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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (9): 1662-1671.doi: 10.3724/SP.J.1006.2009.01662


Leaf Stay-Green Traits in Chinese Maize Inbred Lines and Their Relationship with Grain Yield

LIU Kai-Chang1,DONG Shu-Ting2,ZHAO Hai-Jun1,WANG Qing-Cheng1,*,LI Zong-Xin1,LIU Xia1,ZHANG Hui1   

  1. 1 Maize Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, China; 2 State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, China
  • Received:2008-12-31 Revised:2009-04-23 Online:2009-09-12 Published:2009-07-04
  • Contact: WANG Qing-Cheng, E-mail: qcwang@saas.ac.cn; Tel: 0531-83179402


Leaf stay-green trait is one of the major targets in maize (Zea mays L.) breeding. Although sugar concentration in culm, green leaf number, leaf area, and chlorophyll content at maturity have been used to evaluate the stay-green trait of maize in earlier studies, there is no a common criterion. To further study the evaluation system for stay-green in maize and disclose the relationship of stay-green with grain yield, we sampled 75 inbred lines from common parents of Chinese maize cultivars. At silking stage and 10, 20, 30, 40, 50, and 60 d after silking, the leaf area (LA), leaf area duration (LAD), relative leaf area, and relative green-leaf area (RGLA) were measured. The results showed that the changes of RGLA after silking accorded with the equation of y = aeb-cx/ (1+eb-cx). On the basis of correlation analysis, the green leaf number, chlorophyll content at physiological maturity, and mean decreasing rate of RGLA (Vm) were selected as the key indices to discriminate the stay-green and non-stay-green maize genotypes. According to relative green leaf area at physiological maturity (MRGLA), the maximum decrease rate of RGLA (Vmax), and the mean decreasing rate of RGLA (Vm), the 75 inbred lines were classified into stay-green and non-stay-green two types with Hiechical clustering analysis. The stay-green type was composed of 12 inbred lines with the following common characteristics: MRGLA of more than 60%, Vm of 0.687% d-1, no significant decrease of RGLA during the whole growing period (67.07% at maturity), and high green leaf number (8.8 leaves) and chlorophyll content (4.43 mg dm-2) at physiological maturity. The non-stay-green type, consisting of 63 inbred lines, was further categorized with quick-leaf-senescence (50 inbred lines) and slow-leaf-senescence (13 inbred lines) subgroups. In the quick- and slow-leaf senescence subgroups, the RGLA at maturity, Vm, green leaf number at maturity, and chlorophyll content at maturity were 17.75% and 33.55%, 1.89% d-1 and 1.44% d-1, 3.2 and 6.2, and 2.06 mg dm-2 and 3.17 mg dm-2, respectively. At physiological maturity, the RGLA was positively correlated with LAD (r= 0.8861, P < 0.01) and yield per plant (r = 0.8221, P < 0.01). The LAD and yield per plant were 20.02–23.87% and 50.44–59.38% higher in the stay-green type than in the non-stay-green type, respectively. Thus, the stay-green type had higher yield potential due to larger green leaf area, higher chlorophyll content and photosynthesis efficiency as well as longer photosynthesis duration.

Key words: Maize, Inbred line, Stay-green, Yield

[1] Pan R-C(潘瑞炽). Plant Physiology (植物生理学). Beijing: Higher Education Press, 2004 (in Chinese)

[2] Thomas H, Howarth C J. Five ways to stay green. J Exp Bot, 2000, 51: 329-337

[3] Subudhi P K, Rosenow D T, Nguyen H T. Quantitative trait loci for the stay-green trait in sorghum (Sorghum bicolor L. Moench): Consistency across genetic backgrounds and environments. Theor Appl Genet, 2000, 101: 733-741

[4] Haussmann B I G, Mahalakshmi V, Reddy B V S. QTL mapping of stay-green in two sorghum recombinant inbred populations. Theor Appl Genet, 2002, 106: 133-142

[5] Tollenaar M, Daynard T B. Leaf senescence in short-season maize hybrids. Can J Plant Sci, 1978, 58: 869-874

[6] Ceppi D, Sala M, Gentinetta E. Genotype-dependent leaf senescence in maize: 1. Inheritance and effects of pollination-prevention. Plant Physiol, 1987, 85: 720-725

[7] Craft-brandner S J, Below F E, Wittenbach V A. Effect of ear removal on CO2 exchange and activties of ribulose bisphate earboxylase and phosphoenlpyruvate carboxylase of maize hybrids and inbred lines. Plant Physiol, 1987, 84: 261-265

[8] Phillips D A, Pierce R O, Edie S A, Foster K A, Knowles P F. Delay leaf senescence in soybean. Crop Sci, 1984, 24: 518-522

[9] Thomas H, Smart C M. Crops that stay green. Ann Appl Biol, 1993, 123: 193-219

[10] Xu W W, Rosenow D T, Nguyen H T. Stay-green trait in grain sorghum: relationship between visual rating and leaf chlorophyll concentration. Plant Breed, 2000, 119: 365-367

[11] Borrell A K, Hammer G H. Does maintaining green leaf area in sorghum improve yield under drought? II. Dry matter production and yield. Crop Sci, 2000, 40: 1037-1048

[12] Gentinetta E, Ceppi D, Lepori C, Perico G, Motto M, Salamini F. A major gene for delayed senescence in maize: Pattern of photosynthates accumulation and inheritance. Plant Breed, 1986, 97: 193-203

[13] Duncan R R. The association of plant senescence with root and stalk diseases in sorghum. In: Mughogho L K, Rosenberg G, eds. Sorghum Root and Stalk Rots: A Critical Review. Proceedings of the Consultative Group Discussion on Research Needs and Strategies for Control of Sorghum Root and Stalk Rot Diseases. Bellagio, Italy & Patancheru, India: ICRISAT, 1984. pp 99-110

[14] Guo Q-F(郭庆法), Wang Q-C(王庆成), Wang L-M(汪黎明). Maize Cultivation in China (中国玉米栽培学). Shanghai: Shanghai Scientific and Technical Publishers, 2004. pp 32-34 (in Chinese)

[15] Dong S-T(董树亭), Wang K-J(王空军), Hu C-H(胡昌浩).Development of canopy apparent photosynthesis among maize varieties from different eras. Acta Agron Sin (作物学报), 2000, 26(2): 200-204 (in Chinese with English abstract)

[16] Duvick D N. Genetic rates of grain in hybrid maize yields during the past 40 years. Maydica, 1997, 23: 187-196

[17] Russell W A. Registration of B93 and B94 inbred lines of maize. Crop Sci, 1991, 31: 247-248

[18] Choi K J, Chin M S, Park K Y, Kim S L, Chung T W, Lee H S. Segregation of stay-green characters in an F2 population. Maize Genetics Cooperation Newsl, 1995, 69: 123

[19] Chfistens L E, Below F E. Hagreman R H. The effect of ear removal on sevwscence and metabolism of maize. Plant Physiol, 1981, 68: 1180-1185

[20] Bekavac G. Path analysis of stay-green trait in maize. Cereal Res Commun, 1998, 26: 161-167

[21] Walulu R S, Darrell T R, Wester D B, Nguyen H T. Inheritance of the stay green trait in sorghum. Crop Sci, 1994, 34: 970-972

[22] Waggoner P E, Berger R D. Defoliation, disease, and growth. Phytioathology, 1987, 77: 393-398

[23] Wang J-G(王建国), Du G-J(杜桂娟), Cai J-X(蔡纪新), Zhang B-S(张宝石). Relationship between stay-green and other agronomic traits in maize. Rain Fed Crops (杂粮作物), 2003, 23(6): 336-339 (in Chinese with English abstract)

[24] Craft-Brandner S J, Below F E, Wittenbach V A, Harper J E, Hageman R H. Differential senescence of maize hybrids following ear removal: II. Selected leaf. Plant Physiol, 1984, 74: 368-373

[25] Yang J-P(杨俊品), Rong T-Z(荣廷昭), Xiang D-Q(向道权), Tang H-T(唐海涛), Huang L-J(黄烈健), Dai J-R(戴景瑞). QTL mapping of quantitative traits in maize. Acta Agron Sin (作物学报), 2005, 31(2): 188-196 (in Chinese with English abstract)

[26] Liu K-C(刘开昌), Wang Q-C(王庆成), Zhang H-S(张海松), Feng K(冯凯). Advance in research of physiological mechanism and genetic traits of stay-green of maize. Shandong Agric Sci (山东农业科学), 2003, (2): 48-51(in Chinese with English abstract)

[27] Zheng H-J(郑洪建), Shen X-F(沈雪芳), Wu A-Z(吴爱忠). The research advance in inheritance of stay-green traits of maize leaf blades.Acta Agric Shanghai(上海农业学报), 2007, 23(4): 90-94 (in Chinese with English abstract)

[28] Zhu Z-P(朱治平). Laboratory Manual of Plant Physiology (植物生理学实验手册). Shanghai: Shanghai Scientific and Technical Publishers, 1985 (in Chinese)

[29] Oosterom E J van, Jayachandran R, Bidinger F R. Diallel analysis of the stay green trait and its components in sorghum. Crop Sci, 1996, 36: 549-555

[30] Nguyen H T, Xu W W, Rosenow D T, Mullet J E, McIntyre L. Use of biotechnology in sorghum drought resistance breeding. In: Proceedings of the International Conference on Genetic Improvement of Sorghum and Pear Millet, Lubbock, Texas, USA, 1996. pp 412-424

[31] Crasta O R, Xu W W, Rosenow D T, Mullet J E, Nguyen H T. Mapping of post-flowering drought resistance traits in grain sorghum: Association of QTLs influencing senescence and maturity. Mol Gen Genet, 1999, 262: 579-588

[32] Wan C, Xu W W, Sosebee R E, Machado S, Archer T. Hydraulic lift in drought-tolerant and -susceptible maize hybrids. Plant & Soil, 2000, 219: 117-126

[33] Thomas H, Morgan W G, Thomas A M, Ougham H J. Expression of the stay-green character introgressed into Lolium temulentum Ceres from a senescence mutant of Festuca pratensis. Theor Appl Genet, 1999, 99: 92-99

[34] Smart C M, Hosken S E, Thomas H, Greaves J A, Blair B G, Schuch W. The timing of maize leaf senescence and characterisation of senescence-related cDNAs. Physiol Plant, 1995, 93: 673-682

[35] Willman M R, Below F E, Lambert R J, Howey A E, Mies D W. Plant traits related to productivity of maize: II. Development of multiple trait models. Crop Sci, 1987, 27: 1122-1126

[36] Dong S-T(董树亭), Gao R-Q(高荣岐), Hu C-H(胡昌浩), Wang Q-Y(王群瑛), Wang K-J(王空军).Study of canopy photosynthesis property and high yield potential after anthesis in maize. Acta Agron Sin (作物学报), 1997, 23(3): 318-325 (in Chinese with English abstract)

[37] Wada Y, Wada G. Varietal difference in leaf senescence during ripening period of advanced indica rice. Jpn J Crop Sci, 1991, 60: 529-536

[38] Bolanos J, Edmeades G O. The importance of the anthesis-silking interval in breeding for drought tolerance in tropical maize. Field Crops Res, 1996, 48: 65-80
Jiang G H, He Y Q, Xu C G, Li X H, Zhang Q. The genetic basis of stay-green in rice analyzed in a population of doubled haploid lines derived from an indica by japonica cross. Theor Appl Genet, 2004, 108: 688-698
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