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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (12): 1760-1766.doi: 10.3724/SP.J.1006.2017.01760

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Construction of Waxy Maize opaque2 Near-isogenic Lines

ZHANG Xiao-Xing1, ZHU Hui1, ZHANG Dong-Min1, SONG Li-Ya2, ZHANG De-Gui1, WENG Jian-Feng1, HAO Zhuan-Fang1, and LI Ming-Shun1,*   

  1. 1 Institute of Crop Science, Chinese Academy of Agricultural Sciences / Key Laboratory of Crop Genetics and Breeding, Ministry of Agriculture, Beijing 100081, China; 2 Beijing Technology and Business University / Beijing Key Laboratory of Plant Resources Research and Development, Beijing 100048, China
  • Received:2017-03-09 Revised:2017-07-23 Online:2017-12-12 Published:2017-08-10
  • Supported by:

    This study was supported by the National Natural Science Foundation of China (31401390) and the China Agriculture Research System (CAR02-01).

Abstract:

In waxy maize, the opaque2 gene can increase the content of essential amino acids, such as lysine and tryptophan. Two high quality protein maize (QPM) inbred lines, CA339 and Lu 2548, were used as opaque2 gene donors. Twenty-five waxy maize with advantageous traits were used as receptors. Application of backcrossing and SSR marker-assisted selection in breeding can create waxy maize opaque2 near-isogenic lines. The results of molecular marker indicated that there were polymorphisms in different receptors and between the two donors. Lysine content of five sets of successfully created opaque2 near-isogenic lines was tested, showing that the content of lysine in o2w-NILs was 59.0%, 52.7%, 48.5%, 46.3%, and 61.9% higher than that in their recurrent parents, which was increased from 0.308%, 0.313%, 0.309%, 0.341%, 0.323% to 0.489%, 0.478%, 0.458%, 0.498%, and 0.522%, respectively. Using this method, opaque2 could be introgressed into multiple receptors with different genetic backgrounds to select near-isogenic lines with high lysine content and transparent phenotype, so as to improve nutritional and economic values of waxy maize.

Key words: Waxy maize, Opaque2 gene, Waxy gene, Near-isogenic lines, Lysine

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