欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2008, Vol. 34 ›› Issue (12): 2106-2111.doi: 10.3724/SP.J.1006.2008.02106

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

光敏核不育水稻农垦58S花粉败育过程中Ca2+-ATPase的变化

史银连1;邓芳1;曹剑波2;姚家玲1,*   

  1. 1华中农业大学生命科技学院; 2华中农业大学科技处电镜平台, 湖北武汉 430070
  • 收稿日期:2008-04-09 修回日期:2008-06-05 出版日期:2008-12-12 网络出版日期:2008-09-06
  • 通讯作者: 姚家玲
  • 基金资助:

    国家自然科学基金项目(2004CB117200)

Changes of Ca2+-ATPase in Photosensitive Genic Male-Sterile Rice Nongken 58S during Pollen Aborting

SHI Yin-Lian1,DENG Fang1,CAO Jian-Bo2,YAO Jia-Ling1*   

  1. 1 College of Life Science and Technology, Huazhong Agricultural University; 2 Electrical Microscope Public Laboratory, Huazhong Agricultural University, Wuhan 430070, Hubei, China
  • Received:2008-04-09 Revised:2008-06-05 Published:2008-12-12 Published online:2008-09-06
  • Contact: YAO Jia-Ling

PDF

1594

被引次数

4

摘要：

运用电镜细胞化学技术(铅盐沉淀法)，对长日照条件下光敏核不育水稻农垦58S和可育株系58N花药发育的不同时期进行了Ca2+-ATPase定位研究。结果表明，Ca2+-ATPase颗粒在可育与不育水稻的花药壁、花粉及药隔中的出现时间和数量具有明显差异。可育花药在花粉母细胞时期，药壁细胞内出现Ca2+-ATPase颗粒，至单核花粉后期，解体的绒毡层细胞和乌氏体表面分布大量的Ca2+-ATPase颗粒；与可育材料相比，不育花药在花粉发育的同时期，Ca2+-ATPase颗粒在药壁各层细胞内不仅出现的时间滞后，且分布的数量较少，到二核花粉时期(花粉已畸形空瘪)，药壁表皮细胞的液泡膜上、绒毡层细胞质中及乌氏体表面才出现明显的Ca2+-ATPase颗粒。可育花粉单核早期，花粉细胞内线粒体膜上有少量的Ca2+-ATPase分布；单核花粉中期，花粉外壁上有少量的Ca2+-ATPase分布；到单核花粉后期，Ca2+-ATPase颗粒大量分布在花粉外壁、内壁及细胞质膜和细胞核中。而不育花粉在单核花粉发育中都未见Ca2+-ATPase的分布，到花粉败育空瘪时才出现明显的Ca2+-ATPase颗粒，但数量较可育花粉同期同部位少。可育花药药隔细胞中Ca2+-ATPase在花粉母细胞减数分裂期就有分布，而不育花药到单核花粉早期药隔中才有少量的Ca2+-ATPase分布。由此推测，水稻不育系在其花粉发育中，由于细胞壁和质膜上Ca2+-ATPase颗粒出现时间滞后和数量减少影响到细胞膜钙泵将Ca2+由胞质向胞外转运的功能，致使胞质内Ca2+过多积累，导致花粉败育。

关键词: 光敏雄性核不育, 水稻, 花药发育, Ca2+-ATPase

Abstract:

TEM and lead nitrate were used to locate Ca2+-ATPase in the anther development of Photoperiod-sensitive Genic Male-sterile Rice Nongken 58S and normal fertile rice Nongken 58N. The results showed that the quantity and the producing time of Ca2+-ATPase in the anther wall, connective tissue and pollen of fertile and sterile rice were very different. Some of Ca2+-ATPase distributed on the epidermal cells of fertile anther at pollen mother cell stage. And there were some Ca2+-ATPase in different anther wall cells, the more on the Ubisch body during the development of the pollen. Comparatively, the less and the later-produced Ca2+-ATPase distributed in the different anther wall cells of the sterile anther at the same stage. There were visible Ca2+-ATPase in the vacuole membrane of epidermal cell, cytoplasm of remained tapetum cell and the surface of Ubisch bodies at the binucleate pollen stage. Abundant Ca2+-ATPase distributed on the exine, the intine, the membrane of the cytoplasm and the nucleus of fertile pollen at the late uninucleate pollen stage, but no Ca2+-ATPase distributed on the sterile pollen during the development of the anther except binucleate pollen stage. The quantity of Ca2+-ATPase in the sterile pollen was less than that in the fertile pollen at the binucleate pollen stage. There were most Ca2+-ATPase in the fertile connective tissue at pollen mother cell meiosis stage. Comparatively, a little Ca2+-ATPase distributed in the sterile connective tissue until the pre-uninucleate pollen stage. Based on the above results, we suppose that the reason of pollen abortion is the less and later-produced Ca2+-ATPase distribution on the sterile pollen wall and the plasma membrane during the sterile anther development. And Ca2+ pump does not work for little Ca2+-ATPase in the sterile rice, so that it makes pollen abortion with abundant Ca2+ in the cytoplasm.

Key words: Photoperiod-sensitive genic male-sterility, Rice (Oryza sativa L.), Anther development, Ca2+-ATPase

[1]Li S(李杉), Xing G-M(邢更妹), Cui K-R(崔凯荣), Yu C-H(余春红), Zhang X(张霞), Xu H-X(徐海霞), Wang Y-F(王亚馥). Ultracytochemical localization of calcium and ATPase activity on the 2,4-D induced somatic embryogenesis of Lycium barbarum L. Acta Biol Exp Sin (实验生物学报), 2003, 36(6): 414–420(in Chinese with English abstract)
[2]Meng X-H(孟祥红), Wang J-B(王建波), Li R-Q(利容千). Effect of photoperiod on Ca2+-ATPase distribution in photope-riod-sensitive cytoplasmic male-sterile wheat during anther de-velopment. Acta Bot Sin (植物学报), 2000, 42(5): 446–454(in Chinese with English abstract)
[3]Yu F, Ding Y L, Yin Z F. Ultracytochemical localization of Ca2+-ATPase during the Phloem Ganglion development in Phy- llostachys edulis. Acta Bot Boreali-Occident Sin, 2007, 27(4): 645–650
[4]Lin S-Z(林善枝), Zhang Z-Y(张志毅). Calcium effect and changes of Ca2+ -ATPase activities during chilling hardening in populus tomentosa cuttings. J Plant Physiol Mol Biol (植物生理与分子生物学学报), 2002, 28(6): 449–456(in Chinese with English abstract)
[5]Meng X-H(孟祥红), Wang J-B(王建波), Li R-Q(利容千). Effect of photoperiod on calcium distribution in photoperiod-sensitive cytoplasmic male-sterile wheat during anther development. Acta Bot Sin (植物学报), 2000, 42(1): 15–22(in Chinese with English abstract)
[6]Sun D-L(孙得兰), Wang H(王红), Jian L-C(简令成). The stabilization on the plasmalemma calcium-pump (Ca2+ -ATPase) in winter wheat seedlings by the cold-resistant agent CR-4. Chin Bull Bot (植物学通报), 1998, 15(2): 50–54(in Chinese with Eng-lish abstract)
[7]Wang T(王台), Tong Z(童哲). Changes in anther microstructure of the photoperiod-sensitive genic male sterile rice. Acta Agron Sin (作物学报), 1992, 18(2): 132–136(in Chinese with English abstract)
[8]Sun J(孙俊), Zhu Y-G(朱英国). The ultrastructure of pollen and anther wall in the development of Hubei photoperiod-sensitive genie male-sterile rice. Acta Agron Sin (作物学报), 1995, 21(3): 364–367(in Chinese with English abstract)
[9]Zhao S(赵莎), Yao J-L(姚家玲). Cytological study and PCD as-say on pollen development of photoperiod sensitive genic male sterile rice. J Huazhong Agric Univ (华中农业大学学报), 2007, 26(3): 283–288(in Chinese with English abstract)
[10]Tian H Q, Kuang A X, Musgrave M E, Russell S D. Calcium dis-tribution in fertile and sterile anthers of a photoperiod-sensitive genic male-sterile rice. Planta, 1998, 204: 183–192
[11]Lin J-J(林建军), Wei Y-Z(魏幼璋). The fine tuning system of in-tracellular Ca2+ in plant-Ca2+-ATPase. Chin Bull Bot (植物学通报), 2001, 18(2): 190–196(in Chinese with English abstract)
[12]Li R-Q(利容千), Zhu Y-G(朱英国), Meng X-H(孟祥红), Wang J-B(王建波). The distribution of calcium in the pollen and connective tissue of Honglian-Yuetai cytoplasmic male sterile rice. Acta Agron Sin (作物学报), 2001, 27(2): 230–235(in Chinese with English abstract)
[13]Hu S-Y(胡适宜). Embryology of Angiosperms (被子植物胚胎学). Beijing: People's Education Press, 1982. pp 49–50 (in Chi-nese)
[1] 田甜, 陈丽娟, 何华勤. 基于Meta-QTL和RNA-seq的整合分析挖掘水稻抗稻瘟病候选基因[J]. 作物学报, 2022, 48(6): 1372-1388.
[2] 郑崇珂, 周冠华, 牛淑琳, 和亚男, 孙伟, 谢先芝. 水稻早衰突变体esl-H5的表型鉴定与基因定位[J]. 作物学报, 2022, 48(6): 1389-1400.
[3] 周文期, 强晓霞, 王森, 江静雯, 卫万荣. 水稻OsLPL2/PIR基因抗旱耐盐机制研究[J]. 作物学报, 2022, 48(6): 1401-1415.
[4] 郑小龙, 周菁清, 白杨, 邵雅芳, 章林平, 胡培松, 魏祥进. 粳稻不同穗部籽粒的淀粉与垩白品质差异及分子机制[J]. 作物学报, 2022, 48(6): 1425-1436.
[5] 颜佳倩, 顾逸彪, 薛张逸, 周天阳, 葛芊芊, 张耗, 刘立军, 王志琴, 顾骏飞, 杨建昌, 周振玲, 徐大勇. 耐盐性不同水稻品种对盐胁迫的响应差异及其机制[J]. 作物学报, 2022, 48(6): 1463-1475.
[6] 杨建昌, 李超卿, 江贻. 稻米氨基酸含量和组分及其调控[J]. 作物学报, 2022, 48(5): 1037-1050.
[7] 杨德卫, 王勋, 郑星星, 项信权, 崔海涛, 李生平, 唐定中. OsSAMS1在水稻稻瘟病抗性中的功能研究[J]. 作物学报, 2022, 48(5): 1119-1128.
[8] 朱峥, 王田幸子, 陈悦, 刘玉晴, 燕高伟, 徐珊, 马金姣, 窦世娟, 李莉云, 刘国振. 水稻转录因子WRKY68在Xa21介导的抗白叶枯病反应中发挥正调控作用[J]. 作物学报, 2022, 48(5): 1129-1140.
[9] 王小雷, 李炜星, 欧阳林娟, 徐杰, 陈小荣, 边建民, 胡丽芳, 彭小松, 贺晓鹏, 傅军如, 周大虎, 贺浩华, 孙晓棠, 朱昌兰. 基于染色体片段置换系群体检测水稻株型性状QTL[J]. 作物学报, 2022, 48(5): 1141-1151.
[10] 王泽, 周钦阳, 刘聪, 穆悦, 郭威, 丁艳锋, 二宫正士. 基于无人机和地面图像的田间水稻冠层参数估测与评价[J]. 作物学报, 2022, 48(5): 1248-1261.
[11] 陈悦, 孙明哲, 贾博为, 冷月, 孙晓丽. 水稻AP2/ERF转录因子参与逆境胁迫应答的分子机制研究进展[J]. 作物学报, 2022, 48(4): 781-790.
[12] 王吕, 崔月贞, 吴玉红, 郝兴顺, 张春辉, 王俊义, 刘怡欣, 李小刚, 秦宇航. 绿肥稻秆协同还田下氮肥减量的增产和培肥短期效应[J]. 作物学报, 2022, 48(4): 952-961.
[13] 巫燕飞, 胡琴, 周棋, 杜雪竹, 盛锋. 水稻延伸因子复合体家族基因鉴定及非生物胁迫诱导表达模式分析[J]. 作物学报, 2022, 48(3): 644-655.
[14] 陈云, 李思宇, 朱安, 刘昆, 张亚军, 张耗, 顾骏飞, 张伟杨, 刘立军, 杨建昌. 播种量和穗肥施氮量对优质食味直播水稻产量和品质的影响[J]. 作物学报, 2022, 48(3): 656-666.
[15] 王琰, 陈志雄, 姜大刚, 张灿奎, 查满荣. 增强叶片氮素输出对水稻分蘖和碳代谢的影响[J]. 作物学报, 2022, 48(3): 739-746.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2