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作物学报 ›› 2009, Vol. 35 ›› Issue (7): 1268-1273.doi: 10.3724/SP.J.1006.2009.01268

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

大豆导入系群体芽期耐低温位点的基因型分析及QTL定位

蒋洪蔚1,2,李灿东1,2,刘春燕1,2,张闻博2,邱鹏程2,李文12福,高运来1,2,胡国华1,3*,陈庆山2,*   

  1. 黑龙江省农垦科研育种中心，黑龙江哈尔滨150090；2东北农业大学农学院，黑龙江哈尔滨150030；3高家大豆工程技术研究中心，黑龙江哈尔滨150050
  • 收稿日期:2008-10-30 修回日期:2009-03-23 出版日期:2009-07-12 网络出版日期:2009-05-19
  • 通讯作者: 胡国华, E-mail: Hugh757@vip.163.com, Tel: 0451-55199475; 陈庆山, E-mail: qshchen@126.com, Tel: 0451-55191945
  • 作者简介:E-mail: j444444@yahoo.cn
  • 基金资助:

    本研究由引进国际先进农业科学技术计划（948计划）项目（2006-G1(A)），国家高技术研究发展计划（863计划）项目（2006AA100104-3），黑龙江省博士后科研启动基金（LHK-04014）资助。

Genotype Analysis and QTL Mapping for Tolerance to Low Temperature in Germination by Introgression Lines in Soybean

JIANG Hong-Wei1,2,LI Can-Dong1,2,LIU Chun-Yan1,2,ZHANG Wen-Bo2,QIU Peng-Cheng2,LI Wen-Fu12,GAO Yun-Lai12,HU Guo-Hua13*,CHEN Qing-Shan2*   

  1. 1Land Reclamation Research & Breeding Centre of Heilongjiang,Harbin 150090,China;2College of Agriculture,Northeast Agricultural University,Harbin 150030 China;3 The National Research Center of Soybean Engineering and Technology,Harbin 150050,China
  • Received:2008-10-30 Revised:2009-03-23 Published:2009-07-12 Published online:2009-05-19
  • Contact: HU Guo-Hua, E-mail: Hugh757@vip.163.com, Tel: 0451-55199475; CHEN Qing-Shan, E-mail: qshchen@126.com, Tel: 0451-55191945
  • About author:E-mail: j444444@yahoo.cn

PDF

2019

被引次数

37

摘要：

利用美国大豆品种Clark (供体亲本)与主栽品种红丰11 (轮回亲本)所构建的回交导入系，经过严格的芽期耐低温筛选鉴定，得到46个在芽期耐低温性状上明显超过轮回亲本的导入系个体。利用这套选择群体结合随机对照群体和基因型分析，通过基于遗传搭车原理的卡方分析和单向方差分析方法，检测到分布于大豆10个连锁群的14个与大豆芽期耐低温相关的QTL。其中卡方分析检测到12个供体片段的超导入位点，对芽期耐低温性状表现为正效应。方差分析检测到5个位点，也表现为正效应，且其中Satt237、SOYPRP1和Satt540 3个位点是两种方法共同检测到的，应视为与耐低温直接相关的QTL。本研究旨在创建大豆芽期耐低温分子育种的检测方法平台，为大豆芽期耐低温研究提供有用的分子标记。

关键词: 大豆, 导入系, 芽期, 耐低温, QTL定位

Abstract:

Northeast of China is the main soybean production area. Low temperature is one of the main factors to reduce soybean yield and to limit soybean production spreading to north regions. Shortening the growth stage is considered as one way of solving the problem, but the effective way is to breed cultivars with low temperature to tolerance. The identification of low-temperature tolerance germplasm is the basis for soybean breeding. A set of backcross introgression lines were constructed with Hongfeng 11 as recurrent parent and Clark as donor parent, 46 individuals were screened out, which were more tolerant than recurrent parent Hongfeng 11 under low temperature condition. Fifty individuals from BC1F4 were used as control (random population). Fourteen QTLs on ten linkage groups for tolerance to low temperature in germination were detected with genotype analysis by chi-test and one-way ANOVA. Twelve excessive-introgression alleles from donor parent with positive effect were acquired by chi-square and 5 alleles by ANOVA. Satt237, SOYPRP1, and Satt540 were detected by the two methods, so the three loci should be the main effect QTL and more believable. Introgression lines were used for mapping QTL related with tolerance to low temperature in germination, and the molecular mechanism could be studied in the further research on the platform established in this study for molecular assisted breeding.

Key words: Soybean, Introgression lines, Germination stage, Low temperature tolerance, QTL mapping

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