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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (12): 1746-1759.doi: 10.3724/SP.J.1006.2017.01746

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Mapping QTLs for Phosphorus Efficiency at Tillering Stage under Different Phosphorus Levels in Barley (Hordeum vulgare L.)

HU De-Yi1, CAI Lu1, CHEN Guang-Deng1,*, ZHANG Xi-Zhou1,Chunji LIU2   

  1. 1 College of resources, Sichuan Agricultural University, Chengdu 611130, China; 2 CSIRO Agriculture, 306 Carmody Road, St Lucia, QLD 4067, Australia
  • Received:2016-12-12 Revised:2017-07-23 Online:2017-12-12 Published:2017-08-10
  • Supported by:

    This study was supported by the National Natural Science Foundation of China (31401377), the Key Technology R&D Program of Sichuan Province (2014NZ0008), and the Key Project of Education Department of Sichuan Province (14ZA0002).

Abstract:

Phosphorus (P) nutrition has close relationship with the quality and yield of barley and the genetic mechanism of P-efficient and variety improvement become hot research topics in recent years. In this study, we mapped quantitative trait loci (QTLs) for P-efficient traits using a recombinant inbred line (RIL) population derived from a cross between Baudin and CN4079. P-utilization efficiency (PUE), P-absorption efficiency (PAE), and dry weight (DW) of shoots and roots, as well as tiller number (TN) were evaluated in low-P (0.02 mmol L1 KH2PO4) and normal-P (0.2 mmol L1 KH2PO4) conditions. QTLs associated with these traits were mapped onto a barley linkage map and their candidate genes were predicted. Phenotyping results showed continuous variation and transgressive segregation in all traits tested. A total of 16 QTLs were detected on chromosomes 2H, 3H, and 5H under low-P and normal-P conditions, with explained phenotypic variance ranging from 14.1% to 28.5%. Three QTLs associated with PUE were mapped on 3H and their positive alleles were all from Baudin. Among them, Qspue.sau-3H.1 and Qrpue.sau-3H were located in common region with PAE loci Qspae.sau-3H and Qrpae.sau-3H, whereas Qspue.sau-3H.2 was very closely to Qtn.sau-3H controlling TN. Three QTLs for PAE were mapped on 5H, among which Qspae.sau-5H.2 and Qrpae.sau-5H were from CN4079. The two PAE loci were located in adjacent region intervals of PUE loci Qspue.sau-5H and Qrpue.sau-5H and DW loci Qsdw.sau-5H and Qrdw.sau-5H. In the four chromosomal fragments harboring P-efficiency loci, except for Qspue.sau-3H.1 that contained candidate genes in phosphoric acid metabolism and phospholipid metabolism, the remaining loci all contained genes in phosphate transporter and related genes in phosphoric acid metabolism and phospholipid metabolism.

Key words: Hordeum vulgare L., Tillering stage, Phosphorus efficiency, RIL, QTL mapping

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