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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (9): 1584-1589.doi: 10.3724/SP.J.1006.2009.01584


Mapping of a Novel Semi-Sterile Pollen QTL in Rice

ZENG Bo1,LI Min1,YANG Zu-Yong2,TAN Chen-Ju1,DONG Hua-Lin1,YU Si-Bin1,*   

  1. 1College of Plant Science and Technology,Huazhong Agricultural University,Wuhan 430070,China;2Hubei Seed Group Company,Wuhan 430070,China
  • Received:2009-02-25 Revised:2009-05-05 Online:2009-09-12 Published:2009-07-03
  • Contact: YU Si-Bin, E-mail: ysb@mail.hzau.edu.cn


Hybrid sterility has been an obstacle in utilization of potential heterosis in inter-subspecific hybrids of indica and japonica.In order to understand the genetic basis of pollen sterility of indica-japonica hybrid, we identified a line (IL37) with semi-sterility from a set of chromosomal segment substitution lines, of which each contained a single or few substitution segments from a japonica variety Nipponbare in the genetic background of indica variety Zhenshan 97B. The graphical genotype analysis of the line (IL37) by using 160 polymorphic SSR revealed that there were three chromosomal segments from the japonica with the similar genetic background of Zhenshan 97B. Its derived F2 and F3 segregation populations were planted respectively in Wuhan and Hainan, and evaluated for quantitative trait loci (QTLs) conferring pollen fertility and spikelet fertility via single-marker analysis and interval mapping. One new QTL of pollen sterility was detected at the interval RM262–RM475 on chromosome 2, explaining the phenotypic variation of 13.9%. The results suggest that japonica substitution segment carrying the QTL is a major cause of the semi-sterility in IL37. Further fine mapping and identification of candidate genes in the QTL region would facilitate a better understanding of the genetic basis of pollen sterility in rice.

Key words: Chromosomal segment substitution line, Pollen semi-sterility, Quantitative trait loci, Rice(Oryza sativa L.)

[1] Sano Y. Rice Genetics. Manila: International Rice Research Institute, 1986. pp 109-118

[2] Kato S, Kosaka H, Hara S. On the affinity of rice varieties as shown by fertility of hybrid plants. Bulletin of Sciences of Faculty of Agriculture, Kyushu University, 1928, 3: 132-147

[3] Chaudhary R C, Virmani S S, Khush G S. Patterns of pollen abortion in some cytoplasmic-genetic male sterile lines of rice. Oryza, 1981, 18: 140-142

[4] Lu C G, Takabatake K, Ikehashi H. Identification of segregation distortion neutral alleles to improve pollen fertility of indica-japonica hybrids in rice (Oryza sativa L.). Euphytica, 2000, 113: 101-107

[5] Lu Q, Li X H, Guo D, Xu C G, Zhang Q. Localization of pms3, a gene for photoperiod-sensitive genic male sterility, to a 28.4-kb DNA fragment. Mol Gen Genomics, 2005, 273: 507-511

[6] Zhao Z G, Wang C M, Jiang L, Zhu S S, Ikehashi H, Wan J. Identification of a new hybrid sterility gene in rice (Oryza sativa L.). Euphytica, 2006, 151: 331-337

[7] Li D T, Chen L M, Jiang L, Zhu S S, Zhao Z G, Liu S J, Su N, Zhai H Q, Ikehashi H, Wan J M. Fine mapping of S32(t), a new gene causing hybrid embryo sac sterility in a Chinese landrace rice (Oryza sativa L.). Theor Appl Genet, 2007, 100: 697-712

[8] Yang-J(杨杰), Zhai H-Q(翟虎渠), Wang C-L(王才林), Zhong W-G(仲维功), Zou J-S(邹江石), Ikehashi H(池桥宏), Wan J-M(万建民). QTL analysis of low-temperature-sensitive pollen sterility in indica-japonica hybrid rice (Oryza sativa L.). Acta Genet Sin (遗传学报), 2005, 32(5): 507-513(in Chinese with English abstract)

[9] Hu F Y, Xu P, Deng X N, Zhou J W, Li J, Tao D Y. Molecular mapping of a pollen killer gene S29(t) in Oryza glaberrima and co-linear analysis with S22 in O. glumaepatula. Euphytica, 2006, 151: 273-278

[10] Li W, Zeng R, Zhang Z, Ding X, Zhang G. Identification and fine mapping of S-d, a new locus conferring the partial pollen sterility of intersubspecific F1 hybrids in rice (Oryza sativa L.). Theor Appl Genet, 2008, 116: 915-922

[11] Liu X, Wang S W, Wang Y, Wei S. Genetic analysis and molecular mapping of a nuclear recessive male sterility gene, ms91(t), in rice. Genome, 2007, 50: 796-801

[12] Li W C, Jiang L, Zhou S R, Wang C M, Liu L L, Chen L M, Ikehashi H, Wan J M. Fine mapping of pss1, a pollen semi-sterile gene in rice (Oryza sativa L.). Theor Appl Genet, 2007, 114: 939-946

[13] Jing W, Zhang W W, Jiang L, Chen L M, Zhai H Q, Wan J M. Two novel loci for pollen sterility in hybrids between the weedy strain Ludao and the japonica variety Akihikari of rice (Oryza sativa L.). Theor Appl Genet, 2007, 114: 915-925

[14] Chen J, Ding J, Ouyang Y, Du H, Yang J, Cheng K, Zhao J, Qiu S, Zhang X, Yao J, Liu K, Wang L, Xu C, Li X, Xue Y, Xia M, Ji Q, Lu J, Xu M, and Zhang Q. A triallelic system of S5 is a major regulator of the reproductive barrier and compatibility of indica-japonica hybrids in rice. Proc Natl Acad Sci USA, 2008, 105: 11436-11441

[15] Yu S-B(余四斌), Mu J-X(穆俊祥), Zhao S-J(赵胜杰), Zhou H-J(周红菊), Tan Y-B(谭友斌), Xu C-G(徐才国), Luo L-J(罗利军), Zhang Q-F(张启发). Development and selection of introgression lines with the identical genetic background of varieties Zhenshan 97B and 9311. Mol Plant Breed (分子植物育种), 2005, 3(5): 629-636 (in Chinese with English abstract)

[16] Li Z-B(李泽炳). A preliminary discussion about the classification of male sterile lines of rice in China. Acta Agron Sin (作物学报), 1980, 6(1): 17-26 (in Chinese)

[17] Rogers S O, Bendich A J. Extraction of DNA from Plant Tissue. Plant Mol Biol Manual, A6. Netherlands: Kluwer Academic Publishers, 1988.pp1210

[18] McCouch S, Teytelman L, Xu Y, Lobos K B, Clare K, Walton M, Fu B, Maghirang R, Li Z, Xing Y, Zhang Q, Kono I, Yano M, Fjellstrom R, DeClerck G, Schneider D, Cartinhour S, Ware D, Stein L. Development and mapping of 2240 new SSR markers for rice (Oryza sativa L.). DNA Res, 2002, 9: 199-207

[19] Lincoln S E, DaIy M J, Lander E S. Constructing Genetics Maps with MapMaker/EXP3.0. Whitehead Institute Technical Report. Cambridge: MA, 1992

[20] Brant J B, Gustavo C A, Peter M G. Fast and sensitive silver staining of DNA in polyacrylamide gels. Anal Biochem, 1991, 196: 80-82

[21] StatSoft. Statistica. StatSoft Incorporated, Tusla, Oklahoma, 1997

[22] Wang D L, Zhu J, Li Z K, Paterson A H. Mapping QTLs with epistatic effects and QTL by environment interactions by mixed linear model approaches. Theor Appl Genet, 1999, 99: 1255-1264

[23] McCouch S R, Cho Y G, Yano M, Paul E, Blinstrub M, Morishima H, Kinoshita T. Report on QTL nomenclature. Rice Genet Newsl, 1997, 14: 11-13

[24] Ali A J, Xu J L, Ismail A M, Fu B Y, Vijaykumar C H M, Gao Y M, Domingo J, Maghirang R, Yu S B, Gregorio G, Yanaghihara S, Cohen M, Carmen B, Mackill D, Li Z K. Hidden diversity for abiotic and biotic stress tolerances in the primary gene pool of rice revealed by a large backcross breeding program. Field Crops Res, 2006, 97: 66-76

[25] Li H B, Wang J, Liu A M, Liu K D, Zhang Q, Zou J S. Genetic basis of low-temperature-sensitive sterility in indica-japonica hybrids of rice as determined by RFLP analysis. Theor Appl Genet, 1997, 95: 1092-1097

[26] Kubo T, Yoshimura A. Epistasis underlying female sterility detected in hybrid breakdown in an indica-japonica cross of rice (Oryza sativa L.). Theor Appl enet,2005, 110: 346-355

Long Y, Zhao L, Niu B, Su J, Wu H, Chen Y, Zhang Q, Guo J, Zhuang C, Mei M, Xia J, Wang L, Wu H, Liu Y. Hybrid male sterility in rice controlled by interaction between divergent alleles of two adjacent genes. Proc Natl Acad Sci USA, 2008, 105: 18871-18876

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