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作物学报 ›› 2021, Vol. 47 ›› Issue (4): 780-786.doi: 10.3724/SP.J.1006.2021.04122

• 研究简报 • 上一篇    

马铃薯StIgt基因家族的鉴定及其对干旱胁迫的响应分析

秦天元1,2(), 刘玉汇1,2, 孙超1,2, 毕真真1,2, 李安一3, 许德蓉1,2, 王一好1,2, 张俊莲1, 白江平1,2,*()   

  1. 1甘肃省干旱生境作物学重点实验室 / 甘肃省作物遗传改良与栽培种创新重点实验室, 甘肃兰州 730070
    2甘肃农业大学农学院, 甘肃兰州 730070
    3兰州大学草地农业科技学院 / 草地农业生态系统国家重点实验室, 甘肃兰州 730020
  • 收稿日期:2020-06-04 接受日期:2020-10-14 出版日期:2021-04-12 网络出版日期:2020-11-06
  • 通讯作者: 白江平
  • 作者简介:E-mail: 1637835362@qq.com
  • 基金资助:
    国家自然科学基金项目(31660432);国家现代农业产业技术体系建设专项(CARS-09-P14);甘肃省马铃薯产业体系(GARS-03-P1);甘肃省创新能力提升项目(2019B-073);甘肃省科技计划项目(19ZD2WA002-02);甘肃省科技计划项目(18JR3RA174);甘肃农业大学国重实验室开放基金(GSCS-2017-9);甘肃农业大学创新基金项目(GAU-XKJS-2018-085)

Identification of StIgt gene family and expression profile analysis of response to drought stress in potato

QIN Tian-Yuan1,2(), LIU Yu-Hui1,2, SUN Chao1,2, BI Zhen-Zhen1,2, LI An-Yi3, XU De-Rong1,2, WANG Yi-Hao1,2, ZHANG Jun-Lian1, BAI Jiang-Ping1,2,*()   

  1. 1Gansu Provincial Key Lab of Aridland Crop Science / Gansu Key Laboratory of Crop Improvement & Germplasm Enhancement, Lanzhou 730070, Gansu, China
    2College of Agronomy, Gansu Agricultural University, Lanzhou 730070, Gansu, China
    3College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, Gansu, China
  • Received:2020-06-04 Accepted:2020-10-14 Published:2021-04-12 Published online:2020-11-06
  • Contact: BAI Jiang-Ping
  • Supported by:
    National Natural Science Foundation of China(31660432);China Agriculture Research System(CARS-09-P14);Gansu Province Potato Industry System(GARS-03-P1);Gansu Provincial Education Department(2019B-073);Gansu Provincial Science and Technology Department(19ZD2WA002-02);Gansu Provincial Science and Technology Department(18JR3RA174);Key Laboratory of Arid Land Crop Science of Gansu Agricultural University(GSCS-2017-9);Innovation Fund of Gansu Agricultural University(GAU-XKJS-2018-085)

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摘要：

干旱胁迫是影响马铃薯产量和品质的主要非生物胁迫因素之一。马铃薯在抵御干旱胁迫的过程中, 根系的生长发育和构型分布发挥着重要作用。Igt基因家族是普遍存在于植物中的一类功能基因, 在调控植物根系构型和提高植株抗逆性等方面效果显著。本研究以马铃薯双单倍体‘DM-v4.03’高质量基因组为参考, 在全基因组范围内分析鉴定了StIgt基因家族的成员, 并采用多种生物信息学软件对其进行了系统进化树构建、染色体定位、保守蛋白结构域、基因结构和顺式元件预测。同时, 利用本课题组前期对马铃薯四倍体品系在不同干旱条件下的转录组测序结果, 分析了StIgts响应干旱胁迫的表达谱。结果表明, 在马铃薯中共鉴定获得10个StIgt家族成员, 其中StIgt1由本课题组前期克隆获得。除StIgt1位置信息不明外, 其余基因不均匀地分布在1、2、5、7、10和11号染色体上。StIgt家族蛋白长度为110~283个氨基酸, 分子量介于13.136~32.542 kD之间, 预测等电点为3.82~9.86。系统进化树分析发现, 该基因家族可分为3个亚族, 亚族间的基因结构、蛋白保守域和顺式作用元件差别明显。干旱胁迫下的表达谱分析表明, StIgt6、StIgt7、StIgt9和StIgt10响应早期干旱胁迫, 在干旱2 h时即迅速上调表达。这些结果为阐明StIgt基因家族的进化关系和进一步研究其成员的功能特性提供了理论基础。

关键词: 全基因组, 马铃薯, StIgt基因家族, 干旱胁迫, 表达谱分析

Abstract:

Drought stress is one of the main abiotic stress factors affecting potato yield and quality. The root growth, development and architecture play an important role in potato drought resistance. The Igt gene family is a functional gene family that are ubiquitous in plants, and has significant effects in regulating root architecture and improving plant stress resistance. In this study, the potato double haploid ‘DM-v4.03’ high-quality genome was used as a reference, and members of the StIgt gene family were identified and analyzed on a genome-wide scale. Phylogenetic trees, chromosomal location, conservative protein domains, gene structure and cis element prediction were carried out by bioinformatics software. Meanwhile, the expression profiles of StIgts in response to drought stress were analyzed based on the transcriptome sequencing results of potato tetraploid lines under different drought conditions. The results showed that a total of 10 StIgt family members were identified in potato, of which StIgt1 was obtained and cloned by our research group. Except for the unknown position information of StIgt1, the remaining genes are unevenly distributed on chromosome 1, 2, 5, 7, 10, and 11. StIgts proteins range in length from 110 to 283 amino acids and have molecular weights ranging from 13.136 kD to 32.542 kD. The predicted isoelectric point is 3.82 to 9.86. Phylogenetic tree analysis revealed that the gene family can be divided into three subfamilies. The gene structure, protein conserved domains and cis-acting elements differ significantly among three subfamilies. The expression profile analysis under drought stress showed that StIgt6, StIgt7, StIgt9 and StIgt10 responded to the early drought stress which rapidly up-regulated at two hours of drought treatment. These results provide a theoretical basis for elucidating the evolutionary relationship of the StIgt gene family and further studying the functional characteristics of its members.

Key words: genome-wide, potato, StIgt gene family, drought stress, gene expression analyses

表1

拟南芥、桃、玉米与水稻Igt基因登录号"

基因名称
Gene name		 基因登录号
GenBank No.		 基因名称
Gene name		 基因登录号
GenBank No.
AtDro1 AT1G72490 AtLazy6 AT3G27025
AtDro2 AT1G19115 PpeLazy1 LOC18782538
AtDro3 AT1G17400 PpeLazy2 LOC18790006
OsDro1 BAN59748.1 ZmLazy LOC100193776
PpeDro1 LOC103327608 OsLazy1 LOC4350543
PpeDro2 LOC18770522 PpeTac1 LOC18773917
AtLazy1 AT5G14090 Ostac1 LOC4347655
AtLazy5 AT3G24750 AtTac1 AT2G46640

表2

马铃薯StIgt基因家族信息"

基因名称
Gene name		 基因ID
Gene ID		 亚组
Group		 染色体位置
Chromosome location		 大小
Size		 分子量
MW (kD)		 等电点
pI
StIgt10 PGSC0003DMG400042006 I chr11: 19822270-19822602 110 13,136.1 9.86
StIgt9 PGSC0003DMG400035280 I chr01: 12153224-12154281 139 15,371.1 4.61
StIgt8 PGSC0003DMG400020205 I chr02: 48071799-48076548 256 29,350.3 6.41
StIgt7 PGSC0003DMG401019811 I chr05: 15169941-15172623 255 27,797.7 5.41
StIgt6 PGSC0003DMG400041371 II chr07: 26993146-27002422 275 31,382.8 4.38
StIgt5 PGSC0003DMG400005529 II chr10: 5159202-5164672 281 32,338.2 5.15
StIgt4 PGSC0003DMG400022847 II chr01: 59885567-59885980 137 15,989.9 3.82
StIgt3 PGSC0003DMG400000126 II chr01: 73826302-73827911 221 24,499.6 5.38
StIgt2 PGSC0003DMG400016036 III chr07: 39417955-39420740 228 26,123 6.17
StIgt1 StDro1 III 未知Unknown 283 32,542.3 6.68

图1

基于马铃薯和其他物种Igt基因家族系统进化树"

图2

马铃薯StIgt基因家族成员的蛋白保守域和基因结构 A: 蛋白结构域特征; B: 基因结构。"

图3

马铃薯StIgts的染色体定位(A)和顺式作用元件分析(B) ABRE: 脱落酸作用元件; ERE: 参与防御和应激反应的作用元件; MBS: 干旱胁迫反应元件; TCA-element: 水杨酸作用元件; TGA-element: 生长素响应元件; W-box: 胁迫响应元件。"

图4

马铃薯StIgt基因在不同干旱胁迫时间下的表达模式分析"

图5

干旱胁迫时间下马铃薯StIgt1、StIgt3和StIgt10基因的表达 *, **分别表示在0.05和0.01水平上显著差异。"

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