欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2009, Vol. 35 ›› Issue (12): 2218-2224.doi: 10.3724/SP.J.1006.2009.02218

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

甘薯IbNPR1全长cDNA序列的分离与表达特性分析

陈观水1,周以飞2,林生1,张铮1,潘大仁1,*   

  1. 1福建农林大学生命科学学院，福建福州350002；2福建农林大学作物科学学院，福建福州350002
  • 收稿日期:2008-12-31 修回日期:2009-06-25 出版日期:2009-12-10 网络出版日期:2009-09-10
  • 通讯作者: PAN Da-Ren; E-mail: pandaren@yahoo.com.cn; Tel: +86 59183798876

Isolation and Characterization of IbNPR1 Gene from Sweet Potato（Ipomoea batatas）

CHEN Guan-Shui1,ZHOU Yi-Fei2,LIN Sheng1,ZHANG Zheng1,PAN Da-Ren1,*   

  1. 1College of Life Science，Fujian Agriculture and Forestry University，Fuzhou 35002，China，College of Crop Science，Fujian Agriculture and Forestry University，Fuzhou 350002，China
  • Received:2008-12-31 Revised:2009-06-25 Published:2009-12-10 Published online:2009-09-10
  • Contact: PAN Da-Ren; E-mail: pandaren@yahoo.com.cn; Tel: +86 59183798876
  • Supported by:

    This study was supported by Fujian Province Natural Science Foundation (2006J0059) and Youth Foundation of Fujian Agriculture and Forestry University(08B12)

PDF

1462

被引次数

7

摘要：

在植物系统获得性抗性(SAR)中，NPR1蛋白是水杨酸介导的基因表达中关键调控因子。本研究以青农2号为试验材料，利用同源序列法和RACE技术分离甘薯SAR 途径的主要抗病信号元件NPR1 (none expresser of PR gene)的全长cDNA序列。序列分析表明，IbNPR1基因全长2 353 bp，包含一个编码586个氨基酸残基的开放阅读框，包含有类似拟南芥NPR1蛋白中的BTB/POZ和锚蛋白重复氨基酸序列结构域。聚类分析显示IbNPR1与来源于番茄的NPR1基因关系最近。Southern杂交及半定量RT-PCR分析表明，甘薯NPR1基因属于低拷贝基因家族，表达模式为组成型表达，并且SA能提高其表达水平。由该结果推测，IbNPR1可能在甘薯抵御病原物的侵染中起重要的作用。

关键词: 甘薯, ibnpr1基因, 抗病, cDNA末端快速扩增技术

Abstract:

NPR1 (non-expressor of pathogenesis-related genes 1) protein is a key regulator of salicylic acid (SA)-mediated gene epression in systemic acquired resistance (SAR). By using homologous cloning and RACE (rapid amplification of cDNA ends) techniques, a full-length cDNA of IbNPR1 (Ipomoea batatas non-expressor of pathogenesis-related genes 1) was isolated from sweet potato var. Qingnong 2. The full length cDNA was 2 353 bp, including an ORF (open reading frame) putatively encoding a polypeptide of 586 amino acids residues with a predicted molecular mass of 64.851 kD. The deduced amino acid sequence shared structural features with known NPR1 (-like) proteins: ankyrin repeat and BTB/POZ. Furthermore, phylogenetic analysis showed IbNPR1 had the closest association with LeNPR1 from Lycopersicon esculentum. Southern-blot analysis revealed that the IbNPR1 belonged to low-copy gene family in sweet potato. Semi-quantitative RT-PCR analysis indicated that IbNPR1 was constitutively expressed in roots, stems and leaves. In addition, IbNPR1 could be induced by salicylic acid. The results suggest that IbNPR1 plays an important role in the response to pathogen infections in sweet potato.

Key words: Ipomoea batatas, IbNPR1 gene, Disease resistance, RACE

[1] Mou Z, Fan W, Dong X. Inducers of plant systemic acquired resistance regulator NPR1 function through redox changes. Cell 2003, 113: 935-944

[2] Johnson C, Boden E, Arias J. Salicylic acid and NPR1 induce the recruitment of trans-activating TGA factors to a defense gene promoter in Arabidopsis. Plant Cell, 2003, 15: 1846-1858

[3] Spoel S H, Koornnef A, Claessens S M C, Korzelius J P, Van Pelt J A, Mueller M J, Buchala A J, Metraus J P, Brown R, Kazan K, Van Loon L C, Dong X, Pieterse C M J. NPR1 modulates cross-talk between salicylate- and jasmonate-dependent defense pathways through a novel function in the cytosol. Plant Cell, 2003, 15:760-770

[4] Uquillas C, Letelier I, Blanco F, Jordana X, Holuigue L. NPR1-independent activation of immediate early salicylic acid-responsive genes in Arabidopsis. Mol Plant Microbe Interact, 2004, 17: 34-42

[5] Zhang H Z(张红志), Cai X Z(蔡新忠). Nonexpressor of pathogenesis-related genes 1 (NPR1): A key node of plant disease resistance signalling network. Chin J Biotechnol (生物工程学报), 2005, 21(4): 511-515 (in Chinese with English abstract)

[6] Cao H, Glazebrook J, Clarke J D, Volko S, Dong X. The Arabidopsis NPR1 gene that controls systemic acquired resistance encodes a novel protein containing ankyrin repeats. Cell, 1997, 88: 57-63

[7] Dong X. NPR1, all things considered. Curr Opin Plant Biol, 2004, 7: 547-552

[8] Nandi A, Kachroo P, Fukushige H, Hildebrand D F, Klessig D F, Shah J. Ethylene and jasmonic acid signaling affect the NPR1-independent expression of defense genes without impacting resistance to Pseudomonas syringae and Peronospora parasitica in the Arabidopsis ssi1 mutant. Mol Plant Microbe Interact, 2003, 16: 588-599

[9] Liu G, Holub E B, Alonso J M, Ecker J R, Fobert P R. An Arabidopsis NPR1-like gene, NPR4, is required for disease resistance. Plant J, 2005, 41: 304-318

[10] Liu Y, Schiff M, Marathe R, Dinesh-Kumar S P. Tobacco Rar1, EDS1 and NPR1/NIM1 like genes are required for N-mediated resistance to tobacco mosaic virus. Plant J, 2002, 30: 415-429

[11] Fitzgerald H A, Chern M S, Navarre R, Ronald P C. Overexpression of (At) NPR1 in rice leads to a BTH- and environment-induced lesion-mimic/cell death phenotype. Mol Plant Microbe Interact, 2004, 17: 140-151

[12] Lin W C, Lu C F, Wu J W, Cheng M L, Lin Y M, Yang N S, Black L, Green S K, Wang J F, Cheng C P. Transgenic tomato plants expressing the Arabidopsis NPR1 gene display enhanced resistance to a spectrum of fungal and bacterial diseases. Transgenic Res, 2004, 13: 567-581

[13] Chern M, Fitzgerald H A, Canlas P E, Navarre D A, Ronald P C. Overexpression of a rice NPR1 homolog leads to constitutive activation of defense response and hypersensitivity to light. Mol Plant Microbe Interact, 2005, 18: 511-520

[14] Wang X J(王旭静), Dou D L(窦道龙), Wang Z X(王志兴), Jia S R(贾士荣). Cloning full-length cDNA of GbNPR1 gene from Gossypium barbadense and its expression in transgenic tobacco. Sci Agric Sin (中国农业科学), 2006, 39(5): 886-894 (in Chinese with English abstract)

[15] Yuan Y, Zhong S, Li Q, Zhu Z, Lou Y, Wang L, Wang J, Wang M, Li Q, Yang D, He Z. Functional analysis of rice NPR1-like genes reveals that OsNPR1/NH1 is the rice orthologue conferring disease resistance with enhanced herbivore susceptibility. Plant Biotechnol J, 2007, 5: 313-324

[16] Kinkema M, Fan W, Dong X. Nuclear localization of NPR1 is required for activation of PR gene expression. Plant Cell, 2000, 12: 2339-2350

[17] Pieterse C M, Van Loon L C. NPR1: The spider in the web of induced resistance signaling pathways. Curr Opin Plant Biol, 2004, 7: 456-464

[18] Rochon A, Boyle P, Wignes T, Fobert P R, Després C. The coactivator function of Arabidopsis NPR1 requires the core of its BTB/POZ domain and the oxidation of C-terminal cysteines. Plant Cell, 2006, 18: 3670-3685

[19] Després C, Chubak C, Rochon A, Clark R, Bethune T, Desveaux D, Fobert P R. The Arabidopsis NPR1 disease resistance protein is a novel cofactor that confers redox regulation of DNA binding activity to the basic domain/leucine zipper transcription factor TGA1. Plant Cell, 2003, 15: 2181-2191

[20] Food and Agriculture Organization. Production Year Book 2002, Vol. 54. Rome: Food and Agriculture Organization of the United Nations, 2002

[21] Guo J P(郭金平), Pan D R(潘大仁). PCR detection of nematode resistance in sweet potato. Acta Agron Sin (作物学报), 2002, 28: 167-169 (in Chinese with English abstract)

[22] Meura G, Budathab M, Guptab A D, Prakashc S, Kirti P B. Differential induction of NPR1 during defense responses in Brassica juncea. Physiol Mol Plant Pathol, 2006, 68: 128-137

[23] Zhang Y, Wang X, Cheng C, Gao Q, Liu J, Guo X. Molecular cloning and characterization of GhNPR1, a gene implicated in pathogen responses from cotton (Gossypium hirsutum L.). Biosci Rep, 2008, 28: 7-14

[24] Tang Y M(唐益苗), Zhang Z Y(张增艳), Xin Z Y(辛志勇). Isolation and characterization of NPR1 homolog gene TiNH1 in Thinopyrum intermedium. Sci Agric Sin (中国农业科学), 2007, 40(6): 1101-1107 (in Chinese with English abstract)

[25] Weigel R R, Bäuscher C, Pfitzner A J, Pfitzner U M. NIMIN-1, NIMIN-2 and NIMIN-3, members of a novel family of proteins from Arabidopsis that interact with NPR1/NIM1, a key regulator of systemic acquired resistance in plants. Plant Mol Biol, 2001, 46: 143-160

[26] Blanco F, Garretón V, Frey N, Dominguez C, Pérez-Acle T, Van der Straeten D, Jordana X, Holuigue L. Identification of NPR1-dependent and independent genes early induced by salicylic acid treatment in Arabidopsis. Plant Mol Biol, 2005, 59: 927-944
[1] 毛嘉琦, 黄朋雨, 赵佳佳, 郑兴卫, 武棒棒, 郝宇琼, 屈非, 刘成, 马朋涛, 郑军. 山西小麦品种白粉病抗性评价及抗病基因分子检测[J]. 作物学报, 2026, 52(6): 1669-1681.
[2] 蒋嘉卉, 江炳志, 刘冠明, 王章英, 唐朝臣. 紫肉甘薯品质性状的近红外光谱预测模型构建与优化[J]. 作物学报, 2026, 52(4): 1088-1102.
[3] 王懿涵, 李富昌, 刘意, 朱国鹏. 甘薯IbOPR2基因启动子克隆及调控因子的筛选[J]. 作物学报, 2026, 52(4): 1268-1276.
[4] 于永超, 刘明, 靳容, 赵鹏, 张强强, 王静, 朱晓亚, 唐忠厚. 甘薯高氮徒长的生理机制和转录组分析研究[J]. 作物学报, 2026, 52(3): 813-824.
[5] 杨璇, 李健康, 何婉洁, 李友军, 程相涵, 侯文邦. 硒肥对甘薯鲜切后褐变的影响及其机理解析[J]. 作物学报, 2026, 52(2): 578-589.
[6] 王粤生, 葛冬冬, 程兰斐, 陈春环, 王长有, 刘新伦, 李停栋, 邓平川, 吉万全, 赵继新. 小麦-华山新麦草二体异代换系16DH25-7的分子细胞遗传学及抗病性鉴定[J]. 作物学报, 2026, 52(2): 433-445.
[7] 苏爱国, 肖森林, 易红梅, 段赛茹, 王帅帅, 张如养, 邢锦丰, 李春辉, 孙轩, 徐瑞斌, 徐田军, 李志勇, 张勇, 王荣焕, 宋伟, 赵久然. 玉米穗腐病的抗性遗传研究进展与育种应用[J]. 作物学报, 2026, 52(1): 1-13.
[8] 张海燕, 解备涛, 董顺旭, 张立明, 段文学. 滴灌条件下不同水溶肥种类和配比对鲜食甘薯产量和品质的影响[J]. 作物学报, 2025, 51(9): 2485-2500.
[9] 尹雨萌, 王雁楠, 康志河, 乔守晨, 卞倩倩, 李亚蔚, 曹郭郑, 赵国瑞, 徐丹丹, 杨育峰. 甘薯谷胱甘肽S-转移酶基因IbGSTU7的克隆及功能分析[J]. 作物学报, 2025, 51(7): 1736-1746.
[10] 孟祥宇, 刁邓超, 刘雅睿, 李云丽, 孙玉晨, 吴玮, 赵雯, 汪妤, 吴建辉, 李春莲, 曾庆东, 韩德俊, 郑炜君. 小麦新品种西农877高产稳产的遗传特性解析[J]. 作物学报, 2025, 51(5): 1261-1276.
[11] 阳新月, 肖人滈, 张林茜, 唐铭均, 孙光燕, 杜康, 吕长文, 唐道彬, 王季春. 不同生育期涝渍对甘薯抗逆生理特性及产量形成的影响[J]. 作物学报, 2025, 51(3): 744-754.
[12] 霍如雪, 葛祥菡, 石嘉, 李雪蕊, 戴圣杰, 刘振宁, 李宗芸. 甘薯组氨酸激酶蛋白IbHK5响应干旱和盐胁迫的功能分析[J]. 作物学报, 2025, 51(3): 650-666.
[13] 王语新, 陈天羽, 翟红, 张欢, 高少培, 何绍贞, 赵宁, 刘庆昌. 甘薯激酶基因IbHT1的克隆及抗旱性功能鉴定[J]. 作物学报, 2025, 51(2): 301-311.
[14] 磨冬枝, 刘芃君, 徐桂娣, 陈泽炀, 谭雪明, 黄英金, 程建峰, 曾研华. 有机无机肥配施对甘薯产量、品质及硒吸收的影响[J]. 作物学报, 2025, 51(12): 3342-3356.
[15] 焦文娟, 白斌, 谢克莱·依拉木, 张飞飞, 贾秋珍, 耿洪伟, 程宇坤. 295份国内外小麦种质资源条锈病抗性评价及抗病基因分子检测[J]. 作物学报, 2025, 51(11): 2886-2898.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2