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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (07): 1247-1252.doi: 10.3724/SP.J.1006.2012.01247


Mapping QTL for Cell Membrane Permeability of Leaf Treated by Low Temperature in Winter Wheat

JU Wei1,YANG Cai-Feng1,ZHANG Shu-Hua2,TIAN Ji-Chun3,HAI Yan4,YANG Xue-Ju2,*   

  1. 1 College of Agronomy, Agricultural University of Hebei / Crop Germplasm Laboratory of Hebei Province , Baoding 71000, China; 2 College of Life Sciences, Agricultural University of Hebei, Baoding 71000, China; 3 College of Agronomy, Shandong Agricultural University, Tai’an 271018, China; 4 Henan Key Laboratory for Crop Improvement, Zhengzhou 450002, China
  • Received:2011-12-26 Revised:2012-04-16 Online:2012-07-12 Published:2012-05-11
  • Contact: 杨学举, E-mail: shmyxj@hebau.edu.cn, Tel: 0312-7528267

Abstract: To understand the genetic mechanism of cold resistance in winter wheat, we mapped quantitative trait loci (QTLs) for cellmembrane permeability of leaf treated by low temperature using compound interval method based on an existing linkage map containing 324 simple sequence repeat (SSR) markers. The map population, which was consisted of 168 double haploid lines from the cross Huapei 3 × Yumai 57, was planted in three sites, and the cellmembrane permeability of leaf treated by low temperature (−18°C) was determined with electric conductometry . A total of 21 additive QTLs associated with cell membrane permeability of leaf were detected in three environments, which were distributed on chromosomes 1B, 2A,3A, 3B, 5B, 6A, 6B, 6D, 7B, and 7D. Four loci (qCMP-1B-1, qCMP-3B-2, qCMP-5B-1, and qCMP-5B-4) with phenotypic contributions higher than 10% were regarded as major genes, and the remaining QTLs were minor genes with phenotypic contributions lower than 10%. A common locus in the Xgwm213–Xswes861.2 interval on chromosome 5B was detected in the three environments, with 0 cM genetic distance from Xswes861.2. The loci, designated qCMP-5B-1 in environment 1 and qCMP-5B-4 in environment 3, explained 17.5% and 14.0% with phenotypic variations, respectively. The result of the present study was useful for marker-assisted selection and breeding for cold resistance of wheat.

Key words: Winter wheat, DH population, Cell membrane permeability, Cold resistance, Quantitative trait loci

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