0004311	窑湾白皮 Yaowan white skin	中国江苏Jiangsu, China
227	H0004360	南坡小蚕豆 South slope small faba bean	中国广西 Guangxi China
228	H0004363	小狗豆 Puppy beans	中国广西Guangxi, China
229	H0004518	清邑大粒豆 Qingyi Large grain faba bean	中国云南Yunnan, China
230	H0004558	Alfud	法国 France
231	H0004564	引98-22 Yin 98-22	日本 Japan
232	H0004805	金钟豆 Jinzhong bean	中国云南Yunnan, China
233	H0004812	金钟豆 Jinzhong bean	中国云南Yunnan, China
234	H0004957	L1443	法国 France
235	H0005075	蚕豆 faba bean	中国青海 Qinghai, China
236	H0005088	陈桥白皮蚕豆 ChenqiaoBaipi faba bean	中国江苏 Jiangsu, China
237	H0005089	南通三白 NantongSanbai	中国江苏 Jiangsu, China
238	H0005091	通蚕5号 Tongcan No.5	中国江苏 Jiangsu, China
239	H0005094	苏89027 Su 89027	中国江苏 Jiangsu, China
240	H0005277	北塬尕蚕 Beiyuanga faba bean	中国甘肃 Gansu, China
241	H0005345	法国410-1 France 410-1	法国 France
242	H0005349	伊拉克45-1 Iraq 45-1	伊拉克 Iraq
243	H0005351	渭源马牙蚕 WeiyuanMaya faba bean	中国甘肃 Gansu, China
244	H0005353	加拿大60H Canada 60H	加拿大 Canada
245	H0005355	法国410 France 410	法国 France
246	H0005383	康马牙选(红皮) Kangmayaxuan (Redskins)	中国甘肃 Gansu, China
247	H0005388	岷县羊眼蚕豆 MinxianYangyan faba bean	中国甘肃 Gansu, China
248	H0005389	民和尕豆126 Minhega bean 126	中国甘肃 Gansu, China
249	H0005680	蚕豆 Faba bean	中国河北张家口 Zhangjiakou, Hebei, China
250	H0005837	胡豆 Faba bean	中国重庆 Chongqing, China
251	H0005839	胡豆 faba bean	中国重庆 Chongqing, China
252	H0005852	临蚕2号 Lincan No.2	中国甘肃 Gansu, China
253	H0005867	蚕豆 Faba bean	中国重庆 Chongqing, China
254	H0005913	中品CD-09 Zhongpin CD-09	俄罗斯 Russia
255	H0006305	涡阳大青花 Guoyangdaqinghua	中国安徽 Anhui, China
256	H0006306	合肥蚕豆 Hefei faba bean	中国安徽 Anhui, China
257	H0006309	达县福寿本地胡豆 DaxianFushou Local faba bean	中国四川 Sichuan, China
258	H0006312	金川本地胡豆Jinchuan local faba bean	中国四川 Sichuan, China
259	H0006313	峨眉胡豆 Emei faba bean	中国四川 Sichuan, China
260	Q2	青海12 Qinghai No.2	中国青海 Qinghai, China

Table S1

Table S2

Original data of starch content in 260 faba bean materials for two years"

序号 Serial number	全国统一编号 Unified national numbering	总淀粉含量 Total starch content (%)		直链淀粉含量 Amylose content (%)		支链淀粉含量 Amylopectin content (%)
序号 Serial number	全国统一编号 Unified national numbering	2019	2020	2019	2020	2019	2020
1	H0000002	52.35	38.06	12.81	12.05	39.54	26.01
2	H0000006	40.35	26.69	9.36	7.36	30.99	19.33
3	H0000015	35.55	36.06	7.24	10.8	28.31	25.26
4	H0000016	45.2	31.76	10	9.84	35.21	21.91
5	H0000039	46.44	36.15	10.56	10.68	35.88	25.48
6	H0000090	33.87	49.87	8.61	15.42	25.27	34.45
7	H0000091	47.89	42.96	14.29	11.93	33.6	31.03
8	H0000095	34.53	43.67	6.65	13.15	27.88	30.52
9	H0000109	49.31	48.2	12.79	14.77	36.52	33.44
10	H0000110	41.1	40.36	9.05	11.43	32.04	28.92
11	H0000113	46.49	51.78	11.02	14.8	35.47	36.98
12	H0000116	52.44	45.18	13.71	12.17	38.74	33.01
13	H0000117	39.87	39.24	9.3	12.09	30.57	27.15
14	H0000138	48	45.6	11.18	12.59	36.82	33.01
15	H0000152	44	42.81	9	11.23	34.99	31.58
16	Q15	43.19	43.18	9.97	11.28	33.22	31.89
17	H0000158	43.19	43.18	9.97	11.28	33.22	31.89
18	H0000167	43.23	54.32	10.23	16.16	33	38.16
19	H0000172	48.98	44.73	14.16	14.35	34.83	30.38
20	H0000184	42.43	50.43	9.16	12.82	33.26	37.61
21	H0000189	38.94	48.26	7.53	14.82	31.41	33.44
22	H0000190	40.45	44.26	12.27	11.92	28.18	32.34
23	H0000191	33.68	37.8	6.22	10.93	27.46	26.86
24	H0000193	47.69	41.88	11.82	11.28	35.87	30.6
25	H0000195	39.13	42.72	10.89	11.56	28.23	31.17
26	H0000200	49.41	45.23	10.76	12.95	38.66	32.28
27	H0000201	40.33	42.74	9.06	10.74	31.27	32
28	H0000203	37.93	40.47	8.27	11.57	29.66	28.91
29	H0000207	43	40.83	8.87	12.46	34.13	28.37
30	H0000216	43.34	55.12	9.19	15.93	34.15	39.19
31	H0000218	43.15	42.1	8.26	11.81	34.89	30.29
32	H0000242	46.11	50.85	11.41	12.44	34.7	38.4
33	H0000244	34.26	51.72	7.31	10.93	26.95	40.79
34	H0000245	47.44	51.93	11.42	15	36.02	36.93
35	H0000248	38.13	38.92	8.41	11.54	29.71	27.38
36	H0000285	43.19	43.18	9.97	11.28	33.22	31.89
37	H0000308	45.58	45.79	8.15	12.19	37.43	33.6
38	H0000334	43.19	43.18	9.97	11.28	33.22	31.89
39	H0000341	42.38	44.03	8.87	10.79	33.51	33.24
40	H0000360	37.92	54.79	8.37	15.38	29.55	39.41
41	H0000363	45.14	48.23	8.6	11.49	36.54	36.74
42	H0000364	49.23	50.29	15.71	14.11	33.52	36.18
43	H0000365	37.83	57.47	8.25	15.4	29.58	42.07
44	H0000376	35.09	47.29	6.28	11.75	28.81	35.54
45	H0000389	42.07	31.88	10.01	7.46	32.06	24.42
46	H0000423	39.13	46.78	8.55	12.47	30.58	34.31
47	Q13	43.19	43.18	9.97	11.28	33.22	31.89
48	H0000432	33	52.52	7.86	11.85	25.14	40.67
49	H0000435	43.19	43.18	9.97	11.28	33.22	31.89
50	H0000438	48.87	36.34	12.41	9.77	36.47	26.57
51	Q12	36.11	42.03	9.01	10.6	27.1	31.43
52	H0000456	41.23	50.63	9.04	13.17	32.19	37.46
53	H0000460	43.19	43.18	9.97	11.28	33.22	31.89
54	H0000492	36.45	53.49	8.18	13.73	28.27	39.76
55	H0000508	28.18	31.15	8.46	8.73	19.72	22.42
56	H0000509	47.46	40.96	11.23	10.38	36.22	30.58
57	H0000513	39.32	42.37	7.82	11.87	31.5	30.49
58	H0000518	33.49	48.29	5.56	11.72	27.93	36.57
59	H0000539	42.64	39.25	9	10.45	33.65	28.8
60	H0000541	45.42	42.88	10.78	11.12	34.64	31.76
61	H0000549	44.21	43.58	10	9.57	34.2	34.01
62	H0000552	46.2	44.71	9.61	11.28	36.59	33.42
63	H0000570	39.64	46.8	9.04	11.23	30.59	35.57
64	H0000577	36.99	36.08	9.54	10.65	27.46	25.43
65	H0000578	41.73	37.3	8.25	9.06	33.48	28.25
66	H0000605	47.01	37.72	15.55	10.71	31.46	27.01
67	H0000607	48.33	51.23	10.91	14.59	37.43	36.64
68	H0000693	49.41	53.89	10.79	14.97	38.62	38.92
69	H0000698	45.45	39.19	10.74	10.94	34.71	28.25
70	H0000701	47.87	50.34	11.62	12.37	36.25	37.97
71	H0000782	44.12	54.27	10.79	16.1	33.33	38.17
72	H0000802	45.74	48.25	11.17	14.29	34.58	33.96
73	H0000805	54.27	45.55	14.08	13.65	40.19	31.9
74	H0000807	43.62	44.15	7.92	11.24	35.71	32.91
75	H0000809	43.83	49.31	9.09	13.23	34.74	36.08
76	H0000811	41.02	39.05	8.16	10.36	32.85	28.69
77	H0000813	35.68	43.97	6.1	12.98	29.58	30.99
78	H0000815	37.89	46.54	8.8	14.08	29.08	32.46
79	H0000825	49.62	51.14	10.73	12.33	38.89	38.81
80	H0000876	49.92	40.4	7.4	10.22	42.52	30.19
81	H0000918	33.54	52.07	8.42	10.89	25.12	41.19
82	H0000930	34.87	39.88	8.36	8.6	26.51	31.28
83	H0001019	38.15	49.67	14.4	11.17	23.75	38.5
84	H0001033	37.88	45.2	8.3	10.4	29.58	34.8
85	H0001034	42.62	42.8	8.47	9.32	34.15	33.48
86	H0001035	42.64	41.8	9.51	8.13	33.13	33.67
87	H0001037	29.71	40.24	6.81	13.17	22.9	27.07
88	H0001039	43.19	43.18	9.97	11.28	33.22	31.89
89	H0001075	45.78	45.04	11.11	9.22	34.67	35.82
90	H0001080	45.94	44.89	9.99	8.74	35.94	36.15
91	H0001082	36.07	45.09	7.19	8.92	28.88	36.18
92	H0001085	46.22	40.66	11.03	9.42	35.19	31.25
93	H0001086	37.88	43.29	8.91	15.16	28.97	28.13
94	H0001091	44.59	40.77	10.57	8.11	34.02	32.66
95	H0001105	33.96	45.66	10.1	9.7	23.86	35.95
96	H0001108	38.5	45.77	11.95	8.63	26.54	37.14
97	H0001109	37.11	47.88	9.6	10.42	27.51	37.46
98	H0001112	37.01	51.35	7.08	12.75	29.93	38.6
99	H0001114	28.2	46.08	6.02	12.38	22.19	33.7
100	H0001290	41.41	41.72	8.33	9.56	33.08	32.16
101	H0001346	49.03	46.11	11.27	15.45	37.76	30.66
102	H0001381	43.19	43.18	9.97	11.28	33.22	31.89
103	H0001392	39.58	38.94	9.31	9.99	30.28	28.94
104	H0001402	45.16	40.71	10.2	9.27	34.96	31.44
105	H0001407	56	46.22	12.79	12.43	43.21	33.78
106	H0001431	35.71	43.56	6.59	11.77	29.11	31.79
107	H0001432	39.07	44.88	9.85	11.38	29.22	33.5
108	H0001433	44.81	28.99	12.8	8.35	32.01	20.64
109	H0001438	47.87	48.48	8.97	12.35	38.89	36.13
110	H0001600	34.38	51.36	7.27	12.82	27.11	38.54
111	H0001602	36.84	45.76	10.43	11.06	26.4	34.7
112	H0001624	43.11	48.41	7.61	10.8	35.5	37.61
113	H0001629	45.04	49.33	10.88	9.67	34.17	39.66
114	H0001633	46.04	49.45	9.17	10.81	36.87	38.64
115	H0001635	37.19	47.24	8.05	10.79	29.13	36.45
116	H0001637	38.74	38.76	9.13	7.63	29.61	31.13
117	H0001645	49.88	44.11	11.5	10.05	38.38	34.05
118	H0001652	43.12	44.56	10.2	9.74	32.92	34.83
119	H0001667	52.16	49.2	9.8	10.06	42.37	39.14
120	H0001668	43.46	40.36	7.47	9.69	35.99	30.68
121	H0001669	41.41	41.16	8.65	8.68	32.76	32.48
122	H0001671	38.99	55.3	8.47	12.83	30.52	42.47
123	H0001693	56.34	42.68	13.42	9.52	42.92	33.17
124	H0001706	48.45	49.92	10.19	12.21	38.26	37.71
125	H0001718	42.99	48.33	9.7	14.63	33.29	33.7
126	H0001722	35.79	53.87	5.89	15.75	29.89	38.12
127	H0001724	50.34	51.67	9.93	12.36	40.41	39.31
128	H0001731	50.92	48.65	14.13	11.96	36.79	36.69
129	H0001737	46.39	44.77	12.99	11.81	33.4	32.96
130	H0001758	48.22	47.56	11.45	13.28	36.77	34.28
131	H0001760	43.91	34.39	8.28	10.15	35.63	24.23
132	H0001761	43.66	34.92	10.73	9.18	32.93	25.75
133	H0001773	48.71	21.14	10.01	6.13	38.7	15.01
134	H0001775	47.86	38.08	8.57	10.34	39.3	27.74
135	H0001776	35.61	33.96	8.37	9.75	27.25	24.21
136	H0001777	45.24	29.99	10.25	8.19	34.98	21.8
137	H0001780	48.12	29.1	10.73	7.85	37.39	21.25
138	H0001781	47.82	33.02	12.46	7.67	35.35	25.34
139	H0001784	50.69	31.78	13.06	9	37.63	22.78
140	H0001791	44.56	34.11	9.51	9.22	35.05	24.89
141	H0001796	19.21	35.35	4.15	8.83	15.06	26.53
142	H0001797	36.14	36.34	9.07	8.78	27.07	27.57
143	H0001802	40.19	39.73	8.45	10.17	31.74	29.56
144	H0001805	38.93	32.07	6.77	8.1	32.16	23.98
145	H0001806	48.76	34.76	11.13	9.4	37.63	25.37
146	H0001808	38.89	31.06	7.86	7.71	31.03	23.36
147	H0001854	44.5	35.57	8.77	9.23	35.73	26.34
148	H0001855	49.45	35.61	15.07	8.44	34.38	27.17
149	H0001859	38.61	45.69	7.45	10.58	31.16	35.11
150	H0001863	50.61	38.5	9	9.15	41.61	29.35
151	H0001864	37.33	50.25	9.8	12.01	27.53	38.24
152	H0001865	34.1	44.24	7.39	12.53	26.71	31.71
153	H0001877	42.62	33.97	10.95	8.41	31.67	25.56
154	H0001881	36.46	47.88	8.01	15.26	28.46	32.62
155	H0001884	42.31	33.94	12.45	10.19	29.86	23.74
156	H0001897	50.35	42.78	11.74	10.87	38.61	31.91
157	H0001900	45.27	40.2	10.35	12.79	34.92	27.41
158	H0001935	52.77	36.7	12.44	9.08	40.33	27.61
159	H0001939	37.21	34.82	9.55	9.92	27.67	24.9
160	H0001940	50.09	44.2	11.39	12.91	38.7	31.29
161	H0001947	46.11	37.91	10.48	11.23	35.63	26.68
162	H0001953	45.14	44.66	11.55	14.25	33.59	30.41
163	H0001955	40.47	52.37	13.28	13.46	27.19	38.91
164	H0002297	33.9	38.85	8.55	7.69	25.35	31.16
165	H0002308	51.26	41.91	11.5	10.61	39.77	31.3
166	H0002311	45.46	50.89	10.04	12.23	35.43	38.66
167	H0002314	42.85	48.67	11.98	11.75	30.87	36.92
168	H0002332	43.79	47.56	10.36	12.76	33.43	34.81
169	H0002368	45.24	50.98	10.03	17.18	35.2	33.8
170	H0002375	42.73	42.15	10.1	13.23	32.63	28.92
171	H0002376	50.05	42.5	11.89	10.3	38.16	32.2
172	H0002382	42.12	52.7	10.8	12.61	31.32	40.09
173	H0002409	44.19	46.5	9.43	11.19	34.75	35.32
174	H0002414	47.78	50.17	14.87	12.97	32.9	37.21
175	H0002431	41.81	38.05	7.51	9.37	34.3	28.68
176	H0002432	47.87	46.66	11.5	10.92	36.37	35.74
177	H0002437	38.88	49.12	9.05	13.43	29.83	35.69
178	H0002469	44.12	49.86	10.58	13.66	33.54	36.2
179	H0002470	46.89	49.32	12.3	13.51	34.58	35.81
180	H0002473	47.42	36.94	10.32	9.99	37.1	26.95
181	H0002474	42.93	43.64	7.91	12.65	35.02	30.99
182	H0002475	55.44	42.14	11.74	10.79	43.7	31.35
183	H0002492	40.36	41.88	10.48	12.05	29.88	29.83
184	H0002503	49.63	45.04	11.12	13.42	38.51	31.62
185	H0002505	50.47	39.53	10.03	10.86	40.44	28.66
186	H0002515	41.76	39.53	9.85	10.61	31.91	28.92
187	H0002516	44.6	40.99	12.42	12.28	32.18	28.7
188	H0002517	45.7	48.06	9.48	12.08	36.22	35.98
189	H0002527	38.92	37.05	9.84	9.5	29.08	27.55
190	H0002531	37.61	38.99	8.62	10.75	28.99	28.23
191	H0002542	37.05	38.36	7	11.75	30.06	26.61
192	H0002621	55.06	45.9	12.96	13.92	42.1	31.98
193	H0002622	42.11	41.07	12.9	11.19	29.21	29.88
194	H0002693	45.39	42.93	14.55	10.18	30.84	32.75
195	H0003027	39.51	40.9	7.99	11.35	31.52	29.54
196	H0003030	45.93	50.03	9.59	13.2	36.34	36.83
197	H0003109	42	47.96	8.8	14.15	33.2	33.81
198	H0003112	43.24	42.27	9.27	13.52	33.97	28.75
199	H0003126	44.53	50.29	8.84	14.62	35.69	35.67
200	H0003128	49.52	42.6	11.85	11.49	37.67	31.12
201	H0003186	43.48	39.56	9.06	9.83	34.41	29.74
202	H0003191	48.82	40.74	10.99	9.79	37.83	30.94
203	H0003192	47.56	43.62	10.54	11.22	37.02	32.4
204	H0003195	48.41	46.19	12.86	10.85	35.55	35.34
205	H0003210	35.32	31.89	8.71	6.98	26.61	24.91
206	H0003305	41.79	43.42	8.83	11.57	32.96	31.85
207	H0003333	39.88	35.48	8.78	7.84	31.11	27.64
208	H0003362	44.62	47.6	9.59	13.45	35.03	34.15
209	H0003370	40.14	50.02	5.98	14.45	34.16	35.57
210	H0003375	39.41	37.44	10	10.79	29.41	26.66
211	H0003404	45.12	42.54	10.64	14.66	34.47	27.88
212	H0003430	41.51	37.92	10.38	8.13	31.12	29.79
213	H0003431	38.22	43.37	8.34	9.57	29.87	33.8
214	H0003433	52.55	44.47	13.12	10.56	39.44	33.9
215	H0003761	46.4	31.72	11.37	7.07	35.03	24.65
216	H0003897	39.97	47.81	10.76	11.38	29.21	36.43
217	H0003937	43.5	46.28	10.11	10.76	33.38	35.52
218	H0003950	43.19	43.18	9.97	11.28	33.22	31.89
219	H0004015	43.19	43.18	9.97	11.28	33.22	31.89
220	H0004033	48.05	48.07	12.54	8.1	35.52	39.96
221	H0004113	34.54	47.46	8.14	12.41	26.39	35.04
222	H0004288	40.55	37.49	9.52	10.03	31.03	27.46
223	H0004291	47.14	40.7	9.25	10.83	37.89	29.87
224	H0004297	47.12	41.34	11.4	11.1	35.71	30.24
225	H0004304	33.8	39.4	7.71	9.15	26.09	30.25
226	H0004311	37.49	37.3	9.1	8.92	28.39	28.38
227	H0004360	42.38	43.88	9.36	11.26	33.01	32.62
228	H0004363	34.94	44.47	9.57	11.44	25.37	33.04
229	H0004518	47.78	46.95	11.07	12.15	36.71	34.8
230	H0004558	41.96	46.01	9.78	11.71	32.18	34.3
231	H0004564	46.7	48.48	9.76	12.3	36.94	36.18
232	H0004805	40.7	37.25	8.5	8.73	32.19	28.52
233	H0004812	40.2	43.39	8.07	11.44	32.13	31.95
234	H0004957	46.32	37.29	9.55	8.66	36.77	28.63
235	H0005075	49.77	34.63	10.37	8.65	39.41	25.98
236	H0005088	45.16	40.71	12.29	10.68	32.88	30.03
237	H0005089	46.77	44.92	11.92	12.22	34.85	32.7
238	H0005091	42.73	41.27	10.76	9.61	31.97	31.67
239	H0005094	43.4	34.64	11.37	7.84	32.03	26.8
240	H0005277	50.48	52.54	13.9	14.12	36.58	38.42
241	H0005345	37.18	50.62	7.58	12.82	29.6	37.8
242	H0005349	45.15	43.76	9.87	12.68	35.28	31.08
243	H0005351	54.69	49.87	12.2	14.26	42.48	35.6
244	H0005353	53.52	37.65	14.41	9.15	39.11	28.5
245	H0005355	47.93	43.34	11.27	11.25	36.65	32.09
246	H0005383	50.23	46.46	11.71	11.06	38.52	35.41
247	H0005388	51.08	40.12	13.22	10.53	37.86	29.59
248	H0005389	46.64	44.74	11.51	11.94	35.13	32.8
249	H0005680	52.12	48.74	12.04	11.26	40.08	37.48
250	H0005837	41.41	41.76	9.67	10.55	31.73	31.21
251	H0005839	45.39	40.83	12.97	11.67	32.42	29.16
252	H0005852	41.08	35.43	8.27	10.46	32.81	24.97
253	H0005867	46.51	44.41	9.4	13	37.1	31.41
254	H0005913	47.07	48.06	9.21	12.98	37.87	35.08
255	H0006305	37.31	38.53	7.49	10.01	29.82	28.52
256	H0006306	39.67	24.7	7.21	6.76	32.47	17.94
257	H0006309	37.18	37.11	8.01	9.18	29.17	27.93
258	H0006312	35.93	38.28	6.74	10.12	29.19	28.16
259	H0006313	52.85	51.13	13.75	14.31	39.1	36.82
260	Q2	43.19	43.18	9.97	11.28	33.22	31.89

Table S2

Table 1

Table 2

Diversity statistics of 132 SSR markers"

SSR标记 SSR marker	等位变异数 Allele number	PIC值 PIC value	SSR标记 SSR marker	等位变异数 Allele number	PIC值 PIC value
SSR-14430	5	0.546004366	SSR-10832	4	0.367983408
T135	8	0.638809510	SSR-12586	7	0.688982215
CAASES-V2109	5	0.398398789	SSR-12332	5	0.536771755
CAASES-V2188	6	0.481120827	SSR-17507	6	0.672629597
CAASES-V2220	6	0.655452925	SSR-11885	6	0.468655886
CAASES-V2275	3	0.370899893	SSR-11448	5	0.674331257
CAASES-V2276	3	0.462231328	SSR-17506	4	0.434827275
CAASES-V2285	6	0.548163715	CAASES-V2888	3	0.174961802
CAASES-V3052	6	0.608552279	CAASES-V2900	7	0.696296052
Y67	6	0.544591908	SSR-11365	5	0.416189821
SSR标记 SSR marker	等位变异数 Allele number	PIC值 PIC value	SSR标记 SSR marker	等位变异数 Allele number	PIC值 PIC value
Y240	5	0.646349940	SSR-11862	3	0.368540222
SSR-1914	2	0.366634918	SSR-10927	3	0.477998444
SSR-3581	7	0.393776875	SSR-17488	4	0.386326852
CAASES-V3027	3	0.353390469	SSR-12192	10	0.777168952
CAASES-V2970	3	0.339240345	SSR-12180	4	0.423140888
CAASES-V2268	3	0.555958839	SSR-11621	5	0.606260559
CAASES-V2138	5	0.505818789	EST-868	5	0.645056220
CAASES-V2253	3	0.357520958	SSR-12695	5	0.370319539
CAASES-V2986	7	0.582134815	CAASES-V2460	6	0.614920246
SSR-14765	6	0.686044392	SSR-12066	6	0.479818890
CAASES-V2873	6	0.626384886	CSSR-3165	4	0.436915836
CAASES-V2882	6	0.657917578	EST-657	4	0.418280290
CAASES-V3035	4	0.616186156	SSR-10757	3	0.428386499
SSR-14892	3	0.395098689	CAASES-V1761	4	0.408152627
CAASES-V3017	7	0.686841952	SSR-11342	7	0.597122677
SSR-13508	3	0.388112318	CAASES-V1929	6	0.477722129
CAASES-V3056	3	0.415987468	CAASES-V2542	6	0.481698866
CAASES-V3043	7	0.615888838	CAASES-V2574	2	0.322170691
CAASES-V2979	3	0.206205925	CAASES-V2073	4	0.617839636
CAASES-V2687	5	0.318851968	CAASES-V2493	5	0.491490441
CAASES-V2704	4	0.334290229	CAASES-V1846	2	0.350108424
CAASES-V2745	6	0.520118098	CAASES-V1702	3	0.377880223
CAASES-V2772	4	0.486101228	CAASES-V1693	4	0.562444226
CAASES-V2716	3	0.294313640	CRRS-3883	6	0.533506078
T179	7	0.619039383	SSR-16592	5	0.656288183
EST-475	2	0.208612127	CAASES-V2949	2	0.149403691
SSR-14109	7	0.591871872	CAASES-V2958	5	0.512034193
CAASES-V3012	8	0.559919609	FBFS0809	3	0.421741390
T19	11	0.659173983	SSR-18329	3	0.438471591
CAASES-V2991	5	0.652378908	CAASES-V1778	3	0.330519810
CAASES-V2277	6	0.266579674	CAASES-V1797	3	0.038506792
CAASES-V734	4	0.608583511	CAASES-V2653	5	0.509415514
CAASES-V3053	6	0.744375932	FBES0135	4	0.582209878
CAASES-V3051	4	0.593266116	SSR-12842	6	0.494012490
CAASES-V3058	10	0.644674708	SSR-13593	5	0.438436602
CAASES-V3031	9	0.737669010	CAASES-V1133	4	0.482122844
CAASES-V2720	5	0.351270737	CAASES-V2729	4	0.524157331
SSR-17892	4	0.339610677	SSR-14219	4	0.625374004
CAASES-V2731	4	0.480439927	SSR-12526	2	0.349950050
CAASES-V2733	3	0.484891353	SSR-13380	4	0.594564813
CAASES-V2698	9	0.644541260	SSR-12667	6	0.437876859
CAASES-V2937	4	0.333728761	CAASES-V2646	8	0.718458284
CAASES-V2930	3	0.459626286	CAASES-V1820	7	0.662853014
SSR-17611	4	0.443153884	CAASES-V1446	3	0.383681231
SSR-13844	3	0.387814774	EST-24	5	0.467347043
SSR标记 SSR marker	等位变异数 Allele number	PIC值 PIC value	SSR标记 SSR marker	等位变异数 Allele number	PIC值 PIC value
SSR-11854	3	0.549082724	CAASES-V1916	2	0.348207710
SSR-13584	5	0.557078842	CAASES-V1836	5	0.624983881
SSR-14447	6	0.745824355	SSR-16885	4	0.516754205
SSR-11723	5	0.674373900	CAASES-V2228	4	0.284764706
SSR-13067	5	0.499203703	FBES0142	5	0.565272774
SSR-12751	4	0.654854111	CAASES-V2388	5	0.611937849
SSR-16047	5	0.412237622	CAASES-V2280	6	0.741593509
SSR-11967	2	0.219037825	CAASES-V2015	9	0.711608618
SSR-12911	4	0.324329424	SSR-10894	3	0.560535944
SSR-15868	3	0.337260650	SSR-18215	3	0.288429668
SSR-12503	4	0.388123048	CAASES-V2103	3	0.511196932

Table 2

Fig. 1

Fig. 2

Fig. 3

Fig. 4

Table 3

Association analysis between SSR markers and agronomic traits"

表型 Trait	标记 Marker	GLM		标记 Marker	MLM
表型 Trait	标记 Marker	2019	2020	标记 Marker	2019	2020
总淀粉含量	CAASES-V3017	0.0904		CAASES-V3017	0.0902
Total starch content (%)	CAASES-V1836	0.0701		CAASES-V1836	0.0664
	CAASES-V2900	0.0655		CAASES-V2900	0.0646
	SSR-10927	0.0588		SSR-10927	0.0541	0.0366
	SSR-1914		0.0363	SSR-1914		0.0269
	CAASES-V2460		0.0691	CAASES-V2460		0.0560
	CAASES-V1133		0.0524	CAASES-V1133		0.0404
表型 Trait	标记 Marker	GLM		标记 Marker	MLM
表型 Trait	标记 Marker	2019	2020	标记 Marker	2019	2020
	CAASES-V2228		0.0454	CAASES-V2228		0.0334
	SSR-14447		0.0765	SSR-14447		0.0636
	SSR-12667		0.0627	SSR-12667		0.0435
	SSR-12842		0.0561	SSR-12842		0.0417
	CAASES-V2888		0.0387	CAASES-V2888		0.0327
	CAASES-V2277		0.0492	CAASES-V2188		0.0561
	SSR-13844		0.0255
直链淀粉含量	CAASES-V2015	0.1146		CAASES-V2015	0.1142
Amylose content (%)	SSR-11342		0.0828	SSR-11342		0.0525
	SSR-12667		0.0641	SSR-12667		0.0333
	CAASES-V2228		0.0382	CAASES-V2228		0.0246
	CAASES-V3035		0.0431	CAASES-V3035		0.0337
	SSR-13380		0.0661	SSR-13380		0.0412
	CSSR-3165		0.0461	CAASES-V2277	0.0440
	SSR-13593	0.0806
	CAASES-V2188		0.0471
	SSR-14447		0.0614
支链淀粉含量	CAASES-V3017	0.1045		CAASES-V3017	0.1039
Amylopectin content (%)	CAASES-V1836	0.0776		CAASES-V1836	0.0715
	CAASES-V2900	0.0655		CAASES-V2900	0.0609
	FBFS0809	0.0308		FBFS0809	0.0277
	CAASES-V3056	0.0682		CAASES-V3056	0.0410
	CAASES-V1693	0.0351		CAASES-V1693	0.0310
	SSR-10927	0.0493	0.0502	SSR-10927	0.0424	0.0450
	CAASES-V2460		0.0862	CAASES-V2460		0.0731
	CAASES-V1133		0.0700	CAASES-V1133		0.0560
	SSR-12842		0.0793	SSR-12842		0.0659
	SSR-1914		0.0406	SSR-1914		0.0322
	CAASES-V2188		0.0719	CAASES-V2188		0.0600
	CAASES-V2228		0.0386	CAASES-V2228		0.0328
	SSR-14447		0.0703	SSR-14447		0.0651
	SSR-12667		0.0529	SSR-12667		0.0433
	CAASES-V2888		0.0391	CAASES-V2888		0.0358

Table 3

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年份 Year	性状 Trait	均值±标准差 Mean±SD (%)	变异范围 Range of variation (%)	偏度 Skewness	峰度 Kurtosis	变异系数 CV (%)
2019	总淀粉含量 Total starch content	43.19±5.71	19.21-56.34	-0.351	0.522	13.21
	直链淀粉含量 Amylose content	9.97±2.05	4.15-15.71	0.317	0.001	20.52
	支链淀粉含量 Amylopectin content	33.22±4.48	15.06-43.70	-0.365	0.583	13.47
2020	总淀粉含量 Total starch content	43.18±6.19	21.14-57.47	-0.406	0.150	14.33
	直链淀粉含量 Amylose content	11.28±2.08	6.13-17.18	0.188	-0.285	18.47
	支链淀粉含量 Amylopectin content	31.89±4.81	15.01-42.47	-0.342	0.016	15.09

Genetic diversity and its association analysis of SSR markers with starch content in faba bean (Vicia faba L.)

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