作物学报 ›› 2012, Vol. 38 ›› Issue (01): 23-35.doi: 10.3724/SP.J.1006.2012.00023

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇



  1. 华南农业大学 / 国家植物航天育种工程技术研究中心, 广东广州510642
  • 收稿日期:2011-05-04 修回日期:2011-09-12 出版日期:2012-01-12 网络出版日期:2011-11-07
  • 通讯作者: 王慧, E-mail: wanghui@scau.edu.cn; 陈志强, E-mail: chenlin@scau.edu.cn
  • 基金资助:


Characterizations of a Mutant Gene hw-1(t) for Green-revertible Albino, High Tillering and Dwarf in Rice (Oryza sativa L.)

GUO Tao**,HUANG Xuan**,HUANG Yong-Xiang,LIU Yong-Zhu,ZHANG Jian-Guo,CHEN Zhi-Qiang*,WANG Hui*   

  1. South China Agricultural University / National Engineering Research Center of Plant Space Breeding, Guangzhou 510642, China
  • Received:2011-05-04 Revised:2011-09-12 Published:2012-01-12 Published online:2011-11-07
  • Contact: 王慧, E-mail: wanghui@scau.edu.cn; 陈志强, E-mail: chenlin@scau.edu.cn

摘要: 通过空间诱变从光身稻品种Francis的M2群体中发现一株叶色白化转绿、多分蘖矮秆突变体hfa-1hfa-1在三叶期之前完全白化,随后转绿。白化转绿表型受生长发育和温度调控。亚细胞结构观察发现hfa-1叶绿体发育异常抑制叶绿素合成,造成光合效率降低,产生白化表型。hfa-1的多分蘖表型是由于高节位分蘖芽激活所致,初步鉴定与苗期叶片IAA (吲哚乙酸)含量无关。hfa-1的矮生性则由节间长度缩短所致,与苗期GA (赤霉素)的合成和信号传导无关。遗传分析表明hfa-1的白化转绿、多分蘖矮秆表型受单隐性核基因hw-1(t)控制。利用hfa-1与粳稻品种02428杂交获得的F2群体将hw-1(t)定位在水稻第4染色体长臂上两个InDel标记HW27和HW7间46.9 kb的物理距离内,该区域有13个阅读框架,其中LOC_Os04g57320编码IMMUTANTS蛋白,推测为hw-1(t)的候选基因。

关键词: 水稻, 白化转绿, 多分蘖矮秆, hw-1(t), 精细定位

Abstract: A rice (Oryza sativa L.) mutant hfa-1 exhibiting green-revertible albino, high-tillering dwarf was detected from a M2 population of an American variety Francis by space mutagenesis. hfa-1 displayed distinctive albino before 3rd leaf stage but finally turned to normal green after 3rd leaf stage, and the expression of this phenotype was conditionally controlled by development and temperature. Examining ultrastructure and measuring chlorophyll content indicated that hfa-1 showed albino due to abnormal development of chloroplast, which resulted in inhibiting chlorophyll synthesis and decreasing photosynthetic efficiency. Moreover, the increased tiller number of hfa-1 was ascribed to initiate more higher-order tiller buds. Further endogenous hormones analysis demonstrated that the enhanced tillering capacity of hfa-1 might not result from inhibiting the synthesis of IAA. In addition, the dwarfism of hfa-1 was caused by shortening internodes and was independent of biosynthesis and signal transduction of GA. Genetic analysis indicated that the phenotype of green-revertible albino, high-tillering dwarf in hfa-1 was controlled by a recessive nucleic gene, namely hw-1(t). Using a large F2 mapping population derived from a cross between hfa-1 and an japonica rice variety, 02428, hw-1(t) was fine mapped into a 46.9 kb of physical distance between two InDel markers, HW27 and HW7 on chromosome 4, where 13 open reading frames were predicted. In the mapping interval LOC_Os04g57320 encoded a IMMUTANTS protein, which was the most properly candidate gene of hw-1(t).

Key words: Rice (Oryza sativa L.), Green-revertible albino, High-tillering dwarf, hw-1(t), Fine mapping

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