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作物学报 ›› 2012, Vol. 38 ›› Issue (07): 1178-1186.doi: 10.3724/SP.J.1006.2012.01178

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

水稻抗纹枯病QTL qSB-9TQ和抗条纹叶枯病基因Stv-bi的聚合育种

陈宗祥1，左示敏1,*，张亚芳1，朱俊凯1，王龙平1，冯凡1，马玉银2，潘学彪1,*   

  1. 1 扬州大学江苏省作物遗传生理重点实验室 / 植物功能基因组学教育部重点实验室，江苏扬州225009；2 扬州职业大学，江苏扬州225000
  • 收稿日期:2012-02-14 修回日期:2012-04-20 出版日期:2012-07-12 网络出版日期:2012-05-11
  • 通讯作者: 左示敏, 潘学彪, E-mail: shuidao@yzu.edu.cn, Tel: 0514-87972136
  • 基金资助:

    本研究由国家高技术研究发展计划(863计划)项目(2006AA10Z165, 2006AA10A103), 国家转基因生物新品种培育科技重大专项(2009ZX08001-014B)，国家自然科学基金项目(30900883)和江苏高校作物优势学科建设工程项目资助。

Rice Pyramiding Breeding using Sheath Blight Resistance QTL qSB-9TQ and Stripe Disease Resistance Gene Stv-bi

CHEN Zong-Xiang1,ZUO Shi-Min1,*,ZHANG Ya-Fang1,ZHU Jun-Kai1,WANG Long-Ping1,FENG Fan1,MA Yu-Yin2,PAN Xue-Biao1,*   

  1. 1 Key Laboratory of Crop Genetics and Physiology of Jiangsu Province / Key Laboratory of Plant Functional Genetics, Ministry of Education, Yangzhou University, Yangzhou 225009, China; 2 Yangzhou Polytechnic College, Yangzhou 225000, China
  • Received:2012-02-14 Revised:2012-04-20 Published:2012-07-12 Published online:2012-05-11
  • Contact: 左示敏, 潘学彪, E-mail: shuidao@yzu.edu.cn, Tel: 0514-87972136

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被引次数

8

摘要： 以携带抗纹枯病QTL qSB-9TQ的籼稻品种特青和携带抗条纹叶枯病基因Stv-bi的粳稻品种镇稻88为优良等位基因供体亲本，江苏省推广的粳稻品种武育粳3号和武粳15为受体亲本，分别杂交并连续回交。在回交及自交分离世代，利用开发的覆盖目标基因区间的双侧分子标记对目标基因进行辅助选择。至回交BC4F1世代，同一遗传背景2个回交方向的中选单株间聚合杂交，获得2个目标基因位点均纯合的聚合F3株系。条纹叶枯病抗性鉴定和纹枯病抗性接种鉴定结果表明，聚合株系对条纹叶枯病均表现抗病；以0~9级评级标准评价，聚合株系的纹枯病较相应的轮回亲本分别低1.1~1.6级和0.8~1.4级。结合回交低世代抗性鉴定结果分析，自行开发的分子标记对目标基因的辅助选择是有效的。讨论了抗纹枯病育种及分子标记辅助选择聚合育种的相关问题。

关键词: 分子标记辅助选择, 纹枯病, 条纹叶枯病, 聚合育种

Abstract: Indica rice cultivar Teqing and japonica rice cultivar Zhendao 88 were used as the donor parents with the sheath blight (SB) resistance QTL qSB-9TQ and the resistance gene Stv-bi, respectively. Commercial rice japonica cultivars, Wuyujing 3 and Wujing 15, were used as the receipt parents to cross and backcross with the donor parents, respectively. Flanked polymorphic markers to the two loci were developed and employed to select the target loci via marker assisted selection (MAS) in each backcross and self-cross progeny. Till backcross generation BC4F1, pyramiding was performed by crossing the selected individuals with different integression genes. At F3 generation, the pyramiding lines with homozygosity at both gene loci were obtained. Results of disease resistance evaluation indicated that both pyramiding lines were resistant to stripe disease, and increased resistance to SB with a reducing SB sating of 1.1–1.6 in Wuyujing 3 background and 0.8–1.4 in Wujing 15 background based on a “0–9” SB rating scale. Combining the results of resistance phenotype at the low backcrossing generation, we thought the markers developed in this study were efficient in selecting the two genes by MAS. Some questions associated with SB resistance breeding and pyramiding breeding via MAS were discussed.

Key words: Marker assisted selection, Sheath blight, Stripe disease, Pyramiding breeding

[1]Wang Y-Q(王艳青). Analysis on the dccurrence and development of rice diseases and insects in China. Chin Agric Sci Bull (中国农学通报), 2006, 22(2): 343-347 (in Chinese with English abstract)

[2]Lee F N, Rush M C. Rice sheath blight: a major rice disease. Plant Dis, 1983, 67: 829-832

[3]Liao H-N(廖皓年), Xiao L-S(肖陵生), Wang H-S(王华生). The occurring and developing history of sheath blight in rice. Guangxi Plant Prot (广西植保), 1997, (3): 35-38 (in Chinese)

[4]Lin Q-Y(林奇英), Xie L-H(谢联辉), Zhou Z-J(周仲驹), Xie L-Y(谢莉妍), Wu Z-J(吴祖建). Studies on rice stripe: I. Distribution of and losses caused by the disease. J Fujian Agric Coll (福建农学院学报), 1990, 19(4): 421-425 (in Chinese)

[5]Cheng Z-B(程兆榜), Yang R-M(杨荣明), Zhou Y-J(周益军), Diao C-Y(刁春友), Xiong R-Y(熊如意). New pattern of rice stripe disease occurred in Jiangsu rice planting region. Jiangsu Agric Sci (江苏农业科学), 2001, (1): 39-41 (in Chinese)

[6]Zuo S-M(左示敏), Zhang L(张丽), Wang H(王辉), Yin Y-J(殷跃军), Zhang Y-F(张亚芳), Chen Z-X(陈宗祥), Ma Y-Y(马玉银), Pan X-B(潘学彪). Prospect of the QTL-qSB-9Tq utilized in molecular breeding program of japonica rice against sheath blight. Acta Genet Sin (遗传学报), 2008, 35(8): 499-505

[7]Jia Y L, Liu G J, Costanzo S, Lee S, Dai Y T. Current progress on genetic interactions of rice with rice blast and sheath blight fungi. Front Agric China, 2009, 3: 231-239

[8]Zuo S-M(左示敏), Zhang Y-F(张亚芳), Chen Z-X(陈宗祥), Chen X-J(陈夕军), Pan X-B(潘学彪). Current progress on genetics and breeding in resistance to rice sheath blight. SCIENCE CHINA Life Sciences (中国科学•生命科学), 2010, 40(11): 1014-1023 (in Chinese with English abstract)

[9]Zuo S-M(左示敏), Yin Y-J(殷跃军), Zhang L(张丽), Zhang Y-F(张亚芳), Chen Z-X(陈宗祥), Pan X-B(潘学彪). Breeding value and further mapping of a QTL qSB-11 conferring the rice sheath blight resistance. Chin J Rice Sci (中国水稻科学), 2007, 21(2): 136-142 (in Chinese with English abstract)

[10]Tan C-X(谭彩霞), Ji X-M(纪雪梅), Yang Y(杨勇), Pan X-Y(潘兴元), Zuo S-M(左示敏), Zhang Y-F(张亚芳), Zou J-H(邹军煌), Chen Z-X(陈宗祥), Zhu L-H(朱立煌), Pan X-B(潘学彪). Identification and marker-assisted selection of two major quantitative genes controlling rice sheath blight resistance in backcross generations. Acta Genet Sin (遗传学报), 2005, 32(4): 399-405 (in Chinese with English abstract)

[11]Yin Y J, Zuo S M, Wang H, Chen Z X, Gu S L, Zhang Y F, Pan X B. Evaluation of the effect of qSB-9Tq involved in quantitative resistance to rice sheath blight using near-isogenic lines. Can J Plant Sci, 2009, 89: 731-737

[12]Hayano-Saito Y, Tsuji T, Fuji K, Saito K, Iwasaki M, Saito A. Localization of the rice stripe disease resistance gene, Stv- bi, by graphical genotyping and linkage analysis with molecular markers. Theor Appl Genet, 1998, 96: 1044-1049

[13]Maeda H, Sugisawa T, Nemoto H, Sunohara Y. QTL analysis for rice stripe resistance in the japanese upland rice kanto72. Breed Sci, 2004, 54: 19-26

[14]Ding X-L(丁秀兰), Jiang L(江玲), Liu S-J(刘世家), Wang C-M(王春明), Chen L-M(陈亮明), Cheng Z-B(程兆榜), Fan Y-J(范永坚), Zhou Y-J(周益军), Wan J-M(万建民). QTL analysis for rice stripe disease resistance gene using recombinant inbred lines (RILs) derived from crossing of kinmaze and DV85. Acta Genet Sin (遗传学报), 2004, 31(3): 287-292 (in Chinese with English abstract)

[15]Ding X-L(丁秀兰), Jiang L(江玲), Zhang Y-X(张迎信), Sun D-Z(孙黛珍), Zhai H-Q(翟虎渠), Wan J-M(万建民). Detection and analysis of QTL for resistance to stripe disease in rice, using backcross inbred lines. Acta Agron Sin (作物学报), 2005, 31(8): 1041-1046 (in Chinese with English abstract)

[16]Sun D-Z(孙黛珍), Jiang L(江玲), Zhang Y-X(张迎信), Cheng X-N(程遐年), Zhai H-Q(翟虎渠), Wan J-M(万建民). Analysis of quantitative trait loci for resistance to stripe disease in rice. Chin J Rice Sci (中国水稻科学), 2007, 21(1): 95-98 (in Chinese with English abstract)

[17]Pan X-B(潘学彪), Liang G-H(梁国华), Chen Z-X(陈宗祥), Zhang Y-F(张亚芳). Strategy on rice breeding against stripe disease in Jiangsu province. Jiangsu Agric Sci (江苏农业科学), 2005, (5): 22-23 (in Chinese)

[18]Hayano-Saito Y, Saito K, Nakamura S, Kawasaki S, Iwasaki M. Fine physical mapping of the rice stripe resistance gene locus, Stv-bi. Theor Appl Genet, 2000, 101: 59-63

[19]Ji Q, Lu J F, Chao Q, Gu M H, Xu M L. Delimiting a rice wide-compatibility gene S5n to a 50 kb region. Theor Appl Genet, 2005, 111: 1495-1503

[20]Murray M G, Thompson W F. Rapid isolation of high molecular weight plant DNA. Nucl Acids Res, 1980, 8: 4321-4326

[21]Pan X-B(潘学彪), Chen Z-X(陈宗祥), Xu J-Y(徐敬友), Tong Y-H(童蕴慧), Wang Z-B(王子斌), Pan X-Y(潘兴元). The effects of different methods of inoculation and investigation on genetic research of resistance to rice sheath blight. J Jiangsu Agric Coll (江苏农学院学报), 1997, 18(3): 27-32 (in Chinese with English abstract)

[22]Chen Z-X(陈宗祥), Zuo S-M(左示敏), Wang L-P(王龙平), Zhu J-K(朱俊凯), Zhang Y-F(张亚芳), Wang H(王辉), Ma Y-Y(马玉银), Pan X-B(潘学彪). Disease resistance evaluation to sheath blight of commercialized japonica rice cultivars around tenth five-year plan in Jiangsu province. J Yangzhou Univ (Agric Life Sci Edn)(扬州大学学报•农业与生命科学版), 2010, 31(2): 31-35 (in Chinese with English abstract)

[23]Zuo S-M(左示敏), Zhang Y-F(张亚芳), Yin Y-J(殷跃军),Chen Z-X(陈宗祥), Pan X-B(潘学彪). Establishment and improvement of inoculation technique and rating system in researching rice sheath blight resistance in field. J Yangzhou Univ (Agric Life Sci Edn)(扬州大学学报•农业与生命科学版), 2006, 27(4): 57-61 (in Chinese with English abstract)

[24]Chen X-W(陈学伟), Li S-G(李仕贵), MaY-Q(马玉清), Li H-Y(黎汉云), Zhou K-D(周开达), Zhu L-H(朱立煌). Marker-assisted selection and pyramiding for three blast resistance genes Pi-d(t)1, Pi-b, Pi-ta2 in rice. Chin J Biotechnol (生物工程学报), 2004, 20(5): 708-714 (in Chinese with English abstract)

[25]Liu W-G(柳武革), Wang F(王丰), Jin S-J(金素娟), Zhu X-Y(朱小源), Li J-H(李金华), Liu Z-R(刘振荣), Liao Y-L(廖亦龙), Zhu M-S(朱满山), Huang H-J(黄慧君), Fu F-H(符福鸿), Liu Y-B(刘宜柏). Improvement of rice blast resistance in TGMS Line by pyramiding of Pi-1 and Pi-2 through molecular marker-assisted selection. Acta Agron Sin (作物学报), 2008, 34(7): 1128-1136 (in Chinese with English abstract)

[26]Chen H-Q(陈红旗), Chen Z-X(陈宗祥), Ni S(倪深), Zuo S-M(左示敏), Pan X-B(潘学彪), Zhu X-D(朱旭东). Pyramiding three genes with resistance to blast by marker-assisted selection to improve rice blast resistance of Jin 23B. Chin J Rice Sci (中国水稻科学), 2008, 22(1): 23-27 (in Chinese with English abstract)

[27]Cao L-Y(曹立勇), Zhuang J-Y(庄杰云), Zhan X-D(占小登), Zheng K-L(郑康乐), Cheng S-H(程式华). Hybrid rice resistant to bacterial blight developed by marker assisted selection. Chin J Rice Sci (中国水稻科学), 2003, 17(2): 184-186 (in Chinese with English abstract)

[28]Sun R-K(孙荣科), Chen Q(陈乔), Li X-Q(李孝琼), Chen Y-Z(陈英之), Wei S-L(韦绍丽), Yang H-N(阳海宁), Zhang Y-X(张月雄), Li R-B(李容柏). Preliminary study on breeding hybrid rice by pyramiding brown planthopper resistance genes through molecular marker-assisted selection. Guangxi Agric Sci (广西农业科学), 2009, 40(6): 654-657 (in Chinese with English abstract)

[29]Ni D-H(倪大虎), Yi C-X(易成新), Yang J-B(杨剑波), Wang X-F(汪秀峰), Zhang Y(张毅), Zhang Q(章琦), Wang C-L(王春连), Zhao K-J(赵开军), Wang W-X(王文相), Li L(李莉). Pyramiding Pi9(t) and Xa23 genes by molecular marker-assisted selection. Mol Plant Breed (分子植物育种), 2007, 5(4): 491-496 (in Chinese with English abstract)

[30]Ni D-H(倪大虎), Yi C-X(易成新), Li L(李莉), Wang X-F(汪秀峰), Zhang Y(张毅), Zhao K-J(赵开军), Wang C-L(王春连), Zhang Q(章琦), Wang W-X(王文相), Yang J-B(杨剑波). Developing rice lines resistant to bacterial blight and blast with molecular marker-assisted selection. Acta Agron Sin (作物学报), 2008, 34(1): 100-105 (in Chinese with English abstract)

[31]Sang M-P(桑茂鹏), Jiang M-S(姜明松), Li G-X(李广贤), Yao F-Y(姚方印). Pyramiding Xa21 and fgr in rice by marker-assisted selection. Shandong Agric Sci (山东农业科学), 2009, (1): 4-7 (in Chinese with English abstract)

[32]Fu D, Chen L, Yu G H, Liu Y, Lou Q J, Mei H W, Xiong L, Li M-S, Xu X Y, Luo L J. QTL mapping of sheath blight resistance in a deep-water rice cultivar. Euphytica, 2011, 180: 209-218

[33]Wang Y, Pinson S R M, Fjellstrom R G, Tabien R E. Phenotypic gain from introgression of two QTL, qSB9-2 and qSB12-1, for rice sheath blight resistance. Mol Breed, 2011, DOI: 10.1007/s11032-011-9619-1 (Online first)

[34]Yin Y-J(殷跃军), Zuo S-M(左示敏), Wang H(王辉), Chen Z-X(陈宗祥), Ma Y-Y(马玉银), Zhang Y-F(张亚芳), Gu S-L(顾世梁), Pan X-B(潘学彪). Pyramiding effects of three quantitative trait loci for resistance to sheath blight using. Chin J Rice Sci (中国水稻科学), 2008, 22(4): 340-346 (in Chinese with English abstract)

[35]Pan X-B(潘学彪), Chen Z-X(陈宗祥), Zuo S-M(左示敏), Zhang Y-F(张亚芳), Wu X-J(吴旭江), Ma N(马宁), Jiang Q-X(江祺祥), Que J-H(阙金华), Zhou C-H(周春和). A new rice cultivar “Wulingjing 1” with resistance to rice stripe virus bred by marker assisted selection. Acta Agron Sin (作物学报), 2009, 35(10): 1851-1857(in Chinese with English abstract)
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