• 作物遗传育种·种质资源·分子遗传学 •

用单片段代换系（SSSLs）研究水稻株高及其构成因素QTL加性及上位性效应

1. 1西南大学水稻研究所/农业部生物技术与作物品质改良重点开放实验室,重庆400716;2华南农业大学广东省植物分子育种重点实验室,广东广州510642
• 收稿日期:2008-05-09 修回日期:2008-09-16 出版日期:2009-01-12 网络出版日期:2008-11-17
• 通讯作者: 何光华
• 基金资助:

本研究由国家自然科学基金重点项目（30330370），西南大学博士点基金项目，农学与生物科技学院重点实验室开放基金项目资助

Analysis on Additive Effects and Epistasis Effects of QTL for Plant Height and Its Components Using Single Segment Substitution Lines(SSSLs)in Rice

ZHAO Fang-Ming,ZHANG Gui-Quan,ZENG Rui-Zhen,YANG Zeng-Lin,ZHU Hai-Tao,ZHONG Bing-Qiang,LIN Ying-Hua,HE Guang-Hua

1. 1Rice Research Institute, Southwest University/Key Laboratory of Biotechnology and Crop Quality Improvement,Ministry of Agriculture,Chongqing 400716,China;2 Guangdong Key Laboratory of Plant Molecular Breeding,South China Agricultural University, Guangzhou 510642,China
• Received:2008-05-09 Revised:2008-09-16 Published:2009-01-12 Published online:2008-11-17
• Contact: HE Guang-Hua

Abstract:

Plant height is a typical quantitative trait that is liable to be influenced by genetic backgrounds and environments. As a novel research material, single segment substitution lines and double segment pyramiding lines in rice will make QTL identification and epistasis analysis more accurate because of diminishing the interference of genetic backgrounds among plants. In this study, Detection of QTLs controlling plant height and its components and analysis of epistasis effects were done with 16 secondary single segment substitution lines and 15 double segment pyramiding lines derived from crossing of primary SSSLs by randomized blocks design. The main results showed that 11 QTLs were detected and distributed on chromosomes 4, 6, and 10, of which three QTLs controlling plant height, one QTL coffering length of the first inernode from the top, two QTLs harboring length of the second internode from the top, two QTLs for length of the third internode from the top and three QTL controlling length of the fourth internode from the top were included. Twenty-three digenic interactions were detected for plant height and its components, of which seven interactions occurred between two loci both not having main effects on the traits, and 16 interactions each involved one locus having a main effect at the single-locus level and another locus that did not show significant effect at the single-locus level. The results indicated that both additive effects of QTL and epistasis effects between QTLs were important genetic components. Efficiency of QTLs identification and epistasis effects analysis between QTLs could be improved using secondary single segment substitution lines and double segment pyramiding lines derived from crossing of primary single segment substitution lines.