氮硫肥配施对小麦籽粒谷蛋白大聚合体含量及粒度分布的影响

doi:10.3724/SP.J.1006.2011.01060

摘要/Abstract

摘要： 以优质小麦品种山农15为材料，研究了氮硫肥配施对小麦籽粒谷蛋白大聚合体(GMP)含量、高分子量谷蛋白亚基(HMW-GS)含量、低分子量谷蛋白亚基(LMW-GS)含量和谷蛋白大聚合体(GMP)粒度分布的调控效应。结果表明，在一定范围内，增施氮肥可提高籽粒GMP含量、高分子量谷蛋白亚基(HMW-GS)含量和低分子量谷蛋白亚基(LMW-GS)含量。增施硫肥对籽粒GMP含量无显著影响，但降低了HMW-GS含量和D区LMW-GS含量，提高了B区和C区LMW-GS含量。增施氮肥和硫肥均降低小粒径(d < 12 µm)GMP颗粒体积和表面积百分比，提高大粒径(d ≥ 12 µm) GMP颗粒体积百分比和表面积百分比，但对GMP颗粒数目百分比无显著影响。相关性分析显示，C区LMW-GS含量与小粒径GMP颗粒体积百分和表面积百分比均呈显著负相关。说明增施氮肥能改变籽粒GMP的绝对含量，增施硫肥却改变籽粒GMP亚基的相对含量。増施氮肥和硫肥对大粒径GMP颗粒的体积及表面积分布均有正向效应；LMW-GS，特别是C区LMW-GS在大粒径GMP颗粒形成中起重要作用。

关键词: 小麦, 氮肥, 硫肥, HMW-GS, LMW-GS, 谷蛋白大聚合体(GMP)粒度分布

Abstract: The content and size distribution of glutenin macropolymer (GMP) play key roles in grain quality of wheat (Triticum aestivum L.). The aims of this study were to analyse the relationship between the content and size distribution of GMP and evaluate combined effects of nitrogen and sulphur fertilization on the content and size distribution of GMP in wheat grain. In the study, Shannong 15 was used in a field experiment with three nitrogen (N) application levels (120, 240, and 360 kg ha⁻¹) and three sulphur (S) application levels (0, 60, and 90 kg ha⁻¹). HMW-GS and LMW-GS were firstly separated using the sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and each of the subunits was then extracted before quantitative measurement by colorimetric analysis. The result showed that, in certain ranges, contents of GMP, HMW-GS, and LMW-GS were significantly increased with more application of nitrogen fertilizes. The increasing application of sulphur showed no significant effect on GMP content, but reduced contents of HMW-GS and D-LMW-GS and promoted contents of B-LMW-GS and C-LMW-GS. The content of small GMP particle (diameter less than 12 μm) was significantly reduced with the increase of nitrogen and sulphur fertilization, and the content of large GMP particle (diameter no less than 12 μm), tended to increase with the increase of nitrogen application and sulphur application. However, the distribution of GMP particle number was not affected by the nitrogen and sulphur treatments. According to correlation analysis, C-LMW-GS content was negatively correlated with the content of small GMP particle. These results suggested that increasing nitrogen application had the effect on the absolute content of GMP, while increasing sulphur application affected relative content of GMP. Nitrogen and sulphur fertilization have positive modulation on the content of large GMP particle, and C-LMW-GSplays an important role in the development of large GMP particle.

Key words: Wheat, Nitrogen fertilizer, Sulphur fertilizer, HMW-GS, LMW-GS, Glutenin macropolymer size distribution

蔡铁, 王振林, 尹燕枰, 李勇, 陈晓光, 王平, 陈二影, 郭俊祥, 倪英丽, 杨卫兵. 氮硫肥配施对小麦籽粒谷蛋白大聚合体含量及粒度分布的影响[J]. 作物学报, 2011, 37(06): 1060-1068.

CAI Tie, WANG Zhen-Lin, YIN Yan-Ping, LI Yong, CHEN Xiao-Guang, WANG Beng, CHEN Er-Ying, GUO Dun-Xiang, NI Yang-Li, YANG Wei-Bing. Combined Effects of Nitrogen and Sulphur Fertilization on Content and Size Distribution of Glutenin Macropolymer in Wheat Grain[J]. Acta Agron Sin, 2011, 37(06): 1060-1068.

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链接本文: https://zwxb.chinacrops.org/CN/10.3724/SP.J.1006.2011.01060

https://zwxb.chinacrops.org/CN/Y2011/V37/I06/1060

参考文献

[1]Don C, Lichtendonk W J, Plijter J J, Hamer R J. Understanding the link between GMP and dough: from glutenin particle in flour towards developed dough. J Cereal Sci, 2003, 38: 157−165
[2]Cornish G B, Bekes F, Allen H M, Allen H M, Martin D J. Flour proteins linked to quality traits in an Australian doubled haploid wheat population. Aust J Agric Res, 2001, 52: 1339−1348
[3]Gupta R B, Khan K, MacRitchie, F. Biochemical basis of flour properties in bread wheat: effects of variation in the quantity and size distribution of polymeric protein. J Cereal Sci, 1993, 18: 23−41
[4]Steffolani M E, Perez G T, Ribotta P D, Puppo M C, Leon A E. Effect of transglutaminase on properties of glutenin macropolymerand dough rheology. J Cereal Sci, 2008, 85: 39−43
[5]Nagamine T, Kai Y, Takayama T, Yanagisawa T, Taya S. Allelic variation at the Glu-1 and Glu-3 loci in southern Japanese wheats, and its effects on gluten properties. J Cereal Sci, 2000, 32: 129−135
[6]Metakovsky E V, Wrigley C W, Bekes F, Gupta R B, Metakovskii E V. Gluten polypeptides as useful genetic markers of dough quality in Australian wheat. Aust J Agric Res, 1990, 41: 289−306
[7]Don C, Lichtendonk W, Plijter J J, Hamer R J. Glutenin macropolymer: a gel formed by gluten in particles. J Cereal Sci, 2003, 37: 1−7
[8]Don C, Lookhart G, Naeem H, MacRitchie F, Hamer R J. Heat stress and genotype affect the gluten in particles of the gluten in macropolymer-gel fraction. J Cereal Sci, 2005, 42: 69−80
[9]Don C, Mann G, Bekes F, Hamer R J. HMW-GS affects the properties of glutenin particles in GMP and thus flour quality. J Cereal Sic, 2006, 44: 127−130
[10]Sun H(孙辉), Yao D-N(姚大年), Li B-Y(李保云), Liu G-T(刘广田), Zhang S-Z(张树榛). Correlation between content of glutenin macropolymer (GMP) in wheat and baking quality. Chin Cereals Oils Assoc (中国粮油学报), 1998, 13(6): 13−16 (in Chinese with English abstract)
[11]Zhu J, Khan K. Characterization of glutenin protein fractions from sequential extraction of hard red spring wheats of different bread-making quality. Cereal Chem, 2004, 81: 681−685
[12]Sun H(孙辉), Yao D-N(姚大年), Li B-Y(李保云), Liu G-T(刘广田), Zhang S-Z(张树榛). Effects of genetic and environmental factors on the content of glutenin macropolymer. J Triticeae Crops (麦类作物学报), 2000, 20(2): 23−27 (in Chinese with English abstract)
[13]Weegels P L, Hamer R J, Scholfield J D. Functional properties of wheat glutenin. J Cereal Sci, 1996, 23: 1−18
[14]Popineau Y, Cornec M, Lefebvre J, Marchylo B. Influence of high molecular glutenin subunits on glutenin polymers and rheological properties of near-isogenic lines of wheat Sicco. J Cereal Sci, 1994, 19: 231−241
[15]Zhu J B, Khan K, Huang S, O’Brien L. Allelic variation at Glu-D1 locus for high molecular weight (HMW) glutenin subunits: quantification by multistacking SDS-PAGE of wheat grow under nitrogen fertilization. Cereal Chem, 1999, 76: 915−919
[16]Yue H-W(岳鸿伟), Qin X-D(秦晓东), Dai T-B(戴廷波), Jing Q(荆奇), Cao W-X(曹卫星), Jiang D(姜东). Effects of nitrogen rate on accumulations of HMW-GS and GMP in wheat grain. Acta Agron Sin (作物学报), 2006, 32(10): 1678−1683 (in Chinese with English abstract)
[17]Byers M, Bolton J. Effects of nitrogen and sulphur fertilizers on the yield N and S content and amino acid composition of the grain of spring wheat. J Sci Food Agric, 1979, 30: 251−263
[18]Wrigley C W, Du Cros D L, Archer M J, Downie P G, Roxburgh C M. The sulfur content of wheat endosperm proteins and its relevance to grain quality. Aust J Plant Physiol, 1980, 7: 755−766
[19]Wrigley C W, Du Cros D L, Fullington J G, Kasarda D D. Changes in polypeptide composition and grain quality due to sulfur deficiency in wheat. J Cereal Sci, 1984, 2: 15−24
[20]Zhao F J, Salmon S E, Withers P J A, Monaghan J M, Evans E J, Shewry P R, McGrath S P. Variation in the breadmaking quality and rheological properties of wheat in relation to sulphur nutrition under field conditions. J Cereal Sci, 1999, 30: 19−31
[21]Liang R-Q(梁荣奇), Zhang Y-R(张义荣), You M-S(尤明山), Mao S-F(毛善锋), Sun J-M(宋建民), Liu G-T(刘广田). Multi-stacking SDS-PAGE for wheat glutenin polymer and its relation to bread-making quality. Acta Agron Sin (作物学报), 2002, 28(5): 609−614 (in Chinese with English abstract)
[22]Cui Z-Q(崔志青), He D-X(贺德先), Cai T(蔡铁), Wang C-Y(王成雨), Wang G-C(王广昌), Meng F-Y(孟范玉), Han Z-J(韩占江), Li N-N(李娜娜), Wang Z-L(王振林). Effects of spraying ABA on glutenin fraction content and GMP size distribution in wheat grain. Sci Agric Sin (中国农业科学), 2010, 43(12): 2595−2602 (in Chinese with English abstract)
[23]Liang T-B (梁太波), Yin Y-P(尹燕枰), Cai R-G(蔡瑞国), Yan S-H(闫素辉), Li W-Y(李文阳), Geng Q-H(耿庆辉), Wang P(王平), Wu Y-H(邬云海), Li Y(李勇), Wang Z-L(王振林). HMW-GS accumulation and GMP size distribution in grains of Shannong 12 grown in different soil conditions. Acta Agron Sin (作物学报), 2008, 34(12): 2160−2167 (in Chinese with English abstract)
[24]Ni Y-L (倪英丽), Wang Z-L(王振林), Li W-Y(李文阳), Yan S-H(闫素辉), Yin Y-P(尹燕枰), Li Y(李勇), Wang P(王平), Chen X-G(陈晓光). Effects of phosphorus fertilizer on accumulation of high molecular weight glutenin subunits and glutenin macropolymer size distribution in wheat grain. Acta Agron Sin (作物学报), 2010, 36(6): 1055−1060 (in Chinese with English abstract)
[25]Weegels P L, van de Pijpekamp A M, Graveland A, Hamer R J, Schofield J D. Depolymerisation and repolymerisation of wheat glutenin during dough processing: I. Relationships between glutenin macropolymer content and quality parameters. J Cereal Sci, 1996, 23: 103−111
[26]Don C, Lichtendonk W J, Plijter J J, Vliet T, Hamer R J. The effect of mixing on glutenin particle properties: aggregation factors that affect gluten function in dough. J Cereal Sci, 2005, 41: 69−83
[27]Zhu Y-J(朱云集), Shen X-S(沈学善), Li G-Q(李国强), Qu H-J(屈会娟), Guo T-C(郭天财), Dai T-B(戴廷波), Cao W-X(曹卫星). Effect of the combined application of sulfur and nitrogen fertilizers on yield and starch quality traits of soft-gluten wheat cultivar Yumai 50. J Triticeae Crops (麦类作物学报), 2007, 27(2): 271−275 (in Chinese with English abstract)
[28]Leustek T, Martin M N, Bick J A, Davies J D. Pathways and regulation of sulfur metabolism revealed through molecular and genetic studies. Annu Rev Plant Physiol Plant Mol Biol, 2000, 51: 141−165
[29]Leustek T, Saito K. Sulfate transport and assimilation in plants. Plant Physiol, 1999, 120: 637−644
[30]Koprivova A, Suter M, Opden Camp R, Brunold C, Kopriva S. Regulation of sulfate assimilation by nitrogen in Arabidopsis. Plant Physiol, 2000, 122: 737−746
[31]Stewart B A, Porter L K. Nitrogen sulfur relationships in wheat, corn and beans. Agron J, 1969, 61: 267−271
[32]Zhao S-P(赵首萍), Hu S-L(胡尚连), Li W-X(李文雄), Du J-Z(杜金哲). Effects of sulphur on grain protein content and storage protein content in spring wheat with different quality. Acta Agron Sin (作物学报), 2003, 29(6): 847−852 (in Chinese with English abstract)
[33]Zhu J B, Khan K. Characterization of nonnumeric and glutenin polymeric proteins of hard red spring wheats during grain development by multitasking SDS-PAGE and capillary zone electrophoresis. Cereal Chem, 1999, 76: 261−269
[34]Gupta R B, Batey I L, Mac Ritchie F. Relationship between protein composition and functional properties of wheat flour. Cereal Chem, 1992, 69: 125−131

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