欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2008, Vol. 34 ›› Issue (12): 2184-2189.doi: 10.3724/SP.J.1006.2008.02184

• 耕作栽培·生理生化 • 上一篇    下一篇

氮素穗肥对水稻幼穗细胞分裂素和生长素浓度的影响及其与颖花发育的关系

王夏雯;王绍华;李刚华;王强盛;刘正辉;余翔;丁艳锋*   

  1. 南京农业大学农业部作物生理生态重点开放实验室 / 江苏省信息农业高技术研究重点实验室，江苏南京210095
  • 收稿日期:2008-03-10 修回日期:2008-04-01 出版日期:2008-12-12 网络出版日期:2008-09-06
  • 通讯作者: 丁艳锋
  • 作者简介:王夏雯(1982-),女,甘肃平凉市人,在读硕士,主要从事水稻生理研究
  • 基金资助:

    国家自然科学基金项目(30471016);国家科技支撑计划项目(2006BAD02A03)

Effect of Panicle Nitrogen Fertilizer on Concentrations of Cytokinin and Auxin in Young Panicles of Japonica Rice and Its Relation with Spikelet Development

WANG Xia-Wen,WANG Shao-Hua,LI Gang-Hua,WANG Qiang-sheng,LIU Zheng-Hui,YU Xiang,DING Yan-Feng*   

  1. Key Laboratory of Crop Growth Regulation, Ministry of Agriculture, Nanjing Agricultural University / Hi-Tech Key Laboratory of Information Agriculture of Jiangsu Province, Nanjing 210095, Jiangsu, China
  • Received:2008-03-10 Revised:2008-04-01 Published:2008-12-12 Published online:2008-09-06
  • Contact: DING Yan-Feng

PDF

2567

被引次数

24

摘要：

以粳稻品种武育粳3号和2401为研究材料，在盆栽条件下，设高(HN)、低(LN)两个穗肥施氮水平，对幼穗发育过程中(颖花分化至抽穗后7 d)细胞分裂素(ZRs、iPAs)和生长素(IAA)含量及其动态分别进行测定和比较分析。结果表明，幼穗发育过程中穗中细胞分裂素和生长素含量均表现先降后升的趋势，穗肥的施用(HN处理)提高了各个时期细胞分裂素的含量，尤其是颖花分化期效果最为明显。而生长素的变化情况较为复杂，从颖花分化期到花粉母细胞减数分裂期前LN处理稍高于HN处理，之后HN处理又逐渐超过LN处理。穗发育期间HN处理的ZRs/IAA和iPAs/IAA均高于LN处理，颖花分化期差距最大，随后逐渐减小，到花粉母细胞减数分裂期差异最小，抽穗时比值差距又再次扩大，两个品种表现相似。水稻氮素穗肥的施用具有延长颖花分化时间、增加每穗颖花数的作用，这可能与其提高幼穗分化期尤其是颖花分化期的细胞分裂素含量及其与生长素的比值有关。

关键词: 水稻, 氮素穗肥, 激素, 每穗颖花数

Abstract:

Panicle N fertilizer, which is nitrogen fertilizer applied at panicle development stage, has great influence on the development of rice panicle. Studies have revealed that the beneficial response of nitrogen fertilizer on spikelet development is associated with the increase of spikelets number per panicle. However, little is known concerning the effect of panicle N fertilizer on endogenous hormones in rice young panicle. In the present study, two japonica rice cultivars (Wuyujing 3 and 2401) were used, and two panicle N fertilizer treatments, 120 kg N ha-1 as high nitrogen (HN) and 0 kg N ha-1 as low nitrogen (LN), were conducted to investigate the relationship between spikelets number and hormonal changes during rice panicle differentiation. The results showed that zeatin + zeatin riboside (ZRs), isopen-tenyl adenine (iPAs), and indole-3-acetic acid (IAA) concentrations were the highest at spikelet differentiation stage, sharply declined and remained almost static till heading, and recovered slightly after heading. Panicle N fertilizer treatment resulted in higher concentrations of ZRs and iPAs, especially at the spikelet differentiation stage. Whereas, IAA displayed lower concentration in HN as compared to LN. Moreover, there were higher ratios of ZRs/IAA and iPAs/IAA for HN as compared to LN during the panicle differentiation stage. The difference between them was the maximum at the spikelet differentiation stage and then declined to the minimum before heading, while there was no significant difference between two cultivars. The results suggested that the effect of panicle N fertilizer on spikelet differentiation and numbers per panicle could be associated with the changes of endogenous hormones such as ZRs, iPAs and IAA, either in their concentrations or in their ratios.

Key words: Rice, Panicle nitrogen fertilizer, Hormone, Spikelets per panicle

[1]Sakakibara H, Takei K, Hirose N. Interactions between nitro-gen and cytokinin in the regulation of metabolism and deve- lopment. Trends Plant Sci, 2006, 11: 440-448
[2]Sakakibara H. Cytokinins: activity, biosynthesis, and translocation. Annu Rev Plant Biol, 2006, 57: 431-449
[3]Takei K. Nitrogen-dependent accumulation of cytokinins in root and the translocation to leaf: Implication of cytokinin species that induces gene expression of maize response regu-lator. Plant Cell Physiol, 2001, 42: 85-93
[4]Samuelson M E, Larsson C M. Nitrate regulation of zeatin riboside levels in barley roots: Effects of inhibitors of N assimilation and comparison with ammonium. Plant Sci, 1993, 93: 77-84
[5]Ashikari M, Sakakibara H, Lin S Y, Yamamoto T, Takashi T, Nishimura A. Cytokinin oxidase regulates rice grain produc-tion. Science, 2005, 309: 741-745
[6]Yang J-C(杨建昌), Wang G-Z(王国忠), Wang Z-Q(王志琴), Liu L-J(刘立军), Zhu Q-S(朱庆森). Grain filling characteris-tics and changes of hormonal content in the grains of dry cul-tivated rice during grain filling. Acta Agron Sin (作物学报), 2002, 28(5): 615-621 (in Chinese with English abstract)
[7]Chang E-H(常二华), Wang P(王朋), Tang C(唐成), Liu L-J(刘立军), Wang Z-Q(王志琴), Yang J-C(杨建昌). Con-centrations of cytokinin and abscisic acid in roots and grains and its relationship with grain filling and cooking quality of rice. Acta Agron Sin (作物学报), 2006, 32(4): 540-547 (in Chinese with English abstract)
[8]Zahir Z A, Asghar H N, Arshad M. Cytokinin and its precur-sors for improving growth and yield of rice. Soil Biol Bio-chem, 2001, 33: 405–408
[9]Wei Y-M(魏育明), Zheng Y-L(郑有良). The regulation of multispikelet by endogenous hormones in wheat. J Sichuan Agric Univ (四川农业大学学报), 1998, 16(2): 199–202 (in English with Chinese abstract)
[10]Qiu Z H, Wu J C, Dong B, Li D H, Gu H N. Two-way effect of pesticides on zeatin riboside content in both rice leaves and roots. Crop Prot, 2004, 23: 1131–1136
[11]Kyozuka J. Control of shoot and root meristem function by cytokinin. Curr Opin Plant Biol, 2007, 10: 442–446
[12]Jin D-M(靳德明), Wang W-J(王维金), Lan S-Y(蓝盛银), Xu Z-X(徐珍秀), Yang S-H(杨书化). Dynamic status of en-dogenous IAA, ABA and GA levels in superior and inferior spikelets of heavy panicle hybrid rice during grain filling. J Plant Physiol Mol Biol (植物生理与分子生物学学报), 2002, 28(3): 215–220 (in English with Chinese abstract)
[13]Li Z-T(李宗霆), Zhou X(周燮). Plant Hormones and Immu-noassay Technology (植物激素及其免疫检测技术). Nanjing: Jiangsu Science and Technology Press, 1996. p 179 (in Chi-nese)
[14]Sakakibara H. Nitrate-speci?c and cytokinin-mediated nitro-gen signaling pathways in plants. J Plant Res, 2003, 116: 253–257
[15]Werner T, Motyka V, Laucou V, Smets R. Cytokinin-de?cient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activity. Plant Cell, 2003, 15: 2532–2550
[16]Forde B G. Local and long-range signaling pathways regulat-ing plant responses to nitrate. Annu Rev Plant Biol, 2002, 53: 203–224
[17]Takei K. Multiple routes communicating nitrogen availability from roots to shoots: A signal transduction pathway mediated by cytokinin. J Exp Bot, 2002, 53: 971–977
[18]Miyawaki K, Matsumoto K M, Kakimoto T. Expression of cytokinin biosynthetic isopentenyltransferase genes in Arabi-dopsis: Tissue speci?city and regulation by auxin, cytokinin, and nitrate. Plant J, 2004, 37: 128–138
[19]Corbesier L, Prinsen E, Jacqmard A, Lejeune P, Van Onckelen H. Cytokinin levels in leaves, leaf exudate and shoot apical meristem of Arabidopsis thaliana during ?oral transition. J Exp Bot, 2003, 54: 2511–2517
[20]Hirose N. Functional characterization and expression analysis of a gene, OsENT2, encoding an equilibrative nucleoside transporter in rice suggest a function in cytokinin transport. Plant Physiol, 2005, 138: 196–206
[21]Kyozuka J. Control of shoot and root meristem function by cytokinin. Curr Opin Plant Biol, 2007, 10: 442–446
[1] 金昱何, 王雪菲, 徐张一娃, 缪怡宁, 蒋云杰, 伊莹, 缪德麟, 朱静仪, 钟一帆, 陈铭亨, 方芳, 刘鹏. 外源激素对低温胁迫下大豆叶片叶绿素荧光参数及抗氧化酶系统的影响[J]. 作物学报, 2026, 52(6): 1817-1829.
[2] 胡赵, 钱润, 谢丰璞, 应素平. 水稻SPX基因家族鉴定及响应磷处理的表达分析[J]. 作物学报, 2026, 52(6): 1902-1912.
[3] 邹仪妹, 徐敏, 汪海洋, 姚辉, 王加峰, 刘浩, 任代胜. 两系不育系水稻幼苗根系响应盐胁迫的转录因子调控网络鉴定[J]. 作物学报, 2026, 52(6): 1728-1742.
[4] 闫安, 蒋昆炜, 王蓉圆, 田林, 张璐, 王韵, 徐建龙. 水稻剑叶小维管束数基因SVN7的鉴定与克隆[J]. 作物学报, 2026, 52(5): 1364-1372.
[5] 刘昕萌, 任昊, 张继波, 张吉旺, 赵斌, 任佰朝, 刘鹏, 王洪章. 茉莉酸甲酯(MeJA)缓解高温影响玉米雌穗分化的生理机制[J]. 作物学报, 2026, 52(5): 1561-1572.
[6] 蔡兆琴, 何观咏, 何文, 阮丽霞, 梁振华, 李永珍, 李恒锐, 陈会鲜. 木薯分枝发育过程的动态转录组分析与关键基因发掘[J]. 作物学报, 2026, 52(5): 1430-1441.
[7] 石少阶, 刘凯, 陈姿夷, 王卉颖, 李三和, 周雷, 游艾青. 水稻矮化多分蘖基因DMT1的克隆与功能分析[J]. 作物学报, 2026, 52(4): 1022-1034.
[8] 陈伟, 卫万娟, 赵其兵, 常东伟, 余凌波, 翟鹏飞, 冯志明, 陈宗祥, 任仰涛, 杨鹏, 刘海浪, 李珍富, 杨永乐, 金彦刚, 左示敏. 利用CRISPR/Cas9编辑Hd6基因创制优质早熟水稻新种质[J]. 作物学报, 2026, 52(4): 1046-1056.
[9] 覃奕琰, 付瑶, 苏畅, 李娜, 徐静茹, 程笑然, 张琪, 赵明辉. OsST41调控水稻苗期耐盐性的功能分析[J]. 作物学报, 2026, 52(3): 802-812.
[10] 马亮, 马璐, 张舒钰, 章慧敏, 王仁明, 宋旭东, 张振良, 冒宇翔, 陆虎华, 陈国清, 郝德荣, 周广飞. 玉米苞叶数目转录组分析及候选基因鉴定[J]. 作物学报, 2026, 52(3): 790-801.
[11] 张宇, 刘芳, 蔡诚诚, 杨小华, 吉阿么石扎, 杨元军, 王西瑶. 溴乙烷与赤霉素协同处理破除马铃薯块茎休眠的机理初探[J]. 作物学报, 2026, 52(3): 825-838.
[12] 叶凡, 李帅, 李思宇, 陈云, 窦超银, 刘立军. 不同节水灌溉方式对东北稻区水稻产量和群体质量的影响[J]. 作物学报, 2026, 52(3): 895-907.
[13] 王婵, 吴莹莹, 李文奇, 李霞, 王芳权, 周彤, 杨杰. 基于HRM技术开发水稻抗条纹叶枯病基因STV11功能标记[J]. 作物学报, 2025, 51(9): 2547-2556.
[14] 孔德真, 桑伟, 聂迎彬, 李伟, 徐红军, 李江博, 刘鹏鹏, 田笑明. 小麦AL型细胞质雄性不育系与同型保持系穗花发育时期代谢物变化比较研究[J]. 作物学报, 2025, 51(9): 2454-2466.
[15] 陈惠莹, 何嘉欣, 朱斌, 黄士轩, 周星佑, 伍君权, 杨美艳. 水稻黄单胞菌噬菌体vB_XaS_HDB2的全基因组分析和生物学特性研究[J]. 作物学报, 2025, 51(8): 2087-2099.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2