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作物学报 ›› 2008, Vol. 34 ›› Issue (12): 2106-2111.doi: 10.3724/SP.J.1006.2008.02106

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

光敏核不育水稻农垦58S花粉败育过程中Ca2+-ATPase的变化

史银连1;邓芳1;曹剑波2;姚家玲1,*   

  1. 1华中农业大学生命科技学院; 2华中农业大学科技处电镜平台, 湖北武汉 430070
  • 收稿日期:2008-04-09 修回日期:2008-06-05 出版日期:2008-12-12 网络出版日期:2008-09-06
  • 通讯作者: 姚家玲
  • 基金资助:

    国家自然科学基金项目(2004CB117200)

Changes of Ca2+-ATPase in Photosensitive Genic Male-Sterile Rice Nongken 58S during Pollen Aborting

SHI Yin-Lian1,DENG Fang1,CAO Jian-Bo2,YAO Jia-Ling1*   

  1. 1 College of Life Science and Technology, Huazhong Agricultural University; 2 Electrical Microscope Public Laboratory, Huazhong Agricultural University, Wuhan 430070, Hubei, China
  • Received:2008-04-09 Revised:2008-06-05 Published:2008-12-12 Published online:2008-09-06
  • Contact: YAO Jia-Ling

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2290

被引次数

4

摘要：

运用电镜细胞化学技术(铅盐沉淀法)，对长日照条件下光敏核不育水稻农垦58S和可育株系58N花药发育的不同时期进行了Ca2+-ATPase定位研究。结果表明，Ca2+-ATPase颗粒在可育与不育水稻的花药壁、花粉及药隔中的出现时间和数量具有明显差异。可育花药在花粉母细胞时期，药壁细胞内出现Ca2+-ATPase颗粒，至单核花粉后期，解体的绒毡层细胞和乌氏体表面分布大量的Ca2+-ATPase颗粒；与可育材料相比，不育花药在花粉发育的同时期，Ca2+-ATPase颗粒在药壁各层细胞内不仅出现的时间滞后，且分布的数量较少，到二核花粉时期(花粉已畸形空瘪)，药壁表皮细胞的液泡膜上、绒毡层细胞质中及乌氏体表面才出现明显的Ca2+-ATPase颗粒。可育花粉单核早期，花粉细胞内线粒体膜上有少量的Ca2+-ATPase分布；单核花粉中期，花粉外壁上有少量的Ca2+-ATPase分布；到单核花粉后期，Ca2+-ATPase颗粒大量分布在花粉外壁、内壁及细胞质膜和细胞核中。而不育花粉在单核花粉发育中都未见Ca2+-ATPase的分布，到花粉败育空瘪时才出现明显的Ca2+-ATPase颗粒，但数量较可育花粉同期同部位少。可育花药药隔细胞中Ca2+-ATPase在花粉母细胞减数分裂期就有分布，而不育花药到单核花粉早期药隔中才有少量的Ca2+-ATPase分布。由此推测，水稻不育系在其花粉发育中，由于细胞壁和质膜上Ca2+-ATPase颗粒出现时间滞后和数量减少影响到细胞膜钙泵将Ca2+由胞质向胞外转运的功能，致使胞质内Ca2+过多积累，导致花粉败育。

关键词: 光敏雄性核不育, 水稻, 花药发育, Ca2+-ATPase

Abstract:

TEM and lead nitrate were used to locate Ca2+-ATPase in the anther development of Photoperiod-sensitive Genic Male-sterile Rice Nongken 58S and normal fertile rice Nongken 58N. The results showed that the quantity and the producing time of Ca2+-ATPase in the anther wall, connective tissue and pollen of fertile and sterile rice were very different. Some of Ca2+-ATPase distributed on the epidermal cells of fertile anther at pollen mother cell stage. And there were some Ca2+-ATPase in different anther wall cells, the more on the Ubisch body during the development of the pollen. Comparatively, the less and the later-produced Ca2+-ATPase distributed in the different anther wall cells of the sterile anther at the same stage. There were visible Ca2+-ATPase in the vacuole membrane of epidermal cell, cytoplasm of remained tapetum cell and the surface of Ubisch bodies at the binucleate pollen stage. Abundant Ca2+-ATPase distributed on the exine, the intine, the membrane of the cytoplasm and the nucleus of fertile pollen at the late uninucleate pollen stage, but no Ca2+-ATPase distributed on the sterile pollen during the development of the anther except binucleate pollen stage. The quantity of Ca2+-ATPase in the sterile pollen was less than that in the fertile pollen at the binucleate pollen stage. There were most Ca2+-ATPase in the fertile connective tissue at pollen mother cell meiosis stage. Comparatively, a little Ca2+-ATPase distributed in the sterile connective tissue until the pre-uninucleate pollen stage. Based on the above results, we suppose that the reason of pollen abortion is the less and later-produced Ca2+-ATPase distribution on the sterile pollen wall and the plasma membrane during the sterile anther development. And Ca2+ pump does not work for little Ca2+-ATPase in the sterile rice, so that it makes pollen abortion with abundant Ca2+ in the cytoplasm.

Key words: Photoperiod-sensitive genic male-sterility, Rice (Oryza sativa L.), Anther development, Ca2+-ATPase

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