转录组与代谢组联合分析揭示遮阴胁迫下甘薯的代谢响应途径

doi:10.3724/SP.J.1006.2023.24137

Abstract

Abstract:

Sweetpotato is a heliophile crop. However, it is usually shaded in the lower position in the interplanting cultivation mode. During the middle and late field growing period, it often faces rainy weather with little illumination, which affects the dry matter accumulation in tuberous roots. Thus, analyzing the metabolic response pathways of sweetpotato under shade stress will provide the theoretical basis for the varieties’ genetic improvement of shade tolerance. In this study, the sweetpotato variety Zhenghong 23 was exposed to shade stress with 50% light transmittance for 15 days. Results showed that chlorophyll b and the total chlorophyll contents of Zhenghong 23 under shade stress were significantly increased compared with those under natural light. The maximum photochemical efficiency (F_v/F_m), the potential activity (F_v/F_o), and the comprehensive index of photosynthetic performance (PI_ABS) of the chlorophyll photosystem PSII decreased significantly under shade stress. The net photosynthetic rate and water use efficiency decreased significantly, while SOD and POD enzyme activities increased significantly. In addition, shade stress increased significantly the vine length and specific leaf area of Zhenghong 23, but reduced significantly the fresh weight of roots. Transcriptome and metabolome analysis of leaf tissues under shade stress and natural light conditions showed that the DEGs and DMs were mainly enriched in phenylpropanoid biosynthesis, sugar metabolism, sphinolipid metabolism, and arginine biosynthesis pathways. Most of the up-regulated DEGs enriched in the phenylpropanoid biosynthesis pathway were POD enzyme family genes, indicating that the shade stress triggered the ROS scavenging system in sweetpotato. Meanwhile, shade stress reduced sugar metabolism level of sweetpotato, decreased the soluble sugar content of leaves, inhibited both the synthesis and degradation of starch, and blocked the expansion of tuberous roots. In addition, the sphinolipid and arginine metabolism pathways may better adapt sweetptoato plants to shade stress through improving the stability of biomembranes and increase the synthetic substrates of polyamine anti-stress factors. These results provide new theoretical basis for understanding the metabolic response pathways of sweetpotato under shade stress.

Key words: sweetpotato, shade stress, transciptome, metabolome, the response pathways

WANG Yan-Nan, CHEN Jin-Jin, BIAN Qian-Qian, HU Lin-Lin, ZHANG Li, YIN Yu-Meng, QIAO Shou-Chen, CAO Guo-Zheng, KANG Zhi-He, ZHAO Guo-Rui, YANG Guo-Hong, YANG Yu-Feng. Integrated analysis of transcriptome and metabolome reveals the metabolic response pathways of sweetpotato under shade stress[J].Acta Agronomica Sinica, 2023, 49(7): 1785-1798.

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光处理 Light treatment	蔓长 Vine length (cm)	茎节数 Stem internode number	比叶面积 Specific leaf area (cm² g^-1)	根鲜重 Root fresh weight (g)	SOD活性 SOD activity (U g^-1 FW)	POD活性 POD activity (U g^-1 FW)
光照Light	43.5 (6.9)	13.3 (0.5)	211.9 (28.3)	17.9 (9.9)	339.7 (80.2)	1096.1 (230.0)
遮阴Shade	65.7 (9.3)^**	14.8 (2.1)	457.6 (39.2)^***	1.2 (0.5)^*	547.2 (72.8)^**	2366.9 (398.0)^**

光处理 Light treatment	叶绿素a Chlorophyll a	叶绿素b Chlorophyll b	叶绿素a/b Chlorophyll a/b	总叶绿素 Total chlorophyll	F_v/F_m	F_v/F_o	PI_ABS
光照Light	1.08 (0.11)	0.45 (0.12)	2.44 (0.34)	1.53 (0.23)	0.749 (0.022)	2.838 (0.210)	0.570 (0.055)
遮阴Shade	1.21 (0.04)	0.73 (0.05)^**	1.66 (0.15)^**	1.94 (0.05)^*	0.670 (0.033)^**	2.107 (0.364)^*	0.398 (0.046)^**

光处理 Light treatment	净光合速率 Net photosynthetic rate (μmol m^-2 s^-1)	蒸腾速率 Transpiration rate (mmol m^-2 s^-1)	胞间CO₂浓度 Intercellular CO₂ concentration (μmol mol^-1)	气孔导度 Stomatal conductance (mmol m^-2 s^-1)	水汽压亏缺 Vapor pressure deficit (mb)	水分利用效率 Water use efficiency (%)
光照Light	9.13 (0.87)	2.29 (0.53)	241.8 (28.2)	103.5 (8.9)	2.07 (0.18)	4.07 (0.54)
遮阴Shade	2.45 (0.54)^***	1.93 (0.46)	341.0 (13.7)^**	97.3 (10.1)	2.18 (0.23)	1.02 (0.37)^***

样品 Sample name	原始序列数 Raw reads	过滤后序列及占比 Clean reads and percentage (%)	比对到基因组上的reads数及占比 Number and proportion of reads on the genome (%)	单一位置reads数及百分比 Unique mapped reads and percentage (%)	Q30比例 Q30 ratio (%)
S1	46,514,384	45,395,970 (97.60)	32,813,096 (72.28%)	31,148,789 (68.62%)	92.72
S2	45,595,318	44,524,818 (97.65)	32,073,379 (72.03%)	30,310,383 (68.08%)	93.15
S3	48,243,946	46,619,848 (96.63)	33,903,203 (72.72%)	32,580,570 (69.89%)	93.16
S4	45,242,868	43,886,694 (97.00)	31,090,577 (70.84%)	29,766,110 (67.82%)	92.72
L1	51,826,126	50,453,206 (97.35)	36,601,089 (72.54%)	35,349,491 (70.06%)	92.79
L2	52,046,026	50,516,688 (97.06)	35,620,398 (70.51%)	34,152,030 (67.61%)	93.27
L3	43,082,564	42,246,862 (98.06)	29,421,358 (69.64%)	28,373,138 (67.16%)	92.76
L4	45,214,902	43,699,856 (96.65)	30,949,611 (70.82%)	29,612,170 (67.76%)	93.30

Integrated analysis of transcriptome and metabolome reveals the metabolic response pathways of sweetpotato under shade stress

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[2]	CHEN Yi-Hang, TANG Chao-Chen, ZHANG Xiong-Jian, YAO Zhu-Fang, JIANG Bing-Zhi, WANG Zhang-Ying. Construction of core collection of sweetpotato based on phenotypic traits and SSR markers [J]. Acta Agronomica Sinica, 2023, 49(5): 1249-1261.
[3]	WU Shi-Yu, CHEN Kuang-Ji, LYU Zun-Fu, XU Xi-Ming, PANG Lin-Jiang, LU Guo-Quan. Effects of nitrogen fertilizer application rate on starch contents and properties during storage root expansion in sweetpotato [J]. Acta Agronomica Sinica, 2023, 49(4): 1090-1101.
[4]	LIU Ming, FAN Wen-Jing, ZHAO Peng, JIN Rong, ZHANG Qiang-Qiang, ZHU Xiao-Ya, WANG Jing, LI Qiang. Genotypes screening and comprehensive evaluation of sweetpotato tolerant to low potassium stress at seedling stage [J]. Acta Agronomica Sinica, 2023, 49(4): 926-937.
[5]	YAO Zhu-Fang, ZHANG Xiong-Jian, YANG Yi-Ling, HUANG Li-Fei, CHEN Xin-Liang, YAO Xiao-Jian, LUO Zhong-Xia, CHEN Jing-Yi, WANG Zhang-Ying, FANG Bo-Ping. Genetic diversity of phenotypic traits in 177 sweetpotato landrace [J]. Acta Agronomica Sinica, 2022, 48(9): 2228-2241.
[6]	CHEN Lu, ZHOU Shu-Qian, LI Yong-Xin, CHEN Gang, LU Guo-Quan, YANG Hu-Qing. Identification and expression analysis of uncoupling protein gene family in sweetpotato [J]. Acta Agronomica Sinica, 2022, 48(7): 1683-1696.
[7]	JIN Rong, JIANG Wei, LIU Ming, ZHAO Peng, ZHANG Qiang-Qiang, LI Tie-Xin, WANG Dan-Feng, FAN Wen-Jing, ZHANG Ai-Jun, TANG Zhong-Hou. Genome-wide characterization and expression analysis of Dof family genes in sweetpotato [J]. Acta Agronomica Sinica, 2022, 48(3): 608-623.
[8]	ZHANG Hai-Yan, XIE Bei-Tao, JIANG Chang-Song, FENG Xiang-Yang, ZHANG Qiao, DONG Shun-Xu, WANG Bao-Qing, ZHANG Li-Ming, QIN Zhen, DUAN Wen-Xue. Screening of leaf physiological characteristics and drought-tolerant indexes of sweetpotato cultivars with drought resistance [J]. Acta Agronomica Sinica, 2022, 48(2): 518-528.
[9]	CAO Liang, DU Xin, YU Gao-Bo, JIN Xi-Jun, ZHANG Ming-Cong, REN Chun-Yuan, WANG Meng-Xue, ZHANG Yu-Xian. Regulation of carbon and nitrogen metabolism in leaf of soybean cultivar Suinong 26 at seed-filling stage under drought stress by exogenous melatonin [J]. Acta Agronomica Sinica, 2021, 47(9): 1779-1790.
[10]	ZHANG Si-Meng, NI Wen-Rong, LYU Zun-Fu, LIN Yan, LIN Li-Zhuo, ZHONG Zi-Yu, CUI Peng, LU Guo-Quan. Identification and index screening of soft rot resistance at harvest stage in sweetpotato [J]. Acta Agronomica Sinica, 2021, 47(8): 1450-1459.
[11]	NIU Li, BAI Wen-Bo, LI Xia, DUAN Feng-Ying, HOU Peng, ZHAO Ru-Lang, WANG Yong-Hong, ZHAO Ming, LI Shao-Kun, SONG Ji-Qing, ZHOU Wen-Bin. Effects of plastic film mulching on leaf metabolic profiles of maize in the Loess Plateau with two planting densities [J]. Acta Agronomica Sinica, 2021, 47(8): 1551-1562.
[12]	MA Meng, YAN Hui, GAO Run-Fei, KOU Meng, TANG Wei, WANG Xin, ZHANG Yun-Gang, LI Qiang. Construction linkage maps and identification of quantitative trait loci associated with important agronomic traits in purple-fleshed sweetpotato [J]. Acta Agronomica Sinica, 2021, 47(11): 2147-2162.
[13]	Shan-Bin CHEN, Si-Fan SUN, Nan NIE, Bing DU, Shao-Zhen HE, Qing-Chang LIU, Hong ZHAI. Cloning of IbCAF1 and identification on tolerance to salt and drought stress in sweetpotato [J]. Acta Agronomica Sinica, 2020, 46(12): 1862-1869.
[14]	Hai-Yan ZHANG,Bei-Tao XIE,Bao-Qing WANG,Shun-Xu DONG,Wen-Xue DUAN,Li-Ming ZHANG. Evaluation of drought tolerance and screening for drought-tolerant indicators in sweetpotato cultivars [J]. Acta Agronomica Sinica, 2019, 45(3): 419-430.
[15]	ZHANG Hai-Yan,DUAN Wen-Xue,XIE Bei-Tao,DONG Shun-Xu,WANG Bao-Qing,SHI Chun-Yu,ZHANG Li-Ming. Effects of Drought Stress at Different Growth Stages on Endogenous Hormones and Its Relationship with Storage Root Yield in Sweetpotato [J]. Acta Agron Sin, 2018, 44(01): 126-136.