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作物学报 ›› 2009, Vol. 35 ›› Issue (1): 57-65.doi: 10.3724/SP.J.1006.2009.00057

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

大豆倒伏性及其相关性状的QTL分析

周蓉1,2,王贤智2,陈海峰2,张晓娟2,单志慧2,吴学军2,蔡淑平2,邱德珍2,周新安2,吴江生1   

  1. 1华中农业大学植物科学技术学院, 湖北武汉430070; 2中国农业科学院油料作物研究所, 湖北武汉430062
  • 收稿日期:2008-05-07 修回日期:2008-07-16 出版日期:2009-01-12 网络出版日期:2008-11-17
  • 通讯作者: 周新安
  • 基金资助:

    本研究由国家自然科学基金项目（30671313），国家高技术研究发展计划（863计划）项目（2006AA1000104），国家“十一五”科技支撑计划项目（2006BAD01A04-3）资助

QTL Analysis of Lodging and Related Traits in Soybean

ZHOU Rong1,2,WANG Xian-Zhi2,CHEN Hai-Feng2,ZHANG Xiao-Juan2,SHAN Zhi-Hui2,WU Xue-Jun2,CAI Shu-Ping2,QIU De-Zhen2,ZHOU Xin-An2*,WU Jiang-Sheng1   

  1. 1College of Plant Science and Technology,Huazhong Agricultural University,Wuhan 430070,China;2 Oil Crops Research Institute,Chinese Academy of Agricultural Sciences,Wuhan 430062,China
  • Received:2008-05-07 Revised:2008-07-16 Published:2009-01-12 Published online:2008-11-17
  • Contact: ZHOU Xin-An

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1587

被引次数

68

摘要：

利用来自中豆29×中豆32的165个重组自交系F10进行2年田间试验, 以复合区间作图法检测与大豆倒伏及形态性状有关的QTL。结果表明, 2年分别检测到25个和19个与大豆倒伏及茎杆性状和根系性状有关的QTL, 分布于A2、C1、C2、D1a、F、G、I和L连锁群, 可解释4.4%~50.1%的表型变异。在F连锁群上, 2年均检测到倒伏主效QTL(qLD-15-1)和株高主效QTL(qPH-15-2)；G连锁群和L连锁群上分别有1个主茎节数QTL和2个根重QTL在2个年份重复出现。在倒伏QTL的附近检测出株高、根重、茎叶重、茎粗、主茎节数和分枝数QTL, 表明植株地上部和地下部性状与抗倒性普遍关联；QTL定位结果与表型相关分析一致, 反映了这些形态性状表型相关的遗传特性。部分性状QTL存在共位性, 但是未在2个年份稳定表达。

关键词: 大豆, 倒伏, 茎杆性状, 根系性状, QTL

Abstract:

Lodging is an important agronomical trait in soybean, which has obvious negative effect on yield, seed quality and mechanical harvesting efficiency. The 165 recombinant inbred lines (RIL) derived from a cross between Zhongdou 29 and Zhongdou 32 were used in two years, with the composite interval mapping (CIM) method to identify the QTLs associated with lodging, plant height, stem diameter, nodes on main stem, branch number, shoot weight, root weight and stem intension. On linkage groups A2, C1, C2, D1a, F, G, I, and L, 25 and 19 QTLs for lodging and related traits were detected in 2006 and 2007, respectively, explaining 4.4–50.1% phenotypic variation. One QTL (qLD-15-1) of lodging and one QTL (qPH-15-2) of plant height on F linkage group were both detected in two years; one QTL of nodes on main stem and 2 QTLs of root weight were detected in two years on G and L linkage groups respectively. The positions of some QTLs of plant height, root weight, shoot weight, stem diameter, nodes on main stem and branch number were close to those of lodging, showing that the lodging resistance is associated with the characters in aerial part and underground portion, the results were consistent with the correlation analysis of phenotypic traits, showing the correlation of these morphological traits in genetics. The QTLs of some traits had the same positions, but were not both detected in two years.

Key words: Soybean, Lodging, Culm traits, Root traits, QTL

[1]Keller M, Karutz C, Schmid J E, Stamp P, Winzeler M, Keller B, Messmer M M. Quantitative trait loci for lodging resistance in a segregating wheat × spelt population. Theor Appl Genet, 1999, 98: 1171–1182
[2]Menchey E K, Aycock Jr M K. Anther-derived dihaploids for lodging improvement in tobacco. Crop Sci, 1998, 38: 698–701
[3]Tar’an B, Warkentin T, Somers D J, Miranda D, Vandenberg A, Blade S, Woods S, Bing D, Xue A, DeKoeyer D, Penner G. Quantitative trait loci for lodging resistance, plant height and partial resistance to mycosphaerella blight in field pea (Pisum sativum L.). Theor Appl Genet, 2003, 107: 1482–1491
[4]Inoue M, Gao Z S, Cai H W. QTL analysis of lodging resistance and related traits in Italian ryegrass (Lolium multiflorum Lam.). Theor Appl Genet, 2004, 109: 1576–1585
[5]Mansur L M, Orf J H, Chase K, Jarvik T, Cregan P B, Lark K G. Genetic mapping of agronomic traits using recombinant inbred lines of soybean. Crop Sci, 1996, 36: 1327–1336
[6]Lee S H, Bailey M A, Mian M A R, Carter Jr T E, Ashley D A, Hussey R S, Parrott W A, Boerma H R. Molecular markers associated with soybean plant height, lodging, and maturity across locations. Crop Sci, 1996, 36: 728–735
[7]Orf J H, Chase K, Jarvik T, Mansur L M, Cregan P B, Adler F R, Lark K G. Genetics of soybean agronomic traits: I. Comparison of three related recombinant inbred populations. Crop Sci, 1999, 39: 1642–1651
[8]Specht J E, Chase K, Macrander M, Graef G L, Chung J, Markwell J P, Germann M, Orf J H, Lark K G. Soybean response to water: A QTL analysis of drought tolerance. Crop Sci, 2001, 41: 493–509
[9]Chapman A, Pantalone V R, Ustun A, Allen F L, Landau-Ellis D, Trigiano R N, Gresshoff P M. Quantitative trait loci for agronomic and seed quality traits in an F2 and F4:6 soybean population. Euphytica, 2003, 129: 387–393
[10]Wu X-L(吴晓雷), Wang Y-J(王永军), He C-Y(贺超英), Chen S-Y(陈受宜), Gai J-Y(盖钧镒), Wang X-C(王学臣). QTL mapping of some agronomic traits of soybean. Acta Genet Sin(遗传学报), 2001, 28(10): 947–955(in Chinese with English abstract)
[11]Zhang W K, Wang Y J, Luo G Z, Zhang J S, He C Y, Wu X L, Gai J Y, Chen S Y. QTL mapping of ten agronomic traits on the soybean (Glycine max L. Merr.) genetic map and their association with EST markers. Theor Appl Genet, 2004, 108: 1131–1139
[12]Kabelka E A, Diers B W, Fehr W R, LeRoy A R, Baianu I C, You T, Neece D J, Nelson R L. Putative alleles for increased yield from soybean plant introductions. Crop Sci, 2004, 44: 784–791
[13]Wang D, Graef G L, Procopiuk A M, Diers B W. Identification of putative QTL that underlie yield in interspecific soybean backcross populations. Theor Appl Genet, 2004, 108: 458–467
[14]Brucher E, Niblack T, Kopisch-Obuch F J, Diers B W. The effect of rhg1 on reproduction of Heterodera glycines in the field and greenhouse and associated effects on agronomic traits. Crop Sci, 2005, 45: 1721–1727
[15]Kabelka E A, Carlson S R, Diers B W. Glycine soja PI 468916 SCN resistance loci’s associated effects on soybean seed yield and other agronomic traits. Crop Sci, 2006, 46: 622–629
[16]Guzman P S, Diers B W, Neece D J, Martin S K St, LeRoy A R, Grau C R, Hughes T J, Nelson R L. QTL associated with yield in three backcross-derived populations of soybean. Crop Sci, 2007, 47: 111–122
[17]Qiu L-J(邱丽娟), Chang R-Z(常汝镇). Descriptors and Data Standard for Soybean (Glycine spp.) (大豆种质资源描述规范和数据标准). Beijing: China Agriculture Press, 2006 (in Chinese)
[18]Gai J-Y(盖钧镒). Experimentation Methods (试验统计方法), 3rd edn. Beijing: China Agriculture Press, 2000. pp 248–252 (in Chinese)
[19]Ma Y-H(马育华). Foundation of Statistical Genetics and Plant Breeding (植物育种的数量遗传学基础). Jiangsu: Jiangsu Science & Technology Press, 1984. pp 442–445 (in Chinese)
[20]Song Q J, Marek L F, Shoemaker R C, Lark K G, Concibido V C, Delannay X, Specht J E, Cregan P B. A new integrated genetic linkage map of the soybean. Theor Appl Genet, 2004, 109: 122–128
[21]McCouch S R, Cho Y G, Yano M, Paul E, Blinstrub M, Morishima H, Kinoshita T. Report on QTL nomenclature. Rice Genet Newsl, 1997, 14: 11–13
[22]Wang H-L(王宏林), Yu D-Y(喻德跃), Wang Y-J(王永军), Chen S-Y(陈受宜), Gai J-Y(盖钧镒). Mapping QTL of soybean root weight with RIL population NJRIKY. Hereditas (遗传), 2004, 26(3): 333–336 (in Chinese with English abstract)
[23]Yang Z(杨喆), Guan R-X(关荣霞), Wang Y-J(王跃进), Liu Z-X(刘章雄), Chang R-Z(常汝镇), Wang S-M(王曙明), Qiu L-J(邱丽娟). Construction of genetic map and QTL analysis for some agronomic traits in soybean. J Plant Genet Resour (植物遗传资源学报), 2004, 5(4): 309–314 (in Chinese with English abstract)
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