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Acta Agronomica Sinica ›› 2022, Vol. 48 ›› Issue (3): 747-758.doi: 10.3724/SP.J.1006.2022.11019

• RESEARCH NOTES • Previous Articles     Next Articles

Application of Fhb1 gene in wheat breeding programs for the Yellow-Huai Rivers valley winter wheat zone of China

MA Hong-Bo(), LIU Dong-Tao*(), FENG Guo-Hua, WANG Jing, ZHU Xue-Cheng, ZHANG Hui-Yun, LIU Jing, LIU Li-Wei, YI Yuan   

  1. Xuzhou Institute of Agricultural Sciences in Jiangsu Xuhuai Area, Xuzhou 221131, Jiangsu, China
  • Received:2021-03-01 Accepted:2021-07-21 Online:2021-08-10 Published:2021-08-10
  • Contact: LIU Dong-Tao E-mail:mahongbo863@163.com;liudongtao317@163.com
  • Supported by:
    Key Research and Development Program (Modern Agriculture) of Xuzhou(KC20036);Key Research and Development Program of Jiangsu Province(BE2018340-2);China Agriculture Research System of MOF and MARA(CARS-03)

Abstract:

Fusarium head blight (FHB), caused by Fusarium graminearum, is an important disease that seriously threatens the safety of wheat production. Breeding progress of resistant cultivars has been limited due to absence of the resistant sources with facultative growth habit and poor screening environment in the Huang-Huai region, the main wheat producing area of China. Fhb1, as the most effective and stable gene, is the only gene widely used in the global breeding programs for improving FHB resistance of wheat. However, utilization of Fhb1 in the Yellow-Huai River Valleys Winter Wheat Zone is limited so far. In this study, several excellent advanced lines with Fhb1 were developed by limited backcrossing and molecular marker-assisted selection in the BC2F1 progenies of the susceptible variety Aikang 58 (the recurrent parent) and H35 (the donor parent of Fhb1 gene). Then, the individuals were used to cross with Xumai 36 and Xumai 2023 (with high yield but susceptible to FHB), and a series of elite lines with Fhb1 were developed using the doubled haploid and the traditional pedigree selection methods. Xumai DH9 and Xumai 17252 were moderate resistance to FHB in different screening environments for several years. The average resistance level of the lines with Fhb1 gene was significantly higher than that of the susceptible control. The introduction of Fhb1 significantly improved the resistance to FHB, but some lines were still highly susceptible, indicating that the resistance to FHB was affected by other genetic factors. This study provides an example for the use of Fhb1 gene in improving FHB resistance in the Yellow-Huai River Valleys Winter Wheat Zone of China.

Key words: Triticum aestivum, Fhb1 gene, molecular marker-assisted selection, breeding

Fig. 1

Process of developing new wheat lines with FHB resistance by the pedigree selection and doubled haploid methods"

Table 1

Information of primers used in this study"

标记
Marker
引物序列
Primer sequence (5′-3′)
扩增产物大小
Size of target fragment
参考文献
Reference
Gwm493 F: TTCCCATAACTAAAACCGCG
R: GGAACATCATTTCTGGACTTTG
Fhb1 180 bp
nonFhb1 140 bp
[7]
JAAS01 F: GTTCCACGTCTTCTTACATAATCCC
R: TGAAGTTCATGCCACGCATA
Fhb1 236 bp
nonFhb1 -
专利号:
Patent No. ZL201310013279.2
TaHRC-GSM GSM-F: ATTCCTACTAGCCGCCTGGT
GSM-R: ACTGGGGCAAGCAAACATTG
Fhb1 1300 bp
nonFhb1 2000 bp
[25]

Fig. 2

Genotype of some BC1F1 plants detected by marker Gwm493 M: marker; Lane 1: H35; Lane 2: Aikang 58; Lanes 4, 7, 9, 10, 12, 14, 15, and 18 indicate plants without Fhb1 gene; Lanes 3, 5, 6, 8, 11, 13, 16, 17, 19, and 20 indicate plants with heterozygous Fhb1 gene."

Fig. 3

Genotype of some F1 plants detected by marker JAAS01 M: marker; Lanes 1, 2, 3, 4, 6, 9, and 11 indicate plants with Fhb1 gene; Lanes 5, 7, 8, 10, and 12-17 indicate plants without Fhb1 gene."

Fig. 4

Genotype of some F2 plants detected by codominance marker TaHRC-GSM M: marker; Lanes 1, 6-10, 14, and 15 indicate plants with homozygous Fhb1 gene; Lanes 4, 12, 13, and 16 indicate plants without Fhb1 gene; Lanes 2, 3, 5, and 11 indicate plants with heterozygous Fhb1 gene."

Table 2

Summary of FHB resistance of lines during 2019-2020 plant season"

品系/组合
Line/Cross
家系数量
Number of lines
土表接种(徐州)
Soil surface inoculation
(in Xuzhou)
单花滴注(徐州)
Single flower drip inoculation
(in Xuzhou)
自然发病(建阳)
Screening under natural condition (in Jianyang)
病情指数
Disease index
范围
Range
病小穗率
Diseased spikelet rate (%)
范围
Range
病情指数
Disease index
范围
Range
H35/矮抗58*3//徐麦36
H35/Aikang58*3//Xumai 36
83 52.58±11.47 c 25.00-81.25 22.90±3.91 c 12.00-58.12 65.80±6.00 bc 51.92-79.17
徐麦36 Xumai 36 2 66.59±4.27 b 64.49-70.67 51.86±10.24 ab 44.62-59.10 67.73±3.85 b 65.00-70.25
H35/矮抗58*3//徐麦2023
H35/Aikang 58*3//Xumai 2023
83 43.31±12.83 cd 26.67-78.33 16.46±3.77 cde 7.77-63.71 66.18±5.59 b 52.08-80.77
徐麦2023 Xumai 2023 2 64.59±4.12 b 61.67-67.50 45.10±6.73 b 40.34-49.86 71.67±1.18 ab 70.83-72.50
徐农029 Xunong 029 24 39.32±6.54 de 26.25-50.00 21.43±6.03 c 12.66-42.06 60.24±5.52 c 47.73-72.73
淮麦20 Huaimai 20 2 55.35±3.68 cd 51.46-57.24 28.72±2.33 c 26.56-30.88
矮抗58 Aikang 58 2 79.17±29.47 a 58.33-100.00 49.22±8.53 b 43.18-55.25 79.17±11.79 a 70.83-87.50
徐麦35 Xumai 35 24 68.40±13.40 b 47.73-95.00 55.45±16.53 a 27.47-83.44 69.27±5.44 b 57.69-82.14

Fig. 5

FHB resistance of line 23 (b) from H35/Aikang 58*3// Xumai 36, CK lines Xumai 35 (a) and Xunong 029 (c) screening with single flower drip inoculation in Xuzhou"

Table 3

FHB resistance of homozygous lines with Fhb1 gene screening with three different methods during 2019-2020 plant season"

家系号
No. of family
H35/矮抗58*3//徐麦36 H35/Aikang58*3//Xumai36 H35/矮抗58*3//徐麦2023 H35/Aikang58*3//Xumai2023
土表接种(徐州)
Soil surface inoculation
(in Xuzhou)
单花滴注(徐州)
Single flower drip
Inoculation (in Xuzhou)
自然发病(建阳)
Screening under natural condition (in Jianyang)
家系号
No. of family
土表接种(徐州)
Soil surface inoculation
(in Xuzhou)
单花滴注(徐州)
Single flower drip inoculation
(in Xuzhou)
自然发病(建阳)
Screening under natural condition (in Jianyang)
病情指数Disease index 抗性评价Evaluation
of resistance
病小穗率Diseased
spikelet rate (%)
抗性评价Evaluation of resistance 病情指数Disease index 抗性评价Evaluation of resistance 病情指数
Disease index
抗性评价Evaluation of resistance 病小穗率
Diseased
spikelet rate (%)
抗性评价Evaluation of resistance 病情指数
Disease index
抗性评价Evaluation of resistance
1 25.00 MR 18.20 MR 51.92 MS 1 27.50 MR 11.64 MR 52.08 MS
2 50.00 MS 19.01 MR 68.18 S 2 29.17 MR 11.85 MR 77.08 S
3 57.50 S 24.40 MS 72.73 S 3 44.17 MS 14.18 MR 56.82 MS
4 43.33 MS 20.03 MR 62.50 MS 4 33.75 MR 16.44 MR 60.00 MS
5 40.00 MS 17.04 MR 62.50 MS 5 42.50 MS 11.43 MR 66.67 S
6 38.33 MR 32.62 S 69.23 S 6 40.00 MS 19.02 MR 67.50 S
7 47.50 MS 22.91 MS 67.50 S 7 50.83 MS 13.39 MR 62.50 MS
8 54.17 MS 21.87 MR 59.09 MS 8 45.00 MS 12.96 MR 80.77 S
9 49.17 MS 18.39 MR 60.00 MS 9 40.83 MS 24.23 MS 73.21 S
10 53.33 MS 17.61 MR 67.50 S 10 29.17 MR 9.72 MR 64.29 MS
11 56.67 S 17.13 MR 70.00 S 11 40.00 MS 12.49 MR 64.58 MS
12 53.33 MS 20.06 MR 60.00 MS 12 35.83 MR 10.71 MR 70.83 S
13 53.33 MS 23.68 MS 65.00 MS 13 38.33 MR 12.57 MR 62.50 MS
14 54.17 MS 17.84 MR 62.50 MS 14 35.83 MR 12.99 MR 61.54 MS
15 50.83 MS 19.99 MR 67.50 S 15 30.83 MR 11.43 MR 62.50 MS
16 58.33 S 22.15 MS 65.00 MS 16 31.67 MR 12.30 MR 65.38 S
17 60.83 S 22.80 MS 65.00 MS 17 29.17 MR 8.18 MR 62.50 MS
18 60.00 S 16.24 MR 60.00 MS 18 33.33 MR 10.10 MR 52.08 MS
19 56.67 S 21.31 MR 60.00 MS 19 36.67 MR 10.80 MR 62.50 MS
20 60.00 S 23.07 MS 57.50 MS 20 58.33 S 12.34 MR 57.50 MS
21 59.17 S 19.92 MR 57.50 MS 21 49.17 MS 14.00 MR 62.50 MS
22 49.17 MS 21.27 MR 62.50 MS 22 51.67 MS 21.11 MR 65.00 MS
23 38.33 MR 16.46 MR 60.00 MS 23 42.50 MS 15.74 MR 65.00 MS
24 61.67 S 35.43 S 75.00 S 24 40.83 MS 17.04 MR 63.64 MS
25 56.67 S 18.77 MR 75.00 S 25 37.50 MR 10.35 MR 66.67 S
26 55.00 MS 17.19 MR 68.18 S 26 33.33 MR 12.77 MR 68.75 S
27 45.00 MS 17.26 MR 65.91 S 27 30.83 MR 16.01 MR 70.83 S
28 46.67 MS 19.90 MR 56.82 MS 28 34.17 MR 14.31 MR 67.50 S
29 75.83 S 28.62 MS 70.83 S 29 34.17 MR 16.48 MR 65.91 S
30 81.25 S 32.19 S 68.75 S 30 30.83 MR 16.93 MR 66.67 S
31 75.00 S 39.30 S 65.38 S 31 47.50 MS 40.81 S 70.83 S
32 57.76 S 22.27 MS 66.67 S 32 47.50 MS 18.55 MR 66.67 S
33 49.14 MS 24.87 MS 67.50 S 33 60.00 S 18.31 MR 75.00 S
34 57.76 S 27.04 MS 73.08 S 34 45.83 MS 13.65 MR 66.67 S
35 75.83 S 24.61 MS 67.50 S 35 49.17 MS 12.37 MR 70.00 S
36 44.17 MS 18.40 MR 57.50 MS 36 54.17 MS 15.79 MR 68.18 S
37 35.00 MR 21.04 MR 57.50 MS 37 78.33 S 25.67 MS 67.50 S
38 37.50 MR 21.30 MR 55.00 MS 38 78.33 S 25.41 MS 70.00 S
39 44.17 MS 22.84 MS 56.82 MS 39 71.67 S 18.10 MR 70.83 S
40 40.00 MS 17.61 MR 72.73 S 40 47.50 MS 16.70 MR 75.00 S
41 35.83 MR 19.64 MR 66.67 S 41 60.83 S 16.66 MR 65.91 S
42 40.83 MS 15.19 MR 65.91 S 42 50.83 MS 15.77 MR 60.00 MS
43 44.17 MS 26.64 MS 68.75 S 43 65.83 S 15.39 MR 62.50 MS
44 45.00 MS 20.29 MR 79.17 S 44 60.83 S 25.12 MS 70.00 S
45 43.33 MS 19.71 MR 65.91 S 45 45.00 MS 15.68 MR 69.23 S
46 55.00 MS 15.37 MR 72.50 S 46 46.67 MS 16.57 MR 67.50 S
47 37.50 MR 13.78 MR 72.50 S 47 46.67 MS 17.89 MR 67.50 S
48 61.67 S 49.82 S 68.18 S 48 55.83 S 24.25 MS 67.50 S
49 48.33 MS 27.89 MS 66.67 S 49 61.67 S 25.24 MS 75.00 S
50 56.67 S 24.07 MS 67.50 S 50 27.50 MR 15.42 MR 61.36 MS
51 50.00 MS 22.22 MS 70.45 S 51 28.33 MR 14.49 MR 55.00 MS
52 37.93 MR 21.07 MR 55.00 MS 52 38.33 MR 15.82 MR 52.50 MS
53 53.33 MS 19.21 MR 55.00 MS 53 42.50 MS 28.78 S 55.00 MS
54 59.17 S 15.34 MR 57.50 MS 54 36.67 MR 10.30 MR 55.00 MS
55 55.83 S 23.10 MS 66.67 S 55 26.67 MR 12.11 MR 67.50 S
56 60.83 S 27.13 MS 65.91 S 56 28.33 MR 16.82 MR 70.00 S
57 64.17 S 20.17 MR 61.36 MS 57 38.33 MR 15.99 MR 67.50 S
58 63.33 S 25.74 MS 62.50 MS 58 28.33 MR 14.85 MR 68.18 S
59 61.67 S 17.84 MR 67.50 S 59 34.17 MR 19.04 MR 67.31 S
60 65.83 S 19.71 MR 67.50 S 60 31.67 MR 12.68 MR 69.23 S
61 63.33 S 21.87 MR 70.00 S 61 36.67 MR 15.06 MR 72.73 S
62 57.50 S 18.09 MR 70.00 S 62 34.17 MR 12.71 MR 66.67 S
63 54.17 MS 26.81 MS 62.50 MS 63 38.33 MR 14.84 MR 72.73 S
64 44.17 MS 22.17 MS 62.50 MS 64 55.00 MS 27.16 MS 67.50 S
65 55.83 S 18.44 MR 62.50 MS 65 33.33 MR 12.55 MR 68.18 S
66 50.83 MS 19.39 MR 68.75 S 66 35.00 MR 14.09 MR 67.50 S
67 56.67 S 18.71 MR 67.50 S 67 30.83 MR 12.85 MR 75.00 S
68 60.00 S 12.00 MR 67.50 S 68 35.00 MR 15.74 MR 75.00 S
69 45.83 MS 27.03 MS 67.50 S 69 37.50 MR 15.33 MR 67.50 S
70 54.17 MS 26.49 MS 68.18 S 70 44.17 MS 10.24 MR 75.00 S
71 54.17 MS 19.89 MR 65.91 S 71 52.50 MS 10.46 MR 75.00 S
72 50.83 MS 25.96 MS 68.18 S 72 50.83 MS 20.69 MR 70.00 S
73 48.33 MS 22.83 MS 65.91 S 73 48.33 MS 15.27 MR 67.50 S
74 50.00 MS 23.87 MS 70.45 S 74 45.00 MS 12.10 MR 57.50 MS
75 54.17 MS 20.63 MR 68.75 S 75 43.33 MS 11.62 MR 63.64 MS
76 59.17 S 14.55 MR 65.00 MS 76 45.83 MS 8.78 MR 55.00 MS
77 43.33 MS 19.86 MR 65.00 MS 77 54.17 MS 21.53 MR 62.50 MS
78 66.67 S 54.49 S 70.83 S 78 54.17 MS 22.14 MS 72.50 S
79 45.00 MS 30.83 S 67.31 S 79 71.67 S 23.73 MS 70.00 S
80 78.33 S 58.12 S 69.23 S 80 51.67 MS 19.91 MR 65.00 MS
81 33.33 MR 26.40 MS 70.83 S 81 32.50 MR 10.24 MR 63.64 MS
82 47.50 MS 21.07 MR 75.00 S 82 56.03 S 63.71 S 68.18 S
83 42.50 MS 24.60 MS 75.00 S 83 35.83 MR 7.77 MR 55.00 MS

Table 4

Simple correlations among different screening methods of FHB resistance of 186 homozygous lines with Fhb1 gene"

处理
Treatment
土表接种(徐州)
Soil surface inoculation
(in Xuzhou)
单花滴住(徐州)
Single flower drip inoculation
(in Xuzhou)
自然发病(建阳)
Screening under natural condition (in Jianyang)
土表接种(徐州) Screening under natural condition (in Xuzhou) 1.00 0.53** 0.19*
单花滴住(徐州) Single flower drip inoculation (in Xuzhou) 1.00 0.20*
自然发病(建阳) Screening under natural condition (in Jianyang) 1.00

Table 5

Results of FHB resistance of Xumai DH9 and Xumai 17252 screening in the wheat regional trials of Jiangsu province and Huaibei science and enterprise consortium"

品种
Variety
2017-2018年度
Plant season of 2017-2018
2018-2019年度
Plant season of 2018-2019
2019-2020年度
Plant season of 2019-2020
严重度
Severity of disease
抗性评价
Evaluation of resistance
严重度
Severity of disease
抗性评价
Evaluation of resistance
严重度
Severity of disease
抗性评价
Evaluation of resistance
徐麦DH9 Xumai DH9 2.10 MR 2.53 MR 1.22 MR
徐麦17252 Xumai 17252 1.91 MR 1.15 R
苏麦3号Sumai 3 (CK1) 1.27 R 1.45 R 1.10 R
扬麦158 Yangmai 158 (CK2) 2.15 MR 2.57 MR 1.98 MR
淮麦20 Huaimai 20 (CK3) 3.31 MS 3.37 MS 2.62 MS
矮抗58 Aikang 58 (CK4) 3.87 S 3.86 S 3.66 S

Table 6

Results of FHB resistance of Xumai DH9 and Xumai 17252 screening in the national regional trial of joint development of wheat variety"

品种名称
Variety
2018-2019年度Plant season of 2018-2019 2019-2020年度Plant season of 2019-2020
严重度
Severity of disease
病情指数
Disease index
抗性评价
Evaluation of resistance
严重度
Severity of disease
病情指数
Disease index
抗性评价
Evaluation of resistance
徐麦DH9 Xumai DH9 1.00 10.00 MR 1.47 36.72 MR
徐麦17252 Xumai 17252 1.6 40.08 MR
苏麦3号 Sumai 3 (CK1) 0.80 1.00 R 0.09 2.26 R
郑麦9023 Zhengmai 9023 (CK2) 2.00 20.00 MR 1.69 42.13 MR
郑麦0943 Zhengmai 0943 (CK3) 2.50 50.00 MS 2.69 67.32 MS
周麦18 Zhoumai 18 (CK4) 3.20 80.00 S
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