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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (9): 1584-1589.doi: 10.3724/SP.J.1006.2009.01584

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Mapping of a Novel Semi-Sterile Pollen QTL in Rice

ZENG Bo1,LI Min1,YANG Zu-Yong2,TAN Chen-Ju1,DONG Hua-Lin1,YU Si-Bin1,*   

  1. 1College of Plant Science and Technology,Huazhong Agricultural University,Wuhan 430070,China;2Hubei Seed Group Company,Wuhan 430070,China
  • Received:2009-02-25 Revised:2009-05-05 Online:2009-09-12 Published:2009-07-03
  • Contact: YU Si-Bin, E-mail: ysb@mail.hzau.edu.cn

Abstract:

Hybrid sterility has been an obstacle in utilization of potential heterosis in inter-subspecific hybrids of indica and japonica.In order to understand the genetic basis of pollen sterility of indica-japonica hybrid, we identified a line (IL37) with semi-sterility from a set of chromosomal segment substitution lines, of which each contained a single or few substitution segments from a japonica variety Nipponbare in the genetic background of indica variety Zhenshan 97B. The graphical genotype analysis of the line (IL37) by using 160 polymorphic SSR revealed that there were three chromosomal segments from the japonica with the similar genetic background of Zhenshan 97B. Its derived F2 and F3 segregation populations were planted respectively in Wuhan and Hainan, and evaluated for quantitative trait loci (QTLs) conferring pollen fertility and spikelet fertility via single-marker analysis and interval mapping. One new QTL of pollen sterility was detected at the interval RM262–RM475 on chromosome 2, explaining the phenotypic variation of 13.9%. The results suggest that japonica substitution segment carrying the QTL is a major cause of the semi-sterility in IL37. Further fine mapping and identification of candidate genes in the QTL region would facilitate a better understanding of the genetic basis of pollen sterility in rice.

Key words: Chromosomal segment substitution line, Pollen semi-sterility, Quantitative trait loci, Rice(Oryza sativa L.)

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