欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2009, Vol. 35 ›› Issue (1): 57-65.doi: 10.3724/SP.J.1006.2009.00057

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

大豆倒伏性及其相关性状的QTL分析

周蓉1,2,王贤智2,陈海峰2,张晓娟2,单志慧2,吴学军2,蔡淑平2,邱德珍2,周新安2,吴江生1   

  1. 1华中农业大学植物科学技术学院, 湖北武汉430070; 2中国农业科学院油料作物研究所, 湖北武汉430062
  • 收稿日期:2008-05-07 修回日期:2008-07-16 出版日期:2009-01-12 网络出版日期:2008-11-17
  • 通讯作者: 周新安
  • 基金资助:

    本研究由国家自然科学基金项目（30671313），国家高技术研究发展计划（863计划）项目（2006AA1000104），国家“十一五”科技支撑计划项目（2006BAD01A04-3）资助

QTL Analysis of Lodging and Related Traits in Soybean

ZHOU Rong1,2,WANG Xian-Zhi2,CHEN Hai-Feng2,ZHANG Xiao-Juan2,SHAN Zhi-Hui2,WU Xue-Jun2,CAI Shu-Ping2,QIU De-Zhen2,ZHOU Xin-An2*,WU Jiang-Sheng1   

  1. 1College of Plant Science and Technology,Huazhong Agricultural University,Wuhan 430070,China;2 Oil Crops Research Institute,Chinese Academy of Agricultural Sciences,Wuhan 430062,China
  • Received:2008-05-07 Revised:2008-07-16 Published:2009-01-12 Published online:2008-11-17
  • Contact: ZHOU Xin-An

PDF

1858

被引次数

68

摘要：

利用来自中豆29×中豆32的165个重组自交系F10进行2年田间试验, 以复合区间作图法检测与大豆倒伏及形态性状有关的QTL。结果表明, 2年分别检测到25个和19个与大豆倒伏及茎杆性状和根系性状有关的QTL, 分布于A2、C1、C2、D1a、F、G、I和L连锁群, 可解释4.4%~50.1%的表型变异。在F连锁群上, 2年均检测到倒伏主效QTL(qLD-15-1)和株高主效QTL(qPH-15-2)；G连锁群和L连锁群上分别有1个主茎节数QTL和2个根重QTL在2个年份重复出现。在倒伏QTL的附近检测出株高、根重、茎叶重、茎粗、主茎节数和分枝数QTL, 表明植株地上部和地下部性状与抗倒性普遍关联；QTL定位结果与表型相关分析一致, 反映了这些形态性状表型相关的遗传特性。部分性状QTL存在共位性, 但是未在2个年份稳定表达。

关键词: 大豆, 倒伏, 茎杆性状, 根系性状, QTL

Abstract:

Lodging is an important agronomical trait in soybean, which has obvious negative effect on yield, seed quality and mechanical harvesting efficiency. The 165 recombinant inbred lines (RIL) derived from a cross between Zhongdou 29 and Zhongdou 32 were used in two years, with the composite interval mapping (CIM) method to identify the QTLs associated with lodging, plant height, stem diameter, nodes on main stem, branch number, shoot weight, root weight and stem intension. On linkage groups A2, C1, C2, D1a, F, G, I, and L, 25 and 19 QTLs for lodging and related traits were detected in 2006 and 2007, respectively, explaining 4.4–50.1% phenotypic variation. One QTL (qLD-15-1) of lodging and one QTL (qPH-15-2) of plant height on F linkage group were both detected in two years; one QTL of nodes on main stem and 2 QTLs of root weight were detected in two years on G and L linkage groups respectively. The positions of some QTLs of plant height, root weight, shoot weight, stem diameter, nodes on main stem and branch number were close to those of lodging, showing that the lodging resistance is associated with the characters in aerial part and underground portion, the results were consistent with the correlation analysis of phenotypic traits, showing the correlation of these morphological traits in genetics. The QTLs of some traits had the same positions, but were not both detected in two years.

Key words: Soybean, Lodging, Culm traits, Root traits, QTL

[1]Keller M, Karutz C, Schmid J E, Stamp P, Winzeler M, Keller B, Messmer M M. Quantitative trait loci for lodging resistance in a segregating wheat × spelt population. Theor Appl Genet, 1999, 98: 1171–1182
[2]Menchey E K, Aycock Jr M K. Anther-derived dihaploids for lodging improvement in tobacco. Crop Sci, 1998, 38: 698–701
[3]Tar’an B, Warkentin T, Somers D J, Miranda D, Vandenberg A, Blade S, Woods S, Bing D, Xue A, DeKoeyer D, Penner G. Quantitative trait loci for lodging resistance, plant height and partial resistance to mycosphaerella blight in field pea (Pisum sativum L.). Theor Appl Genet, 2003, 107: 1482–1491
[4]Inoue M, Gao Z S, Cai H W. QTL analysis of lodging resistance and related traits in Italian ryegrass (Lolium multiflorum Lam.). Theor Appl Genet, 2004, 109: 1576–1585
[5]Mansur L M, Orf J H, Chase K, Jarvik T, Cregan P B, Lark K G. Genetic mapping of agronomic traits using recombinant inbred lines of soybean. Crop Sci, 1996, 36: 1327–1336
[6]Lee S H, Bailey M A, Mian M A R, Carter Jr T E, Ashley D A, Hussey R S, Parrott W A, Boerma H R. Molecular markers associated with soybean plant height, lodging, and maturity across locations. Crop Sci, 1996, 36: 728–735
[7]Orf J H, Chase K, Jarvik T, Mansur L M, Cregan P B, Adler F R, Lark K G. Genetics of soybean agronomic traits: I. Comparison of three related recombinant inbred populations. Crop Sci, 1999, 39: 1642–1651
[8]Specht J E, Chase K, Macrander M, Graef G L, Chung J, Markwell J P, Germann M, Orf J H, Lark K G. Soybean response to water: A QTL analysis of drought tolerance. Crop Sci, 2001, 41: 493–509
[9]Chapman A, Pantalone V R, Ustun A, Allen F L, Landau-Ellis D, Trigiano R N, Gresshoff P M. Quantitative trait loci for agronomic and seed quality traits in an F2 and F4:6 soybean population. Euphytica, 2003, 129: 387–393
[10]Wu X-L(吴晓雷), Wang Y-J(王永军), He C-Y(贺超英), Chen S-Y(陈受宜), Gai J-Y(盖钧镒), Wang X-C(王学臣). QTL mapping of some agronomic traits of soybean. Acta Genet Sin(遗传学报), 2001, 28(10): 947–955(in Chinese with English abstract)
[11]Zhang W K, Wang Y J, Luo G Z, Zhang J S, He C Y, Wu X L, Gai J Y, Chen S Y. QTL mapping of ten agronomic traits on the soybean (Glycine max L. Merr.) genetic map and their association with EST markers. Theor Appl Genet, 2004, 108: 1131–1139
[12]Kabelka E A, Diers B W, Fehr W R, LeRoy A R, Baianu I C, You T, Neece D J, Nelson R L. Putative alleles for increased yield from soybean plant introductions. Crop Sci, 2004, 44: 784–791
[13]Wang D, Graef G L, Procopiuk A M, Diers B W. Identification of putative QTL that underlie yield in interspecific soybean backcross populations. Theor Appl Genet, 2004, 108: 458–467
[14]Brucher E, Niblack T, Kopisch-Obuch F J, Diers B W. The effect of rhg1 on reproduction of Heterodera glycines in the field and greenhouse and associated effects on agronomic traits. Crop Sci, 2005, 45: 1721–1727
[15]Kabelka E A, Carlson S R, Diers B W. Glycine soja PI 468916 SCN resistance loci’s associated effects on soybean seed yield and other agronomic traits. Crop Sci, 2006, 46: 622–629
[16]Guzman P S, Diers B W, Neece D J, Martin S K St, LeRoy A R, Grau C R, Hughes T J, Nelson R L. QTL associated with yield in three backcross-derived populations of soybean. Crop Sci, 2007, 47: 111–122
[17]Qiu L-J(邱丽娟), Chang R-Z(常汝镇). Descriptors and Data Standard for Soybean (Glycine spp.) (大豆种质资源描述规范和数据标准). Beijing: China Agriculture Press, 2006 (in Chinese)
[18]Gai J-Y(盖钧镒). Experimentation Methods (试验统计方法), 3rd edn. Beijing: China Agriculture Press, 2000. pp 248–252 (in Chinese)
[19]Ma Y-H(马育华). Foundation of Statistical Genetics and Plant Breeding (植物育种的数量遗传学基础). Jiangsu: Jiangsu Science & Technology Press, 1984. pp 442–445 (in Chinese)
[20]Song Q J, Marek L F, Shoemaker R C, Lark K G, Concibido V C, Delannay X, Specht J E, Cregan P B. A new integrated genetic linkage map of the soybean. Theor Appl Genet, 2004, 109: 122–128
[21]McCouch S R, Cho Y G, Yano M, Paul E, Blinstrub M, Morishima H, Kinoshita T. Report on QTL nomenclature. Rice Genet Newsl, 1997, 14: 11–13
[22]Wang H-L(王宏林), Yu D-Y(喻德跃), Wang Y-J(王永军), Chen S-Y(陈受宜), Gai J-Y(盖钧镒). Mapping QTL of soybean root weight with RIL population NJRIKY. Hereditas (遗传), 2004, 26(3): 333–336 (in Chinese with English abstract)
[23]Yang Z(杨喆), Guan R-X(关荣霞), Wang Y-J(王跃进), Liu Z-X(刘章雄), Chang R-Z(常汝镇), Wang S-M(王曙明), Qiu L-J(邱丽娟). Construction of genetic map and QTL analysis for some agronomic traits in soybean. J Plant Genet Resour (植物遗传资源学报), 2004, 5(4): 309–314 (in Chinese with English abstract)
[1] 陈玲玲, 李战, 刘亭萱, 谷勇哲, 宋健, 王俊, 邱丽娟. 基于783份大豆种质资源的叶柄夹角全基因组关联分析[J]. 作物学报, 2022, 48(6): 1333-1345.
[2] 胡文静, 李东升, 裔新, 张春梅, 张勇. 小麦穗部性状和株高的QTL定位及育种标记开发和验证[J]. 作物学报, 2022, 48(6): 1346-1356.
[3] 杨欢, 周颖, 陈平, 杜青, 郑本川, 蒲甜, 温晶, 杨文钰, 雍太文. 玉米-豆科作物带状间套作对养分吸收利用及产量优势的影响[J]. 作物学报, 2022, 48(6): 1476-1487.
[4] 王炫栋, 杨孙玉悦, 高润杰, 余俊杰, 郑丹沛, 倪峰, 蒋冬花. 拮抗大豆斑疹病菌放线菌菌株的筛选和促生作用及防效研究[J]. 作物学报, 2022, 48(6): 1546-1557.
[5] 于春淼, 张勇, 王好让, 杨兴勇, 董全中, 薛红, 张明明, 李微微, 王磊, 胡凯凤, 谷勇哲, 邱丽娟. 栽培大豆×半野生大豆高密度遗传图谱构建及株高QTL定位[J]. 作物学报, 2022, 48(5): 1091-1102.
[6] 李阿立, 冯雅楠, 李萍, 张东升, 宗毓铮, 林文, 郝兴宇. 大豆叶片响应CO2浓度升高、干旱及其交互作用的转录组分析[J]. 作物学报, 2022, 48(5): 1103-1118.
[7] 彭西红, 陈平, 杜青, 杨雪丽, 任俊波, 郑本川, 罗凯, 谢琛, 雷鹿, 雍太文, 杨文钰. 减量施氮对带状套作大豆土壤通气环境及结瘤固氮的影响[J]. 作物学报, 2022, 48(5): 1199-1209.
[8] 王好让, 张勇, 于春淼, 董全中, 李微微, 胡凯凤, 张明明, 薛红, 杨梦平, 宋继玲, 王磊, 杨兴勇, 邱丽娟. 大豆突变体ygl2黄绿叶基因的精细定位[J]. 作物学报, 2022, 48(4): 791-800.
[9] 李瑞东, 尹阳阳, 宋雯雯, 武婷婷, 孙石, 韩天富, 徐彩龙, 吴存祥, 胡水秀. 增密对不同分枝类型大豆品种同化物积累和产量的影响[J]. 作物学报, 2022, 48(4): 942-951.
[10] 杜浩, 程玉汉, 李泰, 侯智红, 黎永力, 南海洋, 董利东, 刘宝辉, 程群. 利用Ln位点进行分子设计提高大豆单荚粒数[J]. 作物学报, 2022, 48(3): 565-571.
[11] 周悦, 赵志华, 张宏宁, 孔佑宾. 大豆紫色酸性磷酸酶基因GmPAP14启动子克隆与功能分析[J]. 作物学报, 2022, 48(3): 590-596.
[12] 王娟, 张彦威, 焦铸锦, 刘盼盼, 常玮. 利用PyBSASeq算法挖掘大豆百粒重相关位点与候选基因[J]. 作物学报, 2022, 48(3): 635-643.
[13] 董衍坤, 黄定全, 高震, 陈栩. 大豆PIN-Like (PILS)基因家族的鉴定、表达分析及在根瘤共生固氮过程中的功能[J]. 作物学报, 2022, 48(2): 353-366.
[14] 张艳波, 王袁, 冯甘雨, 段慧蓉, 刘海英. 棉籽油分和3种主要脂肪酸含量QTL分析[J]. 作物学报, 2022, 48(2): 380-395.
[15] 张国伟, 李凯, 李思嘉, 王晓婧, 杨长琴, 刘瑞显. 减库对大豆叶片碳代谢的影响[J]. 作物学报, 2022, 48(2): 529-537.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2